Article
Fisheries
Jiongying Yu, Meng Li, Weimin Wang
Summary: The study revealed that male hybrid loaches were sterile, while the female hybrids showed some fertility but with relatively high deformity rates. Karyotype analysis indicated that fertile gametes produced by female hybrid loaches contained 24 chromosomes.
Article
Fisheries
Wei Luo, Ning Zhang, Dongjie Wang, Zhou Xu, Tianzhu Wang, Xiaoyang Zhang, Tong Gao, Min Liao, Yuejin Long, Zongjun Du
Summary: The hybrid offspring generated from full diallel crosses between different populations of Misgurnus anguillicaudatus and Paramisgurnus dabryanus showed that the P_M x P_Z hybrid had the highest body weight, with high general combining abilities and specific combining ability, demonstrating over 10% heterosis in specific combinations. This study is the first to use microsatellite-based parentage assignment in hybrid fish, providing a foundation for loach crossbreeding.
AQUACULTURE INTERNATIONAL
(2021)
Article
Fisheries
Shuquan Mao, Jiaren Yan, Peng Xu, Yuanyuan Zhang, Liping Song, Bin Hu, Jun Wu, Bingli Wang
Summary: This research compares and analyzes the body index, nutrient composition, and digestive enzyme activity of three types of loaches: Pond loach, Taiwan loach, and Large-scale loach. The study finds that Pond loach has the highest nutritional value and breeding prospects among the three.
ISRAELI JOURNAL OF AQUACULTURE-BAMIDGEH
(2022)
Article
Fisheries
Eun-Mi Kim, Chun Mae Dong, Mi Nan Lee, Jae Koo Noh, Eun Soo Noh, Bo Hye Nam, Young-Ok Kim, Hyo Sun Jung
Summary: We developed a multiplex PCR method for the simultaneous detection of the three most consumed commercial loaches in East Asia: Misgurnus mizolepis, Misgurnus anguillicaudatus, and Paramisgurnus dabryanus. The method involved a phylogenetic analysis of genetic divergence and the design of species-specific PCR primers based on single nucleotide polymorphisms. The results showed successful genetic identification of the three loach species, with different sized PCR products generated by the primers. The multiplex PCR markers developed in this study will be useful for the detection and identification of these loach species.
AQUACULTURE REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Guiyun Huang, Jianmeng Cao, Chen Chen, Miao Wang, Zhigang Liu, Fengying Gao, Mengmeng Yi, Gang Chen, Maixin Lu
Summary: In this study, a genome survey of the dojo loach was conducted using next-generation sequencing technology. The genome size, heterozygosity ratio, repeat sequence content, and GC content were estimated. A large number of microsatellite motifs were identified and tested for polymorphism. In addition, the complete mitochondrial genome was sequenced. The findings of this study will be valuable for further research on population genetics, natural resource conservation, and molecular marker-assisted selective breeding.
MOLECULAR BIOLOGY REPORTS
(2022)
Article
Fisheries
Xiaoli Ke, Jie Liu, Fengying Gao, Jianmeng Cao, Zhigang Liu, Maixin Lu
Summary: Due to environmental pollution and overfishing, the genetic resources of wild loach have been degraded. The study investigated the genetic diversity of different geographical populations of loach in the Pearl River Basin and found that there is abundant genetic diversity, which can be used for breeding. The results provide a theoretical basis for the protection and utilization of loach natural resources in the Pearl River Basin of China.
AQUACULTURE REPORTS
(2022)
Article
Food Science & Technology
Jiahleen B. Roungchun, Amanda M. Tabb, Rosalee S. Hellberg
Summary: Accurate species identification of tuna products is crucial for combating fraud, improving stock regulation, and reducing health risks. This study utilized a DNA mini-barcoding system to successfully identify tuna species in raw and processed samples, with potential for further optimization in future research for canned tuna samples.
