Review
Pharmacology & Pharmacy
Edward J. Calabrese
Summary: This paper provides a detailed assessment of hormetic dose responses in adipose-derived stem cells (ADSCs) of animal models and humans, showing that hormetic dose responses are commonly reported for ADSCs and may have a determinant role in potential clinical applications.
PHARMACOLOGICAL RESEARCH
(2021)
Review
Pharmacology & Pharmacy
Tihomir Georgiev-Hristov, Mariano Garcia-Arranz, Jacobo Trebol-Lopez, Paula Barba-Recreo, Damian Garcia-Olmo
Summary: Adipose-derived stem cells have clinically beneficial qualities and have been investigated in basic research and clinical studies. Allogeneic adipose-derived stem cells have recently been approved for treating complex perianal fistulas in adults with Crohn's disease. The current understanding of adipose stem cell harvest includes quantitative and qualitative differences that may be influenced by donor conditions and sites. This review summarizes the current knowledge on the topic and proposes optimal donor profiles for further applications and studies.
Article
Chemistry, Physical
Chunhui Sheng, Xiaodong Guo, Zhuqing Wan, Xiaoqiang Bai, Hao Liu, Xiao Zhang, Ping Zhang, Yunsong Liu, Wenyue Li, Yongsheng Zhou, Longwei Lv
Summary: This study investigated the potential of exosomes derived from human adipose-derived stem cells (hASCs) in promoting osteogenic differentiation for osteoporosis treatment. The study identified the key differentially expressed microRNA between two exosomes and their potential target gene. Results showed that exosomes from osteogenic induction medium had a better effect on promoting osteogenic differentiation than those from proliferation medium. Moreover, miR-335-3p inhibitor-optimized exosomes enhanced osteogenic differentiation in vitro and bone density in vivo, suggesting the potential of engineered exosomes in optimizing exosome-based therapy for osteoporosis.
Article
Biotechnology & Applied Microbiology
Luminita Labusca, Dumitru-Daniel Herea, Anca Emanuela Minuti, Cristina Stavila, Camelia Danceanu, Petru Plamadeala, Horia Chiriac, Nicoleta Lupu
Summary: Iron oxide based magnetic nanoparticles (MNP) have versatile applications in biology and medicine. This study compared the chondrogenic conversion of ADSC-MNP and WJMSC under different conditions, finding that ADSC-MNP displayed superior proliferation and chondrogenic potential, with MF exposure further enhancing chondrogenesis in ADSC-MNP. Loading ADSC with MNP may be a promising approach for cartilage engineering in the future.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Cell Biology
Xiaoyun Wu, Yongxu Mu, Jingyi Yao, Fuhong Lin, Daocheng Wu, Zhijie Ma
Summary: The study revealed that ADSCs from UC patients exhibited defective immunosuppression, indicating their unsuitability for autologous transplantation in treating UC.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Anna Trzyna, Agnieszka Banas-Zabczyk
Summary: The secretome of ASCs, including exosomes, contains a variety of cytokines and non-coding RNAs that have therapeutic potential in a wide range of diseases. Utilizing ASC secretome as a cell-free therapy shows promise in overcoming limitations of cell-based therapies and improving treatment efficacy.
Article
Medicine, Research & Experimental
Francesca Toia, Elena Lo Presti, Anna Barbara Di Stefano, Marta Di Simone, Marco Trapani, Anna Maria Corsale, Carmela Picone, Francesco Moschella, Francesco Dieli, Adriana Cordova, Serena Meraviglia
Summary: This study analysed the immunomodulatory properties of spheroids from adipose stem cells (SASCs) and compared them to adipose stem cells (ADSCs). SASCs showed stronger immunomodulatory abilities in heterologous experimental settings compared to ADSCs. SASCs also demonstrated greater anti-inflammatory properties.
Article
Biochemistry & Molecular Biology
Mengzhu Lv, Simeng Zhang, Bo Jiang, Sunrun Cao, Yuqing Dong, Liu Cao, Shu Guo
Summary: The study demonstrated that adipose-derived stem cell therapy can delay replicative senescence and shift metabolic homeostasis, ultimately delaying the aging process by promoting mitophagy and improving mitochondrial quality in cells. The research also showed that ADSC therapy can improve aging-related symptoms and phenotypes through mitochondrial quality control, offering significant potential for future applications in aging-related diseases.
