Review
Microbiology
Manav Jain, Gideon Stitt, Luke Son, Elena Y. Enioutina
Summary: Antibiotic resistance is a major global health problem, particularly in treating MRSA and VRE infections. Probiotics and antimicrobial peptides are promising approaches that can inhibit pathogen growth and enhance the host immune system, potentially providing new strategies against antibiotic-resistant bacteria.
Article
Infectious Diseases
Jian Peng, Biswajit Mishra, Rajamohammed Khader, LewisOscar Felix, Eleftherios Mylonakis
Summary: In this study, a smaller antimicrobial peptide C18 derived from cecropin 4 showed high antibacterial activity against various bacteria and yeasts, with a remarkable effect on drug-resistant Staphylococcus aureus. C18 also exhibited the capability to eliminate persister cells, reduce biofilm adherence, interact with DNA, and down-regulate virulence factor genes. Moreover, C18 showed promising results in a Galleria mellonella model, highlighting its potential as a therapeutic candidate for severe MRSA infections.
Review
Biochemistry & Molecular Biology
Isabella Hernandez-Aristizabal, Ivan Dario Ocampo-Ibanez
Summary: The emergence of bacteria resistant to conventional antibiotics, especially VRSA and VISA strains, poses a serious threat to global health. Developing new antimicrobial alternatives, such as antimicrobial peptides (AMPs), is urgently needed as a promising therapeutic strategy to combat resistant strains. Evaluating natural, artificial, and synthetic AMPs against VRSA and VISA strains shows great potential for clinical application.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Microbiology
Bingqian Yuan, Xiaoyu Lu, Min Yang, Qiyi He, Zhuocen Cha, Yaqun Fang, Yan Yang, Lei Xu, Jingting Yan, Ren Lai, Aili Wang, Xiaodong Yu, Zilei Duan
Summary: Staphylococcus aureus (S. aureus) is a common pathogenic bacterium with increasing drug resistance. The antimicrobial peptide GW18 shows excellent activity against various strains of S. aureus and significantly reduces infection in animal models.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Melissa J. Karau, Suzannah M. Schmidt-Malan, Scott A. Cunningham, Jayawant N. Mandrekar, Bobbi S. Pritt, Tiffany R. Keepers, Alisa W. Serio, Surya Chitra, Robin Patel
Summary: Omadacycline, vancomycin, and rifampin, as well as rifampin combination therapies, were evaluated in an experimental rat model of MRSA osteomyelitis. The results showed that rifampin combination therapy was effective in reducing MRSA recovery without developing resistance. The combination of omadacycline and rifampin yielded negative MRSA detection, suggesting its potential as a treatment for osteomyelitis.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Microbiology
Monika Kumaraswamy, Kamilla Wiull, Bishnu Joshi, George Sakoulas, Armin Kousha, Gustav Vaaje-Kolstad, Mona Johannessen, Kristin Hegstad, Victor Nizet, Fatemeh Askarian
Summary: This study revealed that MRSA-derived MVs are capable of reducing the pathogen's susceptibility to VAN, thereby increasing its resistance to treatment, particularly in interactions with human neutrophils. Additionally, the elevated expression of proteins associated with antibiotic resistance in MVs may further impact MRSA's resistance.
Article
Microbiology
Isabel Guthridge, Simon Smith, Matthew Law, Enzo Binotto, Josh Hanson
Summary: Lincosamides, particularly when used as a predominant therapy, show potential efficacy in the treatment of MRSA bacteremia, especially in young, clinically stable patients with few comorbidities. Patients receiving lincosamide-predominant therapy had lower in-hospital mortality and a lower incidence of renal complications compared to those receiving an alternative regimen. Prospective studies are needed to further define the optimal role of lincosamides in the treatment of MRSA bacteremia.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Infectious Diseases
Cheng-En Tsai, Chia-Jui Yang, Yu-Chung Chuang, Jann-Tay Wang, Wang-Huei Sheng, Yee-Chun Chen, Shan-Chwen Chang
Summary: This study aimed to determine the synergistic effects of combining CPT with DAP, VAN, or LNZ against different MRSA strains. The results showed that the CPT-DAP combination exhibited significant synergistic bactericidal effects, while the CPT-VAN and CPT-LNZ combinations did not show synergistic activity. Therefore, DAP may be a better choice for combination therapy with CPT in the treatment of MRSA infections.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Public, Environmental & Occupational Health
Ahosanul H. Shahid, K. H. M. Nazmul Hussain Nazir, Mohamed E. El Zowalaty, Ajran Kabir, Shahjahan A. Sarker, Mahbubul P. Siddique, Hossam M. Ashour
Summary: The study investigated the presence of Staphylococcus aureus in food processing environments in Mymensingh city, Bangladesh, revealing a certain percentage of isolates resistant to antibiotics. Some isolates were found to be resistant to methicillin and vancomycin, posing a threat to public health.
