Article
Chemistry, Analytical
Huan-Huan Zhu, Yuan Li, Li-Xia Wu, Ke-Sheng Wang, Yue Zhang, Qiang-Yuan Fan, Zun-Zhen Ming, Wei-Qin Chen, Wei-Wei Liu
Summary: Loop-mediated isothermal amplification (LAMP) is a promising diagnostic tool for genetic amplification, known for its speed, simplicity, efficiency, and sensitivity. This research introduces an internal heating method for LAMP using magnetic nanoparticles (MNPs) called nano-LAMP, which can detect low concentrations of HPV and has been validated in clinical samples.
Review
Microbiology
Cassandra Suther, Sloane Stoufer, Yanjiao Zhou, Matthew D. Moore
Summary: Foodborne and enteric viruses impose a significant burden on public health and economy globally. Real time PCR, although considered a gold standard, has limitations in terms of portability, sample processing, and time to result. Isothermal amplification methods have shown promise as portable, sensitive, and rapid alternatives to PCR for detecting these viruses.
FRONTIERS IN MICROBIOLOGY
(2022)
Review
Biotechnology & Applied Microbiology
Alangar I. Bhat, Rashid Aman, Magdy Mahfouz
Summary: Plant diseases caused by viruses have a significant impact on crop production and quality, resulting in substantial losses. The options for managing viruses are limited due to their nature as systemic obligate parasites. In this regard, sensitive, robust, and affordable diagnostic assays are needed to detect the presence of viruses in plant materials and prevent their spread. Traditional methods based on biological and physical properties have limitations in terms of sensitivity and time consumption. On the other hand, assays based on viral proteins and nucleic acids offer more specificity, sensitivity, and rapidity. However, they often require sophisticated equipment and technical skills in laboratory settings. Isothermal-based assays, such as LAMP and RPA, provide a simple and reliable alternative that does not require specialized equipment or skills. These assays can be performed using lateral flow devices, making them suitable for onsite detection or testing in the field. Isothermal amplification assays can also be combined with CRISPR/Cas technology to overcome non-specific amplification and cross-contamination issues. The collateral activity associated with some CRISPR/Cas systems has been successfully utilized for the visual detection of plant viruses.
PLANT BIOTECHNOLOGY JOURNAL
(2022)
Article
Agronomy
Martina Sanna, Davide Spadaro, Maria Lodovica Gullino, Monica Mezzalama
Summary: Fusarium fujikuroi, causing bakanae disease, is a significant seedborne pathogen of rice, and detecting it is crucial for seed certification and disease prevention. The study demonstrates the potential of LAMP technique for rapid and quantitative on-site detection of the pathogen.
Article
Plant Sciences
Brian Mueller, Carol L. Groves, Damon L. Smith
Summary: This study examined the Fusarium population on wheat in Wisconsin and the chemotype profile of isolates from 2016 and 2017. The majority of isolates were identified as the 15ADON chemotype, with 3ADON making up 9%. 3ADON isolates in Wisconsin exhibited higher sporulation capacity, growth rate, and DON production, but showed no significant difference in aggressiveness compared to 15ADON isolates.
Article
Biochemical Research Methods
Morteza Alipanah, Carlos Manzanas, Xin Hai, John A. Lednicky, Alberto Paniz-Mondolfi, J. Glenn Morris, Z. Hugh Fan
Summary: To differentiate Mayaro virus (MAYV) from other similar viruses diagnostically, a portable device integrating sample preparation with real-time, reverse-transcription, loop-mediated isothermal amplification (rRT-LAMP) has been developed. The device successfully detected MAYV without cross-reactions with CHIKV, DENV, or ZIKV. The platform allows rapid detection of MAYV in as short as 13 minutes, with a limit of detection as low as 10 genome equivalents per reaction.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Food Science & Technology
Alfonsina Fiore, Ida Treglia, Gianni Ciccaglioni, Marco Francesco Ortoffi, Antonietta Gattuso
Summary: Changing eating habits and rising demand of food have led to an increase in foodborne diseases, particularly in industrialized countries. Contaminated ready-to-eat food can transmit Listeria monocytogenes, a foodborne pathogen responsible for listeriosis. This study successfully applied a colorimetric Loop-mediated isothermal amplification (LAMP) assay to rapidly detect L. monocytogenes in experimentally contaminated RTE meat samples. The results showed high specificity and sensitivity compared to traditional methods, suggesting that colorimetric LAMP assays can be used as a screening tool for L. monocytogenes in ready-to-eat meat food.
