Article
Multidisciplinary Sciences
Maximilian Merz, Almuth Maria Anni Merz, Jie Wang, Lei Wei, Qiang Hu, Nicholas Hutson, Cherie Rondeau, Kimberly Celotto, Ahmed Belal, Ronald Alberico, AnneMarie W. Block, Hemn Mohammadpour, Paul K. Wallace, Joseph Tario, Jesse Luce, Sean T. Glenn, Prashant Singh, Megan M. Herr, Theresa Hahn, Mehmet Samur, Nikhil Munshi, Song Liu, Philip L. McCarthy, Jens Hillengass
Summary: This study characterizes osteolytic lesions (OL) in multiple myeloma patients using single-cell RNA sequencing (scRNA-seq), revealing specifically regulated genes in OL compared to random bone marrow samples, as well as their response to induction therapy.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Xifeng Chen, Pengcheng Liu, Le Mei, Xiaoling He, Long Chen, Hui Liu, Shurong Shen, Zhandong Ji, Xixi Zheng, Yuchen Zhang, Zhenyu Gao, Dali Zeng, Qian Qian, Bojun Ma
Summary: The study presents a novel executor R gene, Xa7, which confers extremely durable, broad-spectrum, and heat-tolerant resistance to bacterial blight (BB) in rice. The expression of Xa7 is induced by incompatible Xoo strains, with faster and stronger induction under high temperatures, triggering a hypersensitive response in plants. The research findings on the interaction between Xa7 and Xoo strains will greatly aid in understanding the molecular mechanisms of Xa7-mediated resistance and its potential use in breeding programs.
PLANT COMMUNICATIONS
(2021)
Editorial Material
Chemistry, Physical
Vasily Bulatov, Wei Cai
Summary: The in situ electron microscopy study provides important insights into the enhanced dislocation mobility in iron and contributes to the ongoing debate on hydrogen embrittlement mechanisms.
Review
Oncology
L. Nicolas Gonzalez Castro, Itay Tirosh, Mario L. Suva
Summary: This article discusses the recent advances in single-cell expression profiling in tumor research and emphasizes the powerful insights gained from these studies in key aspects of tumor biology, particularly in the context of intratumor heterogeneity.
Editorial Material
Hematology
Helene Schoemans, Per Ljungman
Summary: The article investigates the antibody response in patients who received cellular therapy and COVID-19 vaccination.
Editorial Material
Nanoscience & Nanotechnology
Nadim Darwish
Summary: Transport measurements through a single molecule reveal the mechanism of the catalytic Suzuki-Miyaura cross-coupling reaction.
NATURE NANOTECHNOLOGY
(2021)
Review
Cell Biology
Kaustubh Wagh, Diana A. Stavreva, Arpita Upadhyaya, Gordon L. Hager
Summary: This review summarizes the dynamic nature of transcription factor interactions with chromatin in the cell nucleus. Beginning with a historical overview, the key factors that govern transcription factor dynamics are highlighted, as well as the impact of transcription factor dynamics on downstream transcriptional bursting. Finally, open challenges and emerging technologies in the field of transcriptional regulation are discussed.
ANNUAL REVIEW OF CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Engineering, Environmental
Jiayu Zhang, Chao Yang, Jiahui Hu, Yiting Zhang, Yuezheng Lai, Hongri Gong, Fangliang Guo, Xiaoyan Li, Lin Ye, Bing Li
Summary: This study discovered a novel antibiotic resistance gene capO and elucidated its role in chloramphenicol resistance, indicating its potential mobility and accumulation in wastewater treatment, which provides important insights for tackling the antibiotic resistance crisis.
Editorial Material
Oncology
Ivan Ballesteros, Daniela Cerezo-Wallis, Andres Hidalgo
Summary: This article provides a comprehensive analysis of lung cancer patients at the single-cell level, revealing extreme immune diversity and defining neutrophil populations associated with treatment outcomes.
Editorial Material
Multidisciplinary Sciences
Amber Dance
Summary: Methods that combine imaging with sequencing are revealing the organizational principles of the genome and its impact on cell fates.
Article
Biochemistry & Molecular Biology
Shibani Mohapatra, Saumya Ranjan Barik, Prasanta K. Dash, Devidutta Lenka, Kartika Chandra Pradhan, K. R. Reshmi Raj, Shakti Prakash Mohanty, Mihir Ranjan Mohanty, Ambika Sahoo, Binod Kumar Jena, Alok Kumar Panda, Debabrata Panigrahi, Sushanta Kumar Dash, Jitendriya Meher, Chitta Ranjan Sahoo, Arup Kumar Mukherjee, Lipi Das, Lambodar Behera, Sharat Kumar Pradhan
Summary: Ranidhan, a popular rice variety in Odisha state, India, is prone to damage from flash floods and susceptible to bacterial blight disease. In order to enhance its resistance and submergence tolerance, researchers used marker-assisted backcross breeding to introduce BB resistance genes and submergence tolerance genes into Ranidhan. Through selection and backcrossing, a new rice variety with high resistance and similar qualities to Ranidhan was developed.
