Article
Multidisciplinary Sciences
Roger Espinosa-Saez, Sara M. Robledo, Tatiana Pineda, Javier Murillo, Cesar Zuniga, Osvaldo Yanez, Plinio Cantero-Lopez, Alex Saez-Vega, Camilo Guzman-Teran
Summary: In this study, six analogs of 2-arylquinoline were synthesized and evaluated for their antiplasmodial and leishmanicidal activity. The compounds showed moderate to high activity, making them potential safe treatment for Leishmania.
SCIENTIFIC REPORTS
(2023)
Article
Immunology
Poonam Keshav, Deepak Kumar Goyal, Sukhbir Kaur
Summary: This study focused on evaluating the antiparasitic activity and immunomodulatory effects of ellagic acid (EA) against L. donovani. The in vitro study showed that EA had parasiticidal activity and halted cell cycle progression. In vivo experiments demonstrated that EA reduced parasite burden and increased specific T cell populations, enhancing the immune response. EA was found to be safe and non-toxic in cell lines and mice.
MEDICAL MICROBIOLOGY AND IMMUNOLOGY
(2023)
Article
Immunology
Maria Adelaida Gomez, Ashton Trey Belew, Adriana Navas, Mariana Rosales-Chilama, Julieth Murillo, Laura A. L. Dillon, Theresa A. Alexander, Alvaro Martinez-Valencia, Najib M. El-Sayed
Summary: This study investigates the early host determinants of healing and non-healing responses in human cutaneous leishmaniasis caused by Leishmania (Viannia) panamensis. Transcriptomic profiling of peripheral blood mononuclear cells from CL patients showed that the parasite modulates adaptive immune cell functions early on, impacting immune responses beyond innate and phagocytic functions.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Food Science & Technology
Tatyane Goncalves Hayasaki, Thais Rosa Marques Santos, Artur Christian Garcia Silva, Marize Campos Valadares
Summary: Acute inhalation toxicity testing using animal models is limited in predicting toxicity on the respiratory system. Non-animal models, including in chemico, in vitro, ex vivo, and in silico approaches, have emerged as alternatives for preclinical assessment of respiratory toxicity of chemicals. This study characterized and evaluated a new ex vivo bovine bronchial model for assessing pulmonary toxicity, demonstrating its potential as a low-cost and reproducible tool.
FOOD AND CHEMICAL TOXICOLOGY
(2022)
Article
Multidisciplinary Sciences
Manabu Abe, Ena Nakatsukasa, Rie Natsume, Shun Hamada, Kenji Sakimura, Ayako M. Watabe, Toshihisa Ohtsuka
Summary: CRISPR/Cas-based genome editing has improved genetic modification technology. In this study, AAV donors and CRISPR/Cas9 components were simultaneously electroporated into embryos to generate knock-in animals, successfully producing knock-in rats carrying a 3.0 kb gene cassette. These findings suggest that this technique is an efficient high-throughput strategy for producing genetically modified rodents and may be applicable to other animal species.
SCIENTIFIC REPORTS
(2023)
Article
Immunology
Weilin Zeng, Hui Zhao, Wei Zhao, Qi Yang, Xinxin Li, Xiaosong Li, Mengxi Duan, Xun Wang, Cuiying Li, Zheng Xiang, Xi Chen, Liwang Cui, Zhaoqing Yang
Summary: Drug resistance in Plasmodium vivax presents a challenge to malaria elimination, with studies indicating decreased sensitivity in some regions. Research on P. vivax isolates from the China-Myanmar border found potential resistance to certain antimalarial drugs, highlighting the need for continued surveillance. Genetic variations in resistance-related genes were observed, with specific mutations associated with reduced drug sensitivity.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Engineering, Biomedical
Matthew L. Bedell, Ziwen Wang, Katie J. Hogan, Angelica L. Torres, Hannah A. Pearce, Letitia K. Chim, K. Jane Grande-Allen, Antonios G. Mikos
Summary: Extrusion bioprinted constructs with different fiber architectures were fabricated to study their effects on encapsulated human mesenchymal stem cells' tissue-specific matrix deposition and integration. The gradient fiber (G-Fib) architecture showed increased chondral integration over time, while the segmented fiber (S-Fib) architecture was associated with significant mineralization. Both architectures demonstrated similar cartilage integration as re-inserted tissue controls. The study highlights the importance of considering spatial architecture in the engineering of multi-phasic tissue environments.
