Article
Infectious Diseases
I. E. Ramirez-Bayard, F. Mejia, J. R. Medina-Sanchez, H. Cornejo-Reyes, M. Castillo, J. Querol-Audi, A. O. Martinez-Torres
Summary: Antimicrobial resistance bacteria, including Escherichia coli, are widespread and pose a global health and economic burden. Monitoring fecal microbiota is important as it can serve as a potential source of resistant E. coli due to gene transfer between different bacteria. An analysis of 150 E. coli isolates from environmental, animal, and human samples in rural areas of Panama revealed high resistance to tetracycline and the presence of plasmid-associated tetA and tetB genes, suggesting the horizontal transfer of resistance genes within the community.
Article
Immunology
S. Sreejith, Shamna Shajahan, P. R. Prathiush, V. M. Anjana, Jyothis Mathew, S. Aparna, Swapna Susan Abraham, E. K. Radhakrishnan
Summary: Containing antimicrobial resistance is crucial as it affects various aspects of human life. This study developed a PCR-based method for the rapid screening and antibiotic stewardship of poultry samples. Results showed the potential of this method in detecting antibiotic resistance genes in livestock farms.
MICROBIAL PATHOGENESIS
(2022)
Article
Microbiology
Nawarat Somprasong, Carina M. Hall, Jessica R. Webb, Jason W. Sahl, David M. Wagner, Paul Keim, Bart J. Currie, Herbert P. Schweizer
Summary: Burkholderia ubonensis, a nonpathogenic soil bacterium, contains a novel tetracycline-specific efflux pump gene tetA(64), which, in combination with other resistance genes, confers high resistance to tetracycline and doxycycline. The gene was acquired through homologous recombination and provides bacteria with defense against high concentrations of tetracycline under specific conditions.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Agriculture, Dairy & Animal Science
Antonietta Di Francesco, Daniela Salvatore, Sonia Sakhria, Elena Catelli, Caterina Lupini, Mohamed Salah Abbassi, Ghaith Bessoussa, Salma Ben Yahia, Noureddine Ben Chehida
Summary: The widespread use of tetracyclines in clinical practice and livestock has led to an increase in tetracycline resistance among bacterial populations. A study in Tunisia tested DNA from cloacal swabs of 195 broiler chickens for 14 tetracycline resistance genes, revealing a high frequency and diversity of these genes. This highlights the urgent need for a robust national antimicrobial resistance monitoring plan in Tunisia's poultry sector.
Article
Infectious Diseases
Ruichao Li, Yongjia Jiang, Kai Peng, Yanhong Wang, Mianzhi Wang, Yuan Liu, Zhiqiang Wang
Summary: This study confirms the high prevalence of tet(X) in R. anatipestifer. Further investigations are needed to assess the transfer risk of tet(X) from R. anatipestifer to other clinical pathogens.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2022)
Article
Agriculture, Dairy & Animal Science
Antonietta Di Francesco, Daniela Salvatore, Fabrizio Bertelloni, Valentina Virginia Ebani
Summary: In this study, a culture-independent approach was used to investigate the spread of tetracycline resistance genes in wild birds, with 114 out of 254 birds tested showing positivity for one or more genes. Wild animals, including birds, can act as reservoirs, dispersers, or bioindicators of antimicrobial resistance in the environment. The implications of antimicrobial resistance in wildlife and the need for careful consideration of resistance control strategies are highlighted.
Article
Chemistry, Physical
Xiaojing Zheng, Fangning Feng, Honghui Yang
Summary: Improving the rate of photo-fermentative hydrogen production has been a major challenge in the field of biological hydrogen production using photosynthetic bacteria (PSB) in recent years. In a previous study, it was observed that the expression of tetA gene in R. sphaeroides HY01 under tetracycline (Tc) led to significant enhancement of hydrogen production. Further investigation revealed that Tc-resistant strains consistently showed stable promotion of hydrogen production rate (Rm), with approximately 30% promotion with Tc addition. This study explores the Tc dose-dependent hydrogen promotion of resistant strains and investigates the gene expression differences induced by TetA expression and Tc addition in Rodobacter sphaeroides.