Article
Engineering, Environmental
Pinggui Cai, Qian Chen, Wenran Du, Shanqing Yang, Jiarui Li, Hetong Cai, Xiaohui Zhao, Weiling Sun, Nan Xu, Jiawen Wang
Summary: Little is known about the dynamics of metal resistance genes (MRGs) and antibiotic resistance genes (ARGs) under different metal(loid) contamination levels. This study systematically investigated MRGs and ARGs in three zones with different contamination levels across an abandoned sewage reservoir. The findings showed that diverse MRGs and ARGs were detected in the highly contaminated zone, while specific bacteria in the moderately contaminated zone promoted the vertical gene transfer of MRGs and ARGs.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Editorial Material
Biochemistry & Molecular Biology
Aila Akosua Kattner
Summary: In this issue of Biomedical Journal, various advancements in medical research are covered, including COVID-19 prevention, HIV treatment, drug resistance in liver cancer, and therapeutic approaches for breast cancer. The journal showcases a wide range of cutting-edge achievements in the field of medicine.
BIOMEDICAL JOURNAL
(2021)
Editorial Material
Biochemistry & Molecular Biology
Folakemi T. T. Odedina, Rafaela Alves Pacheco, Marcia C. C. Castro
Summary: Tailored community engagement, led or co-led by the community, can establish trust and ensure health equity in underserved communities.
Review
Marine & Freshwater Biology
Beiyu Hu, Peng Xu, Liang Ma, Dongwei Chen, Jian Wang, Xin Dai, Li Huang, Wenbin Du
Summary: Microbes play a crucial role in influencing the Earth's environment, yet remain poorly understood due to limited capacity. The emerging technology of droplet microfluidics offers promising solutions for microbial studies, enabling high-throughput single-cell cultivation, screening, and sequencing. This review provides an overview of droplet microfluidics techniques for microbial research, focusing on critical steps in the microfluidic system and recent progress in microbiological applications.
MARINE LIFE SCIENCE & TECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Matthew J. Prior, Jebasingh Selvanayagam, Jung-Gun Kim, Monika Tomar, Martin Jonikas, Mary Beth Mudgett, Sjef Smeekens, Johannes Hanson, Wolf B. Frommer
Summary: The pathogen Pseudomonas can promote the expression of nutrient transporters, such as SWEETs and UmamiT amino acid transporters, by regulating the host transcription factor bZIP11, affecting the pathogen titer in leaves.
MOLECULAR PLANT-MICROBE INTERACTIONS
(2021)
Article
Plant Sciences
Reika Isoda, Akira Yoshinari, Yuuma Ishikawa, Mayuri Sadoine, Rudiger Simon, Wolf B. Frommer, Masayoshi Nakamura
Summary: Plant hormones play crucial roles in growth, development, and environmental adaptation. Understanding hormone signaling networks is important for monitoring hormone production, transportation, perception, and distribution. Various tools, such as transcriptional reporters and degradation sensors, are available for quantifying and visualizing hormone dynamics.
Article
Chemistry, Multidisciplinary
Mayuri Sadoine, Mira Reger, Ka Man Wong, Wolf B. Frommer
Summary: By improving the dynamic range of the FLIPsuc sensor and expanding the detection range from micro- to millimolar sucrose concentrations, researchers have developed a series of sucrose sensors that may enable investigation of sucrose transporter candidates and comprehensive in vivo analyses of sucrose concentration in plants. The new series of sensors, which can also detect trehalose in animal cells, will likely be suitable for investigating trehalose transport and monitoring trehalose steady-state levels in vivo.
Review
Plant Sciences
Noriyoshi Yagi, Akira Yoshinari, Ryu J. Iwatate, Reika Isoda, Wolf B. Frommer, Masayoshi Nakamura
Summary: Fluorescent probes are essential tools for visualizing cellular structures and molecular dynamics in living cells, with a wide variety of small-molecule dyes synthesized for labeling purposes. Self-labeling technologies allow these probes to be attached to cellular domains or specific proteins, and are now being increasingly utilized in plant research.
PLANT AND CELL PHYSIOLOGY
(2021)
Article
Plant Sciences
Vanessa Castro-Rodriguez, Thomas J. Kleist, Nicoline M. Gappel, Fatiha Atanjaoui, Sakiko Okumoto, Mackenzie Machado, Tom Denyer, Marja C. P. Timmermans, Wolf B. Frommer, Michael M. Wudick
Summary: Plants use electrical and chemical signals for systemic communication, with herbivory triggering glutamate accumulation and calcium waves to report damage and initiate defense. By expressing fluorescent glutamate sensors in Arabidopsis, researchers found that displaying the sensors externally impacted plant growth and development. The study also revealed that sequestering glutamate at the cell surface disrupts meristematic cell supply and localized signaling, affecting root growth.