ACTA BIOMATERIALIA
(2023)
Article
Microbiology
Martin Okitwi, Stephen Orena, Katairo Thomas, Patrick K. Tumwebaze, Oswald Byaruhanga, Samuel L. Nsobya, Melissa D. Conrad, Brett R. Bayles, Philip J. Rosenthal, Roland A. Cooper
Summary: In this study, susceptibilities of Plasmodium falciparum isolates from Uganda were measured, both on the day of collection and after storage. The findings indicate that short-term storage before drug susceptibility determination is feasible.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Oncology
Noora Nykanen, Rami Makela, Antti Arjonen, Ville Harma, Laura Lewandowski, Eileen Snowden, Rainer Blaesius, Ismo Jantunen, Teijo Kuopio, Juha Kononen, Juha K. Rantala
Summary: Ex vivo drug screening serves as a functional diagnostic method for therapy efficacy modeling on patient-derived tumor cells. A case study using integrated multiomics ex vivo drug screening approach demonstrated efficacy assessment in a rare metastatic squamous cell carcinoma of the parotid gland, informing treatment strategy effectively. Targeted treatments based on ex vivo measured drug efficacy, validated with histopathology and genomic profiling, achieved durable clinical responses in metastatic squamous cell carcinoma of the salivary glands.
FRONTIERS IN ONCOLOGY
(2021)
Article
Immunology
Rafaella R. Costa, Joao A. Oliveira-da-Silva, Thiago A. R. Reis, Grasiele S. V. Tavares, Debora V. C. Mendonca, Camila S. Freitas, Daniela P. Lage, Vivian T. Martins, Luciana M. R. Antinarelli, Amanda S. Machado, Raquel S. Bandeira, Fernanda Ludolf, Thais T. O. Santos, Rory C. F. Brito, Maria V. Humbert, Daniel Menezes-Souza, Mariana C. Duarte, Miguel A. Chavez-Fumagalli, Bruno M. Roatt, Elaine S. Coimbra, Eduardo A. F. Coelho
Summary: Acarbose shows specific antileishmanial activity against Leishmania infantum, with low toxicity to mammalian cells. In vivo experiments using ACA/Mic in BALB/c mice infected with L. infantum demonstrate superior therapeutic effects compared to Miltefosine or free ACA treatments.
MEDICAL MICROBIOLOGY AND IMMUNOLOGY
(2021)
Article
Virology
Jing-Wei Zhang, Han Wang, Jing Liu, Le Ma, Rong-Hong Hua, Zhi-Gao Bu
Summary: The study developed an efficient high-throughput screening assay for ZIKV inhibitors and validated several compounds with therapeutic potential for inhibiting ZIKV infection.
Article
Oncology
Mariaserena Giliberto, Deepak B. Thimiri Govinda Raj, Andrea Cremaschi, Sigrid S. Skanland, Alexandra Gade, Geir E. Tjonnfjord, Fredrik Schjesvold, Ludvig A. Munthe, Kjetil Tasken
Summary: The management of multiple myeloma (MM) is challenging, and personalized testing tools are required to determine drug sensitivities. In this study, a drug testing pipeline was established to examine ex vivo drug responses in MM cells from 44 patients. Variability in drug responses and synergistic effects of certain drug combinations were observed.
MOLECULAR ONCOLOGY
(2022)
Article
Oncology
Dina Dorrigiv, Pierre-Alexandre Goyette, Amelie St-Georges-Robillard, Anne-Marie Mes-Masson, Thomas Gervais
Summary: A major challenge in cancer treatment is predicting patients' responses to drugs. 3D tumor models such as spheroids and ex vivo tumor explants are appropriate preclinical models, but they have limitations in terms of longevity and throughput. To address this, a computer-controlled drug screening platform is presented to enable multiplexed delivery of biochemical reagents to 3D tumor models. The platform allows testing of multiple treatment conditions on over a hundred tumor models and is compatible with clinical histopathology practice.
