Article
Biochemistry & Molecular Biology
Yuji Sasaki, Yoshiki Higashijima, Jun-Ichi Suehiro, Takehito Sugasawa, Eri Oguri-Nakamura, Shigetomo Fukuhara, Nao Nagai, Yosuke Hirakawa, Youichiro Wada, Masaomi Nangaku, Yasuharu Kanki
Summary: "Research has shown that KDM2B plays a crucial regulatory role in VEGF signaling. Knockdown of KDM2B enhances VEGF-induced angiogenesis in endothelial cells, while ectopic expression of KDM2B inhibits angiogenesis. KDM2B selectively controls the transcription levels of genes associated with VEGF-induced angiogenesis, thus affecting endothelial cell function."
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Cell Biology
Ziyu Zhang, Baoyu Chen, Yuwen Zhu, Tianyi Zhang, Yibiao Yuan, Xiaoling Zhang, Yong Xu
Summary: TGF-beta regulates the transcription of the prometastatic small GTPase RHOJ by activating MKL1 and recruiting the H3K9/H3K27 dual demethylase KDM7A. KDM7A can be used to predict prognosis in breast cancer patients and its knockdown attenuates migration, invasion, growth, and metastasis of breast cancer cells. KDM7A is a direct transcriptional target of TGF-beta signaling, and small-molecule inhibitors of KDM7A may provide therapeutic solutions for malignant breast cancers.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemical Research Methods
Matthew Hoekstra, Kyle K. Biggar
Summary: The study focuses on the role of KDM5A in regulating gene expression through histone methylation and suggests its potential involvement with non-histone proteins. By examining a library of 180 peptide substrates, a recognition motif was identified and successfully used to predict and validate high-ranking substrates. The approach demonstrated the utility in identifying candidate substrates applicable to Fe(II)-and 2-oxoglutarate dependent demethylases.
ANALYTICAL BIOCHEMISTRY
(2021)
Article
Chemistry, Medicinal
Tianyou Wang, Wanzhen Yang, Yun Liu, Wang Li, Yan Wang, Na Liu, Chunquan Sheng
Summary: Invasive fungal infections are a global health problem, and the lack of effective antifungal drugs poses a challenge for clinical treatment. However, a new study has identified JIB-04 as a potential antifungal agent with broad-spectrum activity, particularly against Cryptococcus. JIB-04 inhibits key processes in Cryptococcus cells and reduces their virulence factors. This finding offers a potential treatment option for cryptococcal meningitis and highlights Jumonji histone demethylase as a potential target for the development of novel antifungal therapeutics.
ACS INFECTIOUS DISEASES
(2022)
Article
Oncology
Yi He, Xin Yi, Zihao Zhang, Hanshen Luo, Rui Li, Xin Feng, Ze-Min Fang, Xue-Hai Zhu, Wenlin Cheng, Ding-Sheng Jiang, Fang Zhao, Xiang Wei
Summary: Jumonji demethylases, including JIB-04, are involved in the regulation of VSMC phenotype switching and neointima formation. JIB-04 inhibits VSMC proliferation, migration, and contractile phenotype by impairing autophagic flux. It has potential as a reagent for the treatment of neointima formation.
CLINICAL EPIGENETICS
(2022)
Article
Oncology
Lei Duan, Ricardo E. Perez, Sarah Calhoun, Carl G. Maki
Summary: This study established cell line and PDX tumor model systems of cisplatin and paclitaxel-resistant NSCLC to investigate the role of Jumonji C domain-containing family of histone lysine demethylases (Jumonji KDMs) in cancer therapy response and DNA repair. The results showed that inhibition of Jumonji KDMs sensitized the resistant cells and tumors to cisplatin and paclitaxel, partly by promoting APC/Cdh1-dependent degradation of CtIP and PAF15, two DNA repair proteins. These findings suggest that targeting Jumonji-KDMs could enhance the effectiveness of therapy in NSCLC.
