Article
Nutrition & Dietetics
Przemyslaw Lukasz Kowalczewski, Anna Olejnik, Martyna Natalia Wieczorek, Joanna Zembrzuska, Katarzyna Kowalska, Jacek Lewandowicz, Grazyna Lewandowicz
Summary: More and more literature data show the health benefits of potato juice, but it is not clear which compounds in potato juice are biologically active. This study aimed to determine the antiproliferative effect of digested potato juice and its processing products. The results showed that glycoalkaloids exhibited cytotoxicity against all cell lines studied, while phenolic acids did not show cytotoxicity. There was a correlation between cytotoxic potency and glycoalkaloids content in all potato juice products.
Article
Microbiology
Dorota Soltys-Kalina, Anna Grupa-Urbanska, Renata Lebecka, Maud Tallant, Isabelle Kellenberger, Brice Dupuis
Summary: This article explores the possibility of using glycoalkaloids (GAs) produced by potato tubers after the greening process as a control method for blackleg disease caused by Dickeya and Pectobacterium species. The study found that GAs extracted from four potato cultivars exhibited bacteriostatic and bactericidal effects against both bacteria strains in growth media. Field experiments showed that plants grown from green seed tubers had lower incidence of blackleg, except for one-year trial. However, the control effect was marginal, possibly due to low production of GAs by cv. Agria tubers after greening.
Article
Biotechnology & Applied Microbiology
Guangji Ye, Zhenzhen Zheng, Yun Zhou, Xiuqin Pu, Wang Su, Heng Guo, Jian Wang
Summary: The MYB transcription factor LrAN2 from Lycium ruthenicum regulates the biosynthesis of anthocyanins and glycoalkaloids in potato, offering a new breeding strategy for potato improvements.
PLANT CELL TISSUE AND ORGAN CULTURE
(2021)
Article
Agronomy
Wenqian Wang, Guanli Xiao, Guangzu Du, Lvshu Chang, Yun Yang, Jvhui Ye, Bin Chen
Summary: It has been discovered that the gut bacteria Glutamicibacter halophytocola S2 in the potato tuber moth can degrade the toxic substances alpha-solanine and alpha-chaconine in potatoes. This enhances the adaptability of the moth on its host, potatoes.
PEST MANAGEMENT SCIENCE
(2022)
Article
Agronomy
Jose Sanchez del Pulgar, Massimo Lucarini, Altero Aguzzi, Paolo Gabrielli, Bruno Parisi, Daniela Pacifico, Giuseppe Mandolino, Ginevra Lombardi-Boccia
Summary: The study evaluated the glycoalkaloid content in six hybrid potato clones from an Italian breeding programme, finding significant variability in content among clones. While genotypic variation was the main factor influencing glycoalkaloid accumulation, the study also highlighted the influence of agro-climatic conditions on the content.
Article
Chemistry, Multidisciplinary
Przemyslaw Lukasz Kowalczewski, Joanna Zembrzuska, Agnieszka Drozdzynska, Krzysztof Smarzynski, Dominika Radzikowska, Marek Kieliszek, Pawel Jezowski, Zuzanna Sawinska
Summary: Potato juice (PJ) has diverse biological activities like anti-inflammatory, antioxidant, and cytotoxic effects. The antioxidant activity and GAs content of PJ are influenced by different potato varieties, but none of them showed antimicrobial activity.
Article
Agriculture, Multidisciplinary
Sebastian Baur, Nicole Belle, Hans Hausladen, Sebastian Wurzer, Laura Brehm, Timo D. Stark, Ralph Huecklhoven, Thomas Hofmann, Corinna Dawid
Summary: This study quantitatively investigates the changes of SGAs and saponins in potatoes induced by post-harvest LED light exposure, using advanced analytical techniques. The results show that the SGA contents in potatoes exceed the safety limit, and the levels of saponins vary significantly among different cultivars. Moreover, cultivar-specific regulations of SGAs and saponins are observed due to light exposure.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Nicolas Joly, Kaies Souidi, David Depraetere, Daniel Wils, Patrick Martin
Summary: The study examined the total phenolic compounds (TPC) and chlorogenic acids content in potato by-product extracts using different hydro alcoholic solvents and extraction methods. It found that heating-assisted extraction increased the TPC content significantly, and unpeeled potatoes had higher TPC levels with accumulation in the skin tissue. The concentration of chlorogenic acids, especially 5-CQA, was highest in methanol-extracted potato peels, with further improvement with heating-assisted extraction. The antioxidant capacity of the potato by-product extracts was found to be high, indicating the potential use of TPC as an indicator for the antioxidant properties of potato by-products.
