Article
Food Science & Technology
Ill-Min Chung, Yun-Ju Kim, Chang Kwon, Hee-Sung Moon, Jae-Gu Han, Won-Sik Kong, Seung-Hyun Kim
Summary: This study highlights the limitations of the current origin labeling system for non-soil-based agricultural products, particularly mushrooms. A new origin identification model based on stable isotope ratio analysis is proposed to accurately determine the geographical origin of shiitake mushrooms, with a high classification sensitivity and overall accuracy. This suggests the need for revising the current labeling system to ensure fair trade and prevent mislabeling in the global shiitake market.
NPJ SCIENCE OF FOOD
(2021)
Article
Food Science & Technology
Anna-Akrivi Thomatou, Eleni Psarra, Eleni C. Mazarakioti, Katerina Katerinopoulou, Georgios Tsirogiannis, Anastasios Zotos, Achilleas Kontogeorgos, Angelos Patakas, Athanasios Ladavos
Summary: Consumers are increasingly interested in the geographical origin of the food they consume, and stable isotope analysis can be used to differentiate the geographical origin of bottarga products, ensuring their authenticity.
Review
Nutrition & Dietetics
R. J. Scott Lacombe, Richard P. Bazinet
Summary: Differences in carbon assimilation pathways among plants lead to variations in carbon isotope ratios in foods and animal products, which have been underappreciated in nutrition. Recent studies have shown that carbon isotope ratio analysis can serve as an effective tool for evaluating nutritional biomarker status, not only for sugar and animal protein intake, but also for studying fatty acid metabolism.
Article
Biochemical Research Methods
Thomas Piper, Mario Thevis
Summary: The determination of carbon isotope ratios (CIR) is essential in detecting misuse of testosterone (T) or boldenone (Bo) in doping control analysis. Some pharmaceutical preparations may complicate the current application of CIR in sports drug testing by showing enriched isotope ratios. Research on samples seized in Switzerland and Germany showed that most boldenone preparations had expected CIR values, while a minority of testosterone samples exhibited atypical values.
DRUG TESTING AND ANALYSIS
(2022)
Article
Biochemical Research Methods
Jochen Vogl
Summary: A new calibration approach has been developed to obtain absolute isotope ratios of multi-isotopic elements by comparing the instrumental isotope fractionation of different mass spectrometers. The theory was tested on Cd and Pb isotope ratios, showing results compatible with certified ratios but with a slight bias that needs improvement. The method is universal and can be applied to all elements with three or more isotopes, providing information on the fractionation process itself.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
David Baliu-Rodriguez, Benjamin J. Stewart, Ted J. Ognibene
Summary: Accelerator mass spectrometry (AMS) is a highly sensitive method for quantitating low amounts of 14C-labeled biomolecules, but it cannot provide structural information about the analyte. Liquid sample-AMS (LS-AMS) combines AMS with a molecular mass spectrometer to provide simultaneous quantitative AMS and structural MS data. The method was validated using 14C-labeled tryptophan and demonstrated by analyzing yeast cell extracts.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2023)
Article
Chemistry, Applied
Philippe Tixier, Denis Loeillet, Mathieu Coulis, Thierry Lescot, Luc de Lapeyre de Bellaire
Summary: With the increasing production of organic bananas, it is crucial to monitor compliance with organic specifications. This study found that the delta N-15 values of organic bananas were significantly higher than conventional ones at farms around the world, while the type of fertilization did not significantly affect delta C-13 values.
Article
Biochemical Research Methods
Charlotte E. Bopp, Jakov Bolotin, Sarah G. Pati, Thomas B. Hofstetter
Summary: Monitoring stable oxygen isotope ratios provides insights into fundamental processes in bio(geo)chemistry and environmental sciences. However, interference from argon can lead to overestimation of oxygen isotope signatures, requiring instrumental modifications and post-measurement corrections.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Food Science & Technology
Kiattipong Kamdee, Sasiwimon Naksuriyawong, Nichtima Uapoonphol, Ratchai Fungklin, Jeerawat Esor, Vorapot Permnamtip, Sang-arun Meepho, Kunchit Judprasong
Summary: Honey in Thailand is used in various commercial products and it is challenging to control its production quality due to its multiple sources. Adulteration with undeclared added sugars is a common problem in the honey industry. This study found that 60% of the commercial honey samples collected contained undeclared added sugars.
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Food Science & Technology
Amelie Sina Wilde, Tomas Strucko, Casper Rastgoie Veje, Uffe Hasbro Mortensen, Lene Duedahl-Olesen
Summary: Vanillin, the most characteristic compound in vanilla flavour, is mainly produced synthetically or biosynthetically due to the high cost of vanilla pods. Different isotopic ratios, especially 813C and 82H values, can be used for authentication of vanillin, with glucose-derived vanillin showing distinct isotopic composition.
