Article
Plant Sciences
Jianbo Li, Yinguang Bao, Ran Han, Xiaolu Wang, Wenjing Xu, Guangrong Li, Zujun Yang, Xiaojun Zhang, Xin Li, Aifeng Liu, Haosheng Li, Jianjun Liu, Peng Zhang, Cheng Liu
Summary: This study characterized wheat-Thinopyrum intermedium introgression lines using various methods and identified Thinopyrum chromosomes carrying stem rust resistance genes.
Article
Agronomy
Shanying Zhu, Haonan Du, Fuyu Su, Jin Wang, Qingfeng Meng, Tianlei Liu, Rui Guo, Zhaozhao Chen, Huanhuan Li, Wenxuan Liu, Pengtao Ma, Huagang He
Summary: In this study, two wheat-rye derivatives, JS016 and JS110, were developed and identified as T6BS.6RL and T6BS.6BL6RL translocation lines, respectively. These lines were found to possess novel powdery mildew resistance genes and exhibited better agronomic traits compared to the previously reported Pm20 translocation line.
Article
Plant Sciences
Xiaojun Zhang, Jianbo Li, Yudi Ge, Haixia Guan, Guangrong Li, Shuwei Zhang, Xiaolu Wang, Xin Li, Zhijian Chang, Peng Zhang, Juqing Jia, Cheng Liu
Summary: This study developed a wheat-Th. intermedium substitution line CH51, which exhibited high levels of resistance to leaf rust and stripe rust diseases in the field. In addition, CH51 lacked a pair of wheat chromosome 6B.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Yizhuo Liu, Weiwei Song, Anning Song, Chunfei Wu, Jiarui Ding, Xiaoning Yu, Jia Song, Miaomiao Liu, Xinyuan Yang, Changtong Jiang, Haibin Zhao, Weifu Song, Dongjun Liu, Xuefeng Yang, Qingjie Song, Xinling Li, Lei Cui, Hongjie Li, Yanming Zhang
Summary: The perennial wheatgrass lines 19HSC-Q and 20HSC-Z, developed through hybridization, exhibited good cold resistance and agronomic traits, which is significant for breeding cold-resistant perennial wheatgrass.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Plant Sciences
Fei Qi, Shuang Liang, Piyi Xing, Yinguang Bao, Richard R. -C. Wang, Xingfeng Li
Summary: This study used molecular cytogenetics to investigate the karyotype composition of intermediate wheatgrass and found variability in the karyotypes of different accessions. The researchers concluded that the chromosomes of intermediate wheatgrass mainly originated from Dasypyrum breviaristatum and the genus Pseudoroegneria.
Article
Plant Sciences
Mingzhu Li, Yuanyuan Yuan, Fei Ni, Xingfeng Li, Honggang Wang, Yinguang Bao
Summary: In this study, two wheat-Th. ponticum introgression lines were developed, exhibiting excellent resistance to powdery mildew and desirable agronomic traits. Genetic analyses confirmed the introgression nature of these lines through specific markers and chromosomal structural variations.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Guangrong Li, Qiheng Chen, Wenxi Jiang, Ahui Zhang, Ennian Yang, Zujun Yang
Summary: Using multi-color non-denaturing fluorescence in situ hybridization (ND-FISH), a novel disease-resistant wheat-Th. intermedium double substitution line X479 was identified. Through sequential ND-FISH with multiple probes, 15 types of wheat-Thinopyrum translocation chromosomes were developed and characterized. The wheat-4J(S)L translocation lines showed high levels of resistance to stripe rust prevalent races.
Article
Plant Sciences
Zhihui Yu, Hongjin Wang, Ennian Yang, Guangrong Li, Zujun Yang
Summary: This study successfully identified each linkage group of the Th. intermedium chromosomes using non-denaturing fluorescent in situ hybridization and oligo-FISH painting techniques. The karyotype of Th. intermedium with individual chromosome recognition was established. Chromosome constitutions of wheat-Th. intermedium derived lines were characterized, revealing distinct intergenomic chromosome rearrangements. These precisely defined derived lines may contribute to the study of chromosome evolution, chromatin introgression, and wheat breeding programs.
