Article
Cell Biology
Carol Dangelmaier, Satya P. Kunapuli
Summary: The study revealed that PI-3 Kinase plays a crucial role in platelet activation by regulating RASA3 and leading to Akt phosphorylation. LY294002 can inhibit Akt phosphorylation induced by certain stimuli at low concentrations, while higher concentrations may inhibit other pathways regulating Akt phosphorylation. Additionally, Protease-activated receptor 4 (PAR4) may cause Akt phosphorylation through distinct pathways compared to Protease-activated receptor 1 (PAR1).
Article
Biochemistry & Molecular Biology
Alexandra Yatine Lee, Sabrina Marie Christensen, Nhi Duong, Quoc-Anh Tran, Hou Mai Xiong, Jennifer Huang, Sarah James, Dimple Vallabh, George Talbott, Melanie Rose, Linh Ho
Summary: Sirtuin-3 (Sirt3) is a mitochondrial enzyme that regulates metabolic pathways and its expression is decreased in diabetes. This study found that the activator HNK increased adipogenesis and insulin signaling pathway, while the inhibitor 3-TYP had the opposite effect. These findings suggest that Sirt3 activation improves insulin resistance, while Sirt3 inhibition may worsen insulin resistance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Ruth R. Magaye, Feby Savira, Yue Hua, Xin Xiong, Li Huang, Christopher Reid, Bernard L. Flynn, David Kaye, Danny Liew, Bing H. Wang
Summary: Our study reveals a relationship between the PI3K/Akt-mTOR signaling cascade and collagen synthesis and myocyte hypertrophy induced by exogenous dhS1P in primary neonatal cardiac cells. In addition, PI3K inhibition also affects the gene expression related to S1P receptors and enzymes.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Zequn Lu, Ning Liu, Huoqing Huang, Yuan Wang, Tao Tu, Xing Qin, Xiaolu Wang, Jie Zhang, Xiaoyun Su, Jian Tian, Yingguo Bai, Huiying Luo, Bin Yao, Honglian Zhang
Summary: Human insulin-like growth factor-1 (IGF-1) and its analog Long R3 IGF-1 (LR3 IGF-1) were successfully recombinantly expressed and produced in the Pichia pastoris expression system by fusion with highly expressed xylanase XynCDBFV. The purified IGF-1 and LR3 IGF-1 displayed excellent bioactivity of cell proliferation compared to the standard IGF-1. Furthermore, high heterologous expression levels of the fusion proteins were achieved by fermentation in a 15-L bioreactor, which could be used for both clinical and scientific applications.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Biology
Alison L. Kearney, Dougall M. Norris, Milad Ghomlaghi, Martin Kin Lok Wong, Sean J. Humphrey, Luke Carroll, Guang Yang, Kristen C. Cooke, Pengyi Yang, Thomas A. Geddes, Sungyoung Shin, Daniel J. Fazakerley, Lan K. Nguyen, David E. James, James G. Burchfield
Summary: The study reveals that Akt phosphorylates IRS1 and IRS2, engaging in a negative feedback mechanism that limits the synthesis of PIP3 and plasma membrane-associated PI3K. This phosphorylation leads to the depletion of IRS1/2 from the plasma membrane, reducing their interaction with the insulin receptor.
Article
Biology
Hwan Bae, Thibault Viennet, Eunyoung Park, Nam Chu, Antonieta Salguero, Michael J. Eck, Haribabu Arthanari, Philip A. Cole
Summary: Akt is a protein kinase that plays a central role in metabolism and cancer. This study reveals the intensified autoinhibitory features of the R86A Akt mutant, which enhances the PH domain-kinase domain affinity. The key interaction network involving Arg86, Glu17, and Tyr18 is shown to control the conformation and activity of Akt, providing insights into the molecular basis of E17K Akt activation as an oncogenic driver.
Article
Biochemistry & Molecular Biology
Halima Meriem Issaadi, Zoltan Beni, Tunde Toth, Miklos Dekany, Tusty-Jiuan Hsieh, Gyorgy Tibor Balogh, Attila Hunyadi
Summary: The study utilized gamma-ray radiation for diversity-oriented synthesis of analogs of 20-hydroxyecdysone (20E) and isolated 14 ecdysteroids, including five new compounds. These new compounds showed stronger cytoprotective effects and capacity to promote Akt and AMPK phosphorylation in vitro compared to 20E.
BIOORGANIC CHEMISTRY
(2021)
Article
Gastroenterology & Hepatology
Ting Liu, Xiao-Li Xie, Xue Zhou, Sheng-Xiong Chen, Yi-Jun Wang, Lin-Ping Shi, Shu-Jia Chen, Yong-Juan Wang, Shu-Ling Wang, Jiu-Na Zhang, Shi-Ying Dou, Xiao-Yu Jiang, Ruo-Lin Cui, Hui-Qing Jiang
Summary: YB-1, overexpressed in HCC tissues, enhances sorafenib resistance through the PI3K/Akt pathway. Knockdown of YB-1 can improve the efficacy of sorafenib in vivo. The interaction between YB-1 and key proteins of the PI3K/Akt pathway contributes to sorafenib resistance in HCC.
WORLD JOURNAL OF GASTROENTEROLOGY
(2021)
Article
Biochemistry & Molecular Biology
Abul K. Azad, Maikel A. Farhan, Cameron R. Murray, Kunimasa Suzuki, Gary Eitzen, Nicolas Touret, Ronald B. Moore, Allan G. Murray
Summary: Angiogenesis is essential in embryonic development and tissue repair. FGD5, an endothelial Rho GTP/GDP exchange factor, has been found to regulate CXCR4-dependent signals in endothelial cells and play a role in angiogenesis. Inhibition of FGD5 activity may complement current angiogenesis inhibitor drugs.