Article
Biochemical Research Methods
Momoka Suto, Hiroto Kawashima
Summary: An analytical procedure was developed to determine the delta C-13 values of organic acids in sake and wine, achieving high recovery rates and accuracies. The study identified the addition of lactic acid during sake production and the common source of tartaric acid and malic acid in wine samples originated from grapes.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Applied
Huiping Ye, Juan Yang, Gengsheng Xiao, Yan Zhao, Zhanming Li, Weidong Bai, Xiaofang Zeng, Hao Dong
Summary: This review focuses on the current research status of authenticity and geographical origin traceability of animal-derived food. It covers various aspects such as geographical origin, feeding ingredients, adulteration of substitutes, and physical and chemical properties. Stable isotope ratio analysis and element analysis are commonly used traceability techniques, while spectroscopic techniques have the advantages of quick response, low cost, and non-destructiveness. Instrument technology combined with chemometrics is the key method for origin traceability and authenticity. There is also a new trend of origin traceability through analyzing inedible parts of animal-derived food. Understanding the authenticity and geographical origin traceability of animal-derived food is of great importance for food safety.
Article
Medicine, Legal
Yang Huang, Jun Zhu, Can Hu, Hongcheng Mei, Ziyang Yu, Hao Qin
Summary: The study demonstrates that chloroform can effectively remove sulfur from black powder, and the results of stable isotope analysis after desulfurization are reliable and stable.
FORENSIC SCIENCE INTERNATIONAL
(2022)
Article
Biochemical Research Methods
Aurelien Cuchet, Anthony Anchisi, Frederic Schiets, Yohann Clement, Pierre Lanteri, Christelle Bonnefoy, Patrick Jame, Elise Carenini, Herve Casabianca
Summary: Neroli essential oil, extracted from bitter orange blossoms, is one of the most expensive natural products on the market due to its low yield and use in fragrance compositions. Various authentication strategies have been developed to identify adulterations, but further analytical improvements are needed for precision. Isotopic profiling and enantioselective analysis have proven effective for geographical tracing and identifying adulterations in neroli essential oil.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2021)
Article
Chemistry, Analytical
William Kew, Rene M. Boiteau, John M. Eiler, Ljiljana Pasa-Tolic, James J. Moran
Summary: Subtle variations in stable isotope ratios at natural abundance provide critical insights into various processes. Fourier transform mass spectrometry, particularly Orbitrap mass spectrometry, has shown promising results in accurately and precisely measuring the natural abundance isotope ratios. It can be used for both small compounds and larger molecules, and can also be combined with online liquid chromatography.
ANALYTICAL CHEMISTRY
(2023)
Article
Food Science & Technology
Imad Kabach, Najat Bouchmaa, Reda Ben Mrid, Zakia Zouaoui, Mohammed El Maadoudi, Ayoub Kounnoun, Fadoua Asraoui, Fouad El Mansouri, Abdelmajid Zyad, Francesco Cacciola, Yassine Oulad El Majdoub, Luigi Mondello, Mohamed Nhiri
Summary: This study investigated the phytochemical composition, antioxidant properties, antidiabetic potential, and cytotoxicity of Olea europaea var. oleaster (Oeo) fruit. The results showed that Oeo fruit contains high levels of phenolic and flavonoid compounds and exhibits strong antioxidant activity. Methanolic extract demonstrated better antioxidant activity than aqueous extract and showed inhibitory effects on glycation and breast cancer cells.
JOURNAL OF FOOD MEASUREMENT AND CHARACTERIZATION
(2023)
Article
Chemistry, Applied
Mustapha Cherrate, Reda EL-Mernissi, Aziz Bouymajane, Fouzia Rhazi Filali, Emanuela Trovato, Ghizlane Echchgadda, Abdellah Maissour, Mohammed Aziz Ajana, Paola Dugo, Francesco Cacciola, Luigi Mondello, Kacem Makroum
Summary: The study focused on characterizing the chemical composition and assessing the antioxidant and antibacterial activities of Ziziphora hispanica essential oil (ZH-EO) from the Middle Atlas of Morocco (Boulmane). Results showed that ZH-EO consisted of 119 volatile components, representing 88.7% of the total oil. ZH-EO exhibited bactericidal and bacteriostatic effects against various bacteria and demonstrated strong antioxidant activity. ADMET prediction indicated favorable pharmacokinetic properties of the tested components. This study suggests that Ziziphora hispanica essential oil could be a potential source of bioactive molecules for preventing diseases related to oxidative stress and pathogenic bacteria.
