Article
Cell Biology
Jia Shi, Yi Yang, Ya-Nan Wang, Qing Li, Xue Xing, An-Ying Cheng, Xiao-Na Zhan, Jie Li, Gang Xu, Fan He
Summary: This study reveals the association between metabolism and the progression of vascular calcification in chronic kidney disease (CKD). High phosphate and uremic serum enhance mitochondrial respiration capacity in vascular smooth muscle cells (VSMCs), while glycolysis level remains unchanged. Suppression of cytochrome c oxidase (COX) activity attenuates vascular calcification.
CELL DEATH & DISEASE
(2022)
Review
Cell Biology
Icksoo Lee
Summary: This review focuses on resveratrol, (-)-epicatechin, and betaine and summarizes their effects on cytochrome c oxidase (COX). These compounds have been shown to improve mitochondrial biogenesis and COX function in various experimental models.
Article
Microbiology
Amber L. Grunow, Susan C. Carroll, Alicia N. Kreiman, Molly C. Sutherland
Summary: Heme trafficking is a fundamental biological process, and studying it has been challenging due to its tight regulation, cytotoxicity, and transient nature. The bacterial cytochrome c biogenesis pathways, especially System I, provide a model for understanding heme trafficking mechanisms. A detailed analysis of the WWD domain in CcmF reveals its interaction with heme and its role in attaching heme to apocytochrome c. This analysis also uncovers common mechanisms of heme interaction in different heme trafficking pathways.
Article
Plant Sciences
Alejandro Torrado, Macarena Iniesta-Pallares, Adrian Velazquez-Campoy, Consolacion Alvarez, Vicente Mariscal, Fernando P. Molina-Heredia
Summary: This study investigates the function and classification of cytochrome c(6)-like proteins in photosynthetic cyanobacteria, and provides new data on the phylogeny, genome localization, and functional properties of these proteins. The findings suggest that cytochrome c(6)-like proteins may interact with the cytochrome b(6)f complex in a similar way as cytochrome c(6), and cytochrome c(6)D can react with the specific copper-oxidase in heterocysts.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Multidisciplinary Sciences
Sibiao Yue, Lei Wang, George N. DeMartino, FangZhou Zhao, Yi Liu, Matthew H. Sieber
Summary: The regulation of cellular proteostasis is connected to the metabolic state of quiescent cells in vivo. The authors demonstrate that shifts in the ubiquitin-proteome system are linked to mitochondrial metabolic changes and respiratory quiescence. Cellular proteostasis defects and mitochondrial dysfunction play a role in infertility, cancer, and age-related diseases. Through a multi-organism approach, it is shown that dynamic shifts in the ubiquitin proteasome system drive mitochondrial remodeling during cellular quiescence.
NATURE COMMUNICATIONS
(2022)
Review
Biochemistry & Molecular Biology
Erika Fernandez-Vizarra, Cristina Ugalde
Summary: The lack of a consistent framework to explain the assembly of the mitochondrial respiratory chain complexes and supercomplexes has hindered progress in the field. The plasticity model, which proposes that the complexes can exist as individual entities or be associated in supercomplexes, is being questioned. Instead, a cooperative assembly model is argued to provide a better explanation for the observed phenomena in MRC biogenesis.
TRENDS IN BIOCHEMICAL SCIENCES
(2022)
Article
Multidisciplinary Sciences
Gizem Inak, Agnieszka Rybak-Wolf, Pawel Lisowski, Tancredi M. Pentimalli, Rene Juettner, Petar Glazar, Karan Uppal, Emanuela Bottani, Dario Brunetti, Christopher Secker, Annika Zink, David Meierhofer, Marie-Therese Henke, Monishita Dey, Ummi Ciptasari, Barbara Mlody, Tobias Hahn, Maria Berruezo-Llacuna, Nikos Karaiskos, Michela Di Virgilio, Johannes A. Mayr, Saskia B. Wortmann, Josef Priller, Michael Gotthardt, Dean P. Jones, Ertan Mayatepek, Werner Stenzel, Sebastian Diecke, Ralf Kuehn, Erich E. Wanker, Nikolaus Rajewsky, Markus Schuelke, Alessandro Prigione
Summary: Leigh syndrome (LS) is a severe neurometabolic disorder lacking effective models. This study found that LS carrying mutations in SURF1 gene exhibit impaired neuronal morphogenesis due to metabolic deficiencies.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Michael B. Powner, Glen Jeffery
Summary: Systemic glucose levels can be modulated through specific solar wavelengths that affect mitochondrial metabolism. Different wavelengths of light have opposing effects on mitochondrial respiration, with longer wavelengths increasing ATP production and shorter wavelengths suppressing metabolism. This study in a bumblebee model found that manipulating mitochondrial activity with specific wavelengths of light significantly altered circulating glucose concentration. This research contributes to our understanding of how light can affect systemic metabolism.