Article
Environmental Sciences
Qiqi Xue, Lingwei Sheng, Junjie Guo, Minghui Zou, Qiao Teng, Sijia Xu, Yuanyuan Li, Jinhong Zhao
Summary: In this study, a loop-mediated isothermal amplification (LAMP) method was successfully established for the detection of the Dermatophagoides farinae allergen. This method showed higher sensitivity, specificity, and efficiency compared to conventional PCR. It did not cross-react with other arthropod species commonly found in indoor dust. This study provides a potential model for the rapid detection of allergens produced by other house dust mites in the future.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Chemistry, Analytical
Weiya Wang, Yuan Peng, Jin Wu, Man Zhang, Qiaofeng Li, Zunquan Zhao, Mingzhu Liu, Jiu Wang, Gaofang Cao, Jialei Bai, Zhixian Gao
Summary: A multi-step isothermal amplification fluorescence strategy was developed for quantitative detection of E2 at very low levels, with a good linear relationship between fluorescence value and E2 concentration. This method showed high sensitivity and specificity, providing a new possibility for highly sensitive detection of other small-molecule targets.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Yan Li, Hansol Kim, Yong Ju, Yeonkyung Park, Taejoon Kang, Dongeun Yong, Hyun Gyu Park
Summary: This study establishes a new method for detecting SARS-CoV-2 using self-priming hairpin-utilized isothermal amplification of the G-rich sequence. The method successfully detects the virus at low concentrations and demonstrates excellent clinical sensitivity and specificity in clinical samples.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Ane Rivas-Macho, Unai Eletxigerra, Ruth Diez-Ahedo, Angela Barros, Santos Merino, Felipe Goni-de-Cerio, Garbine Olabarria
Summary: An electrochemical sensor based on loop-mediated isothermal amplification (LAMP) technology was developed for the detection of SARS-CoV-2 virus in clinical samples. The sensor eliminates the need for DNA extraction by heating the sample, reducing the time required for each measurement.
Article
Biochemistry & Molecular Biology
Igor P. Oscorbin, Georgiy Yu. Shevelev, Ksenia A. Pronyaeva, Andrey A. Stepanov, Darya V. Shamovskaya, Olga V. Mishukova, Dmitrii V. Pyshnyi, Maksim L. Filipenko
Summary: Loop-mediated isothermal amplification (LAMP) is a stable nucleic acid amplification method that is resistant to DNA amplification inhibitors, allowing for multiplexing and suitable for SARS-CoV-2 viral RNA detection with high accuracy and short analysis time.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Food Science & Technology
Nor Asmara Tasrip, Mohd Nasir Mohd Desa, Nur Fadhilah Khairil Mokhtar, Nurhayatie Sajali, Amalia Mohd Hashim, Md. Eaqub Ali, Cheah Yoke Kqueen
Summary: The study successfully detected porcine DNA in gelatin products using porcine-specific LAMP technology, demonstrating excellent specificity, sensitivity, and rapidity.
JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE
(2021)
Article
Chemistry, Analytical
Wenjuan Wu, Congcong Yin, Aiqin Yue, Jingping Niu, Weijun Du, Dingbin Liu, Jinzhong Zhao
Summary: In this study, a loop-mediated isothermal amplification (LAMP) assay was developed for visual and rapid detection of the major prevalent SMV strain in China. The assay showed high analytical performance and potential for early detection of SMV-SC7-associated plant diseases.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Agriculture, Multidisciplinary
Valerie Nicaise, Sylvain Chereau, Laetitia Pinson-Gadais, Marie-Noelle Verdal-Bonnin, Christine Ducos, Melanie Jimenez, Cecile Coriou, Sylvie Bussiere, Thierry Robert, Christophe Nguyen, Florence Richard-Forget, Jean-Yves Cornu
Summary: This study investigates the interaction between cadmium (Cd) and Fusarium graminearum in durum wheat, finding that Cd reduces the concentration of deoxynivalenol (DON) in grains by enhancing plant resistance to the fungus, while F. graminearum infection increases the Cd concentration in grains by affecting nutrient allocation and grain sink strength.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Biotechnology & Applied Microbiology
Evelin F. Wigmann, Juergen Behr, Rudi F. Vogel, Ludwig Niessen
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2019)
Article
Food Science & Technology
Lisa M. Frisch, Ludwig Niessen
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2019)
Article
Mycology
Sebastian Ulrich, Ludwig Niessen, Julia Ekruth, Cornelius Schaefer, Florian Kaltner, Christoph Gottschalk
MYCOTOXIN RESEARCH
(2020)
Article
Food Science & Technology
Evelin F. Wigmann, Karsten Meyer, Eugenia Cendoya, Ronald Maul, Rudi F. Vogel, Ludwig Niessen
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2020)
Article
Biotechnology & Applied Microbiology
Stefan Schmideder, Henri Mueller, Lars Barthel, Tiaan Friedrich, Ludwig Niessen, Vera Meyer, Heiko Briesen
Summary: A generalized law for diffusive mass transport exists for filamentous fungal pellets, allowing for predictions and improvements in productivity of bioprocesses.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Article
Food Science & Technology
Magdalena A. Mann, Lisa M. Frisch, Rudi F. Vogel, Ludwig Niessen
Summary: Research indicated significant differences in PAU5 production among different yeast strains and under varied fermentation conditions, highlighting the potential impact on the gushing potential of sparkling wines.
FOOD RESEARCH INTERNATIONAL
(2021)
Article
Food Science & Technology
Lisa M. Frisch, Magdalena A. Mann, David N. Marek, Melanie Baudrexl, Rudi F. Vogel, Ludwig Niessen
Summary: The research found that Penicillium expansum plays a key role in inducing gushing in sparkling wine by secreting specific proteins, leading to significant economic losses for the beverage industry.
FOOD RESEARCH INTERNATIONAL
(2021)
Article
Biotechnology & Applied Microbiology
Lisa M. Frisch, Magdalena A. Mann, David N. Marek, Ludwig Niessen
Summary: Penicillium expansum, the main cause of Blue Mold Decay, is economically significant and poses health concerns due to the production of patulin. A LAMP assay was developed for specific detection, showing high specificity and sensitivity for P. expansum on various fruits. This assay serves as a promising tool for rapid and cost-efficient detection of the fungus in quality control applications in the food and beverage industry.
Article
Biochemical Research Methods
Johannes Koeck, Christoph Gottschalk, Sebastian Ulrich, Karin Schwaiger, Manfred Gareis, Ludwig Niessen
Summary: The study successfully developed a LAMP assay using sat14 gene-specific primers for selective identification of macrocyclic trichothecene producing S. chartarum isolates. The assay exhibited high specificity and sensitivity, with a detection limit of 0.635 pg/rxn and no cross-reaction with non-macrocyclic trichothecene producing strains or other fungal species. This cost-effective and rapid diagnostic tool has potential for practical applications.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Food Science & Technology
Evelin F. Tatsch, Karsten Meyer, Rudi F. Vogel, Ludwig Niessen
Summary: Fusarium verticillioides is a major producer of fumonisin, with its growth and fumonisin B-1 (FB1) production influenced by different carbon sources. The RT-LAMP assay successfully detected fum1 gene expression at early stages of F. verticillioides growth, allowing for prediction of FB1 formation.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Somayyeh Sedaghatjoo, Monika K. Forster, Ludwig Niessen, Petr Karlovsky, Berta Killermann, Wolfgang Maier
Summary: This study identified unique and conserved DNA regions specific to Tilletia controversa by comparing genomes of six Tilletia species, and developed a LAMP assay based on these regions. The assay showed high specificity, a low detection limit, and was validated in multiple laboratories.