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
(2023)
Article
Biotechnology & Applied Microbiology
Ahamed Basha Kusunur, Shanmukha Sai Mogilipuri, Dhamodher Moturu, Manikantha Benala, Murugadas Vaiyapuri, Satyen Kumar Panda, Joshy Chalil George, Madhusudana Rao Badireddy
Summary: This study investigated the tetracycline resistance potential of heterotrophic bacteria isolated from freshwater fin-fish culture ponds in Andhra Pradesh, India. The results showed that Lactococcus garvieae was the predominant bacteria with high tetracycline resistance, followed by Enterobacter spp., Lactococcus lactis, and Staphylococcus arlettae. The tet(A) gene was the major determinant of tetracycline resistance. The tet(HR) bacteria showed cross-resistance to furazolidone, co-trimoxazole, and enrofloxacin.
JOURNAL OF APPLIED MICROBIOLOGY
(2023)
Article
Chemistry, Analytical
Qianji Li, Xiwen Wu, Xiaohuan Zhang, Wenen Su, Yan Tan, Pengfei Fan, Jinquan Liu, Shengyuan Yang
Summary: Eco-friendly biomass carbon dots (CDs) with blue fluorescence emission were synthesized using a microwave method, and their fluorescence could be selectively quenched by oxytetracycline (OTC) based on the inner filter effect (IFE) between OTC and CDs. A simple and time-saving fluorescence sensing system for detecting OTC was established, showing good linearity, high sensitivity, and a low detection limit. This method has the advantages of low cost, time-saving, and green synthesis, and it has potential applications in food safety.
ANALYTICAL METHODS
(2023)
Article
Genetics & Heredity
Antony T. Vincent, Romain P. Bergeron, Laurie C. Piche, David Prado, Linda Saucier
Summary: This study analyzes the genomic level of the tetracycline-resistant strain B. thermosphacta BT469 to understand the molecular determinants responsible for this resistance. Three plasmids have been identified in the strain, with pBT469-2 being unique to the BT469 strain. Similar plasmid sequences have also been found in other bacterial genera, suggesting a diverse family of plasmids present in multiple bacterial genera.
Article
Infectious Diseases
Gazi Sofiul Alam, Mohammad Mahmudul Hassan, Md. Ahaduzzaman, Chandan Nath, Pronesh Dutta, Hamida Khanom, Shahneaz Ali Khan, Md Ridoan Pasha, Ariful Islam, Ricardo Soares Magalhaes, Rowland Cobbold
Summary: This study investigated the AMR patterns and tetracycline-resistant gene profiles of E. coli in broiler meat and livers from live bird markets and supermarkets in Chattogram, Bangladesh. The results showed high prevalence of resistance against multiple antibiotics and the presence of multi-drug resistant E. coli strains. It also revealed the widespread presence of tetracycline-resistant genes in E. coli isolates. This highlights the need for proper management of antimicrobial use in poultry farming and emphasizes the public health risks associated with AMR in broiler-associated pathogens.
Review
Infectious Diseases
Ishani Wickramage, Patrizia Spigaglia, Xingmin Sun
Summary: Clostridioides difficile (CD) is a major concern as one of the top five antibiotic resistance threats in the USA, with a global increase in multidrug resistance and emergence of more virulent strains. Understanding the evolving antibiotic resistance mechanisms of CD is crucial for guiding antibiotic stewardship policies and developing new therapeutic targets. Epidemiological surveillance and strain-specific characteristics are key in combating the spread of resistance genes among different CD strains.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2021)
Article
Multidisciplinary Sciences
Rossella Filardi, Giorgio Gargari, Diego Mora, Stefania Arioli
Summary: Akkermansia muciniphila, a commensal bacterium commonly found in healthy gut microbiota, is considered a potential beneficial bacterium to improve metabolic and inflammatory disorders. Recently, it has been declared safe as a novel food, opening the door to its commercialization. However, there is still limited information about the antimicrobial susceptibility of this species, highlighting the need for further research to assess its safety.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Applied
Zhengzheng Zhao, Zhifang Wu, Xueling Lin, Fangjie Han, Zhishan Liang, Likun Huang, Mengjiao Dai, Dongxue Han, Lipeng Han, Li Niu
Summary: A simple, low-cost label-free photoelectrochemical biosensing platform based on red blood cell shaped BiVO4 modified g-C3N4 was designed and demonstrated for tetracycline detection. The prepared g-C3N4/BiVO4 heterojunction showed high surface area, excellent stability, and favorable PEC activity. It also served as a nanostructure support for aptamers to construct a visible-light-driven PEC aptasensor. The proposed PEC aptasensor exhibited a favorable linear response to tetracycline and met the detection requirements of food standards. Practical food sample analysis confirmed the accuracy and feasibility of the g-C3N4/BiVO4 heterostructure based PEC platform. This label-free and cost-effective PEC strategy holds great promise for tetracycline determination in food quality control and supervision.
