Article
Cell Biology
Miguel Reyes, Michael R. Filbin, Roby P. Bhattacharyya, Abraham Sonny, Arnav Mehta, Kianna Billman, Kyle R. Kays, Mayra Pinilla-Vera, Maura E. Benson, Lisa A. Cosimi, Deborah T. Hung, Bruce D. Levy, Alexandra-Chloe Villani, Moshe Sade-Feldman, Rebecca M. Baron, Marcia B. Goldberg, Paul C. Blainey, Nir Hacohen
Summary: The study found an expanded CD14(+) monocyte state, MS1, in patients with bacterial sepsis and severe COVID-19, with the gene expression program of MS1 correlating with sepsis severity and being up-regulated in monocytes from severe COVID-19 patients. In vitro induction of MS1 cells from healthy bone marrow HSPCs showed that plasma from sepsis or COVID-19 patients could induce myelopoiesis and expression of the MS1 gene program. The study also demonstrated that systemic cytokines such as IL-6 and IL-10 played a role in inducing myelopoiesis during severe bacterial or SARS-CoV-2 infection.
SCIENCE TRANSLATIONAL MEDICINE
(2021)
Article
Multidisciplinary Sciences
Shen Y. Y. Heazlewood, Tanveer Ahmad, Benjamin Cao, Huimin Cao, Melanie Domingues, Xuan Sun, Chad K. K. Heazlewood, Songhui Li, Brenda Williams, Madeline Fulton, Jacinta F. F. White, Tom Nebl, Christian M. M. Nefzger, Jose M. M. Polo, Benjamin T. T. Kile, Felix Kraus, Michael T. T. Ryan, Yu B. B. Sun, Peter F. M. Choong, Sarah L. L. Ellis, Minna-Liisa Anko, Susan K. K. Nilsson
Summary: Different sub-populations of megakaryocytes play important roles in generating platelets and regulating blood stem cells, and they are conserved in humans.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Tiago C. Luis, Nikolaos Barkas, Joana Carrelha, Alice Giustacchini, Stefania Mazzi, Ruggiero Norfo, Bishan Wu, Affaf Aliouat, Jose A. Guerrero, Alba Rodriguez-Meira, Tiphaine Bouriez-Jones, Iain C. Macaulay, Maria Jasztal, Guangheng Zhu, Heyu Ni, Matthew J. Robson, Randy D. Blakely, Adam J. Mead, Claus Nerlov, Cedric Ghevaert, Sten Eirik W. Jacobsen
Summary: In this study, the researchers uncover a feedback mechanism in which IL-1 secreted by activated platelets signals through niche Lepr+ cells to activate HSCs and restore platelet homeostasis.
NATURE COMMUNICATIONS
(2023)
Article
Medicine, Research & Experimental
Daphne N. Pariser, Zachary T. Hilt, Sara K. Ture, Sara K. Blick-Nitko, Mark R. Looney, Simon J. Cleary, Estheany Roman-Pagan, Jerry Saunders, Steve N. Georas, Janelle Veazey, Ferralita Madere, Laura Tesoro Santos, Allison Arne, Nguyen P. T. Huynh, Alison C. Livada, Selena M. Guerrero-Martin, Claire Lyons, Kelly A. Metcalf-Pate, Kathleen E. McGrath, James Palis, Craig N. Morrell
Summary: Platelets, previously known as cellular mediators of thrombosis, are also immune cells that interact with immune cells, impacting their activation and differentiation. Lung megakaryocytes (Mk(L)) have gene expression patterns similar to antigen-presenting cells, and have key immune regulatory roles influenced by the tissue environment. This indicates the plasticity of the immune phenotype of Mk(L) and their ability to induce T cell activation.
