Article
Cell & Tissue Engineering
Fengyi Liu, Xu Wang, Yuzhou Li, Mingxing Ren, Ping He, Lu Wang, Jie Xu, Sheng Yang, Ping Ji
Summary: In this study, a photocrosslinked GelMA/PAMAM-MA hydrogel was developed as a stem cell carrier for cartilage repair. The hybrid hydrogel showed improved structural and mechanical properties compared to GelMA alone, and demonstrated a sufficient ability to promote cartilage regeneration in both in vitro and in vivo experiments. This study provides new insight into the design strategy of stem cell delivery scaffolds.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Engineering, Biomedical
Man Zhang, Qian Sun, Yiling Liu, Zhiqin Chu, Leixiao Yu, Yong Hou, Heemin Kang, Qiang Wei, Weifeng Zhao, Joachim P. Spatz, Changsheng Zhao, Elisabetta A. Cavalcanti-Adam
Summary: Tunable mechanical properties of hydrogels play a key role in regulating stem cell differentiation. By modulating cellular spatial sensing of integrin-adhesive ligands on low stiffness hydrogels, the mechanosensing and osteogenic differentiation of MSCs can be promoted to levels comparable with those observed on stiffer hydrogels. This approach opens up new possibilities for designing biomaterials and tissue scaffolds for regenerative therapeutics.
Article
Biochemistry & Molecular Biology
Vadym Burchak, Fritz Koch, Leonard Siebler, Sonja Haase, Verena K. Horner, Xenia Kempter, G. Bjoern Stark, Ute Schepers, Alisa Grimm, Stefan Zimmermann, Peter Koltay, Sandra Strassburg, Gunter Finkenzeller, Filip Simunovic, Florian Lampert
Summary: This study evaluated the printability and biocompatibility of a semi-synthetic hydrogel system (GelNB/GelS) for bioprinting of adipose-tissue-derived mesenchymal stem cells (ASCs). The results showed that GelNB/GelS hydrogels supported ASCs viability, proliferation and differentiation. The mechanical properties of GelNB/GelS system influenced the differentiation fate of ASCs. The hydrogel system may have potential applications in in vivo tissue engineering.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Aida Selaru, Alexandra-Elena Mocanu-Dobranici, Elena Olaret, Raluca-Elena Ginghina, Izabela-Cristina Stancu, Marieta Costache, Sorina Dinescu
Summary: In this study, fibrous nanostructured substrates based on fish gelatin enriched with graphene oxide and magnetic nanoparticles were fabricated. The biological properties of these substrates, including cell viability, adhesion, and differentiation, were evaluated. The results showed good interaction between the materials and cells, indicating their potential for tissue engineering applications, with a concentration of 0.5% graphene oxide and magnetic nanoparticles being the most suitable option for biological testing.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Engineering, Biomedical
Ting Zou, Shan Jiang, Baicheng Yi, Qixin Chen, Boon Chin Heng, Chengfei Zhang
Summary: The study demonstrated that SCAPs induced in BDNF-GelMA with small molecule cocktail showed significant upregulation of neural markers, exhibiting morphological characteristics similar to neuronal cells, which may provide a therapeutic platform for neurological disorders.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2022)
Article
Biotechnology & Applied Microbiology
Yuhang Deng, Yiming Li, Zhuyang Chu, Chun Dai, Jianjun Ge
Summary: In this study, the combination of human umbilical cord mesenchymal stem cell-derived exosomes and GelMA was found to effectively promote endothelial repair of vein grafts and inhibit restenosis. Therefore, this biomaterial-based therapeutic approach holds promise as a treatment for venous graft restenosis.
JOURNAL OF NANOBIOTECHNOLOGY
(2023)
Article
Engineering, Biomedical
Gyeong Min Lee, Se-jeong Kim, Eun Mi Kim, Eunhyung Kim, Sangmin Lee, Eunjin Lee, Hee Ho Park, Heungsoo Shin
Summary: A composite hydrogel incorporating epigallocatechin-gallate (EGCG)-coated nanofiber particles (EF) was developed. Compared to traditional GelMA hydrogel, this composite hydrogel showed significant improvements in cell viability, intracellular oxidative stress, and cell spreading and migration ability.