Article
Biotechnology & Applied Microbiology
Ziwan Ji, Zhongming Cai, Shuming Gu, Yucang He, Zikai Zhang, Tian Li, Qing Wei, Jingping Wang, Chen Ke, Liqun Li
Summary: The study found that adipose-derived exosomes (ADEs) can inhibit adipogenic differentiation of adipose-derived stem cells by activating the Hedgehog (Hh) signaling pathway, thereby reducing the adverse effects of obesity on wound healing.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Gen Li, Yin Zhang, Jiezhou Wu, Renhao Yang, Qi Sun, Yidong Xu, Bo Wang, Ming Cai, Yang Xu, Chengyu Zhuang, Lei Wang
Summary: This study explores the effects and mechanism of adipose stem cells-derived exosomes (ADSCs-Exos) and ADSCs-Exos modified tissue engineering scaffold on bone defect repair. The results demonstrate that ADSCs-Exos promote the proliferation, migration, and osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs), and ADSCs-Exos modified scaffold enhances the repair of BMSCs and bone defects both in vitro and in vivo.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Multidisciplinary Sciences
Maumita Bhattacharjee, Jorge L. Escobar Ivirico, Ho-Man Kan, Shiv Shah, Takayoshi Otsuka, Rosalie Bordett, Mohammed Barajaa, Naveen Nagiah, Rishikesh Pandey, Lakshmi S. Nair, Cato T. Laurencin
Summary: Current treatment strategies for osteoarthritis (OA) mainly focus on symptom management and have limited disease-modifying effects. However, there is growing interest in using adipose-derived stem cells (ADSCs) and biomimetic injectable hydrogels as cell delivery systems for OA treatment. In this study, an amnion membrane (AM) hydrogel was developed as a biomimetic injectable hydrogel that can self-assemble and retain stem cells at the target site. The study demonstrated that both the AM hydrogel and ADSCs possess anti-inflammatory and chondroprotective properties, and their combined use can promote cartilage tissue regeneration in a rat OA model.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Cell & Tissue Engineering
Junling Gao, Juntao Yuan, Qun Liu, Yuanli Wang, Huiwen Wang, Yingjie Chen, Wenjun Ding, Guangju Ji, Zhongbing Lu
Summary: The research found that ADSCs can protect against the adverse health effects of PM2.5 exposure by attenuating pulmonary inflammation and cell death. This suggests that ADSC transplantation may be a potential therapeutic approach for severe air pollution-associated diseases.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Cell & Tissue Engineering
Tao Wang, Yingyu Li, Yu Zhu, Zebiao Liu, Li Huang, Hongxia Zhao, Zuping Zhou, Qiong Wu
Summary: This study reveals the anti-aging and anti-obesity effects of adipose-derived stem cells (ASCs) in aged animals, while the underlying molecular mechanism remains unknown. The transplantation effects of ASCs from different age donors on natural aging and leptin knockout mice were evaluated, and the multi-omics expression profiles of ASCs from young and aged mouse donors were analyzed. It was found that ASCs from young donors induced weight and abdominal fat loss in older recipients but not in young or leptin knockout mice, and young and aged mouse donor ASCs displayed significant phenotypic differences contributing to the distinguished effects in aged mice.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Biochemistry & Molecular Biology
Maurycy Jankowski, Mariusz Kaczmarek, Grzegorz Wasiatycz, Claudia Dompe, Paul Mozdziak, Jedrzej M. Jaskowski, Hanna Piotrowska-Kempisty, Bartosz Kempisty
Summary: Next-generation sequencing analysis of gene expression changes during long-term in vitro culture and osteogenic differentiation of ASCs provided valuable insights into the molecular mechanisms involved, with the detection of numerous differentially expressed gene transcripts. Bioinformatical analysis revealed unique gene expression patterns during long-term culture and differentiation, offering important clues for the therapeutic application of stem cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell & Tissue Engineering
Yang Zhou, Bo Zhao, Xin-Liao Zhang, Yi-jun Lu, Shou-Tao Lu, Jian Cheng, Yu Fu, Lin Lin, Ning-Yan Zhang, Pei-Xin Li, Jing Zhang, Jun Zhang
