Review
Biochemistry & Molecular Biology
Marko Nedeljkovic, Diego Emiliano Sastre, Eric John Sundberg
Summary: The bacterial flagellum is a complex nanomachine that propels bacteria through liquids, playing critical roles in survival, reproduction, and pathogenicity. Additionally, flagellins have potential as adjuvants in vaccine development.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Microbiology
Yunjie Chang, Hui Xu, Md A. Motaleb, Jun Liu
Summary: Researchers have discovered a multiprotein collar complex in Lyme disease spirochetes, which exhibits remarkable structural plasticity to accommodate the highly curved membrane and generate the high torque necessary for spirochete motility.
Article
Biochemistry & Molecular Biology
Paolo Rossi, Qiong Xing, Elisabetta Bini, Athina G. Portaliou, Mary C. Clay, Eric M. Warren, Nandish K. Khanra, Anastassios Economou, Charalampos G. Kalodimos
Summary: This article investigates the interaction of late-stage flagellar chaperones FliT and FlgN with the export controller protein FliJ in Salmonella enterica. The study finds that FliT and FlgN bind cooperatively to FliJ with high affinity at specific sites. It is discovered that the binding of chaperones disrupts the coiled-coil structure of FliJ and alters its interactions with the export gate. The researchers propose that FliJ aids in the release of substrates from chaperones and plays a role in chaperone recycling during late-stage flagellar assembly.
JOURNAL OF MOLECULAR BIOLOGY
(2023)
Article
Microbiology
Marco J. Kuehn, Daniel B. Edelmann, Kai M. Thormann
Summary: This study explores how two different flagellar systems in a bacterium can work together efficiently for spreading. The interaction between the secondary lateral flagella and the main polar flagellum supports the bacterial locomotion in specific conditions.
ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Microbiology
Naoto Eguchi, Shunya Suzuki, Kenji Yokota, Shizunobu Igimi, Akinobu Kajikawa
Summary: The study shows that the two flagellins of Ligilactobacillus agilis BKN88 together form a more robust filament than either alone, indicating they are functionally complementary.
Article
Immunology
Yusuke Hoshino, Taro Sakamoto, Naoki Sudo, Masahiro Ito, Takeshi Haneda, Nobuhiko Okada, Tsuyoshi Miki
Summary: LCFA metabolism plays a significant role in the gut colonization of Salmonella enterica serovar Typhimurium by regulating the expression of fljB, which maintains proper flagellar motility.
INFECTION AND IMMUNITY
(2022)
Article
Biochemistry & Molecular Biology
Jiaxing Tan, Xing Zhang, Xiaofei Wang, Caihuang Xu, Shenghai Chang, Hangjun Wu, Ting Wang, Huihui Liang, Haichun Gao, Yan Zhou, Yongqun Zhu
Summary: This study provides detailed molecular insights into the structure, assembly, and torque transmission mechanisms of the bacterial flagellar motor.
Article
Biochemistry & Molecular Biology
Lunni Zhou, Haobin Liu, Siyu Liu, Xiaoyu Yang, Yue Dong, Yun Pan, Zhuang Xiao, Beihong Zheng, Yan Sun, Pengyu Huang, Xixi Zhang, Jin Hu, Rui Sun, Shan Feng, Yi Zhu, Mingxi Liu, Miao Gui, Jianping Wu
Summary: Using cryo-EM and AI-based modeling, the structures of mouse and human sperm DMTs were determined and an atomic model of the mouse sperm DMT was built. Analysis revealed 47 DMT-associated proteins, including 45 MIPs. The study also identified variants in 10 distinct MIPs associated with asthenozoospermia, expanding the genetic spectrum of male infertility.
Article
Biology
Jyot D. Antani, Anita X. Sumali, Tanmay P. Lele, Pushkar P. Lele
Summary: The chemotaxis network in Helicobacter pylori regulates the direction of rotation in the bacterial flagellar motor to facilitate cell migration towards favorable chemical environments. This modulation results in cells swimming faster or slower depending on the direction of flagellar rotation. Exposure to a chemo-attractant decreases the rotational bias, leading to cells swimming exclusively in the faster mode, while the absence of the chemotaxis protein CheY results in a zero bias. The relationship between reversal frequency and rotational bias is unimodal in this species.
