Article
Fisheries
Corinne Audemard, Kimberly S. Reece, Robert J. Latour, Lydia M. Bienlien, Ryan B. Carnegie
Summary: This study investigated the factors influencing the wide range of concentrations of human-pathogenic Vibrio bacteria in oysters. The results suggest that oyster genetics and parasitism may affect the presence of Vibrio bacteria. The study highlights the importance of further investigating the influence of oyster genetic background and the multiple interacting factors driving the dynamics of human-pathogenic Vibrio bacteria in oysters.
Article
Food Science & Technology
Arnau Perez Roig, Hector Carmona-Salido, Eva Sanjuan, Belen Fouz, Carmen Amaro
Summary: This study developed and tested a new method for detecting V. vulnificus, a pathogen linked to aquaculture that poses risks to human and animal health. The method combines a pre-enrichment step with multiplex PCR, demonstrating high specificity and a low detection limit. The method was successfully validated in seafood and shown to be useful in pathogen isolation from animals and their ecosystem.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2022)
Article
Microbiology
Annamaria Castello, Vincenzina Alio, Sonia Sciortino, Giuseppa Oliveri, Cinzia Cardamone, Gaspare Butera, Antonella Costa
Summary: The aim of this study was to evaluate the spread of potentially pathogenic Vibrio spp. in fishery products collected in Sicily and to assess their antibiotic resistance. Bacteriological and molecular methods were applied to 603 seafood samples to detect V. parahaemolyticus, V. cholerae, V. vulnificus, and Vibrio alginolyticus in order to assess their pathogenicity and antimicrobial resistance. The results showed a significant contamination of Vibrio spp. in the seafood samples, with some strains exhibiting resistance to commonly used antibiotics.
Article
Environmental Sciences
Md. Ashrafudoulla, Kyung Won Na, Md. Iqbal Hossain, Md. Furkanur Rahaman Mizan, Shamsun Nahar, Sazzad Hossen Toushik, Pantu Kumar Roy, Si Hong Park, Sang -Do Ha
Summary: Infections of gastroenteritis in humans are mainly caused by consumption of shellfish contaminated with Vibrio parahaemolyticus, leading to health and economic losses. Investigation on the virulence factor production, antibiotic resistance profile, and biofilm-forming capacity of Vibrio parahaemolyticus isolates showed high resistance to antibiotics and virulence factors in isolates from shellfish in Korea. Monitoring of these factors may help in decreasing V. parahaemolyticus infections and ensuring seafood safety.
MARINE POLLUTION BULLETIN
(2021)
Article
Food Science & Technology
Tong Wang, Shuo Wan, Jian-Lian Huang, Zhi-Hai Sui, Cui-Juan Gao, Yun-Guo Liu
Summary: This study investigated and analyzed the pollution status of pathogenic Vibrio in marine products in China, and found that pathogenic Vibrio contamination existed in different regions, different types of aquatic products, and different sampling locations. The detection rate of the pathogen was statistically analyzed. This provides a theoretical basis for food safety supervision departments to develop targeted prevention and control measures.
JOURNAL OF FOOD QUALITY
(2022)
Article
Biotechnology & Applied Microbiology
Lydia M. Bienlien, Corinne Audemard, Kimberly S. Reece, Ryan B. Carnegie
Summary: The study found that the prevalent parasite P. marinus had little influence on human-pathogenic Vibrio species levels in eastern oysters, but the less common parasite, H. nelsoni, may impact V. vulnificus levels, highlighting the potential nuances of within-oyster dynamics of Vibrio species.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Karanth Padyana Anupama, Ashwath Nayak, Iddya Karunasagar, Indrani Karunasagar, Biswajit Maiti
Summary: The study successfully developed a LAMP assay for detecting pathogenic Vibrio parahaemolyticus, which exhibited good specificity and high sensitivity, enabling direct detection of the pathogen in seafood samples.
