期刊
MITOCHONDRIAL DNA PART A
卷 27, 期 4, 页码 2326-2329出版社
TAYLOR & FRANCIS LTD
DOI: 10.3109/19401736.2015.1022764
关键词
COXII-tRNA(Lys) region; mitochondrial DNA; Misgurnus anguillicaudatus; Paramisgurnus dabryanus; species identification
Paramisgurnus dabryanus and Misgurnus anguillicaudatus (family Cobitidae) are loaches with high morphological similarity. In this study, we designed primers to distinguish between Paramisgurnus dabryanus and Misgurnus anguillicaudatus based on the length differences in the mitochondrial COXII to tRNA(Lys) gene region. Samples of P. dabryanus and M. anguillicaudatus from different geographical locations were collected and amplified to verify primer specificity. The results of electrophoresis revealed the successful amplification of all P. dabryanus and M. anguillicaudatus DNA samples, which had distinct, specific-specific sizes (214 bp for P. dabryanus and 285 bp for M. anguillicaudatus). In conclusion, the new primers provide fast, reliable, and accurate identification between P. dabryanus and M. anguillicaudatus.
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