ACTA BIOMATERIALIA
(2022)
Article
Biochemistry & Molecular Biology
Niloofar Asghari, Shiva Irani, Mohamad Pezeshki-Moddaress, Mojgan Zandi, Marjan Mohamadali
Summary: Tissue engineering using PVA/Gel/Cro nanofiber scaffolds was effective in promoting cell proliferation and differentiation, particularly when containing 5% crocin. The differentiation of human bone marrow-derived mesenchymal stem cells into neural-like cells on the scaffolds was confirmed by genetic and protein-level markers.
MOLECULAR BIOLOGY REPORTS
(2022)
Article
Materials Science, Biomaterials
Yuan Ma, Yilin Wang, Danni Chen, Ting Su, Qiang Chang, Wenhua Huang, Feng Lu
Summary: Current hydrogel-based scaffolds present challenges in mimicking the physical microenvironment of tissues combined with therapeutic biological cues. This study developed a 3D bioprinted gelatin-alginate hydrogel for gradient composite scaffolds that mimic the dermal stiffness microenvironment. Adipose-derived stem cells encapsulated in the scaffolds showed enhanced proliferation, migration, angiogenesis, and healing efficiency, attributed to the physical microenvironment provided by the gradient stiffness scaffold.
JOURNAL OF MATERIALS CHEMISTRY B
(2023)
Article
Multidisciplinary Sciences
Mariyan J. Jeyarajah, Gargi Jaju Bhattad, Rachel D. Kelly, Kelly J. Baines, Adam Jaremek, Fei-Hung P. Yang, Hiroaki Okae, Takahiro Arima, Vanessa Dumeaux, Stephen J. Renaud
Summary: GCM1 is a critical regulator of human placenta development, playing a key role in the differentiation and function of EVT and ST. Knockdown of GCM1 hinders TS cell differentiation and impacts their functions in both ST and EVT pathways.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
Mariyan J. Jeyarajah, Gargi Jaju Bhattad, Rachel D. Kelly, Kelly J. Baines, Adam Jaremek, Fei-Hung P. Yang, Hiroaki Okae, Takahiro Arima, Vanessa Dumeaux, Stephen J. Renaud
Summary: GCM1 is a critical regulator of both EVT and ST development, affecting cell differentiation and invasive capacity, potentially leading to obstetrical complications such as preeclampsia.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Cell & Tissue Engineering
Naruphong Phunikom, Nittaya Boonmuen, Pakpoom Kheolamai, Kanoknetr Suksen, Sirikul Manochantr, Chairat Tantrawatpan, Duangrat Tantikanlayaporn
Summary: Andrographolide (AP) has been shown to promote proliferation, osteogenic differentiation, and bone formation of placenta-derived mesenchymal stem cells. It may be an effective novel agent for improving placenta-derived MSCs and stem cell-based therapy for bone regeneration. The compound enhances Wnt/beta-catenin signaling and osteoblast-specific gene expressions, promoting bone formation and increasing osteocalcin levels.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Computer Science, Interdisciplinary Applications
Goezde Dursun, Saurabh Balkrishna Tandale, Rutwik Gulakala, Joerg Eschweiler, Mersedeh Tohidnezhad, Bernd Markert, Marcus Stoffel
Summary: The study aimed to develop a potential CNN model for recognizing differentiated stem cells by learning features directly from image data of unlabelled cells. The results showed that CNN models can classify cells accurately, and training with image enhancement and data augmentation strategies can improve classification accuracy.
COMPUTER METHODS AND PROGRAMS IN BIOMEDICINE
(2021)
Article
Cell & Tissue Engineering
Yang Zhang, Yanqi Zhong, Weifang Liu, Fanghui Zheng, Yin Zhao, Li Zou, Xiaoxia Liu
Summary: The study revealed the importance of PFKFB3-mediated glycolysis in endothelial differentiation and angiogenesis of PMSCs, showing that PMSCs have a strong potential for endothelial differentiation.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Biochemistry & Molecular Biology
Thomas Distler, Ines Lauria, Rainer Detsch, Clemens M. Sauter, Farina Bendt, Julia Kapr, Stephan Ruetten, Aldo R. Boccaccini, Ellen Fritsche
Summary: Biodegradable hydrogels composed of oxidized alginate, gelatin, and laminin promote neuronal differentiation and growth of human induced pluripotent stem cell (hiPSC) derived neurospheres in three-dimensional (3D) culture. The addition of laminin to the hydrogels enhances neuron migration from neurospheres to the bulk matrix, leading to a significant increase in the number of migrated neurons. This study highlights the importance of laminin in tailoring oxidized alginate-gelatin hydrogels for 3D neuronal cell culture applications.