SCIENTIFIC REPORTS
(2021)
Article
Biotechnology & Applied Microbiology
Monika K. Forster, Somayyeh Sedaghatjoo, Wolfgang Maier, Berta Killermann, Ludwig Niessen
Summary: The study assessed the ability of MALDI-TOF MS analysis to distinguish T. caries, T. laevis, and T. controversa, and explored its potential as an alternative method to morphology-based identification or germination tests. The results showed that MALDI-TOF MS can effectively differentiate T. controversa from the two causal agents of common bunt, but is unable to distinguish T. caries and T. laevis.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Microbiology
Sebastian Ulrich, Katharina Lang, Ludwig Niessen, Christiane Baschien, Robert Kosicki, Magdalena Twaruzek, Reinhard K. Straubinger, Frank Ebel
Summary: Stachybotrys chartarum is a mold that is often found in damp building materials or improperly stored animal forage. Exposure to the secondary metabolites of this mold can cause severe health effects. Through genetic analysis, we have proposed a model for the evolution of S. chartarum genotypes, suggesting that the H genotype is the oldest form, followed by the emergence of the S and A genotypes through gene loss and acquisition.
Article
Mycology
Ludwig Niessen, Marcus Fritze, Gudrun Wibbelt, Sebastien J. Puechmaille
Summary: Two independent LAMP assays based on acl1 and IGS genes were developed for the rapid and sensitive diagnosis of P. destructans. These assays could discriminate P. destructans from 159 tested species of filamentous fungi and yeasts. Moreover, the assays also worked with spores and mycelia of P. destructans without prior DNA extraction. The field-applicable version of the IGS-based LAMP assay and a protocol for preparation of fungal spores and mycelia were also developed.
Meeting Abstract
Dermatology
S. Ulrich, L. Niessen, F. Ebel
Article
Food Science & Technology
Guangfei Wei, Yichuan Liang, Guozhuang Zhang, Zhaoyu Zhang, Yongqing Zhang, Shilin Chen, Linlin Dong
Summary: This study investigated the presence of fungi on the surface of Polygonum multiflorum Thunb and found that it is easily contaminated by toxigenic fungi and mycotoxins, posing a health risk for consumers. The results also showed that sampling location and processing can influence the assembly and network complexity of the surface microbiome of Polygonum multiflorum Thunb.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Article
Food Science & Technology
Gabriele Busetta, Giuliana Garofalo, Salvatore Claps, Maria Teresa Sardina, Elena Franciosi, Antonio Alfonzo, Nicola Francesca, Giancarlo Moschetti, Luca Settanni, Raimondo Gaglio
Summary: This study evaluates the microbial diversity of wooden shelves used for the ripening of PDO Pecorino di Filiano and PGI Canestrato di Moliterno cheeses. The results show a strict correlation between the microbiota of wooden shelves and cheese rinds, indicating the positive role of wooden shelves in the cheese ripening process.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Article
Food Science & Technology
Massimo Todaro, Giuliana Garofalo, Gabriele Busetta, Riccardo Gannuscio, Ambra Rita Di Rosa, Maria Luisa Scatassa, Cinzia Cardamone, Isabella Mancuso, Elena Franciosi, Fatima Rando, Monica Agnolucci, Vincenzo Chiofalo, Raimondo Gaglio, Luca Settanni
Summary: This study aimed to reduce the production time of PDO Pecorino Siciliano cheese by comparing two cooking methods and assessing their impact on microbiological composition and lactic acid bacteria populations. The results showed that the different cooking methods did not affect the physicochemical characteristics of the cheese, and sensory evaluation confirmed that the modified production protocol did not significantly alter the product characteristics and overall acceptance. Therefore, cooking under hot water proved to be an effective way to reduce the transformation duration while maintaining the typicality of PDO Pecorino Siciliano cheese.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Article
Food Science & Technology
Yingying Sun, Yana Liu, Weiwei Zhou, Lele Shao, Han Wang, Yijie Zhao, Bo Zou, Xingmin Li, Ruitong Dai
Summary: The study evaluates the effect of Ohmic heating (OH) at various voltage gradients and water bath on microbial inactivation, physicochemical and sensory properties, and microbial flora of pasteurized milk. Results show that OH with higher voltage effectively inactivates microorganisms, slows down lipid oxidation, and maintains sensory quality and amino acids content of milk. The microbial community in milk treated with OH remains relatively stable during storage.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Article
Food Science & Technology
Minjin Oh, Ana Cevallos-Urena, Byoung Sik Kim