Article
Multidisciplinary Sciences
Jihyun Park, Taylor M. Chavez, Jordan A. Guistwhite, Sojeong Gwon, Wolf B. Frommer, Lily S. Cheung
Summary: SWEETs are a type of transporter found in various organisms, including plants, which facilitate the release of sugars from cells. Researchers have developed genetically encoded biosensors to measure the activity of SWEETs and have successfully created a mass action kinetics model to describe their function.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Plant Sciences
Manuel Miras, Mathieu Pottier, T. Moritz Schladt, J. Obinna Ejike, Laura Redzich, Wolf B. Frommer, Ji-Yun Kim
Summary: This article reviews the current knowledge about plasmodesmata (PD), unique cell-cell connections in plants, and discusses the protein composition, structure, transport mechanism, and regulation of PD. It emphasizes the importance of PD in photoassimilate transport and carbohydrate allocation, and highlights the need for new approaches and technologies to advance the understanding of PD biology.
JOURNAL OF PLANT PHYSIOLOGY
(2022)
Article
Plant Sciences
Lin-Bo Wu, Joon-Seob Eom, Reika Isoda, Chenhao Li, Si Nian Char, Dangping Luo, Van Schepler-Luu, Masayoshi Nakamura, Bing Yang, Wolf B. Frommer
Summary: SWEETs play important roles in intercellular sugar transport. OsSWEET11b has roles in male fertility and bacterial blight susceptibility in rice, possibly depending on sucrose transport activity. Induction of OsSWEET11b by TALe increases the virulence of Xoo. The identification of OsSWEET11b is relevant for protecting rice against new Xoo strains targeting this gene.
Article
Plant Sciences
Marcel Buchholzer, Wolf B. Frommer
Article
Biochemistry & Molecular Biology
Thomas J. Kleist, I. Winnie Lin, Sophia Xu, Grigory Maksaev, Mayuri Sadoine, Elizabeth S. Haswell, Wolf B. Frommer, Michael M. Wudick
Summary: The interaction between physical forces and membrane proteins is crucial for environmental sensing and acclimation. We developed genetically encoded osmolality sensors by fusing fluorescent protein spectral variants to mechanosensitive ion channels. These sensors reported changes in osmolality through the emission ratio of fluorescent protein domains.
Article
Multidisciplinary Sciences
Reika Isoda, Zoltan Palmai, Akira Yoshinari, Li-Qing Chen, Florence Tama, Wolf B. Frommer, Masayoshi Nakamura
Summary: SWEET sucrose transporters play important roles in the allocation of sucrose in plants and have been shown to mediate transport of the plant growth regulator gibberellin (GA). The study reveals a functional connection between sucrose and GA and demonstrates that sucrose is the physiologically relevant substrate. These findings are important for understanding the role of SWEETs in pathogen susceptibility and male fertility.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Plant Sciences
Sven Gombos, Manuel Miras, Vicky Howe, Lin Xi, Mathieu Pottier, Neda S. Kazemein Jasemi, Moritz Schladt, J. Obinna Ejike, Ulla Neumann, Sebastian Haensch, Franziska Kuttig, Zhaoxia Zhang, Marcel Dickmanns, Peng Xu, Thorsten Stefan, Wolfgang Baumeister, Wolf B. Frommer, Ruediger Simon, Waltraud X. Schulze
Summary: Plasmodesmata (PD) facilitate movement of molecules between plant cells, but the regulation of this movement is still not well understood. This study developed a PD scoring approach to define a high-confidence PD proteome (HC300) and identified previously unlocalized proteins in PD. The results provide insights into the diversification of large protein families and strengthen the reliability of HC300 for further exploration of the structure, function, and evolution of PD.
Article
Plant Sciences
Ji-Yun Kim, Eliza P- Loo, Tin Yau Pang, Martin Lercher, Wolf B. Frommer, Michael M. Wudick
Summary: Sucrose, hexoses, and raffinose are key players in plant metabolism, with SUTs, SWEETs, and UmamiTs facilitating the translocation of sucrose and amino acids across different cell types. These transporters also play crucial roles in important processes like seed filling, nectar secretion, and pollen nutrition, while pathogens target SWEETs to exploit the plant's vulnerabilities. This research provides insights into the intercellular transport of key metabolites and highlights the potential for future studies in plant-microbe interactions and transporter functions.
Article
Plant Sciences
Mayuri Sadoine, Yuuma Ishikawa, Thomas J. Kleist, Michael M. Wudick, Masayoshi Nakamura, Guido Grossmann, Wolf B. Frommer, Cheng-Hsun Ho
Summary: Understanding the spatial dynamics of small molecules and the activities of enzymes, transporters, and other proteins in multicellular organisms is crucial for studying signaling and metabolic processes. Genetically encoded sensors, such as fluorescent proteins, have been developed for monitoring a wide range of small molecules and biophysical processes. Advances in imaging technologies have allowed researchers to implement sensor technologies in intact plants, enabling quantification and visualization of ion and metabolite distribution and dynamics.
Article
Biochemical Research Methods
Nora R. Zoellner, Margaret Bezrutczyk, Reinout Laureyns, Hilde Nelissen, Ruediger Simon, Wolf B. Frommer
Summary: This study presents an optimized protocol for RNA in situ hybridization in maize leaf tissue, which can also be adapted for other plant tissues. By generating multiple unique antisense probes and hybridizing them to tissue sections, the experimental efficiency can be improved.