JOURNAL OF ESSENTIAL OIL RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Nadia Naim, Aziz Bouymajane, Yassine Oulad El Majdoub, Said Ezrari, Rachid Lahlali, Abdessalem Tahiri, Said Ennahli, Roberto Lagana Vinci, Francesco Cacciola, Luigi Mondello, Ilham Madani
Summary: This study investigates the phenolic composition and antibacterial properties of hydroethanolic extracts from Crocus sativus L. petals collected in Serghina, Morocco. The extracts were found to contain 27 phenolic compounds, with the most abundant being kaempferol-sophoroside isomer. The extracts showed bactericidal effects against Staphylococcus aureus and Listeria monocytogenes, and bacteriostatic effects against Escherichia coli and Salmonella typhimurium. These findings suggest that Crocus sativus L. petal extracts could be valuable sources of natural antibacterial agents for the food and pharmaceutical industries.
Article
Food Science & Technology
Ghanya Al-Naqeb, Cinzia Cafarella, Eugenio Aprea, Giovanna Ferrentino, Alessandra Gasparini, Chiara Buzzanca, Giuseppe Micalizzi, Paola Dugo, Luigi Mondello, Francesca Rigano
Summary: This study evaluated the potential of supercritical fluid extraction (SFE) as a green alternative to Soxhlet extraction for obtaining oils from Opuntia ficus-indica (OFI) and Opuntia dillenii (OD) seeds. SFE with specific parameters produced oils rich in unsaturated fatty acids and tocopherols. The highest yield was achieved through Soxhlet extraction, and the most abundant fatty acid in all samples was linoleic acid. SFE of OFI seeds from Yemen resulted in the highest gamma-tocopherol content. Overall, SFE demonstrated its suitability for producing oils with potential applications in functional food and nutraceutical products.
Article
Food Science & Technology
Marianna Oteri, Giovanni Bartolomeo, Francesca Rigano, Juan Aspromonte, Emanuela Trovato, Giorgia Purcaro, Paola Dugo, Luigi Mondello, Marco Beccaria
Summary: This study comprehensively characterized the chemical components of Chia seed oil, including fatty acid methyl esters (FAMEs), triacylglycerols (TAGs), phospholipids (PLs), free fatty acids (FFAs), sterols, carotenoids, tocopherols, and polyphenols. Different analytical methods such as reversed phase liquid chromatography (RP-LC), gas chromatography (GC), and mass spectrometry (MS) were employed for the analysis of these components. The results showed that Chia seed oil contained high levels of antioxidant tocopherols and essential fatty acids, especially alpha-linolenic acid and linoleic acid. Additionally, this study provided new insights into the composition of FFAs and certain classes of carotenoids in Chia seed oil.
Article
Chemistry, Analytical
Tania Maria Grazia Salerno, Marianna Oteri, Paola Arena, Emanuela Trovato, Danilo Sciarrone, Paola Donato, Luigi Mondello
Summary: Supercritical fluid chromatography (SFC) has gained renewed interest, thanks to advancements in hardware capabilities and tailored packing materials. The use of a subcritical co-solvent mixed with carbon dioxide has expanded the range of SFC-amenable compounds, including lipids. This research developed a subFC method for separating triacylglycerols (TAGs) in various vegetable oils, achieving efficient separations with reduced analysis time and solvent consumption.
Article
Medicine, Research & Experimental
Imad Kabach, Najat Bouchmaa, Zakia Zouaoui, Abdelhamid Ennoury, Sara El Asri, Abdelmounaim Laabar, Loubna Oumeslakht, Francesco Cacciola, Yassine Oulad El Majdoub, Luigi Mondello, Abdelmajid Zyad, Naima Nhiri, Mohamed Nhiri, Reda Ben Mrid
Summary: The study aimed to evaluate the antioxidant and antidiabetic properties of Oxalis pes-caprae flowers extract in alloxan-induced diabetic mice. Results showed that the extract exhibited significant antioxidant and antidiabetic activity by reducing blood glucose levels and increasing the activities of antioxidants and glycolysis enzymes.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Biochemistry & Molecular Biology
Paola Arena, Natalizia Miceli, Andreana Marino, Federica Davi, Emilia Cavo, Vivienne Spadaro, Francesco Maria Raimondo, Francesco Cacciola, Roberto Lagana Vinci, Luigi Mondello, Maria Fernanda Taviano
Summary: This study aimed to explore Sinapis pubescens subsp. pubescens as a new potential source of active metabolites. Comparative analysis was conducted on the hydroalcoholic extracts of the leaf, flower, and stem. Polyphenols in the extracts were quantitatively determined and characterized, revealing significant differences in their profiles. The extracts exhibited antioxidant activity, with the leaf extract showing the best radical scavenging activity and the flower extract demonstrating the highest chelating activity. However, no antimicrobial activity was observed in the strains tested. Moreover, the extracts were found to be non-toxic. The aerial parts of S. pubescens subsp. pubescens could serve as valuable sources of antioxidants for pharmaceutical and nutraceutical applications.