Article
Chemistry, Applied
Yunlian Zhang, Md Mehedi Hassan, Yawen Rong, Rui Liu, Huanhuan Li, Qin Ouyang, Quansheng Chen
Summary: An upconversion nanosensor for detecting tetracycline was developed using magnetic separation and electrostatic adsorption, with a linear detection range of 0.5-1000 ng mL(-1) and a detection limit of 0.17 ng mL(-1). This method showed great potential for tetracycline detection in food samples, providing simple operation, high sensitivity, excellent repeatability, and selectivity.
Article
Chemistry, Multidisciplinary
Juan Francisco Flores-Aguilar, Gabriela Islas, Jose Antonio Rodriguez, Maria Elena Paez-Hernandez, Carlos Andres Galan-Vidal, Israel Samuel Ibarra
Summary: A lead ion-imprinted polymer based on methacrylic acid and 4-vinylpyridine has been synthesized and used for the determination of lead in infant formula samples. The proposed method showed low detection limit and good repeatability.
JOURNAL OF THE MEXICAN CHEMICAL SOCIETY
(2022)
Article
Biochemistry & Molecular Biology
Carolina Nebot, Alejandra Cardelle-Cobas, Ignacio Garcia-Presedo, Ewelina Patyra, Alberto Cepeda, Carlos M. Franco
Summary: Veterinary drugs are commonly used in food-producing animals to prevent and treat diseases. Analyzing pharmaceutical residues in animal manure can help evaluate legal and illegal practices in food production without harming animals. A method for simultaneously analyzing 29 active substances in manure samples was described in this article, with antibiotics and antiparasitic agents being the main focus. The method was validated and showed potential for controlling the administration of pharmaceuticals in food-producing animals.
Article
Chemistry, Organic
Jorge Lopez-Tellez, Israel S. S. Ibarra, Julian Cruz-Borbolla, Marisol Vega, Jose A. A. Rodriguez
Summary: This study proposes to use recycled EPS to modify the surface of PUFs for the retention of 12 PAHs in air samples. The modified material is suitable for the retention-elution of PAHs from air samples and can be used in liquid chromatography for quantitative analysis. Eighteen urban air samples were collected and analyzed weekly, and a relationship between meteorological parameters and PAH concentration was observed using PCA.
POLYCYCLIC AROMATIC COMPOUNDS
(2023)
Article
Biochemistry & Molecular Biology
Jaime Ortiz-Viedma, Jose M. Bastias-Montes, Cielo Char, Camila Vega, Alejandra Quintriqueo, Manuela Gallon-Bedoya, Marcos Flores, Jose M. Aguilera, Jose M. Miranda, Jorge Barros-Velazquez
Summary: A biorefinery process was developed for utilizing freeze-dried pomace of calafate berries. The process involved extraction of lipophilic components using supercritical CO2 and subsequent extraction of the residue using a pressurized mixture of ethanol/water. This eco-friendly process valorizes calafate pomace as a source of ingredients for healthy foods, nutraceuticals, and nutritional supplements.
Article
Food Science & Technology
Roberto Bermudez, Esmeralda Rangel-Vargas, Jose M. Lorenzo, Jose A. Rodriguez, Paulo E. S. Munekata, Alfredo Teixeira, Mirian Pateiro, Leticia Romero, Eva M. Santos
Summary: The effects of adding Hibiscus sabdariffa by-product (2-5%) and Pleurotus djamor powder (5-7.5%) as meat replacers to beef patties were analyzed. The addition of these non-meat ingredients decreased moisture and increased fiber content, without affecting the protein level. While the roselle by-product had limited antioxidant effect, Pleurotus djamor favored oxidation processes. The samples with these ingredients had lower microbial counts, but were significantly affected in terms of texture and sensorial parameters.
Review
Nutrition & Dietetics
Shahrzad Ezzatpour, Alicia del Carmen Mondragon Portocarrero, Alejandra Cardelle-Cobas, Alexandre Lamas, Aroa Lopez-Santamarina, Jose Manuel Miranda, Hector C. Aguilar
Summary: The human gastrointestinal tract contains diverse microorganisms that interact with the host and play important roles in regulating metabolic processes. While the gut bacteriome has been extensively studied, there is increasing interest in understanding the influence of other microbial groups, such as the gut virome and phages, on the host immune system and metabolic functions. Investigating the role of virome-rectification-based therapies in various diseases is also important. In future research, stool metagenomic investigations should focus on identifying bacteria and phages, as well as studying their correlation networks, to gain a better understanding of gut microbiota activity in metabolic disease progression.
