Journal
INTERNATIONAL JOURNAL OF PEPTIDE RESEARCH AND THERAPEUTICS
Volume 14, Issue 4, Pages 351-357Publisher
SPRINGER
DOI: 10.1007/s10989-008-9161-0
Keywords
Protein chip; Protein trans-splicing; Split intein; Native chemical ligation; Expressed protein ligation
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Many experimental approaches in biology and biophysics, as well as applications in diagnosis and drug discovery, require proteins to be immobilized on solid supports. Protein microarrays, for example, provide a high-throughput format to study biomolecular interactions. The technique employed for protein immobilization is a key to the success of these applications. Recent biochemical developments are allowing, for the first time, the selective and traceless immobilization of proteins generated by cell-free systems without the need for purification and/or reconcentration prior to the immobilization step.
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