Article
Microbiology
Yu Han, Yu Li, Zhiwei Zeng, Wenjing Li, Saixiang Feng, Weisheng Cao
Summary: This study explores the resistance mechanism and activity of the antimicrobial peptide MccY against Salmonella strains. The deletion of Ton system genes resulted in resistance to MccY and mutants showed increased susceptibility to MccY under a low-iron condition. MccY may decrease the virulence of S. Typhimurium by altering multiple physiological properties.
MICROBIOLOGY SPECTRUM
(2022)
Article
Biochemistry & Molecular Biology
Qingjie Li, Lianping Wang, Jingwen Xu, Shuang Liu, Zeyu Song, Tingting Chen, Xuming Deng, Jianfeng Wang, Qianghua Lv
Summary: In this study, the compound quercitrin was found to inhibit the invasion of S. Typhimurium into host cells and reduce host cell damage. It directly inhibited the function of T3SS, providing a potential antibiotic alternative against S. Typhimurium infection.
Article
Microbiology
Seul Kim, Eunsuk Kim, Hyunjin Yoon
Summary: Salmonella alters cellular processes to improve intracellular fitness during host infection by rewiring cellular transcriptional regulation through alternative sigma factors such as rpoS. Deprivation of outer membrane protein YcfR in Salmonella Typhimurium leads to decreased intracellular survival and downregulation of SPI-2 genes, which is abolished in the absence of rpoS. RpoS-associated stress responses in Salmonella due to impaired envelope integrity may reciprocally downregulate the expression of SPI-2 genes to reduce its virulence.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Kaifeng Chen, Zeqiang Zhan, Lili Li, Jiayi Li, Zhouping Zhou, Nanwei Wang, Dage Sun, Chenggang Xu, Pengju Chen, Xiaoyun Qu, Ming Liao, Jianmin Zhang
Summary: This study investigated the role of BolA gene in Salmonella Typhimurium and found that BolA promotes biofilm formation and enhances resistance to stress and host cell invasion by regulating gene expression. Therefore, BolA gene may be a potential target for therapeutic strategies to control Salmonella Typhimurium infections.
MICROBIOLOGICAL RESEARCH
(2023)
Article
Gastroenterology & Hepatology
Meiying Yan, Yongming Zhou, Yang Cao, Zhenpeng Li, Xin Lu, Bo Pang, Shukun Wang, Biao Kan
Summary: This study discovered a new serovar of Salmonella (S. enterica subsp. II serovar 4,5,12:a:-) and demonstrated its ability to infect humans and cause clusters of cases. Whole-genome sequencing detection and surveillance of Salmonella can accurately define Salmonella classification and clonality, improve diagnosis, facilitate outbreak detection, and aid in the source tracing of salmonellosis epidemics.
Article
Food Science & Technology
Rui Dong, Xiaojie Qin, Shoukui He, Xiujuan Zhou, Yan Cui, Chunlei Shi, Yiping He, Xianming Shi
Summary: The study revealed that DsrA plays a crucial role in promoting oxidative stress resistance in S. Typhimurium by regulating the expression of antioxidant enzymes and maintaining intracellular redox balance.
Article
Gastroenterology & Hepatology
Nirmal Kumar Mohakud, Rakesh Kumar Panda, Saumya Darshana Patra, Bikash Ranjan Sahu, Mrinmoy Ghosh, Gajraj Singh Kushwaha, Namrata Misra, Mrutyunjay Suar
Summary: The study analyzed a strain of Salmonella isolated from a patient with gastroenteritis in Odisha, India, revealing highly conserved genes related to drug resistance and virulence, suggesting the potential dissemination of a novel multi-drug resistant strain with enhanced virulence.