MOLECULAR BIOLOGY REPORTS
(2021)
Article
Biotechnology & Applied Microbiology
Hudaa Neetoo, Keshnee Reega, Zishaan Sheik Manoga, Nndeem Nazurally, Vishwakalyan Bhoyroo, Mushal Allam, Yasmina Jaufeerally-Fakim, Anisah Wahed Ghoorah, Wasseem Jaumdally, Aicha Malleck Hossen, Farheen Mayghun, Arshad Ismail, Muzzammil Hosenally
Summary: This study aimed to determine the prevalence, population densities, species diversity, and molecular characteristics of pathogenic Vibrio in various seafood commodities and the health risks associated with consumption of these contaminated commodities. The results showed that the population of pathogenic Vibrio in seafood is influenced by season, with higher levels in the summer. Additionally, certain strains of Vibrio found in the seafood samples carried virulence and antibiotic resistance genes, raising concerns about public health. Overall, this study highlights the widespread presence and diversity of Vibrio in seafood and the potential risks they pose.
JOURNAL OF FOOD PROTECTION
(2022)
Article
Food Science & Technology
Regina Klapper, Ute Schroder
Summary: Seafood products are vulnerable to food fraud, highlighting the importance of reliable authentication methods. This study developed a multiplex TaqMan real-time PCR assay for rapid identification of commercially important scallop species.
Article
Food Science & Technology
Christian Brenn, Ute Schroeder, Reinhold Hanel, Pedro Martinez Arbizu
Summary: The study presents a TaqManprobe based multiplex real-time PCR screening assay for identification of four commercially relevant crustacean species, showing high specificity and potential for rapid species identification.
Article
Food Science & Technology
Pengzhen Li, Xiaoxuan Feng, Baiyan Chen, Xiaoying Wang, Zuyue Liang, Li Wang
Summary: A multiplex PCR system was developed for simultaneous detection of eight foodborne pathogenic bacteria. The system exhibited high sensitivity and specificity, and showed good stability and sensitivity in artificially contaminated samples.
Article
Biochemical Research Methods
Hongxia Guan, Panpan Xue, Haijian Zhou, Dan Sha, Duochun Wang, He Gao, Jie Li, Baowei Diao, Hongqun Zhao, Biao Kan, Jingyun Zhang
Summary: A multiplex PCR assay was developed to detect five pathogenic Vibrio species and Plesiomonas shigelloides, showing high specificity and sensitivity in various samples. It outperformed conventional cultivation methods in detection and can be applied for screening enrichment cultures and suspected colonies from different sources.
MOLECULAR AND CELLULAR PROBES
(2021)
Article
Food Science & Technology
Ning Yuan, Haoyu Yang, Yunzhe Zhang, Hancong Xu, Xin Lu, Hui Xu, Wei Zhang
Summary: A rapid detection method for V. parahaemolyticus based on RF-SRCA technology has been established in this study. Real-time fluorescence curve can effectively determine the results within a short time, and the method exhibits excellent specificity and sensitivity. RF-SRCA has higher sensitivity and shorter detection time compared to traditional methods, and can eliminate the risk of cross-contamination.
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Microbiology
Yong Yan, Li Zhan, Guoying Zhu, Junyan Zhang, Ping Li, Lixia Chen, Peiyan He, Jianyong Luo, Zhongwen Chen
Summary: A novel multiplex assay for rapid detection of Vibrio cholerae serogroup and cholera toxin toxigenicity was developed. The assay showed good diagnostic sensitivities and is suitable for rapid identification and screening of large specimens.
Article
Biotechnology & Applied Microbiology
Sunyoung Park, Heeyoung Lee, Sejeong Kim, Yukyung Choi, Hyemin Oh, Yujin Kim, Yewon Lee, Yeongeun Seo, Joohyun Kang, Eunyoung Park, Yohan Yoon, Jimyeong Ha
Summary: The study identified the pathogenic tdh gene in Vibrio parahaemolyticus isolates, with variations in amino acids influencing pathogenicity in terms of adherence and cytotoxicity. The isolates with these variations may cause more serious foodborne illness compared with other strains. Amino acid variations at regions related to the toxic gene were found to make a difference in pathogenicity.