Article
Materials Science, Multidisciplinary
Jing Fu, Somayya E. Taher, Rashid K. Abu Al-Rub, Tiejun Zhang, Vincent Chan, Kin Liao
Summary: A novel piezoresistive sensor based on a 3D MXene scaffold has been developed, which exhibits high compressive strength, thermal conductivity, and sensitivity, demonstrating its potential in pressure distribution measurement.
ADVANCED ENGINEERING MATERIALS
(2022)
Article
Materials Science, Coatings & Films
Chuan Li, Jang Hsing Hsieh, Chuan Ming Su, Nai-Yun Chang
Summary: Vanadium oxide thin films with various oxygen contents were deposited by sputtering and annealed to obtain different properties. The films were characterized using various techniques to determine their crystal structure, vibrational modes, electrical resistivity, and optical properties. The results show that the films exhibit thermochromic behavior within a certain range of oxygen supply.
JOURNAL OF VACUUM SCIENCE & TECHNOLOGY A
(2022)
Article
Materials Science, Multidisciplinary
Jang-Hsing Hsieh, Chuan Li, Weite Wu, Shan-Lun Liu
Summary: This study successfully reduced the friction coefficient and increased the hydrophobicity of TaN coatings by introducing carbon and fluorine. It was found that increasing the carbon and fluorine contents in the films significantly improved hemocompatibility and minimized the effects of moisture on the friction coefficient.
Article
Polymer Science
Hsiu Yu Chi, Nai Yun Chang, Chuan Li, Vincent Chan, Jang Hsin Hsieh, Ya-Hui Tsai, Tingchao Lin
Summary: In this study, a new type of mixed hydrogel nanofiber was fabricated from gelatin and polyvinyl alcohol by electrospinning. The optimal composition of gelatin and polyvinyl alcohol in nanofiber fabrication was determined through extensive characterization of various physicochemical properties of mixed polymer solutions. The resulting mixed hydrogel nanofibers exhibited favorable properties for potential applications in various fields.
Article
Materials Science, Multidisciplinary
Stephen T. Hsieh, Himanshu Mishra, Nima Bolouki, Weite Wu, Chuan Li, Jang-Hsing Hsieh
Summary: A plasma system with internal coil and sputtering carbon targets was used to deposit PP-MMA thin films. The plasma conditions and deposition rates were varied by adjusting the working pressure and Ar/MMA flow ratio. Optical emission spectroscopy and Fourier transformed infrared spectrometry were employed to study the plasma characteristics and vibrational modes of the deposited films.
Article
Materials Science, Multidisciplinary
Himanshu Mishra, Nima Bolouki, Stephen T. Hsieh, Chuan Li, Weite Wu, Jang-Hsing Hsieh
Summary: In this study, plasma generation of plasma-polymerized methyl methacrylate (PP-MMA) on the inner surface of a silicone tube was analyzed through optical emission spectroscopy (OES). A hollow cathode model was proposed to determine the occurrence of plasma discharge inside the tube, and the sheath thickness was calculated analytically to initiate polymerization processes. The electron temperature and plasma density were measured and calculated using modified Boltzmann plot and line-ratio method. It was found that plasma could be generated inside the tube if the inner diameter is greater than two times the sheath thickness. The effect of MMA monomer concentration on plasma generation and deposition was investigated. The presence of PP-MMA films on the inner surface of the tube was confirmed using attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy.
Article
Nanoscience & Nanotechnology
Mochamad Januar, Suhendro Purbo Prakoso, Chia-Wen Zhong, Horng-Chih Lin, Chuan Li, Jang-Hsing Hsieh, Kuo-Kang Liu, Kou-Chen Liu
Summary: This paper demonstrates a fully room-temperature fabrication of p-type SnOx thin films with high mobility using ion-beam-assisted deposition. The technique allows independent control of ion density and energy, optimizing the optical band gap and hole mobility of the films. The absence of annealing process prevents microcrystal formation and improves the performance of the films.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Chemistry, Physical
Baosong Li, Shaohong Luo, Shoaib Anwer, Vincent Chan, Kin Liao
Summary: Heterogeneous films assembled from 2D porous double-layered carbon nanosheets and Ti3C2Tx MXene exhibit high-performance EMI shielding, mainly due to their unique structure and material combination.
