Article
Food Science & Technology
Kungnang Bunsroem, Witoon Prinyawiwatkul, Siwatt Thaiudom
Summary: This study evaluated the susceptibility of whey proteins to enzyme hydrolysis and the antidiabetic properties of protein hydrolysates. The results showed that alpha-LA hydrolysate was more effective than beta-LG hydrolysate in inhibiting DPP-W activity and increasing GLP-1 levels. Heat treatment at 75 degrees C had the greatest impact on the antidiabetic properties of the protein hydrolysates.
Article
Food Science & Technology
Maria Romo, Massimo Castellari, Dinar Fartdinov, Xavier Felipe
Summary: In this study, high-pressure processing was used to remove beta-Lactoglobulin from bovine native whey concentrate and generate an enriched alpha-Lactalbumin fraction. The optimal conditions for alpha-Lactalbumin yield and purification degree were determined. The results showed that high-pressure processing could improve the precipitation of beta-Lactoglobulin and the purification of alpha-Lactalbumin. Further characterization and application of membrane concentration and high-pressure processing are recommended for the valorization of cheese whey.
Article
Agriculture, Dairy & Animal Science
Brandon Carter, Larissa DiMarzo, Joice Pranata, David M. Barbano, MaryAnne Drake
Summary: The study aimed to measure whey protein removal from sweet whey using polymeric microfiltration membranes and compared the performance with ceramic membranes. Results showed that polymeric membranes achieved lower yield of whey protein isolate and higher yield of whey protein phospholipid concentrate compared to ceramic membranes, impacting total gross revenue and the ratio of market price between the two products. Economic engineering study is needed to determine the long-term processing costs and net revenue under various market conditions.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Agriculture, Dairy & Animal Science
Kamila Puppel, Patrycja Staniszewska, Marcin Golebiewski, Jan Slosarz, Grzegorz Grodkowski, Pawel Solarczyk, Malgorzata Kunowska-Slosarz, Piotr Kostusiak, Beata Kuczynska, Tomasz Przysucha
Summary: This study demonstrates a relationship between whey protein concentrations and blood BHBA levels, serving as markers for diagnosing metabolic diseases. Differences in alpha-lactoalbumin and beta-lactoglobulin concentrations in milk were observed based on varying BHBA levels in cows.
Article
Chemistry, Multidisciplinary
Rosalinda Mazzei, Anna Maria Szymczak, Enrico Drioli, Mohamed Al-Fageeh, Mohammed A. Aljohi, Lidietta Giorno
Summary: This study systematically investigated the separation of alpha-lactalbumin and beta-lactoglobulin using charged ultrafiltration membranes, achieving high purity (95%) and recovery factor (80%) in a single step. By operating far from the proteins' isoelectric point, protein aggregation and membrane fouling were minimized.
APPLIED SCIENCES-BASEL
(2021)
Article
Chemistry, Applied
Clara Fucinos, Natalia Estevez, Lorenzo Pastrana, Clara A. Tovar, Maria Luisa Rua
Summary: The bioactivity of α-lactalbumin was significantly improved by the synthesis of nanotubes, with higher ACE-inhibitory and antioxidant activity. Different ion ratios during nanotube synthesis altered the microstructure of α-lactalbumin nanotubes, resulting in varied rheological properties and structural regeneration capabilities.
FOOD HYDROCOLLOIDS
(2021)
Article
Food Science & Technology
Ruiyang Ma, Na Miao, Qingyi Zhang, Shujuan Jiang, Xinling Li, Lei Chi, Yue Li, Guangqing Mu, Xuemei Zhu
Summary: The experiment used a top-down method to quickly and efficiently screen strains with low allergenic potential and prepared fermented milk. Through direct screening of traditional fermented dairy products from Xinjiang, the BZ-21 sample with the lowest antigenicity was selected and 13 strains were isolated from it. These strains were safe and the fermented milk prepared under optimized fermentation conditions showed reduced antigenicity and IgE-binding capacity. The top-down screening method used in this experiment was effective and these 13 strains will be potential probiotics in the development of low-allergenic dairy products.
Article
Agriculture, Dairy & Animal Science
Brandon Carter, Larissa DiMarzo, Joice Pranata, David M. Barbano, MaryAnne Drake
Summary: The study aimed to measure the removal of whey protein using 0.1-micron ceramic microfiltration membranes at 50 degrees Celsius. Results showed that most total nitrogen passed through the membrane into the permeate, lactoferrin did not pass through, while other components such as IgG, bovine serum albumen, glycomacropeptide, and casein proteolysis products did pass into the permeate.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Food Science & Technology
Maria Romo, Massimo Castellari, Ricard Bou, Pere Gou, Xavier Felipe
Summary: Whey from goat and sheep are being studied for their nutritional properties, but the presence of beta-Lg in these whey sources may cause allergies in infants. Therefore, researchers are interested in developing whey ingredients with higher alpha-La/beta-Lg ratios. This study investigated the effect of high-pressure processing on goat and sheep native whey concentrates (NWC) to obtain alpha-La-enriched fractions. The results showed that high-pressure processing reduced the concentration of beta-Lg in NWC, while alpha-La concentration remained unchanged.
