Article
Fisheries
Guowei Liao, Qiuping Wu, Binhua Mo, Jiang Zhou, Junyi Li, Jixing Zou, Lanfen Fan
Summary: This study investigated the impact of Vibrio alginolyticus on the intestinal histomorphology and microbiome composition in Litopenaeus vannamei. The results showed that Vibrio alginolyticus infection caused exfoliation of intestinal mucosal epithelial cells and altered the tissue morphology of the intestine. It also affected the abundance and diversity of intestinal microbiome.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Jiamin Feng, Yongxiong Huang, Meiling Huang, Junliang Luo, Liwen Que, Shiping Yang, Jichang Jian
Summary: In this study, a novel C-type lectin (CTL) called perlucin-like protein (PLP) was identified in Litopenaeus vannamei. PLP could be activated in response to Vibrio harveyi infection and could bind and agglutinate various bacteria. PLP was involved in stabilizing the expression of immune-related and apoptosis genes and reducing bacterial load in the hepatopancreas.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Fisheries
Yunhao Yuan, Haoxiang Guan, Yongxiong Huang, Junliang Luo, Jichang Jian, Shuanghu Cai, Shiping Yang
Summary: NF-E2-related factor-like-2 (Nrf2), a transcription factor belonging to the Cap'n'Collar transcription factor family, plays a role in regulating inflammation, autophagy, metabolism, proteostasis, and cancer prevention. The effects of Nrf2 on the immune response in Vibrio spp infection were investigated. The results showed that Nrf2 RNAi decreased antioxidant-related genes expression and up-regulated inflammation-related genes and apoptosis gene. Histopathological analysis revealed tissue damage in Nrf2 RNAi group, while SFN group exhibited lessened tissue damage and fewer apoptosis signals.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Chemistry, Medicinal
Xinjia Lv, Shihao Li, Yang Yu, Xiaojun Zhang, Fuhua Li
Summary: This study reveals that a crustin called LvCrustin I-2 can resist bacterial infection by regulating the balance of intestinal microbiota and has an impact on the intestinal epithelial barrier and immune function in shrimp.
Article
Fisheries
Xin Zhang, Jialong Shi, Yulong Sun, Yilei Wang, Ziping Zhang
Summary: This study discovered the immune regulation mechanism of shrimp in response to Vibrio parahaemolyticus infection after eyestalk ablation. RNASeq and KEGG analysis revealed that eyestalk ablation might activate the neuroendocrine-immune system. The expression of genes involved in the prophenoloxidase activating system and TLR signaling pathway were affected by eyestalk ablation. These findings shed light on the immune response mechanism of shrimp and provide a framework for building crustacean immunity theory.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Lian Gan, Jianwei Zheng, Wei-Hua Xu, Jianhao Lin, Jingshu Liu, Yu Zhang, Zizhan Wu, Zhaolin Lv, Youming Jia, Qingqi Guo, Shijun Chen, Chuanhe Liu, Tom Defoirdt, Qiwei Qin, Yiying Liu
Summary: This study found that bacterial contamination can cause muscle spoilage in crustaceans by changing the microbial composition, and suggested that crustaceans can be used as sensitive bio-detectors to indicate the extent of microbial contamination.