Article
Environmental Sciences
Dulce Brigite Ocampo-Rodriguez, Gabriela A. Vazquez-Rodriguez, Jose Antonio Rodriguez, Maria del Refugio Gonzalez Sandoval, Ulises Iturbe-Acosta, Sylvia Martinez Hernandez, Claudia Coronel-Olivares
Summary: This study compared the removal efficacy of sodium hypochlorite and peracetic acid on three strains of bacteria isolated from wastewater. The results showed that both disinfectants were effective in inactivating S. pasteuri, but peracetic acid showed greater resistance for K. pneumoniae and was more effective for B. subtilis endospores.
Article
Biochemistry & Molecular Biology
Daniela Castro-Enriquez, Jose M. Miranda, Marcos Trigo, Francisco Rodriguez-Felix, Santiago P. Aubourg, Jorge Barros-Velazquez
Summary: This study investigated the use of pitaya extracts in biodegradable packing films to inhibit quality loss in fish muscle during refrigerated storage. The experiments utilized pitaya-gelatin films for hake muscle storage and pitaya-polylactic acid (PLA) films for Atlantic mackerel muscle storage. The results showed that the presence of pitaya extract led to a significant inhibitory effect on lipid damage, microbial activity development, and growth of bacteria in both systems. The preservation effects were attributed to the presence of betalains and phenolic compounds in the pitaya extracts.
Article
Food Science & Technology
Sarah Azinheiro, Pedro Rodriguez-Lopez, Antonio Lozano-Leon, Hugo Guedes, Patricia Regal, Carlos M. Franco, Alberto Cepeda, Pilar Teixeira, Luis D. R. Melo, Daniela Silva, Ana Fernandez, Marcia Faria, Foteini Roumani, Juan Herrera, Marta Prado, Marta Lopez-Cabo, Alejandro Garrido-Maestu
Summary: Listeria monocytogenes is a significant foodborne pathogen that infects vulnerable individuals through contaminated foods. A new qPCR-based method for detecting L. monocytogenes was evaluated in a study involving six laboratories in Spain and Portugal. The results showed high sensitivity and agreement with the reference method, indicating its potential for controlling the spread of L. monocytogenes.
Article
Nutrition & Dietetics
Laura Sinisterra-Loaiza, Patricia Alonso-Lovera, Alejandra Cardelle-Cobas, Jose Manuel Miranda, Beatriz I. Vazquez, Alberto Cepeda
Summary: Various studies have found that specific groups or dietary compounds play a role in the onset and progression of obesity, and gut microbiota serves as a mediator between these compounds and the associated inflammation. This study aimed to evaluate the dietary intake of overweight, obese, and normal-weight individuals and analyze their gut microbiota profile in relation to changes in Body Mass Index (BMI) and diet. The results showed significant differences in fiber and monounsaturated fatty acids (MUFAs) intake among different BMI groups, with better intake adequacy observed in the normal-weight group. The analysis of gut microbiota revealed distinct microbial composition in overweight and obese individuals compared to normal-weight individuals, with specific genera identified as biomarkers for each group.
Article
Polymer Science
Irma Perez-Silva, Gladis D. Canales-Feliciano, Jose A. Rodriguez, Luis H. Mendoza-Huizar, Salvador Perez-Estrada, Israel S. Ibarra, M. Elena Paez-Hernandez
Summary: Cellulose acetate, derived from cellulose, is biodegradable and reusable. Functionalized capsules with Cyanex 923 were able to effectively remove and recover cadmium. Different variables, such as HCl concentration and carrier content, influenced the extraction efficiency of cadmium. The study also tested three isotherm models for modeling the cadmium removal process.
Article
Food Science & Technology
Yuliya Pronina, Talgat Kulazhanov, Zhanar Nabiyeva, Olga Belozertseva, Anastasiya Burlyayeva, Alberto Cepeda, Erik Askarbekov, Gulzhan Urazbekova, Elmira Bazylkhanova
Summary: The proper functioning of the gastrointestinal tract is crucial for strengthening the immune system. Lactic acid microorganisms are necessary for this proper functioning, mainly sourced from dairy products. However, lactose digestibility poses a problem, leading to the interest in alternative sources and carriers of probiotics. Protein marshmallow can serve as such a carrier due to its dietary and natural properties. This study aims to identify the dependence of technological factors on the rheological properties of the product and the growth of lactic acid microorganisms in confectionery products enriched with lyophilised strains.
