Article
Microbiology
Darwin Linardi, Weiyi She, Qian Zhang, Yi Yu, Pei-Yuan Qian, Henry Lam
Summary: The study developed a high throughput proteomics-based method to complement genome mining methods for the identification of biosynthetic pathways in Streptomyces species.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Kavitha Stalin, Lokesh Ravi, Vimala Raghavan
Summary: Marine actinomycetes, particularly Streptomyces thermolineatus VITKV6A, are found to produce bioactive compounds with antimicrobial and antifouling activities. The active compound, identified as Oxycyclopentadien, exhibits significant antimicrofouling and antimacrofouling properties, making it a promising candidate for potential applications.
ENVIRONMENTAL TECHNOLOGY & INNOVATION
(2022)
Article
Chemistry, Multidisciplinary
Jia-Xuan Yan, Qihao Wu, Mitasree Maity, Doug R. Braun, Imraan Alas, Xiao Wang, Xing Yin, Yanlong Zhu, Bailey A. Bell, Scott R. Rajski, Ying Ge, Douglas D. Richardson, Wendy Zhong, Tim S. Bugni
Summary: Cyclic peptides are a promising source of drug leads, but their structural complexity poses challenges for structure elucidation. Isotopic fine structure analysis, NMR, and mass spectrometry techniques were used to determine the sequence and stereochemistry of streptnatamide A, a cyclic peptide from a marine-derived Streptomyces sp. The analysis also tentatively identified and annotated a biosynthetic gene cluster for streptnatamide A based on genomic sequencing data. These analytical tools are valuable for overcoming the challenges of cyclic peptide structure elucidation and accelerating the development of bioactive cyclic peptides.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Article
Biotechnology & Applied Microbiology
Jiequn Wu, Danqing Chen, Jinrong Wu, Xiaohe Chu, Yongmei Yang, Lina Fang, Wei Zhang
Summary: The Crp gene in the industrial strain of S. roseosporus plays a key role in daptomycin production, with the deletion of Crp causing a significant decrease in daptomycin yield and overexpression leading to an increase in production; Transcriptome and qPCR analysis revealed that Crp can regulate the expression of genes related to the daptomycin biosynthetic gene cluster and the pleiotropic regulator.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Chemistry, Organic
Shingo Takiguchi, Yuki Hirota-Takahata, Takahide Nishi
Summary: The absolute configuration of ogipeptins has been elucidated by the successful total synthesis of ogipeptin A.
Article
Biology
Bing-Bing Ma, Chen-Fan Sun, Jing-Yi Zhou, Shuai-Lei Gu, Xin-Yi Dai, Yan-Zhen Chen, Qing-Wei Zhao, Xu-Ming Mao
Summary: Protein post-translational modifications (PTMs) with various acyl groups in Streptomyces can be further modified, known as post-PTM modification (PPM). By oxidizing the crotonyl groups of an acetyl-CoA synthetase Acs, the protein LimB in Streptomyces roseosporus regulates crotonylation level, morphological development, and antibiotic production.
COMMUNICATIONS BIOLOGY
(2023)
Article
Chemistry, Physical
Xingfan Li, Feng Wei, Xiaosong Le, Li Wang, Dong Wang, Cuixia Chen, Shijian Xu, Xiaotian Liao, Yurong Zhao
Summary: The morphology of self-assemblies formed by peptide molecules can be influenced by solvents and peptide molecular structure. The morphologies formed in methanol are significantly different from those formed in water.
JOURNAL OF MOLECULAR LIQUIDS
(2022)
Article
Microbiology
Joon-Sun Choi, Yeong-Jae Seok, You-Hee Cho, Jung-Hye Roe
Summary: This study demonstrates that iron supplementation promotes intrinsic resistance of aerobic actinomycetes against bactericidal antibiotics by increasing respiration, especially in a mode of using less oxygen to cope with oxidative damage caused by antibiotics.
Article
Biotechnology & Applied Microbiology
Xin Liu, Bin Liu, Xiaolei Wei, Yaping Shi, Airong Jia, Changyun Wang
Summary: The study isolated two fucoidan fractions (SPF-1 and SPF-2) from S. pallidum, which were characterized by high-performance gel permeation chromatography and gas chromatography. It was found that SPF-1 and SPF-2 mainly consists of fucose, mannose, and galactose, with small amounts of glucose, xylose, and rhamnose, and displayed different cytotoxic activities against P388 and A549 cells.
JOURNAL OF APPLIED PHYCOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Qian Zhang, Hao-Tian Chi, Linrui Wu, Zixin Deng, Yi Yu
Summary: The biosynthesis of apramycin involves phosphorylation, deacetylation, and dephosphorylation steps with the enzymes AprU, AprP, and AprZ. Streptomyces tenebrarius uses C-5 phosphorylation and N-7' acetylation to avoid auto-toxicity of apramycin. This study provides insights for designing new generation AGAs to combat drug-resistant pathogens.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Microbiology
Olha Schneider, Martin Zehl, Reiko Ueoka, Christian Ruckert, Tobias Buche, Yi Jiang, Qin-Yuan Li, Jorn Piel, Sergey B. Zotchev
Summary: In their natural habitats, bacteria encounter a wide variety of organic compounds, including antibiotics produced by other members of the microbial community. This study identified and characterized an enzyme that is apparently involved in the biosynthesis of a siderophore, but also has the ability to modify and inactivate structurally diverse antibiotics. This discovery highlights additional capabilities of bacteria to withstand antibiotic treatment and suggests that enzymes involved in secondary metabolism may have an additional function in the natural environment.
Article
Chemistry, Organic
Qing Chen, Ming-hao Wu, Qi Chang, Xia Zhao
Summary: The new marine antibacterial cyclic hexapeptide Bacicyclin (1) and its 6 analogues were successfully synthesized to investigate the relationship between structure and activity. The study also revealed that unnatural amino acids with aryl fluorosulfate group have the potential to enhance the anti-tumor activity of cyclic peptides.
TETRAHEDRON LETTERS
(2021)
Article
Infectious Diseases
Ramon Santamaria, Ana Martinez-Carrasco, Jesus Martin, Jose R. Tormo, Ignacio Perez-Victoria, Ignacio Gonzalez, Olga Genilloud, Fernando Reyes, Margarita Diaz
Summary: Xylem sap from grapevines has a positive effect on antibiotic production in Streptomyces bacteria, inducing the production of known compounds and new unidentified compounds. The active fraction of the sap contains small molecules that act as elicitors, but there may be other unidentified elicitors involved.
Article
Chemistry, Physical
Lauren Matthews, Theyencheri Narayanan
Summary: Self-assembled lyotropic phases, particularly microemulsions, play a crucial role in formulation science. By systematically increasing the bending modulus of the surfactant film, microemulsions can be made to swell and form various different phases.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2022)
Article
Thermodynamics
J. H. F. de Jesus, R. S. Medeiros, J. A. Ellena, E. T. G. Cavalheiro
Summary: The thermal behavior and polymorphism of tert-butylhydroquinone (TBHQ) were studied using differential scanning calorimetry, infrared spectroscopy, and hot stage microscopy. Two polymorphs of TBHQ were identified depending on the thermal treatment: a commercial form and a new form obtained from melt-crystallization.
JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY
(2023)
