4.6 Article

Mapping and allelic variations of Ppo-B1 and Ppo-B2 gene-related polyphenol oxidase activity in durum wheat

Journal

MOLECULAR BREEDING
Volume 35, Issue 2, Pages -

Publisher

SPRINGER
DOI: 10.1007/s11032-015-0272-y

Keywords

Durum wheat; Polyphenol oxidase; Ppo-B1; Ppo-B2; Marker-assisted selection

Funding

  1. MiUR Project PON-ISCOCEM
  2. MiSE Project PAQ-Industria

Ask authors/readers for more resources

Polyphenol oxidase (PPO) activity is one of the major causes of undesirable brown color of grain and flour of durum and common wheat. The Ppo-A1, Ppo-A2, Ppo-D1 and Ppo-D2 genes are known to be involved in PPO activity, while the role of Ppo-B1 and Ppo-B2 genes is not accurately established. The goals of this study were to develop homoeologspecific markers to determine the genetic map position of Ppo-B1 and Ppo-B2, detect new allelic variants and to verify their association with PPO activity. Two new functional markers, MG08 and MG33, were developed for Ppo-B1 and Ppo-B2, respectively, and mapped on chromosome bin C-2BL6-0.88, separated by 11.4 cM. Screening of a tetraploid wheat collection with MG08 and MG33 identified four and two alleles, respectively, including three novel alleles of Ppo-B1 (Ppo-B1b, PpoB1c and Ppo-B1d) and one novel allele of Ppo-B2 (Ppo-B2d). Ppo-B1 and Ppo-B2 were significantly associated with PPO activity evaluated in replicated trials carried out in 3 years. The newly developed homoeolog-specific markers can be used in wheat breeding to improve the quality of wheat-based products.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.6
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

Article Food Science & Technology

Extra Virgin Olive Oils with High Phenolic Content as an Ingredient of Artisanal Ice Cream: Consumer Acceptance

Roberta Monteiro Caldeira, Roberta Fontanive Miyahira, Camila Faria Lima, Carolyne Pimentel Rosado, Ana Claudia Campos, Renata Rangel Guimaraes, Nathalia Moura Nunes, Graziana Difonzo, Giacomo Squeo, Antonella Pasqualone, Francesco Caponio, Rafael Da Silva Cadena, Lilia Zago

Summary: This study aimed to evaluate the acceptance of artisanal ice cream prepared with extra virgin olive oil (EVOO) as the main lipid ingredient. The results showed that there were no significant differences in taste, appearance, and overall acceptance between the ice creams made with EVOOs from different regions, and the overall acceptance mainly correlated with aroma and flavor.

JOURNAL OF CULINARY SCIENCE & TECHNOLOGY (2023)

Article Agriculture, Multidisciplinary

Almond okara as a valuable ingredient in biscuit preparation

Davide De Angelis, Antonella Pasqualone, Giacomo Squeo, Carmine Summo

Summary: Almond okara, a valuable by-product, can be used in biscuit preparation to improve nutritional value with limited impact on sensory properties.

JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE (2023)

Article Food Science & Technology

Milling performance and bread-making aptitude of the new soft kernel durum wheat variety Faridur

Antonella Pasqualone, Samuela Palombieri, Hamit Koksel, Carmine Summo, Pasquale De Vita, Francesco Sestili

Summary: In this study, the milling performance and bread-making ability of Faridur, the first 'soft kernel' durum wheat variety released in Italy, were compared with Svevo. Faridur showed higher protein and gluten content, but lower gluten quality. Its softer nature facilitated energy-saving milling, but adjustments were needed for baking processes and formulations.

INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY (2023)

Review Plant Sciences

Genetic approaches to exploit landraces for improvement of Triticum turgidum ssp. durum in the age of climate change

Chiara Broccanello, Diana Bellin, Giovanni DalCorso, Antonella Furini, Francesca Taranto

Summary: Addressing climate change challenges in durum wheat production is crucial for ensuring food and nutrition security in the Mediterranean region. Exploiting the genetic variability in landrace populations has emerged as a strategy to enhance durum wheat adaptation to environmental stresses. This review highlights the importance of durum wheat landraces as a genetic resource for improving the sustainability of Mediterranean agroecosystems, focusing on adaptive traits and the development of molecular markers for selection.

