Article
Immunology
Jeremiah L. Oyer, Tayler J. Croom-Perez, Thomas A. Dieffenthaller, Liza D. Robles-Carillo, Sarah B. Gitto, Deborah A. Altomare, Alicja J. Copik
Summary: This study evaluated the impact of cryopreservation on PM21-NK cells and found that PM21-NK cells can be efficiently cryopreserved and recovered without substantial loss of viability or activity, while retaining effector function both in vitro and in vivo.
FRONTIERS IN IMMUNOLOGY
(2022)
Letter
Oncology
Hao Zhang, Yongxian Hu, Mi Shao, Xinyi Teng, Penglei Jiang, Xiujian Wang, Hui Wang, Jiazhen Cui, Jian Yu, Zuyu Liang, Lijuan Ding, Yingli Han, Jieping Wei, Yulin Xu, Xiaoqing Li, Wei Shan, Jimin Shi, Yi Luo, Pengxu Qian, He Huang
Summary: Dasatinib has shown promising results in preventing or reversing differentiation and exhaustion of CAR-T cells, significantly enhancing therapeutic efficacy and in vivo persistence.
JOURNAL OF HEMATOLOGY & ONCOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Chulhwan Kwak, Cheolhun Park, Minjeong Ko, Chun Young Im, Heegyum Moon, Young-Hoon Park, So Young Kim, Seungyeon Lee, Myeong-Gyun Kang, Ho Jeong Kwon, Eunmi Hong, Jeong Kon Seo, Hyun-Woo Rhee
Summary: Direct identification of drug-target proteins can provide valuable insights for disease diagnosis, prevention, and drug development. In this study, researchers developed a target-ID system called PROCID, which combines a direct analysis workflow of proximity-labeled proteins with the HaloTag system. They successfully identified known and unknown drug-interacting proteins in live cells, providing a robust method to explore the mode of action of drugs.
CELL CHEMICAL BIOLOGY
(2022)
Article
Oncology
Hyunsoo Cho, Kyung Hwan Kim, Hoyoung Lee, Chang Gon Kim, Haerim Chung, Yoon Seok Choi, Su-Hyung Park, June-Won Cheong, Yoo Hong Min, Eui-Cheol Shin, Jin Seok Kim
Summary: By studying NK cells in patients with multiple myeloma, it was found that adaptive NK cells have lower levels of CD38 expression compared to conventional NK cells, and exhibit stronger anti-tumor activity in daratumumab-mediated effector functions. The composition of adaptive NK cells in the bone marrow of patients with multiple myeloma determines their ex vivo functional activity in response to daratumumab.
CLINICAL CANCER RESEARCH
(2021)
Article
Cell Biology
Masanobu Tsubaki, Tomoya Takeda, Yuuichi Koumoto, Takehiro Usami, Takuya Matsuda, Shiori Seki, Kazuko Sakai, Kazuto Nishio, Shozo Nishida
Summary: The development of BCR::ABL1 tyrosine kinase inhibitors has greatly improved survival in chronic myeloid leukaemia (CML) patients. However, resistance to these inhibitors is a clinical problem. This study investigated the mechanism of resistance to dasatinib, a BCR::ABL1 tyrosine kinase inhibitor, and found that elevated expression and activation of MOS, TPL2 and ERK1/2 were involved in dasatinib resistance. Inhibition of these proteins overcame the resistance.
CELL PROLIFERATION
(2023)
Review
Oncology
Claudio Cerchione, Franco Locatelli, Giovanni Martinelli
Summary: Acute leukemia, especially ALL, is the most common cancer in childhood with significantly improved survival rates over the years. The treatment for Ph+ALL in children has evolved from intensive chemotherapy and HSCT to a combination of TKIs and standard chemotherapy. The role of TKIs in pediatric Ph+ALL treatment has shown better outcomes compared to traditional treatments like HSCT.
FRONTIERS IN ONCOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Lucia Vrablova, Vladimir Divoky, Pavla Koralkova, Katerina Machova Polakova, Eva Kriegova, Romana Janska, Jan Grohmann, Milena Holzerova, Tomas Papajik, Edgar Faber
Summary: This article reports on two patients with lymphoid BC CML who were unable to undergo allogeneic stem cell transplantation. Instead, they received chemotherapy in combination with rituximab, imatinib, and dasatinib, achieving deep molecular response. These results suggest a re-evaluation of the use of combination drug therapy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Hematology
Nitin Jain, Abhishek Maiti, Farhad Ravandi, Marina Konopleva, Naval Daver, Tapan Kadia, Naveen Pemmaraju, Nicholas Short, Partow Kebriaei, Jing Ning, Jorge Cortes, Elias Jabbour, Hagop Kantarjian
Summary: The combination of bosutinib with inotuzumab ozogamicin showed promising efficacy and good tolerability in the treatment of relapsed/refractory Philadelphia chromosome positive acute lymphoblastic leukemia and lymphoid blast phase of chronic myeloid leukemia.
