4.7 Article

Genetic diversity and physiological traits of Brettanomyces bruxellensis strains isolated from Tuscan Sangiovese wines

Journal

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
Volume 130, Issue 3, Pages 238-244

Publisher

ELSEVIER
DOI: 10.1016/j.ijfoodmicro.2009.01.025

Keywords

Brettanomyces bruxellensis; Sangiovese; Random amplified polymorphism DNA; Mitochondrial DNA restriction analysis; Volatile phenols; Biogenic amines

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Eighty four isolates of Brettanomyces bruxellensis, were collected during fermentation of Sangiovese grapes in several Tuscan wineries and characterized by restriction analysis of 5.85-ITS and species-specific PCR. The isolates were Subsequently analysed, at strain level, by the combined use of the RAPD-PCR assay with primer OPA-02 and the mtDNA restriction analysis with the Hinfl endonuclease. This approach showed a high degree of polymorphism and allowed to identify seven haplotypes, one of them being the most represented and widely distributed (72 isolates, 85.7%). Physiological traits of the yeasts were investigated under a wine model condition. Haplotypes clustered into two groups according to their growth rates and kinetics of production of 4-ethylphenol and 4-ethylguaiacol. Hexylamine was the biogenic amine most produced (up to 3.92 mg l(-1)), followed by putrescine and phenylethylamine. Formation of octapamine was detected by some haplotypes, for the first time. (C) 2009 Elsevier B.V. All rights reserved.

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