Summary: This study investigated the potential clinical application roles of human adipose-derived mesenchymal stem cells (hADSC) and hADSC-derived exosomes (hADSC-Exo) in cutaneous wound healing. The combined administration of local hADSC-Exo smearing and hADSC/hADSC-Exo intravenous administration showed additional benefits in promoting wound healing, accelerating re-epithelialization, reducing scar widths, and enhancing angiogenesis and collagen synthesis.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Biochemistry & Molecular Biology
Satoshi Yamada, Yoshiaki Suzuki, Eiva Bernotiene, Wayne R. Giles, Yuji Imaizumi, Hisao Yamamura
Summary: The study revealed that the swelling-activated Cl(-1) currents and regulatory volume decrease in human articular chondrocytes are regulated by CIC-3, and can be attenuated by DIDS and CIC-3 knockdown. Hypoosmotic stimulation triggers Ca2+ influx and PGE(2) release in OUMS-27 cells, which are reduced by DIDS and CIC-3 knockdown, suggesting an association of CIC-3 with cartilage degenerative diseases.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Review
Biotechnology & Applied Microbiology
Ilona Uzieliene, Ursule Kalvaityte, Eiva Bernotiene, Ali Mobasheri
Summary: Gene therapy has been explored as a potential treatment for osteoarthritis, with a focus on non-viral gene delivery technologies. While viral gene delivery has been more popular due to higher efficiency, efforts to enhance transfection efficiency of non-viral methods have shown promise for OA research and clinical applications. More research is needed to optimize transfection efficiency and duration of gene expression.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Multidisciplinary Sciences
Raimonda Kubiliute, Indre Januskeviciene, Ruta Urbanaviciute, Kristina Daniunaite, Monika Drobniene, Valerijus Ostapenko, Rimantas Daugelavicius, Sonata Jarmalaite
Summary: The hyperactivation of ABC transporter ABCB1 and the induction of epithelial-mesenchymal transition (EMT) are common mechanisms of acquired cancer chemoresistance. This study investigates the mechanisms contributing to upregulation of ABCB1 and the acquisition of doxorubicin (DOX) resistance in breast cancer MX-1 cell line, suggesting that non-genotoxic ABCB1 inducers may accelerate the development of DOX resistance.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Kristina Daniunaite, Arnas Bakavicius, Kristina Zukauskaite, Ieva Rauluseviciute, Juozas Rimantas Lazutka, Albertas Ulys, Feliksas Jankevicius, Sonata Jarmalaite
Summary: This study identified methylation of ADAMTS12, NAALAD2, and PRKCB as novel diagnostic and prognostic markers for prostate cancer, showing independent predictive value for biochemical disease recurrence and improved prognostic power in multivariate models.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Immunology
Regina Sakalyte, Jaroslav Denkovskij, Eiva Bernotiene, Sigita Stropuviene, Silvija Ona Mikulenaite, Giedrius Kvederas, Narunas Porvaneckas, Vytautas Tutkus, Algirdas Venalis, Irena Butrimiene
Summary: This study investigated the expression and secretion of activated rheumatoid arthritis synovial fibroblasts and their correlation with rheumatoid arthritis and other forms of arthritis. The results showed that NLRP3 was associated with inflammatory activation, and vitamin D may have a protective effect on inflammatory responses. Inflammatory responses may also be age-related.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Orthopedics
Ursule Kalvaityte, Csaba Matta, Eiva Bernotiene, Peter Natesan Pushparaj, Ata M. Kiapour, Ali Mobasheri
Summary: This article reviews the biological roles of clusterin in cartilage, chondrocytes, and other synovial joint tissues, and discusses its potential as a biomarker in synovial and systemic fluids of patients with rheumatoid arthritis (RA) or osteoarthritis (OA). Clusterin has cytoprotective functions and may serve as a biomarker of early repair responses in OA.
JOURNAL OF ORTHOPAEDIC TRANSLATION
(2022)
Article
Chemistry, Analytical
Almira Ramanaviciene, Anton Popov, Ema Baliunaite, Benediktas Brasiunas, Asta Kausaite-Minkstimiene, Ugur Tamer, Gailute Kirdaite, Eiva Bernotiene, Ali Mobasheri
Summary: This study presents the development of a specific magnetic immunoassay method for the determination of hGH concentration in serum. The method has a low limit of detection, clinically relevant linear range, and negligible non-specific binding. Important technical solutions for the development of this sandwich-type colorimetric magneto-immunoassay are discussed.