Article
Agriculture, Dairy & Animal Science
E. Reale, S. Govindasamy-Lucey, Y. Lu, M. E. Johnson, J. J. Jaeggi, M. Molitor, J. A. Lucey
Summary: An increasing number of cheese-making companies are using high-protein milks to increase cheese yield. This study explored the impact of reducing whey protein (WP) levels in concentrated cheese milk on cheese characteristics. The results showed that reducing WP levels did not affect the pH, sensory attributes, or insoluble calcium content of cheese, but it did lower the protein hydrolysis and hardness and melting point of the cheese.
JOURNAL OF DAIRY SCIENCE
(2022)
Article
Engineering, Chemical
Marlen Gonzalez-Amado, Ana P. M. Tavares, Mara G. Freire, Ana Soto, Oscar Rodriguez
Summary: This study investigated the use of Aqueous Two-Phase Systems (ATPS) for the recovery of lactose, BSA, beta-lactoglobulin and alpha-lactalbumin from cheese whey. Different ATPS were studied for partitioning the selected solutes, showing efficient recovery of proteins and lactose under optimized conditions. This polymer/salt ATPS method could be a scalable and cost-effective separation strategy for cheese whey and related wastes.
SEPARATION AND PURIFICATION TECHNOLOGY
(2021)
Review
Food Science & Technology
Jianxin Xiao, Jiaying Ma, Muhammad Zahoor Khan, Gibson Maswayi Alugongo, Tianyu Chen, Shuai Liu, Shengli Li, Zhijun Cao
Summary: Whey protein components have anticancer attributes and can reduce the risk of colorectal cancer. They have anti-inflammatory and antioxidant abilities and can induce apoptosis, inhibit tumor cell proliferation, and impede metastasis. Studies have shown the importance of incorporating whey protein components into a balanced daily diet for the prevention and treatment of colorectal cancer.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2023)
Article
Food Science & Technology
Serena Boscaini, Peter Skuse, Kanishka N. Nilaweera, John F. Cryan, Paul D. Cotter
Summary: This review provides an overview of the beneficial effects of whey protein (WP) and its components on human health, particularly in controlling obesity and its comorbidities. WP consumption positively impacts energy balance and obesity-related parameters, primarily through modulation in adipose tissue metabolism and changes that occur in the gastrointestinal tract. Furthermore, WP also has a positive impact on gut microbiota, mood, and behavior. This review emphasizes the importance of WP supplementation in preventing or treating obesity and highlights areas for further research.
TRENDS IN FOOD SCIENCE & TECHNOLOGY
(2023)
Article
Engineering, Chemical
Malou Warncke, Ingrun Kieferle, Thanh Mai Nguyen, Ulrich Kulozik
Summary: The research investigated the mechanisms responsible for viscosity changes in milk protein concentrates based on total protein concentration, casein/whey protein ratio, and heating, showing that whey protein depletion was key in impacting viscosity, polydispersity, and whey protein denaturation.
JOURNAL OF FOOD ENGINEERING
(2022)
Review
Chemistry, Analytical
Jelena Radosavljevic, Dragana Stanic-Vucinic, Marija Stojadinovic, Mirjana Radomirovic, Ana Simovic, Milica Radibratovic, Tanja Cirkovic Velickovic
Summary: Whey protein, an important source of nutrition and biotechnology, is considered a by-product of the dairy industry due to low economic value. The challenge in whey protein separation lies in low concentration and similar properties. Ion exchange techniques are widely used for separation, and recent research on ion exchange membranes shows promise for industrial application. Adsorption-based methodologies targeting protein structure differences are anticipated to further improve whey protein separation.
CURRENT ANALYTICAL CHEMISTRY
(2022)
Article
Environmental Sciences
Bianca Santinon Scopel, Danielle Restelatto, Camila Baldasso, Aline Dettmer, Ruth Marlene Campomanes Santana
ENVIRONMENTAL TECHNOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Caroline Martins Machado, Patricia Benelli, Isabel Cristina Tessaro
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2020)
Article
Materials Science, Biomaterials
Patricia Hubner, Nicoly Donati, Luci Kelin de Menezes Quines, Isabel Cristina Tessaro, Nilson Romeu Marcilio
MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
(2020)
Article
Polymer Science
Erli Jose Padilha Junior, Paula Bettio Staudt, Isabel Cristina Tessaro, Nilo Sergio Medeiros Cardozo
Article
Polymer Science
Julia M. Frick Pavoni, Nicole Z. dos Santos, Izabelly C. May, Liliane D. Pollo, Isabel C. Tessaro
Summary: The properties of chitosan films are significantly affected by the type of acid and glutaraldehyde crosslinking, which reduces the swelling degree and increases fragility of the films. Crosslinking does not completely prevent the biodegradation of the films but requires a longer time. Ecotoxicity analysis suggests that the films can be composted.