SCIENTIFIC REPORTS
(2022)
Article
Fisheries
Miao Miao, Shihao Li, Yang Yu, Fuhua Li
Summary: Two genes, LvLysM1 and LvLysM2, encoding LysM-containing proteins were identified in Litopenaeus vannamei shrimp, and their functions and potential genetic markers for disease resistance breeding were analyzed. LvLysM2 showed binding activity with different bacterial polysaccharides, while LvLysM1 did not. Knocking down LvLysM1 or LvLysM2 resulted in increased mortality of shrimp after Vibrio parahaemolyticus infection, and some SNPs in these genes were correlated with shrimp resistance to VpAHPND.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2023)
Article
Fisheries
Hongtao Liu, Shengtao Guo, Rong Wang, Yugui He, Qiong Shi, Zhaobin Song, Mingqiu Yang
Summary: The study used proteomic sequencing of hepatopancreas in Vibrio harveyi-infected whiteleg shrimp and found a significant number of DEPs, specifically showing changes in the expression levels of C-type lectins after infection. Different members of the CTL family exhibit varied responses to Vibrio harveyi infection, suggesting potential differential functions and providing theoretical guidance for further research on immune responses and pathogen infections in shrimps.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Article
Environmental Sciences
Zulaikha Mat Deris, Shumpei Iehata, Han Ming Gan, Mhd Ikhwanuddin, Musa Najiah, Md Asaduzzaman, Min Wang, Yantao Liang, Muhd Danish-Daniel, Yeong Yik Sung, Li Lian Wong
Summary: This study explores the effects of salinity levels and pathogenic Vibrio harveyi infection on the gut microbiota of Litopenaeus vannamei shrimp. The results show that shrimp cultured at a salinity of 20 ppt exhibit higher microbiota diversity, a stable bacterial network, and the presence of beneficial bacteria that help inhibit the growth of pathogens. The study concludes that a salinity of 20 ppt is optimal for suppressing the growth of V. harveyi and potential pathogenic bacteria in the shrimp gut.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Fisheries
Yuanmao Yao, Lili Shi, Wei Xiao, Sixin Guo, Saiya Liu, Haoyang Li, Shuang Zhang
Summary: PAH is a key enzyme involved in immune defense reactions by providing the starting material for synthesizing catecholamines and melanin. LvPAH was identified from Litopenaeus vannamei, showing constitutive expression with the highest levels in the eyestalk. Inhibition of LvPAH resulted in decreased survival rates and increased viral loads in L. vannamei after WSSV infection.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Immunology
Mediha Yildirim-Aksoy, Rashida Eljack, Eric Peatman, Benjamin H. Beck
Summary: This study evaluated the changes in hematology, immunology, and biochemical values in the hemolymph of shrimp infected with Vibrio parahaemolyticus. The results showed that shrimp's immune response was altered, and the hemolymph parameters could be used to assess the health status of shrimp.
MICROBIAL PATHOGENESIS
(2022)
Article
Fisheries
Xiaoli Yin, Xueqi Zhuang, Meiqiu Liao, Lin Huang, Qiqian Cui, Can Liu, Wenna Dong, Feifei Wang, Yuan Liu, Weina Wang
Summary: In this study, the transcriptomic profiles of Litopenaeus vannamei hepatopancreas and the morphological changes were investigated by injecting with bacterial extracellular products or V. alginolyticus. The transcriptome data revealed differential expression genes related to lysosome pathway, glycerophospholipid metabolism, and lipid metabolism. The findings showed that V. alginolyticus utilized lipids from L. vannamei as a nutrient source and modulated lipid homeostasis to determine the fate of infection.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Junliang Luo, Yanghui Chen, Yongxiong Huang, Jiamin Feng, Yunhao Yuan, Jichang Jian, Shuanghu Cai, Shiping Yang
Summary: In this study, a novel CTL of Litopenaeus vannamei, LvCTL7, was successfully cloned and shown to have microbial agglutination and immunoregulatory activity. LvCTL7 was mainly expressed in hepatopancreas, muscle, gill, and eyestalk, and it could bind to Gram-positive and Gram-negative bacteria. LvCTL7 was involved in the innate immune response against Vibrio infection in L. vannamei.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Environmental Sciences
Xiaohui Sun, Jia Liu, Shijing Deng, Renhe Li, Wenhua Lv, Shufeng Zhou, Xu Tang, Yun-zhang Sun, Mingyue Ke, Kunming Wang
Summary: The study found that Bacillus velezensis DH82 strain has the ability to inhibit Vibrio parahaemolyticus, regulate pathogenicity and bacterial growth, and has potential application value in aquaculture.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Immunology
Jassy Mary S. Lazarte, Young Rim Kim, Jung Seok Lee, Jin Hong Chun, Si Won Kim, Jae Wook Jung, Jaesung Kim, Pattanapon Kayansamruaj, Kim D. Thompson, Hyeongsu Kim, Tae Sung Jung
Summary: This study demonstrates that passive immunization using VLRB-based antibodies can effectively neutralize toxins associated with AHPND, providing a potential alternative to traditional immunoglobulin-based antibodies for managing AHPND outbreaks in shrimp farms.
Article
Engineering, Biomedical
Abhimanyu Thakur, Diana Carolina Parra, Pedram Motallebnejad, Marcelo Brocchi, Huanhuan Joyce Chen
Summary: Exosomes are tiny extracellular vesicles that carry various components, such as proteins, lipids, and nucleic acids, and play a crucial role in the malignant progression of cancer. Due to their ability to transport drugs and molecular information, exosomes have been considered as a novel tool for cancer therapeutics.