Article
Engineering, Biomedical
Michael Hu, Xin Yi Lei, Jon D. Larson, Melissa McAlonis, Kyle Ford, Daniella McDonald, Krystal Mach, Jessica M. Rusert, Robert J. Wechsler-Reya, Prashant Mali
Summary: This study integrates CRISPR-Cas-based genome engineering and biofabrication technologies to enable genetic screens in physiologically relevant ex vivo cancer tissue models. The results demonstrate that perfusable tissue constructs can be used for large-scale CRISPR screens and emulate clinically actionable gene knockout scenarios.
Article
Veterinary Sciences
Longfei Zhang, Hongbing Xie, Hongjuan Wang, Huanzhong Ding, Gaiping Zhang, Jianhe Hu
Summary: Cefquinome exhibited time-dependent antibacterial activity against Actinobacillus pleuropneumoniae, with the maximum kill rate observed in the 0-9 hour period in broth. Pharmacokinetic/pharmacodynamic parameters were analyzed in serum and tissue cage fluid, showing values that suggest potential for optimizing dosage regimens for treating APP infection.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Immunology
Pragya Misra, Rati Tandon, Trayambak Basak, Shantanu Sengupta, Anuradha Dube
PARASITE IMMUNOLOGY
(2020)
Article
Immunology
Srija Moulik, Joyshree Karmakar, Sumit Joshi, Anuradha Dube, Chitra Mandal, Mitali Chatterjee
Summary: The mRNA expression of IL-4- and IL-10-driven markers was enhanced in both experimental models of visceral leishmaniasis, demonstrating an increased expression of these markers in the presence of Leishmania donovani infection. However, only in the hamster model, a positive correlation between IL-10-driven markers and parasite load was observed in splenic tissues.
PARASITE IMMUNOLOGY
(2021)
Review
Chemistry, Multidisciplinary
Simranjeet Singh, Vijay Kumar, Dhriti Kapoor, Daljeet Singh Dhanjal, Deepika Bhatia, Sadaf Jan, Nasib Singh, Romina Romero, Praveen C. Ramamurthy, Joginder Singh
Summary: COVID-19, caused by SARS-CoV-2, has a major impact on public health and economy. Wastewater surveillance is a cost-effective method for early detection. Techniques for detection include RNA concentration and various amplification methods, while disinfectants like UV and chlorine are used to control viral spread.
ENVIRONMENTAL CHEMISTRY LETTERS
(2021)
Article
Biotechnology & Applied Microbiology
Sneha Ratnapriya, Keerti, Narendra Kumar Yadav, Anuradha Dube, Amogh Anant Sahasrabuddhe
Summary: The study focused on generating a recombinant chimeric antigen using two immunodominant Leishmania (L.) donovani antigens, with the aim of developing an effective multivalent antigen vaccine against lethal leishmaniasis. The resulting chimeric protein showed high immunogenicity and immunotherapeutic efficacy when combined with a Th1 stimulating adjuvant BCG, inhibiting splenic parasitic multiplication by 70% and boosting a Th1 dominant immune response in hamsters.
BIOMED RESEARCH INTERNATIONAL
(2021)
Article
Immunology
Ferrin Antony, Chetan Pundkar, Maninder Sandey, Anil K. Jaiswal, Amarjit Mishra, Ashok Kumar, Rudragouda Channappanavar, Amol Suryawanshi
Summary: HSV-1 infection of the cornea leads to herpetic stromal keratitis, causing severe inflammation and vision impairment. Exogenous rIFN-lambda treatment shows potential therapeutic efficacy in controlling inflammation and vision impairment induced by HSV-1 infection.
JOURNAL OF IMMUNOLOGY
(2021)
Article
Physiology
Anil Kumar Jaiswal, Jyoti Yadav, Sangeet Makhija, Maninder Sandey, Amol Suryawanshi, Amit Kumar Mitra, Amarjit Mishra
Summary: The study found that aged mice exhibit increased airway hyperresponsiveness, mixed granulomatous airway inflammation, and changes in immune response, suggesting that the development of airway inflammation may be age-related. Additionally, the age-dependent decline in SPLUNC1 levels may be involved in developing steroid-resistant airway inflammation and asthma heterogeneity.