CANCER BIOLOGY & THERAPY
(2022)
Article
Plant Sciences
Surbhi Mali, Madhushree Dutta, Komal Goel, Vidhi Raturi, Pravesh Kundu, Ravi Kumar, Vijay Gahlaut, Vishal Acharya, Vijai Kishor Gupta, Salej Sood, Gaurav Zinta
Summary: Potato is an important food crop that is rich in starch, proteins, antioxidants, and vitamins. However, it is negatively affected by temperature rise. In this study, 26 StJMJ proteins were identified in potato, and they were classified into five different classes. Gene expression analysis showed that the StJMJ genes are heat-inducible and play an important role in modulating heat tolerance in potato.
JOURNAL OF PLANT GROWTH REGULATION
(2023)
Review
Cell Biology
Jack Crouch, Maria Shvedova, Rex Jeya Rajkumar Samdavid Thanapaul, Vladimir Botchkarev, Daniel Roh
Summary: Senescence is a complex cellular stress response that leads to loss of proliferative capacity and changes in secretory pattern. Transcriptional regulation of multiple genes and epigenetic alterations to DNA and chromatin play crucial roles in the induction and maintenance of senescence. This review highlights the changes in chromatin, DNA methylation, histone alterations, and the specific epigenetic regulation of the senescence-associated secretory phenotype (SASP).
Article
Chemistry, Medicinal
Lennart Brewitz, Yu Nakashima, Sonia K. Piasecka, Eidarus Salah, Sally C. Fletcher, Anthony Tumber, Thomas P. Corner, Tristan J. Kennedy, Giorgia Fiorini, Armin Thalhammer, Kirsten E. Christensen, Mathew L. Coleman, Christopher J. Schofield
Summary: Jumonji-C domain-containing protein 5 (JMJD5) is an important 2-oxoglutarate-dependent oxygenase that has various functions in development, circadian rhythm, and cancer. This study discovered potent JMJD5 inhibitors, which are 5-aminoalkyl-substituted derivatives of the broad-spectrum 2OG oxygenase inhibitor pyridine-2,4-dicarboxylic acid (2,4-PDCA). Crystallographic analyses showed that these inhibitors bind to JMJD5 in an induced fit manner, with the C5 substituent of 2,4-PDCA binding to the substrate-binding pocket of JMJD5. Cellular studies revealed that these lead compounds displayed similar phenotypes as clinically observed JMJD5 variants with reduced catalytic activity.
JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Helena Malvezzi, Cristine Dobo, Renee Zon Filippi, Helen Mendes do Nascimento, Laura Palmieri da Silva e Sousa, Juliana Meola, Carla Azevedo Piccinato, Sergio Podgaec
Summary: The accumulation of senescent cells in endometriosis tissues is associated with an inflammatory microenvironment and the presence of pro-inflammatory markers. The expression of senescence markers varies with the phases of the menstrual cycle, and IL-1 beta levels are significantly higher in endometriosis lesions. These findings provide potential targets for therapeutic interventions to alleviate endometriosis symptoms.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Medicinal
Martina Menna, Francesco Fiorentino, Biagina Marrocco, Alessia Lucidi, Stefano Tomassi, Domenica Cilli, Mauro Romanenghi, Matteo Cassandri, Silvia Pomella, Michele Pezzella, Donatella Del Bufalo, Mohammad Salik Zeya Ansari, Nevena Tomasevic, Milan Mladenovic, Monica Viviano, Gianluca Sbardella, Rossella Rota, Daniela Trisciuoglio, Saverio Minucci, Andrea Mattevi, Dante Rotili, Antonello Mai
Summary: Chemical modifications of LSD1 led to highly active and selective inhibitors, which showed antiproliferative effects and gene expression regulation in leukemia cells. The inhibition of LSD1 demonstrated a crucial role in solid tumor cells, suggesting no added value for simultaneous G9a inhibition.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Chemistry, Medicinal
David M. Carter, Edgar Specker, Piotr H. Malecki, Jessica Przygodda, Krystyna Dudaniec, Manfred S. Weiss, Udo Heinemann, Marc Nazare, Ulrich Gohlke
Summary: A benzyl-substituted variant of an inhibitor targeting KDM4 enzymes demonstrates improved potency, cell-permeability, and cytotoxicity against prostate cancer cells. The inhibition mechanism involves competition with Fe2+ at the active site and binding to a distal site on the enzyme surface. The inhibitor's cytostatic effect is attributed to direct intracellular inhibition of KDM4 enzymes, leading to altered gene expression and epigenetic changes in PCa cells.
JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Review
Biochemistry & Molecular Biology
Thi Hang Giang Phan, Panagiotis Paliogiannis, Gheyath K. Nasrallah, Roberta Giordo, Ali Hussein Eid, Alessandro Giuseppe Fois, Angelo Zinellu, Arduino Aleksander Mangoni, Gianfranco Pintus
Summary: Idiopathic pulmonary fibrosis (IPF) is a progressive, irreversible, and typically lethal lung disease with poorly understood mechanisms, involving various cellular processes and molecular pathways. Apoptosis, senescence, epithelial-mesenchymal transition, and other processes have been identified as key factors in tissue remodeling associated with IPF.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Jeet Maity, Satyabrata Majumder, Ranjana Pal, Bhaskar Saha, Prabir Kumar Mukhopadhyay
Summary: Ascorbic acid functions as a redox regulator and has various effects on the expression and differentiation of immune cells, through its interaction with JHDM and TET.
Review
Medicine, Research & Experimental
Shan Han, Qiangwei Lu, Xiaoqiu Liu
Summary: Idiopathic pulmonary fibrosis (IPF) is a progressive, irreversible and fatal interstitial lung disease of unknown cause, with a median survival of 2-3 years. Aging may be linked to the development of IPF, but the mechanism remains unclear. Senescence of alveolar epithelial cells and lung fibroblasts (LFs) and their senescence-associated secretion phenotype (SASP) may play a role in IPF. This review focuses on the senescence of LFs and epithelial and stem cells, SASP, profibrotic signaling pathway activation, and potential treatments for IPF pathogenesis.
EXPERIMENTAL AND THERAPEUTIC MEDICINE
(2023)
Article
Microbiology
Erika Wallender, Nan Zhang, Melissa Conrad, Abel Kakuru, Mary Muhindo, Patrick Tumwebaze, Richard Kajubi, Daniel Mota, Jennifer Legac, Prasanna Jagannathan, Diane Havlir, Moses Kamya, Grant Dorsey, Francesca Aweeka, Philip J. Rosenthal, Rada M. Savic
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2019)
Article
Oncology
Zi-Ming Zhao, Susan E. Yost, Katherine E. Hutchinson, Sierra Min Li, Yate-Ching Yuan, Javad Noorbakhsh, Zheng Liu, Charles Warden, Radia M. Johnson, Xiwei Wu, Jeffrey H. Chuang, Yuan Yuan
Editorial Material
Oncology
Xia Gao, Jason W. Locasale, Michael A. Reid
Article
Toxicology
Noriko Kanaya, Lauren Bernal, Gregory Chang, Takuro Yamamoto, Duc Nguyen, Yuan-Zhong Wang, June-Soo Park, Charles Warden, Jinhui Wang, Xiwei Wu, Timothy Synold, Michele Rakoff, Susan L. Neuhausen, Shiuan Chen
TOXICOLOGICAL SCIENCES
(2019)
Article
Multidisciplinary Sciences
Ying Yang, Mari B. Ishak Gabra, Eric A. Hanse, Xazmin H. Lowman, Thai Q. Tran, Haiqing Li, Neta Milman, Juan Liu, Michael A. Reid, Jason W. Locasale, Ziv Gil, Mei Kong
NATURE COMMUNICATIONS
(2019)
Article
Cell Biology
Jennifer Yinuo Cao, Aunoy Poddar, Leslie Magtanong, Jennifer H. Lumb, Trevor R. Mileur, Michael A. Reid, Cole M. Dovey, Jin Wang, Jason W. Locasale, Everett Stone, Susan P. C. Cole, Jan E. Carette, Scott J. Dixon