APPLIED SURFACE SCIENCE
(2022)
Review
Polymer Science
Xinyu Wang, Vincent Chan, Peter R. Corridon
Summary: Extensive and permanent damage to blood vessels necessitates innovative therapeutics such as drugs, medical devices, and cell therapies. One promising approach involves engineering bioartificial/biomimetic vessels to replace malfunctioning ones, but further research is required to effectively integrate them into patient's existing vasculature. This review assesses the design criteria, engineering factors, and innovative approaches for creating and characterizing biomimetic macro- and micro-scale vessels. The correlation between polymer properties and biological functionalities of acellular vascular segments is examined, along with emerging characterization techniques for probing tissue-engineered vascular grafts. Challenges in the clinical transformation of engineered vessels derived from polymers are identified, and future research directions are discussed.
Article
Materials Science, Multidisciplinary
Jang-Hsing Hsieh, Chuan Li, Weite Wu, Yi-Hwa Lai, Shu-Chuan Liao, Chih-Chien Hu, Yu-Han Chang
Summary: TaO0.2N0.8-Ag nanocomposite coatings were fabricated by reactive multi-target sputtering, and suitable amount of Ag doping and rapid thermal annealing treatment could further improve the hardness and crack resistance. The coatings also exhibited high antibacterial efficiency. Surface roughness was found to be the dominating factor for cell viability and attachment, and the samples with 1.5 at. % and 11.0 at. % Ag showed the best biocompatibility.
Article
Materials Science, Multidisciplinary
Nai Yun Chang, Chuan Li, Jang-Hsing Hsieh
Summary: In this study, vanadium oxynitride thin films were deposited by reactive magnetron sputtering using pure vanadium targets and a mix of N-2 and O-2 as reactive gases. The range of the process parameters for magnetron sputtering to deposit vanadium oxynitride thin films was defined. Experimental results showed that the annealed films can be oxynitrides when the oxygen flow rate is below 0.25 sccm and the ratio of oxygen/nitrogen is no more than approximately 1/10. The recommended deposition conditions to obtain vanadium oxynitride films with relatively lower resistivity and optical transmittance were O-2:N-2 = 1/20, O-2 < 0.25 sccm, and 600 degrees C annealing.
Article
Chemistry, Analytical
Dawit Bogale Alemayehu, Masahiro Todoh, Jang-Hsing Hsieh, Chuan Li, Song-Jeng Huang
Summary: To enhance the properties of pure titanium and improve the feasibility of medical implants, advancements in titanium processing technologies are necessary. This study aimed to surface modify commercially pure titanium using micro-arc oxidation (MAO) or plasma electrolytic oxidation (PEO) technologies and evaluate its mechanical strength, corrosion resistance, and cytotoxicity. The results showed that the mechanical grain refinement method and surface modification can improve the mechanical and biomechanical properties of pure titanium, with 2PassMAO demonstrating the lowest corrosion rate and high effectiveness in reducing corrosion. The study also provided valuable insights into pulp and periodontal cell behavior and material cytotoxicity.
Article
Materials Science, Composites
Rajeev Kumar, Sunny Zafar, Himanshu Pathak, Murugan Subramani, Chuan Li, Song-Jeng Huang
Summary: The integration of mesoscale modeling and macroscale experimentation has been used to predict the fatigue performance of carbon-fiber-reinforced polymer composites under cyclic loading conditions in this study. The mean field homogenization technique is implemented and Modified Gerber criteria with stress-based Tsai-Hill failure indicator are used to predict the number of fatigue cycles. Fatigue strength factor and creep rupture strength factor are evaluated experimentally and implemented in a computational approach. The effect of composite constituents, stress ratio, and loading direction are investigated against the fatigue performance of the composite.
JOURNAL OF COMPOSITES SCIENCE
(2023)
Article
Biotechnology & Applied Microbiology
Hsin-Tzu Lu, Jia-Yi Lin, Yu-Chuan Tsuei, Yung-Fu Hsu, Chung-Yi Chen, Shih-Hao Cheng, William Chu, Chuan Li, Woei-Chyn Chu
Summary: Osteoporosis-induced vertebral compression fracture (OVCF) is common in people over 50, especially menopausal women. Minimally invasive vertebroplasty (PVP) and kyphoplasty (PKP) have been successfully used in clinical treatment, but the risk of bone cement leakage is a major concern. In this study, a new aspiration technique (APV) was introduced to reduce the leakage risk, and its effectiveness was verified using ex vivo vertebral bodies. The results showed that APV significantly reduced the leakage rate to 13% compared to 53% with conventional PV, and achieved more uniform cement distribution.
BIOENGINEERING-BASEL
(2023)