AMERICAN JOURNAL OF HEMATOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Min-Tsang Hsieh, Pei-Chih Lee, Yi-Ting Chiang, Hui-Yi Lin, Der-Yen Lee
Summary: This study aimed to investigate the effect of osimertinib and 35d on HCC827 cells and tumors by analyzing metabolome alterations and related regulations. The findings showed that both osimertinib and 35d disrupted fatty acid metabolism and induced mitochondrial stress, inhibiting cancer cell growth. These results provide insights into the potential application of osimertinib and 35d as combination therapy for tumors.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cardiac & Cardiovascular Systems
Wenying Jin, Sen Yang, Chao Yu, Tiangang Zhu, Qian Jiang
Summary: This study found that the use of dasatinib therapy is associated with pulmonary hypertension (PH) in patients with chronic myeloid leukemia (CML). Increasing age, pericardial effusion, cardiopulmonary comorbidities, and dasatinib as >= 3rd-line therapy were identified as risk factors for PH in these patients.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2022)
Article
Cardiac & Cardiovascular Systems
Jae-Sung Yi, Sravan Perla, Yan Huang, Kana Mizuno, Frank J. Giordano, Alexander A. Vinks, Anton M. Bennett
Summary: Low-dose dasatinib showed linear pharmacokinetic properties in NSML mice, with exposure-dependent inhibition of c-Src and PZR tyrosyl phosphorylation as well as AKT phosphorylation. A dose as low as 0.1 mg/kg of dasatinib prevented HCM in NSML mice and transcriptome analysis identified reversal of HCM-associated gene expression levels in the heart tissue.
CARDIOVASCULAR DRUGS AND THERAPY
(2022)
Article
Gastroenterology & Hepatology
Leonie C. S. De Vries, Mohammed Ghiboub, Patricia H. P. van Hamersveld, Olaf Welting, Caroline Verseijden, Matthew J. Bell, Inmaculada Rioja, Rabinder K. Prinjha, Pim J. Koelink, Birgit Strobl, Mathias Muller, Geert R. D. Haens, Manon E. Wildenberg, Wouter J. De Jonge
Summary: The study investigates the role of TYK2 and its inhibitor TYK2i in experimental colitis. TYK2i effectively alleviates symptoms of colitis in mice, while T cells with TYK2 kinase activity deficiency also exhibit similar effects. The differentiation of human Th1 cells is also affected by TYK2i.
JOURNAL OF CROHNS & COLITIS
(2021)
Review
Oncology
Fauziya, Akash Gupta, Arif Nadaf, Shadaan Ahmad, Nazeer Hasan, Mohammad Imran, Amirhossein Sahebkar, Gaurav Kumar Jain, Prashant J. Kesharwani, Farhan Ahmad
Summary: Dasatinib is a potent second generation TKI that shows promise in treating various forms of leukemia and cancer, being 300 times more potent than imatinib. With cancer being a leading cause of death worldwide, it is crucial to explore novel strategies to combat this disease. This review provides an evaluation of dasatinib, covering its therapeutic methodology, pharmacodynamics and pharmacokinetics, side effects, advantages, disadvantages, various interactions, and novel formulations.
Article
Mathematical & Computational Biology
Yuji Okamoto, Mitsuhito Hirano, Kai Morino, Masashi K. Kajita, Shinji Nakaoka, Mayuko Tsuda, Kei-ji Sugimoto, Shigehisa Tamaki, Junichi Hisatake, Hisayuki Yokoyama, Tadahiko Igarashi, Atsushi Shinagawa, Takeaki Sugawara, Satoru Hara, Kazuhisa Fujikawa, Seiichi Shimizu, Toshiaki Yujiri, Hisashi Wakita, Kaichi Nishiwaki, Arinobu Tojo, Kazuyuki Aihara
Summary: This study successfully classified CML patients with different nilotinib responses by using a dynamical model and laboratory findings. A method was proposed to identify patients who failed to achieve a treatment goal based on data collected at three initial time points only.