Summary: In this study, two bacteriophages, PECP14 and PECP20, infecting Escherichia coli O157:H7 were isolated and found to be stable under different environmental conditions. These phages exhibited specificity in targeting different host receptors and demonstrated the ability to infect other foodborne pathogens. The efficacy of phage treatment was demonstrated through rapid adsorption and bacterial lysis. Furthermore, purified endolysins derived from these phages showed remarkable bacteriolytic activity not only against E. coli O157:H7 but also against other pathogens. These findings suggest that these phages and their endolysins can be used for biological control of E. coli O157:H7 in various stages of agricultural product production.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Review
Food Science & Technology
Filippo Marzoli, Michela Bertola, Juliane Pinarelli Fazion, Giulia Cento, Pietro Antonelli, Beatrice Dolzan, Lisa Barco, Simone Belluco
Summary: Insects are a sustainable and protein-rich food source, and Salmonella is a common pathogen in insects. This systematic review aims to collect and evaluate existing studies on the occurrence of Salmonella in crickets and mealworms. Although the number of studies is limited and there are some limitations, this review highlights the importance of obtaining reliable data on the presence of Salmonella in insects, considering the growing market and increased farming scale.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Review
Food Science & Technology
Lucas Chikhi, Melanie Mancier, Hubert Brugere, Bertrand Lombard, Lyazrhi Faouzi, Laurent Guillier, Nathalie Gnanou Besse
Summary: Listeriosis is a severe foodborne disease with a high mortality rate, mainly caused by L. monocytogenes. The EU regulation sets qualitative or quantitative food safety criteria for L. monocytogenes and recommends the use of EN ISO 11290-1 as the reference detection method. Our study compares alternative certified methods for detecting L. monocytogenes, highlighting the importance of diversifying available detection methods.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Article
Food Science & Technology
Christina A. M. DeWitt, Kevin A. Nelson, Hyung Joo Kim, David H. Kingsley
Summary: High pressure processing (HPP) is a non-thermal method that can effectively inactivate pathogens. Research has shown that applying high pressure to thawed samples in an ultra-low temperature environment enhances the inactivation of norovirus.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Article
Food Science & Technology
Suzane Manzini, Thaina Valente Bertozzo, Isabella Neves Aires, Nassarah Jabur Lot Rodrigues, Amanda Bezerra Bertolini, Marcela Alexandrino, Jackieline Sampaio Steinle, Renata Pimentel Bandeira de Melo, Rinaldo Aparecido Mota, Maria Izabel Merino de Medeiros, Virginia Bodelao Richini-Pereira, Vera Claudia Lorenzetti Magalhaes Curci, Simone Baldini Lucheis
Summary: This study detected Toxoplasma gondii DNA in raw bovine milk samples from expansion tanks in small properties located in different cities of the Midwest region of Sa similar to o Paulo, Brazil using PCR. The results showed that the TgNP1 and TgNP2 genes were more efficient in detecting T. gondii DNA, highlighting the importance of raw bovine milk as a potential source of human infections caused by this parasite, and emphasizing the need for proper pasteurization and further understanding of the epidemiology of this protozoan.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Article
Food Science & Technology
Luca Bolzoni, Mauro Conter, Luca Lamperti, Erika Scaltriti, Marina Morganti, Antonio Poeta, Marco Vecchi, Silvia Paglioli, Alessandra Rampini, Paolo Ramoni, Daniela De Vita, Cristina Bacci, Martina Rega, Laura Andriani, Stefano Pongolini, Silvia Bonardi
Summary: This study assessed the role of equids as carriers of Salmonella and the occurrence of contaminated equid carcasses during the slaughter process. Salmonella was detected in a small percentage of equids' caecal contents, but not in carcass samples. Additionally, a genetic match was found between Salmonella strains from horse caecal contents and human cases, suggesting a link between horse meat consumption and salmonellosis.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Article
Food Science & Technology
Daria Nowinski, Tomasz Czapka, Irena Maliszewska
Summary: The effect of multiple sublethal doses of non-thermal plasma treatments on phenotypical changes and the reduction in phytopathogenicity of three species of fungi was investigated. The plasma damaged the surface of the fungal hyphae, decreased their dry biomass, and affected the activity of pectinase and xylanase. Furthermore, the plasma treatments increased the germination rate of artificially infected cucumber seeds and resulted in plants with higher biomass and longer roots and stems. However, the reduction in phytopathogenicity was not complete after the multiple plasma treatments.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)