Article
Microbiology
Jingyan Shu, Hongtao Liu, Yang Liu, Xindi Chen, Yu Yu, Qianghua Lv, Jianfeng Wang, Xuming Deng, Zhimin Guo, Jiazhang Qiu
Summary: Tannic acid (TA) has been shown to improve survival rates and alleviate cecal pathological lesions in S. Typhimurium-infected mice, while inhibiting bacterial invasion of HeLa cells. TA may exert its anti-infection effects by controlling the expression of key genes in the T3SS.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Immunology
Zecai Zhang, Siyu Liu, Jiang Huang, Yueqi Cui, Yu Liu, Yulong Zhou, Zhanbo Zhu
Summary: The study found that phloretin can alleviate intestinal damage caused by Salmonella and inhibit its translocation to other organs. Phloretin protects colonic tissue by reducing inflammation and oxidative stress levels, and prevents Salmonella from damaging the colonic epithelium barrier by regulating ZO-1 and occludin levels. Additionally, phloretin does not inhibit Salmonella growth but reduces its internalization into Caco-2 cells.
MICROBIAL PATHOGENESIS
(2021)
Article
Biotechnology & Applied Microbiology
Yezhi Fu, Nkuchia M. M'ikanatha, Jeffrey M. Lorch, David S. Blehert, Brenda Berlowski-Zier, Chris A. Whitehouse, Shaoting Li, Xiangyu Deng, Jared C. Smith, Nikki W. Shariat, Erin M. Nawrocki, Edward G. Dudley
Summary: Salmonella enterica serovar Typhimurium isolates from wild birds in the United States exhibit distinct lineages and virulence gene signatures, indicating host adaptation. These isolates are genetically different from those originating from domestic animal sources and can be attributed to wild birds with improved accuracy using genomic data and machine learning classifiers. The findings highlight the importance of wild birds as reservoirs of S. Typhimurium and provide valuable information for source attribution and epidemiological surveillance.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Microbiology
Laura Elpers, Lena Lueken, Fabio Lange, Michael Hensel
Summary: The number of outbreaks caused by gastrointestinal pathogens associated with produce is increasing. The mechanisms involved in the colonization, persistence, and transmission of these pathogens on fresh produce are poorly understood. This study focused on the adhesive factors of S. enterica serovar Typhimurium on butterhead lettuce (Lactuca sativa) and identified several factors that contribute to its adhesion to lettuce leaves.
MICROBIOLOGY SPECTRUM
(2023)
Article
Chemistry, Analytical
Han Du, Xu Zhang, Mingru Yao, Qingli Yang, Wei Wu
Summary: This study successfully constructed aptamer-guided luminous microspheres using carbon dots fluorescence, which can efficiently recognize and detect Salmonella enterica serovar Typhimurium. The method shows high sensitivity and has broad application prospects.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Microbiology
Yu-feng Qiu, Reshma B. Nambiar, Xue-bin Xu, Shun-tai Weng, Hang Pan, Kui-cheng Zheng, Min Yue
Summary: Non-typhoidal Salmonella (NTS) is a common cause of gastroenteritis, with the invasive infection caused by an infrequently reported serovar Telelkebir strain FJ001 in China carrying antimicrobial-resistant and typhoidtoxin genes. Genomic investigation revealed a high relatedness among global S. Telelkebir isolates, suggesting potential rapid transmissions through international travels. This study highlights the importance of surveillance and understanding the threat posed by the unique combination of virulent and antimicrobial-resistant determinants in NTS infections.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
Xue Fu, Lan Feng, Linghan Kong, Chun Li, Xiaodong Zhao, Huade Li, Pengfei Cui, Wenjun Yan, Yaru Zhai, Lan Zhang, Hao Li, Hongning Wang, Xin Yang
Summary: The study investigated the prevalence of Salmonella Derby in yak farms in the Qinghai-Tibetan Plateau, demonstrating its high pathogenicity to mice. Findings suggest that Salmonella Derby excreted by diarrheic yaks could be a source of contamination for other yaks and the environment.
Article
Agriculture, Dairy & Animal Science
H. Xue, D. Wang, B. M. Hargis, G. Tellez-Isaias
Summary: The reduction in antibiotic use in poultry has created a need for natural solutions to control pathogens like Salmonella. NeutraPath, a natural feed additive, was found to inhibit the growth of Salmonella in vitro and reduce its colonization in broilers in vivo. It also downregulated the expression of virulence genes and preserved intestinal barrier integrity.
