Article
Food Science & Technology
Vincent M. Blaschke, Thao Uyen Tran, Mohammad Naneh, Jutta Zagon, Matthias Winkel
Summary: In this study, a highly specific duplex PCR method was developed for the detection of most commercially relevant crustaceans in food. The limit of detection was 1 pg of crustacean DNA or 10 ppm of crustacean sample. Cross-testing with various plant and animal species ensured practical applicability, and a robustness test confirmed the practical use of the protocol.
Article
Agriculture, Multidisciplinary
Vincent Blaschke, Alea Berten, Heike Sprenger, Jutta Zagon, Matthias Winkel
Summary: In this study, highly specific real-time PCR methods were developed for the comprehensive detection of all commercially relevant cephalopod species and the gastropod families Helicidae, Buccinidae, and Muricidae in food matrices. The specificity of the systems was demonstrated by cross-testing over 100 animal and plant species. The robustness and practical applicability of the protocol were confirmed through testing multiple parameters and cooking and autoclaving of samples.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Genetics & Heredity
Nicol Janecko, Samuel J. Bloomfield, Raphaelle Palau, Alison E. Mather
Summary: The consumption of prawns as a protein source is increasing globally, with significant levels of Vibrio contamination and antimicrobial resistance genes found in prawn samples. The study highlights the diversity of Vibrio species and prevalence of resistance genes in prawns available at retail.
MICROBIAL GENOMICS
(2021)
Article
Geosciences, Multidisciplinary
Lars Moeller, Bernd Kreikemeyer, Guennar Gerdts, Gunter Jost, Matthias Labrenz
Summary: This study identified fish, especially in the southern Baltic Sea, as potential winter reservoirs for Vibrio spp., including pathogenic species. The invasive species round goby has the capacity to carry a comparable number of Vibrio spp. cells like water and sediment, making it a potential hot spot for pathogenic Vibrio species.
JOURNAL OF MARINE SYSTEMS
(2021)
Article
Chemistry, Analytical
Ritong Sun, Jiao Chen, Yingeng Wang, Zheng Zhang, Yong Li, Fengmei Li, Cuiping Ma, Qingxia Han, Yanjing Shi
Summary: A highly sensitive and specific real-time narrow thermal-cycling amplification detection method based on accelerated strand exchange amplification (ASEA) was developed for the detection of Vibrio parahaemolyticus. The method can detect low concentrations of Vibrio parahaemolyticus in cultured samples and artificially spiked scallop meat within a short time. It is a useful tool for rapid detection of Vibrio parahaemolyticus in seafood samples.
ANALYTICAL METHODS
(2023)
Article
Veterinary Sciences
Marika Grillini, Paola Beraldo, Antonio Frangipane di Regalbono, Giorgia Dotto, Cinzia Tessarin, Giovanni Franzo, Erica Marchiori, David Modry, Giulia Simonato
Summary: This study developed a fast and cost-saving real-time PCR method to detect Cytauxzoon and Hepatozoon parasites in felids in northeastern Italy. The results showed that Hepatozoon felis was commonly found in domestic cats, while Hepatozoon silvestris and Cytauxzoon europaeus were mainly found in wildcats.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Chemistry, Multidisciplinary
Yue Zhai, Xiangjun Meng, Li Li, Yushen Liu, Kun Xu, Chao Zhao, Juan Wang, Xiuling Song, Juan Li, Minghua Jin
Summary: This study presents a rapid and sensitive detection method for Vibrio parahaemolyticus by integrating magnetic nanobeads and quantum dots technology. The detection combines immunoseparation and immunofluorescence to achieve efficient detection of Vibrio parahaemolyticus, showing satisfactory results in real food samples.
Article
Food Science & Technology
A. G. Mudadu, S. Salza, R. Melillo, L. Mara, G. Piras, C. Spanu, G. Terrosu, A. Fadda, S. Virgilio, T. Tedde
Summary: This study assessed the prevalence of Arcobacter spp. and the presence of virulence genes in edible bivalve molluscs from production farms and retail markets in Sardinia, Italy. The findings indicate a potential health risk associated with the consumption of these shellfish, especially when eaten raw or undercooked, highlighting the need for specific and continuous surveillance programs to ensure food safety.
Article
Microbiology
Yong Yan, Li Zhan, Guoying Zhu, Junyan Zhang, Ping Li, Lixia Chen, Peiyan He, Jianyong Luo, Zhongwen Chen
Summary: A novel multiplex assay for rapid detection of Vibrio cholerae serogroup and cholera toxin toxigenicity was developed. The assay showed good diagnostic sensitivities and is suitable for rapid identification and screening of large specimens.