JOURNAL OF FOOD PROTECTION
(2021)
Review
Biochemical Research Methods
Y. Seesao, M. Gay, S. Merlin, E. Viscogliosi, C. M. Aliouat-Denis, C. Audebert
JOURNAL OF MICROBIOLOGICAL METHODS
(2017)
Article
Fisheries
Michela Paoletti, Simonetta Mattiucci, Alessandra Colantoni, Arne Levsen, Melanie Gay, Giuseppe Nascetti
FISHERIES RESEARCH
(2018)
Article
Fisheries
Arne Levsen, Paolo Cipriani, Simonetta Mattiucci, Melanie Gay, Lee C. Hastie, Ken MacKenzie, Graham J. Pierce, Cecilie S. Svanevik, Danjal P. Hojgaard, Giuseppe Nascetti, Angel F. Gonzalez, Santiago Pascual
FISHERIES RESEARCH
(2018)
Article
Fisheries
Simonetta Mattiucci, Lucilla Giulietti, Michela Paoletti, Paolo Cipriani, Melanie Gay, Arne Levsen, Regina Klapper, Horst Karl, Miguel Bao, Graham J. Pierce, Giuseppe Nascetti
FISHERIES RESEARCH
(2018)
Article
Fisheries
M. Gay, M. Bao, K. MacKenzie, S. Pascual, K. Buchmann, O. Bourgau, C. Couvreur, S. Mattiucci, M. Paoletti, L. C. Hastie, A. Levsen, G. J. Pierce
FISHERIES RESEARCH
(2018)
Article
Microbiology
Gabriela Certad, Jerome Follet, Nausicaa Gantois, Ourida Hammouma-Ghelboun, Karine Guyot, Sadia Benamrouz-Vanneste, Emilie Frealle, Yuwalee Seesao, Baptiste Delaire, Colette Creusy, Gael Even, Veronique Verrez-Bagnis, Una Ryan, Melanie Gay, Cecile Aliouat-Denis, Eric Viscogliosi
FRONTIERS IN MICROBIOLOGY
(2019)
Article
Microbiology
Nausicaa Gantois, Angelique Lamot, Yuwalee Seesao, Colette Creusy, Luen-Luen Li, Sebastien Monchy, Sadia Benamrouz-Vanneste, Jacky Karpouzopoulos, Jean-Luc Bourgain, Celia Rault, Fabien Demaret, Martha Baydoun, Magali Chabe, Emilie Frealle, Cecile-Marie Aliouat-Denis, Melanie Gay, Gabriela Certad, Eric Viscogliosi
Article
Medicine, General & Internal
J. Dupouy-Camet, M. Gay, R. Houin
BULLETIN DE L ACADEMIE NATIONALE DE MEDECINE
(2020)
Article
Fisheries
Maureen Duflot, Graziella Midelet, Odile Bourgau, Kurt Buchmann, Melanie Gay
Summary: The study optimized the pepsin digestion protocol and assessed the viability duration of Cryptocotyle metacercariae in fish post-mortem. It found that the orbital digestion method was the least destructive and allowed better quantification of Cryptocotyle infection. Morphological identification appeared reliable up to 8 days post-mortem for Cryptocotyle infection.
JOURNAL OF FISH DISEASES
(2021)
Review
Biochemical Research Methods
Maureen Duflot, Thomas Setbon, Graziella Midelet, Thomas Brauge, Melanie Gay
Summary: The superfamily Opisthorchioidea includes various parasitic families, with some species having a significant impact on global public health, leading to the need for research and tool development. While morphological descriptions remain important, molecular approaches have become common for parasite identification.
JOURNAL OF MICROBIOLOGICAL METHODS
(2021)
Article
Parasitology
Maureen Duflot, Melanie Gay, Graziella Midelet, Per Walter Kania, Kurt Buchmann
Summary: This study reported the morphological and molecular identifications of encysted black spot-inducing parasites in marine fish caught from the English Channel and Danish sea waters. It is the first description of C. lingua metacercariae in the English Channel ecosystems.
PARASITOLOGY RESEARCH
(2021)
Article
Fisheries
Pierre Cresson, Odile Bourgau, Remy Cordier, Clemence Couvreur, Manuel Rouquette, Melanie Gay
Summary: Parasites play an important role in marine systems, but their impact is often overlooked. This study focused on understanding the factors driving parasite infection in fish, specifically Anisakis levels in Atlantic horse mackerel. The results showed that ontogenic dietary shifts, depth, and lipid content were all important drivers of parasite infection in these fish.