Article
Plant Sciences
Mingzhu Li, Yanzhen Wang, Xiaojuan Liu, Xingfeng Li, Honggang Wang, Yinguang Bao
Summary: SN19647 is a novel wheat substitution line resistant to powdery mildew and leaf rust, characterized by chromosomal substitution events from Thinopyrum ponticum and structural variation on wheat chromosomes.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Jianhua Wang, Guan-Zhu Han
Summary: Research has shown that co-option of retrotransposon gag genes is common during the deep evolution of eukaryotes, involving a wide range of organisms with a majority being Ty3/Gypsy retrotransposons. These co-opted gag genes may perform diverse cellular functions in host cells, with some of them potentially under positive selection.
MOLECULAR BIOLOGY AND EVOLUTION
(2021)
Article
Plant Sciences
Jianbo Li, Zhihui Yu, Ahui Zhang, Yan Yin, Lingrong Tang, Guangrong Li, Peng Zhang, Imtiaz Ahmed Khan, Ian Dundas, Zujun Yang
Summary: A physical map of Thinopyrum intermedium chromosome 7J was constructed using translocation mapping, and a new seedling purple coleoptile gene was mapped to the bin of FL 0.35-0.63 of 7JS. Through multicolor fluorescence in situ hybridization, PCR-based landmark unique gene (PLUG), and intron targeting (IT) markers analysis, a physical map of the 7J chromosome was constructed, consisting of eight chromosomal bins with 89 markers, locating the purple coleoptile gene. The development of a new chromosome 7J-specific EST-PCR marker from the chromosomal region corresponding to the purple coleoptile gene has facilitated fine mapping and studying the function of the purple coleoptile gene in anthocyanin biosynthesis.
Article
Biochemistry & Molecular Biology
Inpyo Hong, Jin-Young Park, Yoo-Hun Noh, Su-Hee Jeon, Jeong-Won Paik, Jung-Seok Lee, Seong-Ho Choi, Jae-Kook Cha
Summary: The study demonstrated that TEES-10(R) significantly increased PDL cell viability in vitro and suppressed the expression of inflammatory mediators. In the clinical trial, oral administration of TEES-10(R) showed significant improvement in GI and BOP at 4 weeks, reducing the number of patients diagnosed with gingivitis.
Article
Plant Sciences
Anna Kalinka, Marta Starczak, Daniel Gackowski, Edyta Stepien, Magdalena Achrem
Summary: 5-Hydroxymethylcytosine (5hmC) is found in the genomic DNA of rye species, and its quantity and chromosomal distribution suggest that it may play a role in regulating the rye genome.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Plant Sciences
Paul Gruner, Thomas Miedaner
Summary: The study found that the genetic variation of perennial rye is mainly influenced by specific loci on the chromosomes rather than the presence of chromosomal multivalents. Through QTL mapping, key genetic loci affecting perenniality and fertility were identified.
Article
Biochemistry & Molecular Biology
Samuel Simoni, Clarissa Clemente, Gabriele Usai, Alberto Vangelisti, Lucia Natali, Silvia Tavarini, Luciana G. Angelini, Andrea Cavallini, Flavia Mascagni, Tommaso Giordani
Summary: The translation discusses the importance of Stevia rebaudiana as a crop and its genetic characteristics. The study focuses on the isolation and characterization of long-terminal repeat retrotransposons (LTR-REs) and the analysis of genetic variability in different accessions using the Inter-Retrotransposon Amplified Polymorphism (IRAP) protocol.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Caiyun Liu, Wei Guo, Qiaofeng Zhang, Bisheng Fu, Zujun Yang, Sivakumar Sukumaran, Jin Cai, Ying Liu, Wenling Zhai, Xiaoyou Wu, Jizhong Wu
Summary: Genetic resistance to sharp eyespot in wheat was studied using a recombinant inbred line population. Stable QTL were identified on chromosomes 2B and 7D, as well as novel QTL on 1D, 6D, and 7A, providing potential targets for improving sharp eyespot resistance in wheat breeding programs through marker-assisted selection.