Article
Chemistry, Applied
Sebastian Baur, Oliver Frank, Hans Hausladen, Ralph Huckelhoven, Thomas Hofmann, Wolfgang Eisenreich, Corinna Dawid
Summary: This study elucidated the biosynthetic pathways of major glycoalkaloids (SGAs) in potatoes using (CO2)-C-13 labeling experiments, showing that the biosynthesis of these SGAs is related to mevalonate.
Article
Agronomy
Hong Li, Ming Li, Yanling Fan, Yuhui Liu, Shuhao Qin
Summary: By analyzing the content of glycoalkaloids (PGAs) in green potato peel using HPLC, it was found that the average content of PGAs per kilogram of green potato peel was 2238.76 mg, with alpha-chaconine being about 1.8 times higher than alpha-solanine. In vitro experiments showed that PGAs could inhibit the mycelium growth of the potato dry rot pathogen F. sulphureum on potato dextrose agar medium, and the inhibition rate was positively correlated with PGAs concentration. Furthermore, PGAs were also able to reduce the contents of malondialdehyde, total phenols, flavonoids, as well as the activities of peroxidase and superoxide dismutase induced by F. sulphureum by inhibiting or killing part of the mycelium. These findings indicate that PGAs can serve as a promising botanical fungicide to partially replace synthetic fungicides in the disease management of potato.
Article
Agriculture, Dairy & Animal Science
Annette Lykke Voergaard, Leonardo Victor de Knegt, Johannes Gulmann Madsen, Jacob Dall
Summary: The inclusion of standard PPC with increased levels of glycoalkaloids in piglet diets does not negatively affect growth performance or fecal score.
Article
Engineering, Chemical
Thanh-Tri Nguyen, Carmen Rossello, Cristina Ratti
Summary: This study characterized the air-drying kinetics of potato peel waste and established its sorption isotherms. The proximate composition, tissue structure, phenolics, and chlorogenic acid contents of fresh and dried potato peel samples were determined. The drying kinetics curves and sorption isotherms were well fitted by mathematical models. The results highlight the importance of selecting appropriate drying parameters for further transformation of potato waste.
Review
Biochemistry & Molecular Biology
Giuseppe Di Pede, Pedro Mena, Letizia Bresciani, Mariem Achour, Rosa M. Lamuela-Raventos, Ramon Estruch, Rikard Landberg, Sabine E. Kulling, David Wishart, Ana Rodriguez-Mateos, Michael N. Clifford, Alan Crozier, Claudine Manach, Daniele Del Rio
Summary: This work systematically assessed the pharmacokinetics, urinary recovery, and bioavailability of hydroxycinnamic acids (HCAs) and their metabolites. It was found that C-6-C-3 cinnamic acids, such as caffeic and ferulic acid, had the highest blood concentrations, while their excretion in urine was lower than hydroxybenzene catabolites. A comprehensive study investigating the ADME of HCAs from different dietary sources is urgently required.
ANTIOXIDANTS & REDOX SIGNALING
(2023)
Review
Food Science & Technology
Evzen Sarka, Marcela Slukova, Svatopluk Henke
Summary: This paper focuses on the retention of phenolics in extrudates and their health effects, with particular attention to the changes in individual phenolics during extrusion. The study also highlights the importance of considering other physico-chemical changes in input materials when designing an extrusion process.