FRONTIERS IN PLANT SCIENCE (2023)

Article Food Science & Technology

Effect of Oil Type Used in Neapolitan Pizza TSG Topping on Its Physical, Chemical, and Sensory Properties

Amalia Piscopo, Angela Zappia, Antonio Mincione, Roccangelo Silletti, Carmine Summo, Antonella Pasqualone

Summary: According to the regulations of the Neapolitan Pizza TSG, extra virgin olive oil must be exclusively used as a topping ingredient, together with tomato for pizza marinara-type production. The present study was conducted to analyze the quality of pizza depending on the oil typology used, revealing that monovarietal olive oil is the best choice due to its higher quality and better health characteristics.

FOODS (2023)

Article Agriculture, Multidisciplinary

Varietal identification in pasta through an SSR-based approach: a case study

Valentina Fanelli, Maria Dellino, Francesca Taranto, Claudio De Giovanni, Wilma Sabetta, Pasquale De Vita, Cinzia Montemurro

Summary: In this study, a simple sequence repeats-based method was used to identify durum wheat varieties used in the production of 25 samples of semolina and commercial pasta. The molecular profiles of these samples were compared with those of declared varieties and other commonly used durum wheat cultivars. The method successfully detected not declared varieties present in a percentage equal to or higher than 5%.

JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE (2023)

Article Food Science & Technology

Effect of dry-fractionated pea protein on the physicochemical properties and the nutritional features of gluten-free focaccia flat bread

Davide De Angelis, Francesca Vurro, Maria Santamaria, Raquel Garzon, Cristina M. Rosell, Carmine Summo, Antonella Pasqualone

Summary: The aim of this study was to develop a gluten-free focaccia flat bread using rice and corn flour fortified with dry-fractionated pea protein concentrate. The flour ratios significantly influenced the physical and sensory properties of the dough and breads. The optimal formulation contained 5 g/100 g of pea protein concentrate, 20 g/100 g of rice flour and corn flour each, resulting in a bread with desirable characteristics.

LWT-FOOD SCIENCE AND TECHNOLOGY (2023)

Article Food Science & Technology

Screening of Acrylamide Content in Commercial Plant-Based Protein Ingredients from Different Technologies

Giacomo Squeo, Davide De Angelis, Antonio Francesco Caputi, Antonella Pasqualone, Carmine Summo, Francesco Caponio

Summary: The demand for plant-based protein ingredients (PBPIs) in the food sector has increased significantly. This study evaluated the acrylamide contamination in PBPIs produced with different technologies. The results showed a significant variability in acrylamide contamination among different PBPI categories, with flour having the lowest average acrylamide content and wet-extracted proteins having the highest. These findings emphasize the importance of monitoring acrylamide formation during PBPI processing and developing mitigation strategies when necessary.

FOODS (2023)

Article Plant Sciences

SNP Diversity and Genetic Structure of Rogosija, an Old Western Balkan Durum Wheat Collection

Ana Velimirovic, Zoran Jovovic, Dragan Perovic, Heike Lehnert, Sanja Mikic, Dragan Mandic, Novo Przulj, Giacomo Mangini, Mariella Matilde Finetti-Sialer

Summary: Durum wheat landraces, such as Rogosija, are valuable genetic resources for identifying and isolating new genes and alleles that can enhance crop adaptability to climate change. In this study, the genetic diversity of the Rogosija collection was estimated using morphological descriptors and SNP array. The analysis revealed two distinct clusters, indicating the presence of two different Balkan durum landrace collections evolved in different eco-geographic micro-areas. The origins of Balkan durum landraces were also discussed.