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
(2022)
Article
Immunology
Anil Kumar Jaiswal, Jyoti Yadav, Sangeet Makhija, Suman Mazumder, Amit Kumar Mitra, Amol Suryawanshi, Maninder Sandey, Amarjit Mishra
Summary: The study revealed a direct regulatory role of the Irg1/itaconate pathway in dendritic cells for the development of airway inflammation, suggesting a possible therapeutic target in modulating allergic asthma.
MUCOSAL IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Jonathan Marable, Damien Ruiz, Anil K. Jaiswal, Ritankar Bhattacharya, Robert Pantazes, Payal Agarwal, Amol S. Suryawanshi, Deepa Bedi, Amarjit Mishra, Bruce F. Smith, Maninder Sandey
Summary: The study developed chimeric heavy chain antibodies against CTLA4 for canine cancer treatment, using nanobodies and recombinant antibodies to specifically bind to canine CTLA4, offering a novel therapeutic approach.
SCIENTIFIC REPORTS
(2021)
Editorial Material
Oncology
Karim Iskander, Marilene Paquet, Cory Brayton, Anil K. Jaiswal
Article
Respiratory System
Anil Kumar Jaiswal, Amarjit Mishra
Summary: This study found that ADAMTS7 plays a pivotal role in house dust mite-induced airway inflammation and Th2 immune response. By regulating dendritic cell function, it reduces allergic sensitization and Th2 immune response.
Article
Microbiology
Achhada Ujalkaur Avatsingh, Shilpa Sharma, Shilippreet Kour, Yukta Arora, Sheetal Sharma, Divya Joshi, Prem Prashant Chaudhary, Kahkashan Perveen, Mohab Amin Kamal, Nasib Singh
Summary: Antibiotic resistance in bacteria poses a serious threat to public health worldwide. Aquatic environments, including wastewater used for irrigation, contribute to the dissemination of antibiotic-resistant bacteria and genes, impacting human health and environmental sustainability. This study examines the prevalence of antibiotic resistance in Gram-negative bacteria isolated from irrigation-purpose wastewater in Himachal Pradesh, India. The findings highlight the presence of antibiotic-resistant and ESBL-positive bacterial isolates, emphasizing the need for ongoing monitoring and precautionary measures to manage wastewater and control environmental pollution.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biodiversity Conservation
Anuja Sharma, Shilpa Sharma, Shilippreet Kour, Achhada Ujalkaur Avatsingh, Kahkashan Perveen, Jamilah A. Alsulami, Nasib Singh
Summary: The prevalence of gastrointestinal parasites in small and large ruminants in northern India's agro-climatic region is high, especially during the rainy season. The study emphasizes the need for preventive and control strategies for effective animal health management.
Article
Oncology
Nilesh Mani, Sushil K. Aggarwal, Ishan Kumar, Abhijit Mandal, Garima Jaiswal, Rakesh Ranjan, Anil K. Jaiswal, Neha Gupta, Ankita Singh, Ankur Mourya, Lalit M. Aggarwal, Sunil Choudhary
Summary: A comparison was made between simultaneous integrated boost (SIB) and sequential boost (SEQ) in the treatment of locally advanced head and neck cancer (HNC). The study found comparable overall survival (OS) and disease-free survival (DFS) outcomes between the two treatment arms, but SIB was associated with higher acute toxicities compared to SEQ.
JOURNAL OF CANCER RESEARCH AND THERAPEUTICS
(2022)
Article
Chemistry, Medicinal
Tanuja Mishra, Debabrata Sircar, Harcharan Singh Dhaliwal, Nasib Singh
NATURAL PRODUCTS JOURNAL
(2020)
Article
Plant Sciences
Tanvir Kaur, Ajar Nath Yadav, Sushma Sharma, Nasib Singh
ARCHIVES OF PHYTOPATHOLOGY AND PLANT PROTECTION
(2020)