NW-G01, a novel cyclic hexapeptide antibiotic, produced by Streptomyces alboflavus 313: II. Structural elucidation

NW-G01 is a novel cyclic hexapeptide antibiotic produced by Streptomyces alboflavus 313. Its relative structure was established by HR-ESI-MS, IR, 1D and 2D NMR techniques, the absolute structure was determined using a combination of single-crystal X-ray diffraction and Marfey's method finally. The antibiotic consists of L-valine, (3 S)- and (3 R)-piperazic acids, N-methyl-D-alanine nd (2S,3aR,8aS)-6-chloro-3a-hydroxy-1,2,3,3a,8,8a-hexahydropyrrolo[2,3-b]indole-2-carboxylic acid. The Journal of Antibiotics (2010) 63, 231-235; doi:10.1038/ja.2010.24; published online 9 April 2010