Article
Plant Sciences
Guangrong Li, Tao Zhang, Zhihui Yu, Hongjin Wang, Ennian Yang, Zujun Yang
Summary: The chromosome-specific painting technique developed in this research, using oligo probes, enables efficient high-throughput karyotyping of barley and wheat chromosomes, as well as other Triticeae species. This technique facilitates the study of inter-species chromosome homologous relationships and non-homologous chromosomal rearrangements in Triticeae species and wheat-alien species derivatives.
Article
Plant Sciences
Jianbo Li, Zhihui Yu, Ahui Zhang, Yan Yin, Lingrong Tang, Guangrong Li, Peng Zhang, Imtiaz Ahmed Khan, Ian Dundas, Zujun Yang
Summary: A physical map of Thinopyrum intermedium chromosome 7J was constructed using translocation mapping, and a new seedling purple coleoptile gene was mapped to the bin of FL 0.35-0.63 of 7JS. Through multicolor fluorescence in situ hybridization, PCR-based landmark unique gene (PLUG), and intron targeting (IT) markers analysis, a physical map of the 7J chromosome was constructed, consisting of eight chromosomal bins with 89 markers, locating the purple coleoptile gene. The development of a new chromosome 7J-specific EST-PCR marker from the chromosomal region corresponding to the purple coleoptile gene has facilitated fine mapping and studying the function of the purple coleoptile gene in anthocyanin biosynthesis.
Article
Biotechnology & Applied Microbiology
Zhihui Yu, Hongjin Wang, Wenxi Jiang, Chengzhi Jiang, Weiguang Yuan, Guangrong Li, Zujun Yang
Summary: The study established the standard karyotype of D. breviaristatum chromosomes through ND-FISH technique, using oligonucleotide probes from wheat - D. breviaristatum partial amphiploid and newly identified wheat - D. breviaristatum translocation and addition lines. The differences between D. breviaristatum and D. villosum chromosomes revealed by ND-FISH will aid in understanding evolutionary divergence of repetitive sequences within the genus Dasypyrum, and may broaden the gene pool of wheat breeding.
Article
Plant Sciences
Wenxi Jiang, Chengzhi Jiang, Weiguang Yuan, Meijun Zhang, Zijie Fang, Yang Li, Guangrong Li, Juqing Jia, Zujun Yang
Summary: A universal karyotyping nomenclature system based on oligo-FISH has been established for precise chromosome identification and comparative evolutionary studies in Avena species. This new system provides a powerful tool for describing genetic diversity, chromosomal rearrangements, and evolutionary relationships among Avena species through comparative cytogenetic and genomic approaches.
Article
Plant Sciences
Xiaolu Wang, Zhihui Yu, Hongjin Wang, Jianbo Li, Ran Han, Wenjing Xu, Guangrong Li, Jun Guo, Yan Zi, Faji Li, Dungong Cheng, Aifeng Liu, Haosheng Li, Zujun Yang, Jianjun Liu, Cheng Liu
Summary: Aegilops sharonensis, a wild relative of wheat, contains diverse disease and insect resistance genes, which can be used as a valuable gene source for wheat improvement. Molecular cytogenetic marker analysis and disease resistance investigations have identified the potential of A. sharonensis as a new resistance source for wheat breeding.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Genetics & Heredity
Shan Duan, Chunge Cui, Liang Chen, Zujun Yang, Yin-Gang Hu
Summary: This study identified and mapped the Rht14 dwarf gene in durum wheat, and found that it regulates the expression of GA2oxA9 through DNA methylation. This provides insights for cloning Rht14 and further investigating its regulatory mechanism.
FUNCTIONAL & INTEGRATIVE GENOMICS
(2022)
Article
Plant Sciences
Manyu Yang, Zujun Yang, Wuyun Yang, Ennian Yang
Summary: Our study used non-denaturing fluorescence in situ hybridization (ND-FISH) to analyze the genetic diversity among wheat lines derived from the International Maize and Wheat Improvement Center and cultivated wheat and landraces from the Chinese wheat core germplasm. We identified 137 polymorphic FISH patterns and detected 12 rearrangements, including 7 new translocations, providing cytological information for the rational utilization of wheat germplasm resources.