NPJ SYSTEMS BIOLOGY AND APPLICATIONS
(2022)
Article
Cell Biology
Valentina Cazzetta, Elena Bruni, Sara Terzoli, Claudia Carenza, Sara Franzese, Rocco Piazza, Paolo Marzano, Matteo Donadon, Guido Torzilli, Matteo Cimino, Matteo Simonelli, Lorenzo Bello, Anna Villa, Likai Tan, Sarina Ravens, Immo Prinz, Domenico Supino, Federico S. Colombo, Enrico Lugli, Emanuela Marcenaro, Eric Vivier, Silvia Della Bella, Joanna Mikulak, Domenico Mavilio
Summary: NKG2A expression identifies a subset of V delta 2 T cells with intrinsic hyper-responsiveness against cancer, which is counterbalanced by the inhibitory signaling delivered by HLA-E expressed on malignant cells. Removal of NKG2A restores the effector functions of V delta 2 T cells.
Article
Neurosciences
Eva M. J. Peters, Manfred Schedlowski, Carsten Watzl, Ulrike Gimsa
Summary: The COVID-19 pandemic has a significant impact on vulnerable populations and healthcare systems globally, with research suggesting that stress-related factors like socio-economic status may play a crucial role. Studies indicate that psychological stress may be linked to neuroendocrine-immune mechanisms, influencing SARS-CoV-2 infections and the progression of COVID-19 disease.
NEUROBIOLOGY OF STRESS
(2021)
Article
Rheumatology
Shuyang Zhao, Ricardo Grieshaber-Bouyer, Deepak A. Rao, Philipp Kolb, Haizhang Chen, Ivana Andreeva, Theresa Tretter, Hanns-Martin Lorenz, Carsten Watzl, Guido Wabnitz, Lars-Oliver Tykocinski, Wolfgang Merkt
Summary: Expanded HLA-DR+CD90+ synovial fibroblasts in patients with rheumatoid arthritis (RA) play a key role in inflammation. Activated NK cells induced the expression of HLA-DR on CD90+ synovial fibroblasts through IFNγ, leading to an inflammatory phenotype. JAK inhibition can target these proinflammatory synovial fibroblasts.
ARTHRITIS & RHEUMATOLOGY
(2022)
Article
Immunology
Doris Urlaub, Natalie Wolfsdorff, Deniz Durak, Frank Renken, Carsten Watzl
Summary: The study found that individuals who were infected as early as 10 days after their first vaccine dose had antibody levels comparable to fully vaccinated individuals.
IMMUNITY INFLAMMATION AND DISEASE
(2021)
Article
Health Care Sciences & Services
Patrick D. Gajewski, Stephan Getzmann, Peter Brode, Michael Burke, Cristina Cadenas, Silvia Capellino, Maren Claus, Erhan Genc, Klaus Golka, Jan G. Hengstler, Thomas Kleinsorge, Rosemarie Marchan, Michael A. Nitsche, Jorg Reinders, Christoph van Thriel, Carsten Watzl, Edmund Wascher
Summary: The Dortmund Vital Study aims to investigate the relationships between biological and environmental factors and cognitive functioning in healthy working adults. The study employs a combination of cross-sectional and longitudinal methods and collects a wide range of data, including demographics, psychology, behavior, cardiovascular health, and neuroimaging. The results of the study are expected to shed light on the individual differences in cognitive functioning with age and identify markers for healthy aging and cognitive decline.
JMIR RESEARCH PROTOCOLS
(2022)
Editorial Material
Immunology
Carsten Watzl
EUROPEAN JOURNAL OF IMMUNOLOGY
(2022)
Article
Immunology
Doris Urlaub, Natalie Wolfsdorff, Jan-Erik Hoffmann, Stefanie Dorok, Markus Hoffmann, Moritz Anft, Naomi Pieris, Patrick Guenther, Bernhard Schaaf, Uwe Cassens, Peter Broede, Maren Claus, Lea K. Picard, Sabine Wingert, Simone Backes, Deniz Durak, Nina Babel, Stefan Poehlmann, Frank Renken, Stefan Raunser, Carsten Watzl
Summary: Neutralizing antibodies against SARS-CoV-2 are found to be long-lasting and enhanced after vaccination in individuals who have recovered from COVID-19, providing clear benefits.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2022)
Article
Immunology
Lea Katharina Picard, Maren Claus, Frank Fasbender, Carsten Watzl
Summary: The engagement of CD56 can activate NK cells and induce degranulation, IFN-gamma secretion, and morphological changes, making CD56 a potential co-activating receptor in NK cells. This effect is dependent on cytokine stimulation and can be impaired by the inhibition of specific pathways. However, the absence of CD56 does not impact the cytotoxic activity of NK cells against tumor target cells.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2022)
Review
Cell Biology
Raghda A. Elsabbagh, Mona Rady, Carsten Watzl, Khaled Abou-Aisha, Mohamed Z. Gad
Summary: N6-methyl-adenosine (m(6)A) modification plays a crucial role in gene expression regulation and its dysregulation is associated with diseases. In the immune system, m(6)A modification regulates immune cell functions and immune responses.