Article
Materials Science, Ceramics
Melissa R. de la Rocha, Mirella Virginie, Andrei Khodakov, Liliane D. Pollo, Nilson R. Marcilio, Isabel C. Tessaro
Summary: The addition of coal fly ash to alumina-based membranes increases porosity and water permeability while reducing mechanical strength and linear shrinkage. These membranes have high cornstarch retention, making them cost-effective and suitable for applications such as wastewater treatment.
CERAMICS INTERNATIONAL
(2021)
Article
Engineering, Biomedical
Patricia Hubner, Nilson Romeu Marcilio, Isabel Cristina Tessaro
Summary: Hydrogel films based on gelatin and PVA were developed and optimized using a central composite design method, showing characteristics suitable for biomedical applications such as wound dressings. The optimized film had good mechanical resistance, elasticity, and permeability to oxygen, making it a promising candidate for use in biomedical settings.
JOURNAL OF BIOMATERIALS APPLICATIONS
(2021)
Article
Chemistry, Multidisciplinary
Ana Katiuce Fellenberg, Claudia Leites Luchese, Nilson Romeu Marcilio, Isabel Cristina Tessaro
Summary: This research focused on developing carbon membranes using poly(ether sulfone) as a polymeric precursor and DMSO as a solvent, formed through dip-coating technique on alumina ceramic tubes with pyrolysis conducted at 700 degrees C. The obtained carbon membranes showed high thermal stability and microporous characteristics, suitable for gas separation processes.
KOREAN JOURNAL OF CHEMICAL ENGINEERING
(2021)
Article
Engineering, Chemical
Vinicius da Costa Avila, Isabel Cristina Tessaro, Nilo Sergio Medeiros Cardozo
Summary: Accurate spatial discretization error estimates are crucial for certain CFD applications. This study evaluated the suitability of three error estimators in simulations of a benchmark bubble column and found that EH-LS was the only method suitable for both MixtM and hTFM. DTS resulted in inaccurate estimates for coarse meshes.
CHEMICAL ENGINEERING SCIENCE
(2021)
Article
Chemistry, Applied
Juliana Both Engel, Claudia Leites Luchese, Isabel Cristina Tessaro
Summary: This study explored the use of cassava residues in producing foams, finding that pre-gelatinization can enhance the mechanical strength and elongation of biocomposites, while cassava inner bark can be used to create packaging materials with lower water absorption capacity. Additionally, skipping the pre-gelatinization step can increase production speed and reduce costs.
FOOD HYDROCOLLOIDS
(2021)
Article
Biochemistry & Molecular Biology
Andressa de Espindola Sobczyk, Claudia Leites Luchese, Debora Jung Luvizetto Faccin, Isabel Cristina Tessaro
Summary: Biopolymers like chitosan and alginate have been used in biomedical applications, especially in wound dressings as substitutes for synthetic polymers. The study compared the influence of antimicrobial agent incorporation form on the properties of chitosan/alginate-based films, finding that films containing oregano essential oil or ground oregano leaves showed promise for wound dressing applications with good physicochemical properties and antimicrobial activity.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biochemistry & Molecular Biology
Claudia Leites Luchese, Raul Barth Rodrigues, Isabel Cristina Tessaro
Summary: The development of starch-based films has shown the potential for creating transparent, non-toxic, biodegradable materials with good gas barrier properties. However, the use of starch in packaging should be avoided due to world hunger-related problems. Utilizing residual starches can reduce production costs and promote the development of innovative biomaterials. The addition of gelatinized starch from agro-industrial residues in bioplastic production has shown a decrease in tensile strength and elastic modulus values, but no significant impact on elongation percentage values.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Materials Science, Multidisciplinary
Janice B. S. Hamm, Alan Ambrosi, Liliane D. Pollo, Nilson R. Marcilio, Isabel Cristina Tessaro
Summary: This paper presents a new approach for obtaining a thin polymeric film on porous supports using the dip-coating/phase inversion technique. The method, known as the wet-dry process, effectively minimizes intrusion into the support pores. Morphology was analyzed using scanning electron microscopy to confirm the efficacy of the new technique.
MATERIALS CHEMISTRY AND PHYSICS
(2021)
Article
Chemistry, Applied
E. Baggio, B. S. Scopel, D. D. C. Krein, C. V. T. Rigueto, F. C. Kovalski, C. Baldasso, A. Dettmer
Summary: The production of biodegradable polymers is a promising alternative to reduce residues and environmental pollution. Tannery wastes can be used for gelatin extraction and film production, with enzyme crosslinking improving properties such as water permeability and solubility. The enzyme transglutaminase can significantly affect the properties of gelatin films and their rate of soil degradation.
JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION
(2021)
Article
Engineering, Environmental
Jordana Corralo Spada, Sabrina Ferreira Seibert, Isabel Cristina Tessaro
Summary: Composites with high rice husk content and coated with PLA and PBAT were evaluated for their properties. The double coating of PLA resulted in a more uniform surface, decreased water absorption, and improved hydrophilicity.
JOURNAL OF POLYMERS AND THE ENVIRONMENT
(2021)