Article
Environmental Sciences
Churaibhon Wisessaowapak, Apichaya Niyomchan, Daranee Visitnonthachai, Naphada Leelaprachakul, Piyajit Watcharasit, Jutamaad Satayavivad
Summary: Arsenic can lead to insulin and IGF-1 resistance in human neuroblastoma cells, disrupting cellular signaling and potentially affecting growth and neurite formation by inhibiting tyrosine kinase activity.
ENVIRONMENTAL TOXICOLOGY
(2023)
Article
Cell Biology
Jamal Rahmani, Alberto Montesanto, Edward Giovannucci, Hamid Zand, Meisam Barati, John J. Kopchick, Mario G. Mirisola, Vincenzo Lagani, Hiba Bawadi, Raffaele Vardavas, Alessandro Laviano, Kaare Christensen, Giuseppe Passarino, Valter D. Longo
Summary: The association between IGF-1 levels and all-cause mortality is complex, with both low and high levels being associated with increased mortality risk. This meta-analysis found that higher IGF-1 levels were not associated with increased all-cause mortality compared to low levels. However, there was a U-shaped relationship between IGF-1 levels and mortality risk, with both low and high IGF-1 levels increasing the risk compared to middle levels. A specific range of 120-160 ng/ml was associated with the lowest mortality risk.
Article
Dermatology
Xiao Feng, Yu Ji, Ce Zhang, Tingting Jin, Jingyu Li, Jincai Guo
Summary: CCL6 promotes M2 polarization, inhibits macrophage autophagy, and accelerates skin wound healing by activating the PI3-kinase/Akt signaling pathway.
EXPERIMENTAL DERMATOLOGY
(2023)
Article
Pharmacology & Pharmacy
Nyera H. El-Shafei, Mai A. Zaafan, Esraa A. Kandil, Rabab H. Sayed
Summary: Benign prostatic hyperplasia (BPH) is a non-malignant enlargement of prostate cells that causes lower urinary tract symptoms. The pathogenesis of BPH involves various factors such as androgens receptors signaling pathways, oxidative stress, apoptosis, and potential changes in IGF-1/PI3K/AKT/FOXO pathway. This study investigates the potential beneficial effects of simvastatin on testosterone-induced BPH.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Hsin-Yu Ho, Ping-Ju Chen, Yi-Ching Chuang, Yu-Sheng Lo, Chia-Chieh Lin, Ming-Ju Hsieh, Mu-Kuan Chen
Summary: Picrasidine I exerts anticancer effects in nasopharyngeal carcinoma by modulating HO-1 via the ERK and Akt signaling pathways.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Pharmacology & Pharmacy
Zhou Yu, Haoxun Wang, Guofeng You
Summary: This study investigates the regulatory effects of the hepatic hormone IGF-1 on hOAT4 in the kidney and placenta, showing that IGF-1 can increase the transport activity, protein expression, and SUMOylation of hOAT4 through the PKB signaling pathway.
BIOCHEMICAL PHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Takeshi Ijuin, Tadaomi Takenawa
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2015)
Article
Biochemistry & Molecular Biology
Takeshi Ijuin, Naoya Hatano, Tetsuya Hosooka, Tadaomi Takenawa
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2015)
Article
Biochemistry & Molecular Biology
Takeshi Ijuin, Tetsuya Hosooka, Tadaomi Takenawa
MOLECULAR AND CELLULAR BIOLOGY
(2016)
Article
Oncology
Takeshi Ijuin, Yukiko Takeuchi, Yohei Shimono, Miki Fukumoto, Emi Tokuda, Tadaomi Takenawa
Article
Biochemistry & Molecular Biology
Yoshikazu Tamori, Sanshiro Tateya, Takeshi Ijuin, Yuki Nishimoto, Shinsuke Nakajima, Wataru Ogawa
Article
Cell Biology
Takeshi Ijuin, Naoya Hatano, Tadaomi Takenawa
Article
Oncology
Emi Tokuda, Toshiki Itoh, Junya Hasegawa, Takeshi Ijuin, Yukiko Takeuchi, Yasuhiro Irino, Miki Fukumoto, Tadaomi Takenawa
Article
Biochemistry & Molecular Biology
Takeshi Ijuin, Tadaomi Takenawa
JOURNAL OF BIOLOGICAL CHEMISTRY
(2012)
Article
Biochemistry & Molecular Biology
Takeshi Ijuin, Tadaomi Takenawa
JOURNAL OF BIOLOGICAL CHEMISTRY
(2012)
Article
Biochemistry & Molecular Biology
Takeshi Ijuin, Tadaomi Takenawa
MOLECULAR AND CELLULAR BIOLOGY
(2012)
Article
Multidisciplinary Sciences
Hideaki Ando, Matsumi Hirose, Laura Gainche, Katsuhiro Kawaai, Benjamin Bonneau, Takeshi Ijuin, Toshiki Itoh, Tadaomi Takenawa, Katsuhiko Mikoshiba
Article
Multidisciplinary Sciences
Shaymaa Mohamed Mohamed Badawy, Taro Okada, Taketoshi Kajimoto, Takeshi Ijuin, Shun-ichi Nakamura
SCIENTIFIC REPORTS
(2017)
Review
Oncology
Takeshi Ijuin
SEMINARS IN CANCER BIOLOGY
(2019)
Article
Oncology
Miki Fukumoto, Takeshi Ijuin, Tadaomi Takenawa