Article
Cell & Tissue Engineering
Yuling Han, Lei Tan, Ting Zhou, Liuliu Yang, Lucia Carrau, Lauretta A. Lacko, Mohsan Saeed, Jiajun Zhu, Zeping Zhao, Benjamin E. Nilsson-Payant, Filipe Tenorio Lira Neto, Clare Cahir, Alice Maria Giani, Jin Chou Chai, Yang Li, Xue Dong, Dorota Moroziewicz, Daniel Paull, Tuo Zhang, Soyeon Koo, Christina Tan, Ron Danziger, Qian Ba, Lingling Feng, Zhengming Chen, Aaron Zhong, Gilbert J. Wise, Jenny Z. Xiang, Hui Wang, Robert E. Schwartz, Benjamin R. tenOever, Scott A. Noggle, Charles M. Rice, Qibin Qi, Todd Evans, Shuibing Chen
Summary: This study demonstrates the use of human-induced pluripotent stem cells (hiPSCs) to link human genetics with viral infectivity. It identifies a cis-regulatory region of the NDUFA4 gene associated with susceptibility to Zika virus infection. Loss of NDUFA4 reduces sensitivity to multiple viral infections, and mechanistic studies reveal that loss/reduction of NDUFA4 induces mitochondrial stress and upregulation of interferon signaling.
Article
Multidisciplinary Sciences
Agnes Moe, Justin Di Trani, John L. Rubinstein, Peter Brzezinski
Summary: Energy conversion in aerobic organisms involves electron transfer from low-potential donors to dioxygen through the membrane-bound respiratory chain. The formation of supercomplex CIII2CIV1/2 facilitates electron transfer by two-dimensional diffusion of cyt. c, increasing QH(2):O-2 oxidoreductase activity and suggesting a regulatory role for supercomplex formation in the respiratory chain.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Cell Biology
Sue Ellen Brand, Martha Scharlau, Lois Geren, Marissa Hendrix, Clayre Parson, Tyler Elmendorf, Earl Neel, Kaila Pianalto, Jennifer Silva-Nash, Bill Durham, Francis Millett
Summary: Cytochrome c (Cc) has undergone accelerated evolution from anthropoid primates to humans, with several amino acid changes occurring near the binding site of the Cc:CcO complex. These changes affect the dissociation constant and the role of phosphorylation in regulating mitochondrial electron transport and membrane potential.
Article
Optics
S. Alekseenko, S. D. Solnushkin, V. N. Chikhman
Summary: In this study, an algorithm and software were developed to estimate the optical density as a measure of neural activity in multilayered brain structures. A method and program algorithm were also developed for quantitatively evaluating the optical density in layers of neural structures from histological brain preparation images. The practical significance of this research lies in its ability to reconstruct the functional activity of curved layers in different brain structures and investigate and simulate the organization patterns of the neural networks in the human brain.
JOURNAL OF OPTICAL TECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Abbas Abou-Hamdan, Roman Mahler, Philipp Grossenbacher, Olivier Biner, Dan Sjostrand, Martin Lochner, Martin Hogbem, Christoph von Ballmoos
Summary: Superoxide anion, produced in large amounts in enzymatic and adventitious reactions, serves various cellular functions but can also lead to the production of more harmful species. Recent findings suggest that the membrane protein CybB from E. coli may function as a superoxide:quinone oxidoreductase.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2022)
Article
Multidisciplinary Sciences
Aline Mourao Ximenes, Pedro Senna Bittencourt, Valeria Nogueira Machado, Tomas Hrbek, Izeni Pires Farias
Summary: South American freshwater ichthyofauna is the most diverse on the planet, but its diversity is vastly underestimated. This study investigated lineage diversity within the Geophagus sensu stricto species group and identified several undescribed species threatened by human activities affecting their habitats. The importance of combining DNA and morphological data in biodiversity assessment studies in taxonomically diverse tropical biotas was also highlighted.