Article
Infectious Diseases
Jin Liang, Yuanfang Hu, Mingxia Fu, Na Li, Fengxia Wang, Xiaojun Yu, Bing Ji
Summary: The study investigated the molecular epidemiology of Staphylococcus aureus (S. aureus) in patients from the Yellow River Delta region and examined antibiotic resistance profiles in different genetic backgrounds. Antibiotic susceptibility testing, spa typing, and agr typing were conducted on all 204 strains. The results identified 39 MRSA strains and 22 hVISA isolates. The majority of MRSA isolates belonged to agrI and were associated with type IV SCCmec. The most common genotypes among MRSA population were ST59-t437-agrI-IVa and ST72-t2431-agrI-IVF. The study highlights the genetic diversity and drug resistance patterns among MSSA strains exhibiting phenotypic hVISA.
INFECTION AND DRUG RESISTANCE
(2023)
Article
Infectious Diseases
Felix Jaumaux, Kenny Petit, Anandi Martin, Hector Rodriguez-Villalobos, Marjorie Vermeersch, David Perez-Morga, Philippe Gabant
Summary: The emergence of antibiotic-resistant S. aureus is a major concern, and the discovery of new antimicrobial compounds is necessary. Targeting the interactions between commensal bacteria and pathogens in the skin microbiome offers a promising approach. We discovered two bacteriocins, cerein 7B and cerein B4080, that selectively inhibit S. aureus without affecting commensal bacterium S. epidermidis. Exposure to these bacteriocins resulted in mutations in the walK/R two-component system of S. aureus, leading to decreased sensitivity to vancomycin. Preserving commensal bacteria with selective compounds could limit resistance and competition, reducing chronic infections and the spread of antibiotic resistance.
Article
Microbiology
Robby Markwart, Niklas Willrich, Tim Eckmanns, Guido Werner, Olaniyi Ayobami
Summary: The study found that resistance to linezolid and daptomycin in VREF and MRSA between 2014 and 2018 was relatively low, indicating the continued effectiveness of these last-resort antibiotics in Europe. However, efforts to monitor and contain antimicrobial resistance should be strengthened at regional and national levels.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Rong Huang, Guang-Qing Cai, Jian Li, Xi-Sheng Li, Hai-Ting Liu, Xue-Ling Shang, Jian-Dang Zhou, Xin-Min Nie, Rong Gui
Summary: A novel nano-drug delivery system, PLT@Ag-MOF-Vanc, was constructed for the treatment of MRSA infections, showing good antibacterial activity, biocompatibility, and targeting capabilities. It killed MRSA through multiple approaches and demonstrated superior anti-infective effects in a mouse MRSA pneumonia model compared to free vancomycin.
JOURNAL OF NANOBIOTECHNOLOGY
(2021)
Article
Pharmacology & Pharmacy
Jina Kim, Young-Won Chin
Summary: The prevalence of antimicrobial-resistant bacteria poses a global challenge. Methicillin-resistant Staphylococcus aureus (MRSA), a leading cause of infections, forms biofilms on medical devices, making them more resistant to antibiotics. A study investigated the use of Raman spectroscopy to monitor MRSA biofilm formation and found that Eugenol, when applied within the first 5 hours of biofilm formation, showed promise in breaking down the biofilm. The study also demonstrated the efficacy of Raman spectroscopy coupled with principal component analysis (PCA) in identifying planktonic bacteria from biofilm conglomerates.