Article
Agronomy
Chengzhi Jiang, Wenxi Jiang, Min Liu, Hongjin Wang, Ennian Yang, Zujun Yang, Guangrong Li
Summary: The construction of the 28-chromosome karyotype of Dasypyrum breviaristatum was successfully achieved using ND-FISH and Oligo-FISH protocols. High chromosome alteration rate was observed in the progeny populations containing the 6V(b)S.2V(b)L translocation chromosomes, suggesting the presence of a gametocidal-like gene. Specific gliadin coding genes were localized on sub-telomeric regions of 6V(b)S. The positive effect of 6V(b)S on agronomic and quality characters was demonstrated. These new translocation lines will serve as valuable germplasm for breeding purposes.
Article
Plant Sciences
Zhihui Yu, Hongjin Wang, Ennian Yang, Guangrong Li, Zujun Yang
Summary: This study successfully identified each linkage group of the Th. intermedium chromosomes using non-denaturing fluorescent in situ hybridization and oligo-FISH painting techniques. The karyotype of Th. intermedium with individual chromosome recognition was established. Chromosome constitutions of wheat-Th. intermedium derived lines were characterized, revealing distinct intergenomic chromosome rearrangements. These precisely defined derived lines may contribute to the study of chromosome evolution, chromatin introgression, and wheat breeding programs.
Article
Plant Sciences
Shengfu Zhong, Bin Li, Wei Chen, Lili Wang, Ju Guan, Qiang Wang, Zujun Yang, Hao Yang, Xianshu Wang, Xiaojiao Yu, Peng Fu, Hongchang Liu, Chen Chen, Feiquan Tan, Tianheng Ren, Jinliang Shen, Peigao Luo
Summary: The environmental adaptability of eudicots is the most reasonable explanation for why they compose the largest clade of modern plants. The genome of Akebia trifoliata experienced two whole-genome duplication (WGD) events, with the older event likely occurring at the early stage of eudicots.
Article
Plant Sciences
Wei Zhang, Zongxiang Tang, Jie Luo, Guangrong Li, Zujun Yang, Manyu Yang, Ennian Yang, Shulan Fu
Summary: This study identified the clustering regions of two important tandem repeats on wheat chromosome 5B by analyzing the wheat genome sequence. Additionally, the sequence characteristics of the translocation breakpoint of the translocation T7BS.7BL-5BS were discovered. These findings have significant implications for future research and improvement of the wheat genome.
Article
Plant Sciences
Jiajia Zhao, Xingwei Zheng, Ling Qiao, Chenkang Yang, Bangbang Wu, Ziming He, Yuqing Tang, Guangrong Li, Zujun Yang, Jun Zheng, Zengjun Qi
Summary: This study analyzed the chromosome variations in 543 wheat accessions using FISH technology and identified 139 SCVs. The distribution of SCVs varied between ecological regions and different wheat varieties. SCVs were used as markers to distinguish landraces and modern cultivars. GWAS analysis identified 29 SCVs associated with 12 phenotypic traits, suggesting their potential value. This study highlights the significant effects of SCVs in wheat breeding and provides an efficient method for mining favored SCVs in wheat and other crops.
Article
Plant Sciences
Guangrong Li, Qiheng Chen, Wenxi Jiang, Ahui Zhang, Ennian Yang, Zujun Yang
Summary: Using multi-color non-denaturing fluorescence in situ hybridization (ND-FISH), a novel disease-resistant wheat-Th. intermedium double substitution line X479 was identified. Through sequential ND-FISH with multiple probes, 15 types of wheat-Thinopyrum translocation chromosomes were developed and characterized. The wheat-4J(S)L translocation lines showed high levels of resistance to stripe rust prevalent races.
Article
Plant Sciences
Chengzhi Jiang, Xiaodan Liu, Zujun Yang, Guangrong Li
Summary: In this study, the researchers established the 42-chromosome karyotype of Elymus dahuricus using non-denaturing fluorescent in situ hybridization (ND-FISH) and Oligo-FISH painting methods. They also discovered a new type of intergenomic rearrangement between 2H and 5Y. Furthermore, the comparison of centromeric tandem repeats and immunostaining results suggested that centromere-associated sequences in H subgenomes were continuously changing during the process of polyploidization.