Article
Engineering, Electrical & Electronic
Luca Giannoni, Frederic Lange, Marija Sajic, Kenneth J. Smith, Ilias Tachtsidis
Summary: The study introduces a novel hyperspectral imaging system hNIR for monitoring and quantifying changes in cerebral hemoglobin oxygenation and cellular metabolism in animals. Validated on optical phantoms mimicking brain tissue, the system is applied in vivo on mice to map microvascular levels of cortical hemodynamics and metabolism. This demonstrates the potential of hNIR as a powerful tool for simultaneous mapping and quantification of cerebral physiology using visible and NIR light.
IEEE JOURNAL OF SELECTED TOPICS IN QUANTUM ELECTRONICS
(2021)
Review
Cell Biology
Samantha A. Swenson, Courtney M. Moore, Jason R. Marcero, Amy E. Medlock, Amit R. Reddi, Oleh Khalimonchuk
Article
Endocrinology & Metabolism
Adam R. Wende, John C. Schell, Chae-Myeong Ha, Mark E. Pepin, Oleh Khalimonchuk, Hansjoerg Schwertz, Renata O. Pereira, Manoja K. Brahma, Joseph Tuinei, Ariel Contreras-Ferrat, Li Wang, Chase A. Andrizzi, Curtis D. Olsen, Wayne E. Bradley, Louis J. Dell'Italia, Wolfgang H. Dillmann, Sheldon E. Litwin, E. Dale Abel
Article
Cell Biology
Osiris Martinez-Guzman, Mathilda M. Willoughby, Arushi Saini, Jonathan Dietz, Iryna Bohovych, Amy E. Medlock, Oleh Khalimonchuk, Amit R. Reddi
JOURNAL OF CELL SCIENCE
(2020)
Article
Biochemistry & Molecular Biology
Heejeong Kim, Byeong Tak Jeon, Isaac M. Kim, Sydney J. Bennett, Carolyn M. Lorch, Martonio Ponte Viana, Jacob F. Myers, Caroline J. Trupp, Zachary T. Whipps, Mondira Kundu, Soonkyu Chung, Xinghui Sun, Oleh Khalimonchuk, Jaekwon Lee, Seung-Hyun Ro
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Plant Sciences
Ariel M. Hughes, Paul J. Zwack, Paul A. Cobine, Aaron M. Rashotte
Article
Biology
Xinyu Zhu, Aren Boulet, Katherine M. Buckley, Casey B. Phillips, Micah G. Gammon, Laura E. Oldfather, Stanley A. Moore, Scot C. Leary, Paul A. Cobine
Summary: The mitochondrial carrier family protein SLC25A3 transports copper and phosphate in mammals, while in Saccharomyces cerevisiae, the transport of these substrates is divided among orthologs PIC2 and MIR1. Evolutionary analysis revealed that PIC2-like and MIR1-like orthologs exist in all major eukaryotic supergroups, indicating an ancient gene duplication event. Structural modeling and mutagenesis identified residues involved in copper and phosphate transport, leading to the creation of a mouse SLC25A3 variant with selective copper transport capability. This study showcases the importance of using an evolutionary approach to uncover amino acids essential for substrate recognition by mitochondrial carrier family proteins.
Article
Multidisciplinary Sciences
Martonio Ponte Viana, Roman M. Levytskyy, Ruchika Anand, Andreas S. Reichert, Oleh Khalimonchuk
Summary: Remodeling of mitochondrial ultrastructure is critical for organelle physiology and apoptosis. OMA1, in addition to its role in mitochondrial division, is also required for maintaining intermembrane connectivity by dynamically associating with MICOS. This OMA1-MICOS relay is crucial for optimal bioenergetic output and cell apoptosis. Loss of OMA1 affects these functions but can be alleviated by a MICOS-emulating intermembrane bridge, highlighting OMA1's essential role in mitochondrial physiology.
Article
Cell Biology
Michelle Grace Acoba, Ebru S. Selen Alpergin, Santosh Renuse, Lucia Fernandez-del-Rio, Ya-Wen Lu, Oleh Khalimonchuk, Catherine F. Clarke, Akhilesh Pandey, Michael J. Wolfgang, Steven M. Claypool
Summary: The study revealed that SFXN1 plays a crucial role in mitochondrial respiratory chain by participating in heme and α-ketoglutarate metabolism, in addition to its known function as a mitochondrial serine transporter.