Article
Biochemistry & Molecular Biology
Anton Popov, Viktorija Lisyte, Asta Kausaite-Minkstimiene, Eiva Bernotiene, Almira Ramanaviciene
Summary: A method using surface plasmon resonance (SPR) spectroscopy for evaluating the conjugation efficiency of antibody-functionalized quantum dots (QDs) with specific antigens was proposed. Experimental results showed that the optimal molar ratio of QDs-NH2 to anti-CD44 for conjugation was found to be 1:10, based on the comparable interaction between the conjugates and the immobilized CD44 biomarkers.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Ieva Sadzeviciene, Kristina Snipaitiene, Asta Scesnaite-Jerdiakova, Kristina Daniunaite, Rasa Sabaliauskaite, Aida Laurinaviciene, Monika Drobniene, Valerijus Ostapenko, Sonata Jarmalaite
Summary: This study analyzed the DNA methylation pattern and TP53 mutation status of intrinsic breast cancer subtypes and found that specific gene methylation patterns can effectively separate breast cancer from noncancerous breast tissues, and distinguish triple-negative breast cancer from non-triple-negative breast cancer. Additionally, combinations of two-to-three methylated biomarkers can predict the prognosis of breast cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Ilona Uzieliene, Daiva Bironaite, Edvardas Bagdonas, Jolita Pachaleva, Arkadij Sobolev, Wei-Bor Tsai, Giedrius Kvederas, Eiva Bernotiene
Summary: This study investigated the chondrogenic responses of human bone marrow mesenchymal stem cells (BMMSCs) and osteoarthritis (OA) cartilage-derived chondrocytes in 3D hydrogels under mechanical load conditions. The results showed that chondrocytes had slower proliferation rate, smaller size, and higher intracellular calcium level compared to BMMSCs. Chondrocytes secreted more cartilage oligomeric matrix protein (COMP) and expressed collagen type II (COL2A1) and aggrecan (ACAN) genes but were more susceptible to mechanical load compared to BMMSCs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Ilona Uzieliene, Daiva Bironaite, Jolita Pachaleva, Edvardas Bagdonas, Arkadij Sobolev, Wei-Bor Tsai, Giedrius Kvedaras, Eiva Bernotiene
Summary: This study investigated the effect of mechanical load on the chondrogenic differentiation of bone marrow mesenchymal stem cells (BM-MCSs) encapsulated into cartilage tissue extracellular matrix (ECM). The results showed that mild mechanical load stimulated the chondrogenic differentiation of BM-MSCs, while stronger mechanical load had negative effects on human OA cartilage explants. The CS-Tyr/Gel/BM-MSCs composite reduced the release of cartilage degradation components from the OA cartilage explants.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Ilona Uzieliene, Daiva Bironaite, Rokas Miksiunas, Edvardas Bagdonas, Raminta Vaiciuleviciute, Ali Mobasheri, Eiva Bernotiene
Summary: This study investigated the role of intracellular calcium (iCa(2+)) and its regulation through voltage-operated calcium channels (VOCC) in the chondrogenic differentiation of mesenchymal stem/stromal cells. Results showed that chondrocytes had the highest level of iCa(2+), while MenSCs had the largest iCa(2+) store capacity and proliferation ability. The expression of the CaV1.2 gene was highest in MenSCs during chondrogenic differentiation. Nifedipine, a CaV1.2 antagonist, could suppress iCa(2+) and proliferation in all cells and affect iCa(2+) stores, especially in BMMSCs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biotechnology & Applied Microbiology
Raminta Vaiciuleviciute, Ilona Uzieliene, Paulius Bernotas, Vitalij Novickij, Aidas Alaburda, Eiva Bernotiene
Summary: Electrical stimulation (ES) has shown positive effects on cellular functions and chondrogenic differentiation. This review focuses on the application of ES for chondrocyte and mesenchymal stem cell chondrogenesis to promote cartilage tissue regeneration. Different types of ES and their advantageous effects are summarized, along with recommendations for reporting on ES use in studies. This review provides novel insights for in vitro studies and future cartilage repair techniques.
BIOENGINEERING-BASEL
(2023)
Article
Biotechnology & Applied Microbiology
Oleg Ardatov, Viktorija Aleksiuk, Algirdas Maknickas, Rimantas Stonkus, Ilona Uzieliene, Raminta Vaiciuleviciute, Jolita Pachaleva, Giedrius Kvederas, Eiva Bernotiene
Summary: The aim of this study was to investigate the stressed state of cartilage using different models of meniscal tear. An anatomical model of the knee joint was developed and the nonlinear mechanical properties of cartilage and meniscus were verified. The stress-strain curve of the meniscus was obtained through compression testing of human meniscus tissue specimens. The results showed that a more deteriorated meniscus had greater stiffness, but its integrity had the greatest impact on cartilage stress growth. Cases of radial, longitudinal, and complex tears were examined to confirm this. The study's methodology and results can aid in medical diagnostics for meniscus treatment and replacement.
BIOENGINEERING-BASEL
(2023)
Article
Immunology
Moritz J. Strowitzki, Ross Nelson, Mario P. Garcia, Christopher Tuffs, Marc B. Bleul, Stephen Fitzsimons, Javier Navas, Ilona Uzieliene, Alina S. Ritter, David Phelan, Sarah J. Kierans, Alfonso Blanco, Eiva Bernotiene, Orina Belton, Martin Schneider, Eoin P. Cummins, Cormac T. Taylor
Summary: CO2, a major product of respiration, is poorly understood in terms of its biology. Elevated CO2 levels can suppress immune cell activity and migration, and affect macrophage activation through changes in intracellular pH. Patients with chronic lung diseases and elevated systemic CO2 display impaired wound healing and reduced intestinal macrophage infiltration.
JOURNAL OF IMMUNOLOGY
(2022)