Article
Immunology
Zhenpeng Li, Hongxia Guan, Wei Wang, He Gao, Weihong Feng, Jie Li, Baowei Diao, Hongqun Zhao, Biao Kan, Jingyun Zhang
Summary: A multiplex TaqMan-based real-time PCR assay was developed to detect and differentiate Vibrio cholerae and Vibrio parahaemolyticus directly from human fecal specimens with high sensitivity and specificity. The BD MAX assay showed high overall agreement with conventional real-time PCR, making it an effective detection method for these pathogens.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Veterinary Sciences
A-Tai Truong, Bo-Ram Yun, Mi-Sun Yoo, Jiyeon Lim, Subin Min, Soon-Seek Yoon, Young-Min Yun, Jong-Taek Kim, Yun Sang Cho
Summary: The study evaluated a molecular tool, ultra-rapid real-time PCR, for rapid and accurate detection of Rickettsia from ticks collected in Gangwon and Jeju province in South Korea, providing useful information on the distribution of Rickettsia in these regions.
BMC VETERINARY RESEARCH
(2022)
Article
Engineering, Environmental
Jamie Harrison, Kathryn Nelson, Helen Morcrette, Cyril Morcrette, Joanne Preston, Luke Helmer, Richard W. Titball, Clive S. Butler, Sariqa Wagley
Summary: Rising sea-surface temperature has led to an increase in the prevalence of Vibrio species in marine environments, particularly in temperate regions. This has resulted in a surge of Vibriosis infections worldwide. Using sea-surface temperature data around the English and Welsh coastlines, this study identifies locations where conditions for the presence and growth of Vibrio species are favorable. Shellfish samples collected from locations with increasing sea-surface temperature tested positive for the presence of Vibrio species, including aquaculture pathogens and human pathogenic species not previously reported in UK waters.
Article
Chemistry, Multidisciplinary
Bong Kyun Kim, Sang-A Lee, Minju Park, Eui Ju Jeon, Mi Jung Kim, Jung Min Kim, Heesuk Kim, Seungwon Jung, Sang Kyung Kim
Summary: This article introduces a photonic PCR platform using hydrogel microparticles, which utilizes photothermal nanomaterials as heating elements for rapid and portable POCT. The researchers successfully demonstrate the ability to perform multiplex assays by loading multiple encoded pPIN microparticles in a single reaction.
Article
Agriculture, Dairy & Animal Science
Dimitris Dimzas, Taxiarchis Chassalevris, Zanda Ozolina, Chrysostomos Dovas, Anastasia Diakou
Summary: This study conducted the first investigation on the presence of parasites in wild boar meat in Greece, and it was found that none of the samples tested positive for Trichinella spp. or Alaria spp. A novel molecular method for detecting Alaria spp. was developed, which could be a useful complementary diagnostic tool in surveillance for this parasite.
Article
Food Science & Technology
Seul-Ki Park, Kyung-Jin Cho, Du-Min Jo, Young-Mog Kim
Summary: This study presents a new approach for detecting pathogenic Vibrio spp. in contaminated seafood, which combines PCR with a simplified DNA extraction process and pre-culturing step. The method has been validated and shown to be efficient, time-saving, and highly sensitive, offering a potential alternative for improving safety measures in the food industry.
Article
Food Science & Technology
Guangfei Wei, Yichuan Liang, Guozhuang Zhang, Zhaoyu Zhang, Yongqing Zhang, Shilin Chen, Linlin Dong
Summary: This study investigated the presence of fungi on the surface of Polygonum multiflorum Thunb and found that it is easily contaminated by toxigenic fungi and mycotoxins, posing a health risk for consumers. The results also showed that sampling location and processing can influence the assembly and network complexity of the surface microbiome of Polygonum multiflorum Thunb.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Article
Food Science & Technology
Gabriele Busetta, Giuliana Garofalo, Salvatore Claps, Maria Teresa Sardina, Elena Franciosi, Antonio Alfonzo, Nicola Francesca, Giancarlo Moschetti, Luca Settanni, Raimondo Gaglio
Summary: This study evaluates the microbial diversity of wooden shelves used for the ripening of PDO Pecorino di Filiano and PGI Canestrato di Moliterno cheeses. The results show a strict correlation between the microbiota of wooden shelves and cheese rinds, indicating the positive role of wooden shelves in the cheese ripening process.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Article
Food Science & Technology
Massimo Todaro, Giuliana Garofalo, Gabriele Busetta, Riccardo Gannuscio, Ambra Rita Di Rosa, Maria Luisa Scatassa, Cinzia Cardamone, Isabella Mancuso, Elena Franciosi, Fatima Rando, Monica Agnolucci, Vincenzo Chiofalo, Raimondo Gaglio, Luca Settanni
Summary: This study aimed to reduce the production time of PDO Pecorino Siciliano cheese by comparing two cooking methods and assessing their impact on microbiological composition and lactic acid bacteria populations. The results showed that the different cooking methods did not affect the physicochemical characteristics of the cheese, and sensory evaluation confirmed that the modified production protocol did not significantly alter the product characteristics and overall acceptance. Therefore, cooking under hot water proved to be an effective way to reduce the transformation duration while maintaining the typicality of PDO Pecorino Siciliano cheese.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Article
Food Science & Technology
Yingying Sun, Yana Liu, Weiwei Zhou, Lele Shao, Han Wang, Yijie Zhao, Bo Zou, Xingmin Li, Ruitong Dai