Article
Biochemistry & Molecular Biology
Xiao Cheng, Mohamed Sham Shihabudeen Haider Ali, Matthew Moran, Martonio Ponte Viana, Sarah L. Schlichte, Matthew C. Zimmerman, Oleh Khalimonchuk, Mark W. Feinberg, Xinghui Sun
Summary: The study found that the lncRNA Meg3 plays a role in obesity-induced insulin resistance by inducing cellular senescence of endothelial cells. Knockdown of Meg3 exacerbates the disease progression by promoting cellular senescence in hepatic endothelium.
Review
Cell Biology
Jonathan Dietz, Jennifer L. Fox, Oleh Khalimonchuk
Summary: Cellular iron homeostasis and mitochondrial iron homeostasis are interdependent, where mitochondria import iron to form clusters and heme, and supply the cell with heme for essential functions. Impairment in iron homeostasis can lead to various human diseases. Iron is stored and trafficked through carefully orchestrated processes intracellularly and within mitochondria.
Article
Multidisciplinary Sciences
Eva Nyvltova, Jonathan V. Dietz, Javier Seravalli, Oleh Khalimonchuk, Antoni Barrientos
Summary: Mitochondrial cytochrome c oxidase is an important enzyme involved in heme synthesis and copper transfer. Metallochaperones form macromolecular assemblies to regulate the biogenesis of catalytic assemblies and prevent the accumulation of harmful intermediates.
NATURE COMMUNICATIONS
(2022)
Article
Cell Biology
Antoineen J. White, Clare S. Harper, Erica M. Rosario, Jonathan V. Dietz, Hannah G. Addis, Jennifer L. Fox, Oleh Khalimonchuk, Laura L. Lackner
Summary: Num1 is a multifunctional protein involved in mitochondrial tethering and dynein anchoring. It is regulated by changes in metabolic state and plays a crucial role in maintaining mitochondrial morphology and function. Deficiency of Num1 leads to respiratory growth defects, and the dynein anchoring function mediated by Num1 is important for normal growth under respiratory conditions.
JOURNAL OF CELL SCIENCE
(2022)
Article
Chemistry, Multidisciplinary
Karen Acurio Cerda, Mark Kathol, Gunjan Purohit, Ehsan Zamani, Martha D. Morton, Oleh Khalimonchuk, Rajib Saha, Shudipto Konika Dishari
Summary: In this study, an inexpensive and abundant polymer lignin was functionalized with quaternary ammonium groups to create a cationic antimicrobial called QAL. QAL exhibited strong bacteriostatic and bacteriolytic effects against wild-type and antibiotic-resistant E. coli. The antimicrobial action of QAL was shown to be through increasing bacterial cell envelope thickness and causing damage to the inner membrane. The development of QAL as an efficient and renewable antimicrobial material from lignin can help address antibiotic resistance and promote biomass valorization.
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2023)
Article
Biochemical Research Methods
Gunjan Purohit, Martonio Ponte Viana, Oleh Khalimonchuk
Summary: Membrane contact sites play a critical role in intercompartmental communication. This article presents a protocol for engineering and validating a synthetic bridge between the inner and outer mitochondrial membranes to support the functioning of the MICOS complex. The use of this approach provides valuable insights into the function of MICOS and associated proteins.
Review
Oncology
Eva J. Ge, Ashley I. Bush, Angela Casini, Paul A. Cobine, Justin R. Cross, Gina M. DeNicola, Q. Ping Dou, Katherine J. Franz, Vishal M. Gohil, Sanjeev Gupta, Stephen G. Kaler, Svetlana Lutsenko, Vivek Mittal, Michael J. Petris, Roman Polishchuk, Martina Ralle, Michael L. Schilsky, Nicholas K. Tonks, Linda T. Vahdat, Linda Van Aelst, Dan Xi, Peng Yuan, Donita C. Brady, Christopher J. Chang
Summary: Copper is a essential nutrient with both beneficial and toxic properties to cells. Recent progress in transition metal signaling has led to new connections between researchers from different disciplines, with potential for translating basic research into clinical therapies for diseases. The relationship between copper and cancer is particularly important, with opportunities for leveraging disease vulnerabilities through copper-dependent signaling pathways.
NATURE REVIEWS CANCER
(2022)