Correction
Biochemistry & Molecular Biology
Charles D. Warden, Heehyoung Lee, Joshua D. Tompkins, Xiaojin Li, Charles Wang, Arthur D. Riggs, Hua Yu, Richard Jove, Yate-Ching Yuan
NUCLEIC ACIDS RESEARCH
(2019)
Correction
Biotechnology & Applied Microbiology
Charles Warden, Qiyi Tang, Hua Zhu
BIOMED RESEARCH INTERNATIONAL
(2019)
Article
Cell Biology
Anna-Maria Barciszewska, Malgorzata Giel-Pietraszuk, Patrick M. Perrigue, Miroslawa Naskret-Barciszewska
Article
Virology
Lorraine Z. Mutsvunguma, Esther Rodriguez, Gabriela M. Escalante, Murali Muniraju, John C. Williams, Charles Warden, Hanjun Qin, Jinhui Wang, Xiwei Wu, Anne Barasa, David H. Mulama, Waithaka Mwangi, Javier Gordon Ogembo
Article
Oncology
Patrick M. Perrigue, Magdalena Rakoczy, Kamila P. Pawlicka, Agnieszka Belter, Malgorzata Giel-Pietraszuk, Miroslawa Naskret-Barciszewska, Jan Barciszewski, Marek Figlerowicz
Article
Biochemistry & Molecular Biology
Eliza Wyszko, Mariusz Popenda, Dorota Gudanis, Agnieszka Belter, Patrick Perrigue, Katarzyna Rolle, Jan Barciszewski, Joanna Sarzynska, Pawel Skowronek
Summary: Researchers have found that replacing RNA's D-ribose backbone with L-ribose isomers can increase its resistance to nucleases and improve its chances of survival in cells. They investigated the activity of left-handed hammerhead ribozyme and demonstrated the ability of L-ribose to reverse the chirality of RNA.
BIOSCIENCE REPORTS
(2021)
Article
Biology
Zhiqiang Wang, Chunxiao Zhang, Charles David Warden, Zheng Liu, Yate-Ching Yuan, Chao Guo, Charles Wang, Jinhui Wang, Xiwei Wu, Richard Ermel, Steven L. Vonderfecht, Xiuli Wang, Christine Brown, Stephen Forman, Yaling Yang, M. James You, WenYong Chen
Summary: SIRT1 plays an unexpected role in promoting aging of HSCs and age-dependent MPAL. Knockout of Sirt1 improves the function of aging HSCs and suppresses the development of MPAL.
COMMUNICATIONS BIOLOGY
(2022)
Article
Genetics & Heredity
Ronald M. Goto, Charles D. Warden, Takashi Shiina, Kazuyoshi Hosomichi, Jibin Zhang, Tae Hyuk Kang, Xiwei Wu, Marla C. Glass, Mary E. Delany, Marcia M. Miller
Summary: MHCY is a significant histocompatibility complex-like gene region in chickens, with a high density of repetitive sequences and multiple gene families. Through sequencing analysis, it was found that MHCY region includes various genes such as class I-like genes, c-type-lectin-like genes, etc., characterized by polymorphism and nested within retroelements.
G3-GENES GENOMES GENETICS
(2022)
Article
Biology
Noriko Kanaya, Gregory Chang, Xiwei Wu, Kohei Saeki, Lauren Bernal, Hyun-Jeong Shim, Jinhui Wang, Charles Warden, Takuro Yamamoto, Jay Li, June-Soo Park, Timothy Synold, Steve Vonderfecht, Michele Rakoff, Susan L. Neuhausen, Shiuan Chen
COMMUNICATIONS BIOLOGY
(2019)