CHEMISTRY & BIODIVERSITY
(2023)
Article
Food Science & Technology
Emanuela Trovato, Marina Russo, Lorenzo Cucinotta, Yassine Oulad El Majdoub, Maria Rita Testa Camillo, Gemma De Grazia, Adriana Arigo, Danilo Sciarrone, Luigi Mondello, Paola Dugo
Summary: Flavoured EVOOs in the niche market of extra-virgin olive oil are enriched with aromatic and medicinal plants, which affect their chemical composition. Analytical techniques including HPLC and GC methods were used to investigate the qualiquantitative profile of molecules in the volatile and non-volatile fractions of flavoured EVOOs. The results confirmed the genuineness of most flavoured EVOOs, with a few requiring additional analytical devices for further authentication.
FOOD ANALYTICAL METHODS
(2023)
Article
Chemistry, Applied
Emanuela Trovato, Katia Arena, Roberta La Tella, Francesca Rigano, Roberto Lagana Vinci, Paola Dugo, Luigi Mondello, Paolo Guarnaccia
Summary: This study provides a comprehensive characterization of minor components in hemp seed-based food products, including volatile and non-volatile metabolites. Terpenes were found to be the most abundant compounds among volatiles, while hempseed oils contained 58 compounds in the unsaponifiable matter. Phenols and cannabinoids were also investigated, with a total of 52 compounds identified and quantified.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2023)
Article
Food Science & Technology
Ahlem Eddehech, Renaud Rahier, Danilo Donnarumma, Francesca Rigano, Alexandre Noiriel, Abdelkarim Abousalham, Francesco Cacciola, Luigi Mondello, Zied Zarai
Summary: This study presents the expression and characterization of a novel phosphatidylinositol phospholipase C (PI-PLCBt) from Bacillus thuringiensis PL14, which shows potential for oil degumming. Enzymatic degumming tests were conducted on crude soybean oil using rPI-PLCBt, and its efficiency was evaluated by measuring phosphorus and diacylglycerol (DAG) contents after different treatments. The results showed that enzymatic degumming achieved a high efficiency of 89.4%, reducing phosphorus content to 13.7 mg kg-1 and increasing DAG levels. This suggests that enzymatic degumming could be a more efficient and environmentally friendly alternative to traditional degumming methods, while also enhancing the nutritional value of the final product.
Article
Integrative & Complementary Medicine
Csongor Bako, Viktoria Lilla Balazs, Erika Kerekes, Bela Kocsis, David U. Nagy, Peter Szabo, Giuseppe Micalizzi, Luigi Mondello, Judit Krisch, Dora Petho, Gyorgyi Horvath
Summary: Thyme essential oil is popular due to its antimicrobial effect, but the chemical composition of the oil influences its biological activity. This study investigates the influence of flowering phenophases on the chemical composition, antibacterial and anti-biofilm activity of Thymus vulgaris essential oil, finding that the collection time has important implications for its activity.
BMC COMPLEMENTARY MEDICINE AND THERAPIES
(2023)
Article
Chemistry, Applied
Natalia Manousi, Antonio Ferracane, Natasa P. Kalogiouri, Abuzar Kabir, Kenneth G. Furton, Peter Q. Tranchida, George A. Zachariadis, Luigi Mondello, Victoria F. Samanidou, Erwin Rosenberg
Summary: In this study, sol-gel sorbent-coated second-generation fabric phase sorptive extraction (FPSE) membranes were synthesized using titania-based sol-gel precursors. These membranes showed high efficiency in extracting organophosphorus pesticides from apple juice samples. The optimized FPSE method using these membranes demonstrated good accuracy in analysis and the novel membranes were reusable up to 5 times. Comparatively, the titania-based membranes provided higher extraction recoveries than conventional silica-based membranes.
Article
Biochemical Research Methods
Alessia Arena, Antonio Ferracane, Mariosimone Zoccali, Markus Obkircher, Hanspeter Sprecher, Peter Q. Tranchida, Luigi Mondello
Summary: This study presents a rapid and limited-volume solvent-extraction sample preparation method for pesticide determination in hemp seed oil. Compared to previous research, this study extends the range of pesticides and investigates a more challenging matrix.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Biochemical Research Methods
Laura Martin-Pozo, Katia Arena, Francesco Cacciola, Paola Dugo, Luigi Mondello
Summary: Due to concerns over the use of chemicals and pesticides, a comprehensive method using two-dimensional liquid chromatography coupled with tandem mass spectrometry was proposed for the analysis of 112 pesticides in corn-based products. The method employed a reduced QuEChERS-based extraction and clean-up procedure, and achieved low limits of quantification and precision values. Real samples were analyzed and three pesticides were detected at trace levels. This work paves the way for the treatment of complex matrices such as corn products.
JOURNAL OF CHROMATOGRAPHY A
(2023)