Article
Chemistry, Applied
Haitian Fang, Xiuxiu Yin, Jiequn He, Shihua Xin, Huiling Zhang, Xingqian Ye, Yunyun Yang, Jinhu Tian
Summary: The study investigated the effects of different cooking methods on the phytochemicals and antioxidant activities of potatoes from different varieties. The results showed that frying, roasting, and air-drying had a more significant negative impact on the nutrients and antioxidant activities of potatoes compared to steaming or microwaving. Zhongshubahao variety may be an ideal potato sample in terms of retaining phytochemicals and antioxidant activities.
Article
Food Science & Technology
Sara Bussler, Harshadrai M. Rawel, Oliver K. Schlueter
INNOVATIVE FOOD SCIENCE & EMERGING TECHNOLOGIES
(2020)
Article
Food Science & Technology
An Borremans, Sara Bussler, Sorel Tchewonpi Sagu, Harshadrai Rawel, Oliver K. Schluter, Van Campenhout Leen
Article
Food Science & Technology
Gustavo A. Figueroa Campos, Sorel Tchewonpi Sagu, Pedro Saravia Celis, Harshadrai M. Rawel
Article
Food Science & Technology
Sorel Tchewonpi Sagu, Lynn Zimmermann, Eva Landgraeber, Thomas Homann, Gerd Huschek, Haydar Ozpinar, Florian J. Schweigert, Harshadrai M. Rawel
Article
Food Science & Technology
Bibiana Silva, Ana Carolina Oliveira Costa, Sorel Sagu Tchewonpi, Josephine Boenick, Gerd Huschek, Luciano Valdemiro Gonzaga, Roseane Fett, Susanne Baldermann, Harshadrai M. Rawel
Summary: The study developed a proteomics strategy to establish specific markers for quantifying proteins in honey, especially for distinguishing honeydew honeys from floral honeys. By quantifying selected marker peptides, such as QNIDVVAR, the study showed the potential of using specific protein content as a marker for authenticating honeydew honeys. Ultimately, principal components analysis highlighted the content of QNIDVVAR as a good descriptor for distinguishing different honey samples.
FOOD RESEARCH INTERNATIONAL
(2021)
Article
Biochemistry & Molecular Biology
Alexandra Wetzel, Bettina Scholtka, Fabian Schumacher, Harshadrai Rawel, Birte Geisendoerfer, Burkhard Kleuser
Summary: Research has shown that upregulation of EBI3 through histone acetylation can alleviate symptoms in a mouse model of chronic colitis. Further investigation revealed that under inflammatory conditions, treatment with DNA methylation inhibitors and TNF alpha led to synergistic upregulation of EBI3 in human colon epithelial cells. The results suggest that the upregulation of EBI3 under inflammatory and demethylating conditions may lead to the formation of anti-inflammatory IL-35, which could be a potential therapeutic target for colitis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Entomology
Sorel Tchewonpi Sagu, Eva Landgraeber, Ina M. Henkel, Gerd Huschek, Thomas Homann, Sara Bussler, Oliver K. Schlueter, Harshadrai Rawel
Summary: The study investigates the potential effects of cereal alpha-amylase/trypsin inhibitors (ATIs) on growth parameters and selective digestive enzymes of Tenebrio molitor larvae. The results show that meals with different ATI levels can impact larval development and digestive enzyme expression, with higher ATI content resulting in increased larval death rates.
Article
Biochemistry & Molecular Biology
Sorel Tchewonpi Sagu, Gerd Huschek, Thomas Homann, Harshadrai M. Rawel
Summary: Detection and quantification of nut allergens pose a challenge, with liquid chromatography tandem mass spectrometry (LC-MS/MS) emerging as a widely used method. Different extraction and digestion protocols were optimized for different nuts, with SDS buffer extraction suitable for almond, peanut, and walnut samples, and PVPP buffer extraction recommended for cashew, pistachio, and hazelnut samples. Various methods were assessed for protein extraction and digestion efficiency in the study.