CANADIAN JOURNAL OF FISHERIES AND AQUATIC SCIENCES
(2023)
Article
Parasitology
Maureen Duflot, Pierre Cresson, Maeva Julien, Lea Chartier, Odile Bourgau, Marialetizia Palomba, Simonetta Mattiucci, Graziella Midelet, Melanie Gay
Summary: Fish can develop black spots due to infection by metacercarial trematodes, including Cryptocotyle spp. However, the impact of these parasites on human health is still unknown. This study conducted an epidemiological survey on seven commercial fish species in the English Channel and North Sea, and found a prevalence of 20.5% for encysted metacercariae, including two species of Cryptocotyle. The results contribute to a better understanding of the ecology and distribution of these parasites.
Article
Fisheries
Claudia Gerard, Maxime R. Herve, Heloise Hamel, Melanie Gay, Michel Barbier, Thomas Barreau
Summary: The parasitofauna of the near-threatened coastal shark Mustelus asterias in Europe is largely unknown. In this study, metazoan parasites of immature sharks from the English Channel were examined, revealing a diverse range of parasites. The parasite community differed between males and females, possibly indicating early spatial sex-segregation. Parasites provide valuable information on diet ecology and stock discrimination of sharks.
AQUATIC LIVING RESOURCES
(2022)
Review
Food Science & Technology
Maryse Bonnin-Jusserand, Stephanie Copin, Cedric Le Bris, Thomas Brauge, Melanie Gay, Anne Brisabois, Thierry Grard, Graziella Midelet-Bourdin
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2019)
Article
Food Science & Technology
Guangfei Wei, Yichuan Liang, Guozhuang Zhang, Zhaoyu Zhang, Yongqing Zhang, Shilin Chen, Linlin Dong
Summary: This study investigated the presence of fungi on the surface of Polygonum multiflorum Thunb and found that it is easily contaminated by toxigenic fungi and mycotoxins, posing a health risk for consumers. The results also showed that sampling location and processing can influence the assembly and network complexity of the surface microbiome of Polygonum multiflorum Thunb.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Article
Food Science & Technology
Gabriele Busetta, Giuliana Garofalo, Salvatore Claps, Maria Teresa Sardina, Elena Franciosi, Antonio Alfonzo, Nicola Francesca, Giancarlo Moschetti, Luca Settanni, Raimondo Gaglio
Summary: This study evaluates the microbial diversity of wooden shelves used for the ripening of PDO Pecorino di Filiano and PGI Canestrato di Moliterno cheeses. The results show a strict correlation between the microbiota of wooden shelves and cheese rinds, indicating the positive role of wooden shelves in the cheese ripening process.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Article
Food Science & Technology
Massimo Todaro, Giuliana Garofalo, Gabriele Busetta, Riccardo Gannuscio, Ambra Rita Di Rosa, Maria Luisa Scatassa, Cinzia Cardamone, Isabella Mancuso, Elena Franciosi, Fatima Rando, Monica Agnolucci, Vincenzo Chiofalo, Raimondo Gaglio, Luca Settanni
Summary: This study aimed to reduce the production time of PDO Pecorino Siciliano cheese by comparing two cooking methods and assessing their impact on microbiological composition and lactic acid bacteria populations. The results showed that the different cooking methods did not affect the physicochemical characteristics of the cheese, and sensory evaluation confirmed that the modified production protocol did not significantly alter the product characteristics and overall acceptance. Therefore, cooking under hot water proved to be an effective way to reduce the transformation duration while maintaining the typicality of PDO Pecorino Siciliano cheese.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Article
Food Science & Technology
Yingying Sun, Yana Liu, Weiwei Zhou, Lele Shao, Han Wang, Yijie Zhao, Bo Zou, Xingmin Li, Ruitong Dai
Summary: The study evaluates the effect of Ohmic heating (OH) at various voltage gradients and water bath on microbial inactivation, physicochemical and sensory properties, and microbial flora of pasteurized milk. Results show that OH with higher voltage effectively inactivates microorganisms, slows down lipid oxidation, and maintains sensory quality and amino acids content of milk. The microbial community in milk treated with OH remains relatively stable during storage.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Article
Food Science & Technology
Minjin Oh, Ana Cevallos-Urena, Byoung Sik Kim