Article
Genetics & Heredity
Hu Li, Kai Yu, Rui Zhao, Gang Wu, Chuan-Feng Xiong
Summary: The complete mitochondrial genome of the leafhopper Atkinsoniella nigrita was determined. It consists of typical genes and has a base composition of A = 41.7%, T = 38.2%, C = 10.7%, and G = 9.4%. Compared with other Atkinsoniella species, it shares similarities in certain genes and exhibits a phylogenetic relationship within the genus Atkinsoniella.
MITOCHONDRIAL DNA PART B-RESOURCES
(2023)
Article
Biotechnology & Applied Microbiology
Kelsey J. Andrews, Rachelle Bester, Aruna Manrakhan, Hans J. Maree
Summary: This study successfully identified the five South African fruit fly species through sequence analysis of mitochondrial intergenic regions, with intergenic region I proving to be the most informative region for species differentiation.
Article
Environmental Sciences
Stephane Zingue, Julia Cisilotto, Robinson Charly Mbe Fogang, Edwige Nana Tchoupang, Derek Tantoh Ndinteh, Nestor-Fernand Tchuenguem Fohouo, Dieudonne Njamen, Tania Beatriz Creczynski-Pasa
Summary: Ethanol-extracted Cameroonian propolis demonstrated anti-mammary tumor effects by inducing apoptosis through the mitochondrial pathway, inhibiting MMP-2 activity, and down-regulating certain key proteins. It also prevented tumor migration and invasion, highlighting its potential as a natural treatment for breast cancer.
ENVIRONMENTAL TOXICOLOGY
(2021)
Article
Entomology
Hui Zhang, Qian Liu, Congcong Lu, Jun Deng, Xiaolei Huang
Summary: Lachninae represents an ancient evolutionary lineage of aphids with unique characteristics in their mitochondrial genomes. The newly reported mitogenome of Stomaphis sinisalicis shows significant rearrangements and a tandem repeat region, suggesting ancient features. This study provides valuable insights into mitogenome evolution and serves as a resource for comparative studies across different insect lineages.
Article
Entomology
Dora Henriques, Ana R. Lopes, Nor Chejanovsky, Anne Dalmon, Mariano Higes, Clara Jabal-Uriel, Yves Le Conte, Maritza Reyes-Carreno, Victoria Soroker, Raquel Martin-Hernandez, M. Alice Pinto
Summary: Sequence data from the tRNA(leu)-cox2 intergenic mitochondrial region and SNP data from 91 nuclear DNA loci were collected from 156 colonies in six apiaries across four countries. Analysis focused on genetic patterns, cytonuclear diversity, and admixture levels.
JOURNAL OF APICULTURAL RESEARCH
(2022)
Article
Microbiology
Juliana Moura-Mendes, Cinthia C. Cazal-Martinez, Cinthia Rojas, Francisco Ferreira, Pastor Perez-Estigarribia, Nathalia Dias, Patricio Godoy, Jessica Costa, Cledir Santos, Andrea Arrua
Summary: This study identified and classified the fungi in two maize varieties (Zea mays var. amylacea and Zea mays var. indurata) sold in the market of Paraguay using various methods. It was found that some fungal strains were capable of producing aflatoxins. The results showed that maize marketed in the metropolitan region of Asuncion had high fungal contamination and mycotoxin contamination, posing a potential risk to food safety and public health, and control measures should be implemented.
Article
Food Science & Technology
Dhananjay Kumar, Rajiv Ranjan Kumar, Preeti Rana, S. K. Mendiratta, R. K. Agarwal, Praveen Singh, Sarita Kumari, Jyoti Jawla
Summary: The study developed species-specific RPA-LF assays for identifying the origin of food animals, optimized using Twist Amp basic kit components. The assays detected cattle, buffalo, and pig DNA exclusively, showing high diagnostic sensitivity and correlation with PCR assays. This method can detect target species in meat samples at concentrations as low as 1%.
JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE
(2021)
Article
Zoology
Sergey G. Ermilov, Josef Stary
Summary: This article describes three new species of Pergalumna (Pergalumna) found in highland forests in Central and Southern Malawi. An identification key for known species and subspecies of this genus in the Afrotropical region is also provided.