Article
Engineering, Chemical
Gustavo A. Figueroa Campos, Jeffrey Paulo H. Perez, Inga Block, Sorel Tchewonpi Sagu, Pedro Saravia Celis, Andreas Taubert, Harshadrai M. Rawel
Summary: The valorization of coffee wastes through modification to activated carbon has the potential to compete with commercial carbons. Activated carbons prepared from spent coffee grounds and parchment showed lower adsorption capacity for lactic acid compared to standard commercial granular activated carbon. These results demonstrate the potential of using coffee by-products as low-cost adsorbents for removing organic compounds from aqueous solutions.
Article
Engineering, Chemical
Sorel Tchewonpi Sagu, Gerd Huschek, Tess Waldbach Braga, Michal Rackiewicz, Thomas Homann, Harshadrai M. Rawel
Summary: In this study, a simplified approach based on reverse phase and ion exchange chromatography was utilized to purify the major wheat alpha-amylase/trypsin inhibitors (ATIs). The efficiency of the purification process was demonstrated by obtaining pure fractions of the targeted ATIs with high purity levels. These purified ATIs can now be further studied to gain more knowledge about their involvement in non-celiac sensitivity.
Article
Food Science & Technology
Dirk W. Lachenmeier, Steffen Schwarz, Joerg Rieke-Zapp, Ennio Cantergiani, Harshadrai Rawel, Maria Angeles Martin-Cabrejas, Maria Martuscelli, Vera Gottstein, Simone Angeloni
Summary: This article discusses the potential of coffee by-products and their impact on coffee farmers and the environment. By-products can be utilized for human nutrition, contributing to sustainability and waste reduction. However, there are restrictions in this field that require further research and policy support.
Article
Food Science & Technology
Gustavo A. Figueroa Campos, Johannes G. K. T. Kruizenga, Sorel Tchewonpi Sagu, Steffen Schwarz, Thomas Homann, Andreas Taubert, Harshadrai M. Rawel
Summary: This study investigated the changes in the protein fraction of coffee, which is important for cup quality. The results showed a significant increase in modified peptides from wet processed green beans compared to dry ones. This study contributes to a better understanding of the influence of different processing methods on protein quality and its role in coffee cup quality and aroma.
Article
Biochemistry & Molecular Biology
Sorel Tchewonpi Sagu, Harshadrai M. Rawel, Sascha Rohn
Summary: This study investigated the post-translational modification (PTM) of kappa-casein (kappa-CN) during bovine milk fermentation. A LC-MS method was developed to identify and quantify the modified and unmodified variants of four peptides containing serine and threonine residues. The results showed that over 50% of kappa-CN existed in one of its modified forms in milk, and fermentation significantly altered the composition of unmodified/modified kappa-CN.
Article
Food Science & Technology
Mostafa Ali, Harshadrai Rawel, Michael Hellwig
Summary: The objective of this study was to improve the functional properties of green coffee protein. Extraction of defatted green coffee meal using PVPP increased protein yield and decreased the antioxidant activity of the meal. Peptic hydrolysis significantly increased the degree of hydrolysis, and enzymatic hydrolysis improved the antioxidant activity of the green coffee protein. The results suggest that enzymatic hydrolysis with pepsin is an effective technique to obtain bioactive compounds.
JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE
(2023)
Article
Food Science & Technology
Kapil Nichani, Steffen Uhlig, Bertrand Colson, Karina Hettwer, Kirsten Simon, Josephine Boenick, Carsten Uhlig, Sabine Kemmlein, Manfred Stoyke, Petra Gowik, Gerd Huschek, Harshadrai M. Rawel
Summary: Food fraud is widespread and requires innovative strategies to combat it. This study introduces a new non-targeted method (NTM) to distinguish between spelt and wheat, which is helpful in detecting fraud and testing authenticity. By using liquid chromatography coupled high-resolution mass spectrometry (LC-HRMS), a highly resolved fingerprint in the form of spectra is obtained for various cultivars of spelt and wheat. Convolutional neural network (CNN) models are built and trained using duplicate measurements of eleven cultivars, allowing them to automatically learn patterns and accurately classify samples into spelt or wheat. The results demonstrate the reliability of this approach on a wider range of cultivars.