Editorial Material
Immunology
Ferric C. Fang, Robert T. Schooley
CLINICAL INFECTIOUS DISEASES
(2022)
Article
Microbiology
Alessandro Moriano, Diego O. Serra, Amparo Hoard, Sabrina Montana, Jose Degrossi, Robert A. Bonomo, Krisztina M. Papp-Wallace, Maria Soledad Ramirez
Summary: Polymicrobial lung infections in individuals with Cystic Fibrosis are complex and can be caused by opportunistic pathogens like S. aureus, P. aeruginosa and Burkholderia cepacia complex. Interaction studies between S. aureus and Bcc reveal potential impact on the severity and outcomes of infections in CF patients.
CURRENT MICROBIOLOGY
(2021)
Article
Chemistry, Medicinal
Michiyoshi Nukaga, Michael J. Yoon, Magdalena A. Taracilia, Tyuji Hoshino, Scott A. Becka, Elise T. Zeiser, Joseph R. Johnson, Krisztina M. Papp-Wallace
Summary: The Burkholderia cepacia complex (Bcc) poses a serious health threat to individuals with cystic fibrosis or compromised immune systems due to its high level of antibiotic resistance, notably through the PenA-like beta-lactamases. This study evaluated different beta-lactamase inhibitors against PenA1 to restore susceptibility to antibiotics in whole cell based assays, revealing avibactam as the most effective inhibitor. Specific amino acids, such as S130, R220, and K234, were found to play important roles in inactivation by avibactam, indicating potential targets for future drug development.
ACS INFECTIOUS DISEASES
(2021)
Article
Immunology
Joyce E. Karlinsey, Angela M. Fung, Norah Johnston, Howard Goldfine, Stephen J. Libby, Ferric C. Fang
Summary: Cyclopropanation of membrane lipids enhances stress resistance and virulence in Salmonella bacteria.
INFECTION AND IMMUNITY
(2022)
Editorial Material
Microbiology
Krisztina M. Papp-Wallace, Jean-Denis Docquier, Frederic Kerff, Pablo Power
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Gastroenterology & Hepatology
Helit Cohen, Boaz Adani, Emiliano Cohen, Bar Piscon, Shalhevet Azriel, Prerak Desai, Heike Bahre, Michael McClelland, Galia Rahav, Ohad Gal-Mor
Summary: This study investigates the changes in the DksA regulon during Salmonella evolution, showing that DksA plays a crucial role in the growth, gene expression, and invasion of Salmonella. It regulates multiple metabolic pathways and virulence genes. The study also reveals the assimilation of horizontally acquired virulence genes under the DksA regulon, providing insights into the evolution of virulence gene regulation in Salmonella species.
Article
Microbiology
T. A. Alsenani, S. L. Viviani, V Kumar, M. A. Taracila, C. R. Bethel, M. D. Barnes, K. M. Papp-Wallace, R. K. Shields, M. H. Nguyen, C. J. Clancy, R. A. Bonomo, F. van den Akker
Summary: The structural study of KPC-2 D179 variants revealed destabilization of the Omega loop, contributing to ceftazidime-avibactam resistance.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Microbiology
Magdalena A. Taracila, Christopher R. Bethel, Andrea M. Hujer, Krisztina M. Papp-Wallace, Melissa D. Barnes, Joseph D. Rutter, Jamie VanPelt, Ben A. Shurina, Focco van den Akker, Cornelius J. Clancy, M. Hong Nguyen, Shaoji Cheng, Ryan K. Shields, Richard C. Page, Robert A. Bonomo
Summary: This study investigates the susceptibility and resistance mechanism of D179Y variant of KPC-2 and -3 to carbapenems and ceftazidime and ceftazidime-avibactam, and it reveals that conformational changes and overall disorder caused by D179Y substitution contribute to the unexpected phenotype.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Microbiology
Philip Hinchliffe, Catherine L. Tooke, Christopher R. Bethel, Benlian Wang, Christopher Arthur, Kate J. Heesom, Stuart Shapiro, Daniela M. Schlatzer, Krisztina M. Papp-Wallace, Robert A. Bonomo, James Spencer
Summary: This study reveals the mechanism of action of two β-lactamase inhibitors, enmetazobactam and tazobactam, on CTX-M-15 β-lactamase in Escherichia coli, including the formation of protein cross-link and epimerization of amino acids.