Article
Food Science & Technology
Deep Das, Abimannan Arulkumar, Sadayan Paramasivam, Aroa Lopez-Santamarina, Alicia del Carmen Mondragon, Jose Manuel Miranda Lopez
Summary: This study evaluated the phytochemical constituents, antioxidant, and antibacterial properties of Kappaphycus alvarezii and Cymodocea serrulata. Both seaweeds contained flavonoids, tannins, phenolic compounds, glycosides, steroids, carbohydrates, and ashes. Alkaloids were only found in K. alvarezii. The chloroform extracts of both seaweeds exhibited antagonistic efficacy against Bacillus subtilis, Vibrio parahaemolyticus, Vibrio alginolyticus, Vibrio harveyi, and Klebsiella pneumoniae. GC-MS analysis revealed the presence of numerous bioactive compounds in both seaweeds, which contributed to their antioxidant and antibacterial activities. Based on the results, K. alvarezii and C. serrulata could be considered as valuable sources of nutritional and medicinal ingredients.
Article
Chemistry, Analytical
Gabriela Islas, Jose A. Rodriguez, Irma Perez-Silva, Juan F. Flores-Aguilar, Eva M. Santos, Israel S. Ibarra
Summary: In this study, a technique involving dispersive solid-phase micro-extraction (DSP mu E) and graphene oxide (GO) particles with a higher oxygen content synthesized through the modification of the Hummers method was investigated for the removal of azo dyes from tap water samples. The optimal conditions for the removal of the dyes were determined, and the precision of the method was evaluated. The proposed method showed high efficiency in removing the azo dyes from the water samples.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL ANALYTICAL CHEMISTRY
(2023)
Article
Food Science & Technology
Guangfei Wei, Yichuan Liang, Guozhuang Zhang, Zhaoyu Zhang, Yongqing Zhang, Shilin Chen, Linlin Dong
Summary: This study investigated the presence of fungi on the surface of Polygonum multiflorum Thunb and found that it is easily contaminated by toxigenic fungi and mycotoxins, posing a health risk for consumers. The results also showed that sampling location and processing can influence the assembly and network complexity of the surface microbiome of Polygonum multiflorum Thunb.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Article
Food Science & Technology
Gabriele Busetta, Giuliana Garofalo, Salvatore Claps, Maria Teresa Sardina, Elena Franciosi, Antonio Alfonzo, Nicola Francesca, Giancarlo Moschetti, Luca Settanni, Raimondo Gaglio
Summary: This study evaluates the microbial diversity of wooden shelves used for the ripening of PDO Pecorino di Filiano and PGI Canestrato di Moliterno cheeses. The results show a strict correlation between the microbiota of wooden shelves and cheese rinds, indicating the positive role of wooden shelves in the cheese ripening process.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Article
Food Science & Technology
Massimo Todaro, Giuliana Garofalo, Gabriele Busetta, Riccardo Gannuscio, Ambra Rita Di Rosa, Maria Luisa Scatassa, Cinzia Cardamone, Isabella Mancuso, Elena Franciosi, Fatima Rando, Monica Agnolucci, Vincenzo Chiofalo, Raimondo Gaglio, Luca Settanni
Summary: This study aimed to reduce the production time of PDO Pecorino Siciliano cheese by comparing two cooking methods and assessing their impact on microbiological composition and lactic acid bacteria populations. The results showed that the different cooking methods did not affect the physicochemical characteristics of the cheese, and sensory evaluation confirmed that the modified production protocol did not significantly alter the product characteristics and overall acceptance. Therefore, cooking under hot water proved to be an effective way to reduce the transformation duration while maintaining the typicality of PDO Pecorino Siciliano cheese.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Article
Food Science & Technology
Yingying Sun, Yana Liu, Weiwei Zhou, Lele Shao, Han Wang, Yijie Zhao, Bo Zou, Xingmin Li, Ruitong Dai
Summary: The study evaluates the effect of Ohmic heating (OH) at various voltage gradients and water bath on microbial inactivation, physicochemical and sensory properties, and microbial flora of pasteurized milk. Results show that OH with higher voltage effectively inactivates microorganisms, slows down lipid oxidation, and maintains sensory quality and amino acids content of milk. The microbial community in milk treated with OH remains relatively stable during storage.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Article
Food Science & Technology
Minjin Oh, Ana Cevallos-Urena, Byoung Sik Kim