PLANTS-BASEL (2023)

Article Food Science & Technology

Nutritional features of flour blends composed of durum wheat and lupin

Alfio Spina, Michele Scarangella, Michele Canale, Rosalia Sanfilippo, Virgilio Giannone, Carmine Summo, Antonella Pasqualone

Summary: This study aimed to improve the nutritional characteristics of durum wheat re-milled semolina by adding lupin flour and lupin protein concentrate. The addition of lupin flour and lupin protein concentrate significantly increased the protein content and lysine score of the flour blends. The macroelements, such as magnesium, potassium, calcium, and phosphorous, also significantly increased with the addition of lupin flour and lupin protein concentrate.

INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY (2023)

Article Agriculture, Multidisciplinary

Dry-fractionated protein concentrate as egg replacer in sponge cake: how the rheological properties of the batters affect the physical and structural quality of the products

Davide De Angelis, Vittoria Latrofa, Giacomo Squeo, Antonella Pasqualone, Carmine Summo

Summary: Dry-fractionated protein concentrates (DFp) can effectively replace eggs in cakes and impact the rheological properties of the batter. Higher DFp content results in harder cakes but emulates the behavior of eggs.

JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE (2023)

Review Plant Sciences

Genomics for Yield and Yield Components in Durum Wheat

Francesca Taranto, Salvatore Esposito, Pasquale De Vita

Summary: In recent years, the genetic basis of yield and yield components in durum wheat has been studied extensively using linkage mapping and genome-wide association studies. The reductionist approach of dissecting yield into individual components appears insufficient for significant yield increases due to limited resources, land loss, and climate change. Polyploidy in durum wheat makes it challenging to identify genes and chromosomal regions controlling grain yield. Next-generation sequencing, improved wheat genome assembly, high-throughput genotyping platforms, and genome editing technology offer great potential for revolutionizing plant breeding. Genomic selection can model optimal allelic combinations to maximize phenotypic potential under specific environments.

PLANTS-BASEL (2023)

Article Food Science & Technology

Rheological properties of dry-fractionated mung bean protein and structural, textural, and rheological evaluation of meat analogues produced by high-moisture extrusion cooking

Davide De Angelis, Christina Opaluwa, Antonella Pasqualone, Heike P. Karbstein, Carmine Summo

Summary: A closed cavity rheometer was used to investigate the rheology of dry-fractionated mung bean protein (DFMB) and the impact of high-moisture extrusion cooking on the structural, textural, and rheological properties of extrudates. The results showed that temperature and moisture content had a significant influence on the texture and rheological properties of the extrudates.

CURRENT RESEARCH IN FOOD SCIENCE (2023)

Article Chemistry, Applied

Phenolic profile of micro- and nano-encapsulated olive leaf extract in biscuits during in vitro gastrointestinal digestion

Calina Ciont, Graziana Difonzo, Antonella Pasqualone, Maria Simona Chis, Florica Ranga, Katalin Szabo, Elemer Simon, Anca Naghiu, Lucian Barbu-Tudoran, Francesco Caponio, Oana Lelia Pop, Dan Cristian Vodnar

Summary: Olive leaf extract, which is rich in phenols and flavonoids, shows functional and health-related properties. Micro- and nano-encapsulation techniques improve the stability of phenols during digestion and enhance biscuit functionality. However, high concentration adversely affects sensory and textural parameters.

FOOD CHEMISTRY (2023)

Review Agriculture, Multidisciplinary

Techno-functional properties of dry-fractionated plant-based proteins and application in food product development: a review

Davide De Angelis, Vittoria Latrofa, Francesco Caponio, Antonella Pasqualone, Carmine Summo

Summary: Dry-fractionated protein concentrates have versatile and sustainable technology, making them suitable for a wide range of plant materials. Their techno-functional properties, including water absorption, emulsification, foaming, and gelling abilities, are influenced by extraction technology and protein characteristics. These proteins have high solubility, foaming ability, foam stability, and gelling ability, which have been utilized in the development of bakery products, pasta, meat analogues, and dairy analogues. However, further research is needed to explore their potential with different plant materials. This review provides an initial overview of the techno-functional properties of dry-fractionated proteins, facilitating their application in food products.

JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE (2023)

No Data Available