Article
Microbiology
Melina B. Cian, Joshua A. Mettlach, Aaron E. Zahn, Nicole P. Giordano, Keaton E. Minor, Michael McClelland, Zachary D. Dalebroux
Summary: The three cardiolipin (CL) synthase genes of Salmonella enterica serovar Typhimurium work cooperatively to promote intracellular survival and inflammasome activation in macrophages, but do not affect inflammatory cytokine production downstream of Tlr4 or virulence in C57BL/6J mice.
MICROBIOLOGY SPECTRUM
(2022)
Article
Infectious Diseases
Scott A. Becka, Elise T. Zeiser, John J. LiPuma, Krisztina M. Papp-Wallace
Summary: This study evaluated the antimicrobial activity of Tebipenem against multiple strains of Enterobacterales pathogens. The results showed that all strains of Burkholderia cepacia complex (Bcc) and Burkholderia gladioli tested were resistant to Tebipenem. Further experiments revealed that the resistance may be related to the inducible beta-lactamases PenA and AmpC produced by these strains.
Article
Biochemistry & Molecular Biology
Clemens M. Gehrer, Alexander Hoffmann, Richard Hilbe, Philipp Grubwieser, Anna-Maria Mitterstiller, Heribert Talasz, Ferric C. Fang, Esther G. Meyron-Holtz, Sarah H. Atkinson, Gunter Weiss, Manfred Nairz
Summary: The sequestration of iron in case of infection is a strategy of host defense, but the function of ferritin in bacterial infections is not fully understood. Iron bound to ferritin is less available to Salmonella, but still enhances bacterial growth significantly. Superoxide dismutase may weaken bacterial iron uptake from ferritin by siderophore-independent mechanisms.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Vinay Kumar, Pavneet Randhawa, Robert Bilodeau, Dan Mercola, Michael McClelland, Anshu Agrawal, James Nguyen, Patricia Castro, Michael M. Ittmann, Farah Rahmatpanah
Summary: In prostate cancer, the tumor microenvironment plays a crucial role in the development and progression of the disease. This study utilized Digital Spatial Profiling multiplex technology to assess the expression of proteins and RNA transcripts in challenging samples. The results revealed differences in protein and RNA expression among different cell types and validated the protein expression in a larger cohort of prostate cancer patients. Furthermore, closely examining the tumor microenvironment provides valuable data that can improve prognostication and treatment techniques.
Article
Microbiology
Jennifer E. Kurasz, Madison C. Crawford, Steffen Porwollik, Oliver Gregory, Katerina R. Tadlock, Eve C. Balding, Emily E. Weinert, Michael McClelland, Anna C. Karls
Summary: The adaptation of Salmonella enterica serovar Typhimurium to stress conditions involves the expression of genes regulated by RpoN and activated by RtcR. The activation of RtcR is dependent on a RecA-controlled SOS response pathway and the presence of a specific region within the prophage Gifsy-1. This study highlights the role of prophages in controlling the activity of regulatory proteins and altering bacterial physiological functions.
JOURNAL OF BACTERIOLOGY
(2023)
Article
Microbiology
Arturo Casadevall, Ferric Fang
Summary: Decisions involving cooperation or competition are common in science. This article examines three common scenarios in biomedical sciences – establishing priority, sharing reagents, and selecting a journal for publication – using the prisoner's dilemma as a framework. In each situation, cooperation is found to be the optimal strategy for scientists and for the advancement of science.