BIOACTIVE MATERIALS
(2022)
Article
Respiratory System
Charlotte Rigauts, Juliana Aizawa, Steven L. Taylor, Geraint B. Rogers, Matthias Govaerts, Paul Cos, Lisa Ostyn, Sarah Sims, Eva Vandeplassche, Mozes Sze, Yves Dondelinger, Lars Vereecke, Heleen Van Acker, Jodie L. Simpson, Lucy Burr, Anne Willems, Michael M. Tunney, Cristina Cigana, Alessandra Bragonzi, Tom Coenye, Aurelie Crabbe
Summary: The presence of Rothia mucilaginosa in the lower airways has an inhibitory effect on pathogen-induced inflammation and is negatively correlated with pro-inflammatory markers in bronchiectasis patients. Mechanistically, R. mucilaginosa inhibits inflammation by reducing NF-kappa B pathway activation. These findings suggest that R. mucilaginosa in the lower airways could potentially mitigate inflammation and impact the severity and progression of chronic respiratory disorders.
EUROPEAN RESPIRATORY JOURNAL
(2022)
Review
Biotechnology & Applied Microbiology
Bassirou Ndoye, Rasoul Shafiei, Nastaran Shah Sanaei, Ilse Cleenwerck, Marius K. Somda, Mamoudou Hama Dicko, Lat Souk Tounkara, Amadou Tidiane Guiro, Frank Delvigne, Philippe Thonart
Summary: This paper provides an overview of the current research on Acetobacter senegalensis at the microbial and genomic levels, as well as its specific bio-industrial applications. The strain is a thermotolerant strain isolated from mango fruits and intended for industrial vinegar fermentation processes. Additionally, the potential of this strain in other bio-industrial applications such as cocoa fermentation is also explored.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Article
Agronomy
Ho Manh Tuong, Sonia Garcia Mendez, Michiel Vandecasteele, Anne Willems, Dexian Luo, Stien Beirinckx, Sofie Goormachtig
Summary: This study isolated the SRS1 strain from salt-resistant Carex distans roots and investigated its effect on the growth of Arabidopsis and tomato. The results showed that SRS1 inoculation promoted plant growth under both saline and nonsaline conditions, including when the plants were grown in white sand and potting soil. Genetic traits related to stress alleviation from both the bacteria and the plants played a crucial role in the impact of this PGPR strain on plant performance.
Article
Plant Sciences
Khawla Oukaltouma, Ahmed El Moukhtari, Yahya Lahrizi, Bouchra Makoudi, Mohammed Mouradi, Mohamed Farissi, Anne Willems, Ahmed Qaddoury, Faouzi Bekkaoui, Cherki Ghoulam
Summary: Water deficit and phosphorus deficiency are severe limitations to agriculture productivity. This study found that phosphorus fertilization can improve the tolerance of faba bean plants to water deficit. Additionally, the combination of water deficit and phosphorus deficiency has a greater impact on plant physiology and biochemistry, with the Reina Mora variety being the most affected.
JOURNAL OF SOIL SCIENCE AND PLANT NUTRITION
(2022)
Article
Microbiology
Judith Van Dingenen, Sonia Garcia Mendez, Stien Beirinckx, Lena Vlaminck, Annick De Keyser, Naomi Stuer, Severine Verschaete, Alexander Clarysse, Joke Pannecoucque, Stephane Rombauts, Isabel Roldan-Ruiz, Anne Willems, Sofie Goormachtig
Summary: Soybeans used for food and feed in Europe have negative impacts due to imports, leading to the need for increased local production and the search for indigenous nodulating strains in Belgium. The study highlights the diverse bacterial richness and the necessity of combining different identification techniques to capture the true nodule rhizobial community. Genomic analysis revealed multiple genetically distinct species capable of nodulating soybeans in laboratory conditions.
ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Luna M. van der Loos, Sofie D'hondt, Aschwin H. Engelen, Henrik Pavia, Gunilla B. Toth, Anne Willems, Florian Weinberger, Olivier De Clerck, Sophie Steinhagen
Summary: This study investigated the stability and variability of seaweed-associated bacteria across the Atlantic-Baltic Sea salinity gradient. Results showed that bacterial composition was strongly influenced by salinity and host species, with distinct communities in low and high salinity regions. A small taxonomic core community was identified, contributing to 14% of reads per sample, and core taxa followed a gradient model, facilitating host adaptation across the environmental gradient.