CELL COMMUNICATION AND SIGNALING
(2022)
Article
Biology
Peter Broede, Maren Claus, Patrick D. Gajewski, Stephan Getzmann, Klaus Golka, Jan G. Hengstler, Edmund Wascher, Carsten Watzl
Summary: This study compared immunosenescence biomarkers with chronological age for predicting low cardiorespiratory fitness (CRF) and found that immune age could outperform chronological age as a predictor for low CRF. Factors such as obesity, physical inactivity, chronological age, and immune age were associated with increased probability of low CRF.
Article
Cell Biology
Lea Katharina Picard, Elisabeth Littwitz-Salomon, Herbert Waldmann, Carsten Watzl
Summary: Inhibition of glucose uptake can prevent NK cell proliferation and reduce stimulation-dependent IFN-gamma production, but does not affect cytotoxicity and serial killing activity.
Article
Chemistry, Analytical
Nour Sharar, Konstantin Wuestefeld, Rahat Morad Talukder, Julija Skolnik, Katharina Kaufmann, Bernd Giebel, Verena Boerger, Friedrich Nolte, Carsten Watzl, Frank Weichert, Roland Hergenroeder, Victoria Shpacovitch
Summary: This study introduces a wide-field surface plasmon resonance microscopy sensor that utilizes the surface plasmon resonance phenomenon to detect biological and non-biological nanoparticles. The sensor is able to detect and quantify biological nanoparticles without the need for labeling, using target-specific antibodies. The research also includes modifications to the optical sensor system and evaluates their effectiveness.
Article
Biochemistry & Molecular Biology
Peter Broede, Maren Claus, Patrick D. Gajewski, Stephan Getzmann, Edmund Wascher, Carsten Watzl
Summary: The definition of immunological aging type requires establishing reference intervals for immunosenescence biomarkers based on age distribution. We determined the comprehensive immune age index IMMAX for 1605 individuals and identified age-specific centiles, allowing categorization of individuals as immunologically slow or fast aging types. The IMMAX was rescaled to equivalent years of life (EYOL) using the 50th percentile as a reference, and the immunological age gap was calculated as the difference between EYOL and chronological age.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Letter
Urology & Nephrology
Arturo Blazquez-Navarro, Lisa Mittmann, Constantin Joachim Thieme, Moritz Anft, Krystallenia Paniskaki, Adrian Doevelaar, Felix Sebastian Seibert, Bodo Hoelzer, Margarete Justine Konik, Marc Moritz Berger, Thorsten Brenner, Clemens Tempfer, Carsten Watzl, Toni Luise Meister, Stephanie Pfaender, Eike Steinmann, Sebastian Dolff, Ulf Dittmer, Oliver Witzke, Ulrik Stervbo, Toralf Roch, Michal Or-Guil, Timm Henning Westhoff, Nina Babel
JOURNAL OF NEPHROLOGY
(2023)
Article
Immunology
Jana-Julia Siegler, Margareta P. Correia, Tomas Hofman, Isabel Prager, Emrullah Birgin, Nuh N. Rahbari, Carsten Watzl, Ana Stojanovic, Adelheid Cerwenka
Summary: ILC3s are cytokine-producing lymphocytes that directly respond to tumor cells, leading to enhanced IFN-gamma release and TRAIL-dependent cytotoxicity against cancer cells. This suggests that ILC3s may play a role in anti-tumor immunity and could be further utilized for cancer therapies.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Psychology, Clinical
Eva Milena Johanne Peters, Manfred Schedlowski, Carsten Watzl, Ulrike Gimsa
Summary: Excessive stress can compromise the barrier function of the airways and alter neuroendocrine control of immune function, creating a virus-permissive immune response. Identifying individuals with high psycho-social stress and assisting them in stress management is crucial, as certain forms of stress play a role in successful immune defense against viral respiratory diseases.
PSYCHOTHERAPIE PSYCHOSOMATIK MEDIZINISCHE PSYCHOLOGIE
(2021)