Summary: In this study, two bacteriophages, PECP14 and PECP20, infecting Escherichia coli O157:H7 were isolated and found to be stable under different environmental conditions. These phages exhibited specificity in targeting different host receptors and demonstrated the ability to infect other foodborne pathogens. The efficacy of phage treatment was demonstrated through rapid adsorption and bacterial lysis. Furthermore, purified endolysins derived from these phages showed remarkable bacteriolytic activity not only against E. coli O157:H7 but also against other pathogens. These findings suggest that these phages and their endolysins can be used for biological control of E. coli O157:H7 in various stages of agricultural product production.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Review
Food Science & Technology
Filippo Marzoli, Michela Bertola, Juliane Pinarelli Fazion, Giulia Cento, Pietro Antonelli, Beatrice Dolzan, Lisa Barco, Simone Belluco
Summary: Insects are a sustainable and protein-rich food source, and Salmonella is a common pathogen in insects. This systematic review aims to collect and evaluate existing studies on the occurrence of Salmonella in crickets and mealworms. Although the number of studies is limited and there are some limitations, this review highlights the importance of obtaining reliable data on the presence of Salmonella in insects, considering the growing market and increased farming scale.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Review
Food Science & Technology
Lucas Chikhi, Melanie Mancier, Hubert Brugere, Bertrand Lombard, Lyazrhi Faouzi, Laurent Guillier, Nathalie Gnanou Besse
Summary: Listeriosis is a severe foodborne disease with a high mortality rate, mainly caused by L. monocytogenes. The EU regulation sets qualitative or quantitative food safety criteria for L. monocytogenes and recommends the use of EN ISO 11290-1 as the reference detection method. Our study compares alternative certified methods for detecting L. monocytogenes, highlighting the importance of diversifying available detection methods.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Article
Food Science & Technology
Christina A. M. DeWitt, Kevin A. Nelson, Hyung Joo Kim, David H. Kingsley
Summary: High pressure processing (HPP) is a non-thermal method that can effectively inactivate pathogens. Research has shown that applying high pressure to thawed samples in an ultra-low temperature environment enhances the inactivation of norovirus.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Article
Food Science & Technology
Suzane Manzini, Thaina Valente Bertozzo, Isabella Neves Aires, Nassarah Jabur Lot Rodrigues, Amanda Bezerra Bertolini, Marcela Alexandrino, Jackieline Sampaio Steinle, Renata Pimentel Bandeira de Melo, Rinaldo Aparecido Mota, Maria Izabel Merino de Medeiros, Virginia Bodelao Richini-Pereira, Vera Claudia Lorenzetti Magalhaes Curci, Simone Baldini Lucheis
Summary: This study detected Toxoplasma gondii DNA in raw bovine milk samples from expansion tanks in small properties located in different cities of the Midwest region of Sa similar to o Paulo, Brazil using PCR. The results showed that the TgNP1 and TgNP2 genes were more efficient in detecting T. gondii DNA, highlighting the importance of raw bovine milk as a potential source of human infections caused by this parasite, and emphasizing the need for proper pasteurization and further understanding of the epidemiology of this protozoan.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Article
Food Science & Technology
Luca Bolzoni, Mauro Conter, Luca Lamperti, Erika Scaltriti, Marina Morganti, Antonio Poeta, Marco Vecchi, Silvia Paglioli, Alessandra Rampini, Paolo Ramoni, Daniela De Vita, Cristina Bacci, Martina Rega, Laura Andriani, Stefano Pongolini, Silvia Bonardi
Summary: This study assessed the role of equids as carriers of Salmonella and the occurrence of contaminated equid carcasses during the slaughter process. Salmonella was detected in a small percentage of equids' caecal contents, but not in carcass samples. Additionally, a genetic match was found between Salmonella strains from horse caecal contents and human cases, suggesting a link between horse meat consumption and salmonellosis.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Article
Food Science & Technology
Daria Nowinski, Tomasz Czapka, Irena Maliszewska
Summary: The effect of multiple sublethal doses of non-thermal plasma treatments on phenotypical changes and the reduction in phytopathogenicity of three species of fungi was investigated. The plasma damaged the surface of the fungal hyphae, decreased their dry biomass, and affected the activity of pectinase and xylanase. Furthermore, the plasma treatments increased the germination rate of artificially infected cucumber seeds and resulted in plants with higher biomass and longer roots and stems. However, the reduction in phytopathogenicity was not complete after the multiple plasma treatments.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)