Article
Biochemistry & Molecular Biology
Alina Guse, Manuel Halte, Svenja Huesing, Marc Erhardt
Summary: The study revealed that the type-III secretion system of bacterial flagellum has a remarkable substrate specificity during flagellar assembly, accurately secreting the required substrates at different stages, and the specificity switch is unidirectional and irreversible.
MOLECULAR MICROBIOLOGY
(2021)
Article
Microbiology
Sandra Sanchez, Caroline M. Dunn, Daniel B. Kearns
Summary: This study identifies the lytic transglycosylase CwlQ as a critical enzyme for flagellum-dependent swarming motility in Gram-negative bacteria, while the lyase essential for flagellar insertion in Gram-positive bacteria remains unknown. Even when mutated in combination with other lyases related to motility, CwlQ mutation does not affect flagellar biosynthesis in Gram-positive bacteria.
JOURNAL OF BACTERIOLOGY
(2021)
Article
Cell Biology
Tatiana Brustovetsky, Rajesh Khanna, Nickolay Brustovetsky
Summary: In Huntington's disease, hyperphosphorylation of CRMP2 and its dissociation from proteins involved in mitochondrial dynamics lead to increased mitochondrial fission and reduced motility, highlighting a potential regulatory role of CRMP2 in mitochondrial dynamics and neuronal survival in HD.
Article
Microbiology
Ping Lu, Huawei Zhang, Yuanzhu Gao, Xudong Jia, Zhe Liu, Daping Wang, Shannon Wing Ngor Au, Qinfen Zhang
Summary: Helicobacter pylori is a major risk factor for gastric diseases, and the flagellar motor is a crucial virulence factor. This study focuses on the role of FliY in flagellar motor assembly in H. pylori. The results show that the loss of FliY or its C-terminal domain disrupts the formation of key components of the flagellar motor, while complementation with the C-terminal domain restores these missing components. This study provides structural insights into the assembly of the flagellar motor in H. pylori and deepens our understanding of its pathogenicity.
Article
Microbiology
Ping Lu, Huawei Zhang, Yuanzhu Gao, Xudong Jia, Zhe Liu, Daping Wang, Shannon Wing Ngor Au, Qinfen Zhang
Summary: Research has shown that the flagellar C-ring protein FliY of Helicobacter pylori and its C-terminal domain play crucial roles in the assembly of the flagellar motor. Deletion of FliY or its C-terminal domain results in the loss of the C ring, while deletion of FliY N-terminal domain does not impact C-ring assembly and flagellar structures.
Review
Biochemistry & Molecular Biology
Fabiana San Martin, Lenka Fule, Gregorio Iraola, Alejandro Buschiazzo, Mathieu Picardeau
Summary: This article provides a comprehensive review of the function and structure of spirochetal flagella, uncovering paradigm-shifting mechanisms of bacterial motility.
TRENDS IN MICROBIOLOGY
(2023)
Article
Cell Biology
Cecil J. Gomes, Michael W. Harman, Sara M. Centuori, Charles W. Wolgemuth, Jesse D. Martinez
Article
Multidisciplinary Sciences
Alex E. Hamby, Dhruv K. Vig, Sasha Safonova, Charles W. Wolgemuth
Article
Cell Biology
Oleg A. Igoshin, Jing Chen, Jianhua Xing, Jian Liu, Timothy C. Elston, Michael Grabe, Kenneth S. Kim, Jasmine A. Nirody, Padmini Rangamani, Sean X. Sun, Hongyun Wang, Charles Wolgemuth
MOLECULAR BIOLOGY OF THE CELL
(2019)
Article
Mathematics, Applied
Tiankui Zhang, Charles W. Wolgemuth
JOURNAL OF SCIENTIFIC COMPUTING
(2020)
Article
Cell Biology
Charles W. Wolgemuth, Sean X. Sun
MOLECULAR BIOLOGY OF THE CELL
(2020)
Article
Zoology
Sriram Chandrasekaran, Nicole Danos, Uduak Z. George, Jin-Ping Han, Gerald Quon, Rolf Muller, Yinphan Tsang, Charles Wolgemuth
Summary: The biological challenges facing humanity are complex, multi-factorial, and crucial for the future of health, welfare, and the environment. Tackling these issues requires integrating diverse datasets, developing new frameworks, and promoting transparency in models and interdisciplinary collaboration.