Summary: In this study, two bacteriophages, PECP14 and PECP20, infecting Escherichia coli O157:H7 were isolated and found to be stable under different environmental conditions. These phages exhibited specificity in targeting different host receptors and demonstrated the ability to infect other foodborne pathogens. The efficacy of phage treatment was demonstrated through rapid adsorption and bacterial lysis. Furthermore, purified endolysins derived from these phages showed remarkable bacteriolytic activity not only against E. coli O157:H7 but also against other pathogens. These findings suggest that these phages and their endolysins can be used for biological control of E. coli O157:H7 in various stages of agricultural product production.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Review
Food Science & Technology
Filippo Marzoli, Michela Bertola, Juliane Pinarelli Fazion, Giulia Cento, Pietro Antonelli, Beatrice Dolzan, Lisa Barco, Simone Belluco
Summary: Insects are a sustainable and protein-rich food source, and Salmonella is a common pathogen in insects. This systematic review aims to collect and evaluate existing studies on the occurrence of Salmonella in crickets and mealworms. Although the number of studies is limited and there are some limitations, this review highlights the importance of obtaining reliable data on the presence of Salmonella in insects, considering the growing market and increased farming scale.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Review
Food Science & Technology
Lucas Chikhi, Melanie Mancier, Hubert Brugere, Bertrand Lombard, Lyazrhi Faouzi, Laurent Guillier, Nathalie Gnanou Besse
Summary: Listeriosis is a severe foodborne disease with a high mortality rate, mainly caused by L. monocytogenes. The EU regulation sets qualitative or quantitative food safety criteria for L. monocytogenes and recommends the use of EN ISO 11290-1 as the reference detection method. Our study compares alternative certified methods for detecting L. monocytogenes, highlighting the importance of diversifying available detection methods.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Article
Food Science & Technology
Christina A. M. DeWitt, Kevin A. Nelson, Hyung Joo Kim, David H. Kingsley
Summary: High pressure processing (HPP) is a non-thermal method that can effectively inactivate pathogens. Research has shown that applying high pressure to thawed samples in an ultra-low temperature environment enhances the inactivation of norovirus.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Article
Food Science & Technology
Suzane Manzini, Thaina Valente Bertozzo, Isabella Neves Aires, Nassarah Jabur Lot Rodrigues, Amanda Bezerra Bertolini, Marcela Alexandrino, Jackieline Sampaio Steinle, Renata Pimentel Bandeira de Melo, Rinaldo Aparecido Mota, Maria Izabel Merino de Medeiros, Virginia Bodelao Richini-Pereira, Vera Claudia Lorenzetti Magalhaes Curci, Simone Baldini Lucheis
Summary: This study detected Toxoplasma gondii DNA in raw bovine milk samples from expansion tanks in small properties located in different cities of the Midwest region of Sa similar to o Paulo, Brazil using PCR. The results showed that the TgNP1 and TgNP2 genes were more efficient in detecting T. gondii DNA, highlighting the importance of raw bovine milk as a potential source of human infections caused by this parasite, and emphasizing the need for proper pasteurization and further understanding of the epidemiology of this protozoan.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Article
Food Science & Technology
Luca Bolzoni, Mauro Conter, Luca Lamperti, Erika Scaltriti, Marina Morganti, Antonio Poeta, Marco Vecchi, Silvia Paglioli, Alessandra Rampini, Paolo Ramoni, Daniela De Vita, Cristina Bacci, Martina Rega, Laura Andriani, Stefano Pongolini, Silvia Bonardi
Summary: This study assessed the role of equids as carriers of Salmonella and the occurrence of contaminated equid carcasses during the slaughter process. Salmonella was detected in a small percentage of equids' caecal contents, but not in carcass samples. Additionally, a genetic match was found between Salmonella strains from horse caecal contents and human cases, suggesting a link between horse meat consumption and salmonellosis.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Article
Food Science & Technology
Daria Nowinski, Tomasz Czapka, Irena Maliszewska
Summary: The effect of multiple sublethal doses of non-thermal plasma treatments on phenotypical changes and the reduction in phytopathogenicity of three species of fungi was investigated. The plasma damaged the surface of the fungal hyphae, decreased their dry biomass, and affected the activity of pectinase and xylanase. Furthermore, the plasma treatments increased the germination rate of artificially infected cucumber seeds and resulted in plants with higher biomass and longer roots and stems. However, the reduction in phytopathogenicity was not complete after the multiple plasma treatments.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)