Summary: The study evaluates the effect of Ohmic heating (OH) at various voltage gradients and water bath on microbial inactivation, physicochemical and sensory properties, and microbial flora of pasteurized milk. Results show that OH with higher voltage effectively inactivates microorganisms, slows down lipid oxidation, and maintains sensory quality and amino acids content of milk. The microbial community in milk treated with OH remains relatively stable during storage.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Article
Food Science & Technology
Minjin Oh, Ana Cevallos-Urena, Byoung Sik Kim
Summary: In this study, two bacteriophages, PECP14 and PECP20, infecting Escherichia coli O157:H7 were isolated and found to be stable under different environmental conditions. These phages exhibited specificity in targeting different host receptors and demonstrated the ability to infect other foodborne pathogens. The efficacy of phage treatment was demonstrated through rapid adsorption and bacterial lysis. Furthermore, purified endolysins derived from these phages showed remarkable bacteriolytic activity not only against E. coli O157:H7 but also against other pathogens. These findings suggest that these phages and their endolysins can be used for biological control of E. coli O157:H7 in various stages of agricultural product production.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Review
Food Science & Technology
Filippo Marzoli, Michela Bertola, Juliane Pinarelli Fazion, Giulia Cento, Pietro Antonelli, Beatrice Dolzan, Lisa Barco, Simone Belluco
Summary: Insects are a sustainable and protein-rich food source, and Salmonella is a common pathogen in insects. This systematic review aims to collect and evaluate existing studies on the occurrence of Salmonella in crickets and mealworms. Although the number of studies is limited and there are some limitations, this review highlights the importance of obtaining reliable data on the presence of Salmonella in insects, considering the growing market and increased farming scale.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Review
Food Science & Technology
Lucas Chikhi, Melanie Mancier, Hubert Brugere, Bertrand Lombard, Lyazrhi Faouzi, Laurent Guillier, Nathalie Gnanou Besse
Summary: Listeriosis is a severe foodborne disease with a high mortality rate, mainly caused by L. monocytogenes. The EU regulation sets qualitative or quantitative food safety criteria for L. monocytogenes and recommends the use of EN ISO 11290-1 as the reference detection method. Our study compares alternative certified methods for detecting L. monocytogenes, highlighting the importance of diversifying available detection methods.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Article
Food Science & Technology
Christina A. M. DeWitt, Kevin A. Nelson, Hyung Joo Kim, David H. Kingsley
Summary: High pressure processing (HPP) is a non-thermal method that can effectively inactivate pathogens. Research has shown that applying high pressure to thawed samples in an ultra-low temperature environment enhances the inactivation of norovirus.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Article
Food Science & Technology
Suzane Manzini, Thaina Valente Bertozzo, Isabella Neves Aires, Nassarah Jabur Lot Rodrigues, Amanda Bezerra Bertolini, Marcela Alexandrino, Jackieline Sampaio Steinle, Renata Pimentel Bandeira de Melo, Rinaldo Aparecido Mota, Maria Izabel Merino de Medeiros, Virginia Bodelao Richini-Pereira, Vera Claudia Lorenzetti Magalhaes Curci, Simone Baldini Lucheis
Summary: This study detected Toxoplasma gondii DNA in raw bovine milk samples from expansion tanks in small properties located in different cities of the Midwest region of Sa similar to o Paulo, Brazil using PCR. The results showed that the TgNP1 and TgNP2 genes were more efficient in detecting T. gondii DNA, highlighting the importance of raw bovine milk as a potential source of human infections caused by this parasite, and emphasizing the need for proper pasteurization and further understanding of the epidemiology of this protozoan.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Article
Food Science & Technology
Luca Bolzoni, Mauro Conter, Luca Lamperti, Erika Scaltriti, Marina Morganti, Antonio Poeta, Marco Vecchi, Silvia Paglioli, Alessandra Rampini, Paolo Ramoni, Daniela De Vita, Cristina Bacci, Martina Rega, Laura Andriani, Stefano Pongolini, Silvia Bonardi
Summary: This study assessed the role of equids as carriers of Salmonella and the occurrence of contaminated equid carcasses during the slaughter process. Salmonella was detected in a small percentage of equids' caecal contents, but not in carcass samples. Additionally, a genetic match was found between Salmonella strains from horse caecal contents and human cases, suggesting a link between horse meat consumption and salmonellosis.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Article
Food Science & Technology
Daria Nowinski, Tomasz Czapka, Irena Maliszewska
Summary: The effect of multiple sublethal doses of non-thermal plasma treatments on phenotypical changes and the reduction in phytopathogenicity of three species of fungi was investigated. The plasma damaged the surface of the fungal hyphae, decreased their dry biomass, and affected the activity of pectinase and xylanase. Furthermore, the plasma treatments increased the germination rate of artificially infected cucumber seeds and resulted in plants with higher biomass and longer roots and stems. However, the reduction in phytopathogenicity was not complete after the multiple plasma treatments.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