INTEGRATIVE AND COMPARATIVE BIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Yessica K. Gomez, Andrew M. Natale, James Lincoff, Charles W. Wolgemuth, John M. Rosenberg, Michael Grabe
Summary: This study reveals consistent buckling distortions in a large membrane patch during molecular dynamics simulations using the Monte-Carlo barostat with a hard Lennard-Jones cutoff, while smaller patches show signs of reduced surface area and thickening, accompanied by compressive load on the membrane. The mismatch in handling potentials and forces in the dynamical equations versus the MC barostat is found to be responsible for these differential behaviors, suggesting a minimal compression of the membrane's linear dimensions is required for buckling. It is recommended to always use LJ force or potential-switching when employing the MC barostat to prevent undesirable membrane deformations.
JOURNAL OF COMPUTATIONAL CHEMISTRY
(2022)
Article
Computer Science, Interdisciplinary Applications
Tiankui Zhang, Charles W. Wolgemuth
Summary: The dynamics of thin, membrane-like structures are crucial in cell biology, but simulating large scale deformations remains challenging due to spatial heterogeneity. A general computational framework has been developed to simulate the dynamics of membranes and vesicles, successfully predicting equilibrium shapes of multiphase vesicles.
JOURNAL OF COMPUTATIONAL PHYSICS
(2022)
Article
Computer Science, Interdisciplinary Applications
Paul M. Ryan, Charles W. Wolgemuth
Summary: Filaments, rods, and beams are commonly found in various fields. However, there are still many unanswered questions regarding the dynamics of bending and twisting these long, thin objects. This paper presents a finite volume discretization method based on the Kirchoff equations that guarantees the correct total integral of forces and torques on filaments, rods, or beams. By coupling this discretization with resistive force theory, a stable and accurate dynamic algorithm for filament motion at low Reynolds number is developed. The utility, stability, and accuracy of this method are demonstrated through a range of sample problems.
JOURNAL OF COMPUTATIONAL PHYSICS
(2022)
Article
Physics, Fluids & Plasmas
Paul M. Ryan, Charles W. Wolgemuth
Summary: This study investigates the phase diagram and dynamic behaviors of twirling filaments based on their cross-sectional aspect ratio and rotational frequency.
PHYSICAL REVIEW FLUIDS
(2022)
Article
Mechanics
Charles W. W. Wolgemuth, Jorge I. I. Palos-Chavez
Summary: The dynamics of suspensions of particles has been extensively studied since Einstein's calculation of the leading-order correction to viscosity. In this study, we focus on the low-Reynolds-number behavior of spherical particle suspensions. By deriving a continuum-level description based on the Green's functions for flow, we correct errors in previous derivations and obtain stable equations of motion for particles and fluid. The resulting equations provide accurate predictions for sedimentation speed and viscosity corrections.
JOURNAL OF FLUID MECHANICS
(2023)
Article
Biophysics
Yizeng Li, Lijuan He, Nicolas A. P. Gonzalez, Jenna Graham, Charles Wolgemuth, Denis Wirtz, Sean X. Sun
BIOPHYSICAL JOURNAL
(2017)
Article
Biophysics
Dhruv K. Vig, Alex E. Hamby, Charles W. Wolgemuth
BIOPHYSICAL JOURNAL
(2017)
Article
Biophysics
David Argudo, Neville P. Bethel, Frank V. Marcoline, Charles W. Wolgemuth, Michael Grabe
BIOPHYSICAL JOURNAL
(2017)
Article
Biophysics
Michael W. Harman, Alex E. Hamby, Ross Boltyanskiy, Alexia A. Belperron, Linda K. Bockenstedt, Holger Kress, Eric R. Dufresne, Charles W. Wolgemuth
BIOPHYSICAL JOURNAL
(2017)