Article
Microbiology
Siheng Wang, Chang Cai, Yingbo Shen, Chengtao Sun, Qingxin Shi, Ningjun Wu, Shufang Zheng, Jiao Qian, Rong Zhang, Hongwei Zhou
Summary: Contezolid exhibits potent activity against MRSA and VRE strains. It shows similar or slightly better antimicrobial activity compared to linezolid. Contezolid may have potential benefits for treating infections caused by MRSA and VRE pathogens.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Rosa Morra, Jayendra Shankar, Christopher J. Robinson, Samantha Halliwell, Lisa Butler, Mathew Upton, Sam Hay, Jason Micklefield, Neil Dixon
NUCLEIC ACIDS RESEARCH
(2016)
Article
Microbiology
Brian M. Forde, Minh-Duy Phan, Jayde A. Gawthorne, Melinda M. Ashcroft, Mitchell Stanton-Cook, Sohinee Sarkar, Kate M. Peters, Kok-Gan Chan, Teik Min Chong, Wai-Fong Yin, Mathew Upton, Mark A. Schembri, Scott A. Beatson
Article
Infectious Diseases
Samantha Halliwell, Peter Warn, Abdul Sattar, Jeremy P. Derrick, Mathew Upton
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2017)
Article
Environmental Sciences
John Moat, Athanasios Rizoulis, Graeme Fox, Mathew Upton
JOURNAL OF WATER AND HEALTH
(2016)
Article
Biochemistry & Molecular Biology
Sohinee Sarkar, Leah W. Roberts, Minh-Duy Phan, Lendl Tan, Alvin W. Lo, Kate M. Peters, David L. Paterson, Mathew Upton, Glen C. Ulett, Scott A. Beatson, Makrina Totsika, Mark A. Schembri
MOLECULAR MICROBIOLOGY
(2016)
Article
Endocrinology & Metabolism
J. William Allwood, Haitham AlRabiah, Elon Correa, Andrew Vaughan, Yun Xu, Mathew Upton, Royston Goodacre
Article
Multidisciplinary Sciences
Minh Duy Phan, Brian M. Forde, Kate M. Peters, Sohinee Sarkar, Steven Hancock, Mitchell Stanton-Cook, Nouri L. Ben Zakour, Mathew Upton, Scott A. Beatson, Mark A. Schembri
Article
Microbiology
Robert William O'Hara, Peter J. Jenks, Matthew Emery, Mathew Upton
JOURNAL OF MEDICAL MICROBIOLOGY
(2019)
Article
Microbiology
Abdu Aldarhami, Arif Felek, Vikram Sharma, Mathew Upton
JOURNAL OF MEDICAL MICROBIOLOGY
(2020)
Letter
Infectious Diseases
Sean D. W. Comber, Mathew Upton, Shaun Lewin, Neil Powell, Thomas H. Hutchinson
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2020)
Article
Multidisciplinary Sciences
Katharine Hammond, Helen Lewis, Samantha Halliwell, Florie Desriac, Brunello Nardone, Jascindra Ravi, Bart W. Hoogenboom, Mathew Upton, Jeremy P. Derrick, Maxim G. Ryadnov
Article
Microbiology
James Butler, Sean D. Kelly, Katie J. Muddiman, Alexandros Besinis, Mathew Upton
Summary: This study reports the characterization of a strain of C. pauculus isolated from a hospital sink trap and assesses its potential risk to human health. The findings show that the strain is multidrug-resistant and capable of biofilm formation.
JOURNAL OF MEDICAL MICROBIOLOGY
(2022)
Article
Microbiology
Matthew J. Koch, Poppy J. Hesketh-Best, Gary Smerdon, Philip J. Warburton, Kerry Howell, Mathew Upton
Summary: Through culture-based microbial analysis of deep-sea sponges, this study discovered novel antimicrobial candidates and demonstrated antimicrobial activity, showcasing the potential for screening novel antimicrobial compounds.
Review
Infectious Diseases
Neil Powell, Jennie Stephens, Declan Kohl, Rhys Owens, Shadia Ahmed, Crispin Musicha, Mathew Upton, Bridie Kent, Sarah Tonkin-Crine, Jonathan Sandoe
Summary: This systematic review examined the effectiveness and safety of nonallergist health care worker delivery of penicillin allergy delabeling. The results showed that delabeling by nonallergists is effective and safe, with different testing methods leading to varying proportions of patients being delabeled.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2023)
Article
Microbiology
Madhura Castelino, Stephen Eyre, John Moat, Graeme Fox, Paul Martin, Pauline Ho, Mathew Upton, Anne Barton