Article
Microbiology
Luc Cornet, Ilse Cleenwerck, Jessy Praet, Raphael R. Leonard, Nicolas J. Vereecken, Denis Michez, Guy Smagghe, Denis Baurain, Peter Vandamme
Summary: Snodgrassella is a genus of Betaproteobacteria found in the gut of honeybees and bumblebees, crucial for bee health and metabolism. Phylogenomic analysis revealed at least seven Snodgrassella species with distinct evolutionary lineages and gene characteristics.
Article
Genetics & Heredity
Gleb Goussarov, Jurgen Claesen, Mohamed Mysara, Ilse Cleenwerck, Natalie Leys, Peter Vandamme, Rob Van Houdt
Summary: This study developed a novel approach called MAGISTA, which assesses the quality of metagenome-assembled genomes based on alignment-free distance distributions between contig fragments. MAGISTA achieved a 20% reduction in root-mean-square error compared to the marker gene approach when tested on publicly available mock metagenomes. Additionally, the highly complex genomic DNA mock community used in the study serves as a valuable tool for benchmarking (new) metagenome analysis methods.
ENVIRONMENTAL MICROBIOME
(2022)
Article
Biotechnology & Applied Microbiology
Roba Mohamad, Anne Willems, Antoine Le Quere, Marjorie Pervent, Maurine Bonabaud, Emeric Dubois, Jean-Claude Cleyet-Marel, Brigitte Brunel
Summary: Ten mesorhizobial strains isolated from root-nodules of Anthyllis vulneraria in southern France were classified into two novel species within the genus Mesorhizobium.
SYSTEMATIC AND APPLIED MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Henry D. Naranjo, Liesbeth Lebbe, Margo Cnockaert, Florent Lassalle, Chin Chin Too, Anne Willems
Summary: Recently, we isolated two closely related strains, LMG 31531T and LMG 31532, from soil and plant roots, respectively. Based on genomic and phenotypic data, these strains differ from previously reported species and are described as a new species, Agrobacterium divergens. Our comparative genomic study also discovered conserved proteins that provide insights into the functional evolution of this genus.
SYSTEMATIC AND APPLIED MICROBIOLOGY
(2023)
Article
Pharmacology & Pharmacy
Angelique Rat, Antigoni E. Koletti, Nebojsa Rodic, Vassilios P. Papageorgiou, Anne Willems, Andreana N. Assimopoulou
Summary: In this study, a strain of Pseudomonas sp. R-72008 was found to utilize Alkannin, shikonin, and their derivatives as the sole carbon source. This bacterial strain was able to modify the composition of A/S, decreasing the amount of A/S monomers and increasing the production of A/S oligomers. This research provides new insights into the interactions between bacteria and plant antimicrobials.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Microbiology
Henry D. Naranjo, Angelique Rat, Noemie De Zutter, Emmelie De Ridder, Liesbeth Lebbe, Kris Audenaert, Anne Willems
Summary: Phytopathogenic fungi pose a major threat to global food production, and the use of biocontrol agents derived from medicinal plants could provide an alternative to chemical fungicides. The study identified Pseudomonas sp. strain R-71838 as a promising candidate for biocontrol of fungal pathogens, based on its strong antifungal activity both in vitro and in planta.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Luna M. van der Loos, Lander De Coninck, Roland Zell, Sebastian Lequime, Anne Willems, Olivier De Clerck, Jelle Matthijnssens
Summary: Marine macroalgae (seaweeds) play a vital role in coastal ecosystems as primary producers and foundation species. However, little is known about the diversity and impact of viruses in seaweeds. This study characterized viral communities in healthy and bleached specimens of the commercially important green seaweed Ulva, revealing the presence of diverse and novel viruses. The abundance of certain viruses was significantly higher in the bleached samples, suggesting their possible role in seaweed bleaching.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Klara Filek, Liesbeth Lebbe, Anne Willems, Peter Chaerle, Wim Vyverman, Marta Zizek, Suncica Bosak
Summary: Diatoms and bacteria are the first colonizers of marine reptile skin, and sea turtle carapace and skin harbor a diverse microbial community. This study investigated the diatoms, bacteria, and diatom-associated bacteria from loggerhead sea turtles using molecular and culturing approaches. High diversity of diatoms and bacteria was found, and the bacterial communities reflected those of the host. Diatom strains allowed for isolation of rare bacterial families, suggesting the potential for novel microbes and important diatom-bacteria associations in epizoic biofilms.
FEMS MICROBIOLOGY ECOLOGY
(2022)