JOURNAL OF CLINICAL INVESTIGATION
(2021)
Article
Cell Biology
Burak H. Yalcin, Jadranka Macas, Eliza Wiercinska, Patrick N. Harter, Malak Fawaz, Tessa Schmachtel, Ilaria Ghiro, Ewa Bieniek, Djuro Kosanovic, Sonja Thom, Marcus Fruttiger, Makoto M. Taketo, Ralph T. Schermuly, Michael A. Rieger, Karl H. Plate, Halvard Bonig, Stefan Liebner
Summary: This study reveals the crucial role of β-catenin activation in the differentiation of megakaryocytes and red blood cells, promoting the development of megakaryocytes at the expense of erythropoiesis.
Review
Biochemistry & Molecular Biology
Joanna Lapczuk-Romanska, Maria Drozdzik, Stefan Oswald, Marek Drozdzik
Summary: The kidney is not only involved in metabolite elimination, but also plays a critical role in pharmacotherapy. Membrane carriers and transporters expressed in kidney tubule epithelial cells are important in drug elimination, determining drug nephrotoxicity and drug-drug interactions, as well as serving as direct drug targets. This review discusses these aspects of kidney transport proteins.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Oncology
Lilian Varricchio, Ronald Hoffman
Summary: Megakaryocytes play multiple roles in hematopoiesis, supporting HSPC development and providing inflammatory signals. Myelofibrosis, characterized by JAK/STAT hyperactivation and MK hyperplasia, is associated with abnormal inflammatory signatures. TGF beta 1 and S100A8/S100A9 complexes are crucial in the pathobiology of MF progression.
FRONTIERS IN ONCOLOGY
(2022)
Article
Hematology
Aled O'Neill, Desmond Chin, Darren Tan, A'Qilah Banu Bte Abdul Majeed, Ayako Nakamura-Ishizu, Toshio Suda
Summary: Thrombopoietin plays a crucial role in promoting megakaryocytic differentiation by maintaining hematopoietic cells with such potential, while also supporting hematopoietic stem and progenitor cell numbers. The loss of megakaryocytes due to thrombopoietin deficiency can be rescued by administration of recombinant CXCL4/PF4, which does not, however, restore reduced hematopoietic stem and progenitor cell numbers.
Review
Immunology
Yao Yao, Xiaoyu Cai, Fujia Ren, Yiqing Ye, Fengmei Wang, Caihong Zheng, Ying Qian, Meng Zhang
Summary: Osteoimmunity plays a crucial role in regulating the balance of bone remodeling and resorption, particularly through the macrophage-osteoclast axis. Understanding the role of this axis in osteoimmunity is significant for treating bone damage caused by inflammation and immune diseases.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Cell Biology
Bowen Zhang, Xumin Wu, Guicheng Zi, Lijuan He, Sihan Wang, Lin Chen, Zeng Fan, Xue Nan, Jiafei Xi, Wen Yue, Lei Wang, Liu Wang, Jie Hao, Xuetao Pei, Yanhua Li
Summary: By optimizing the culture protocol, a large number of megakaryocytes were successfully generated from human embryonic stem cells, confirming their biosafety and demonstrating their ability to form platelets. This study further enhances the clinical applications of megakaryocytes or platelets derived from pluripotent stem cells.
CELL PROLIFERATION
(2021)
Article
Multidisciplinary Sciences
Andreas Weigert, Xiang Zheng, Alina Nenzel, Kati Turkowski, Stefan Guenther, Elisabeth Strack, Evelyn Sirait-Fischer, Eiman Elwakeel, Ivan M. Kur, Vandana S. Nikam, Chanil Valasarajan, Hauke Winter, Alexander Wissgott, Robert Voswinkel, Friedrich Grimminger, Bernhard Brune, Werner Seeger, Soni Savai Pullamsetti, Rajkumar Savai
Summary: This study identifies the crucial role of fibrocytes in the progression and metastasis of lung cancer. Through experiments, it is demonstrated that fibrocytes can enhance the features of the cancer niche and promote cancer cell proliferation and migration. Lung cancer patients also exhibit an increased number of fibrocytes. The upregulation of endothelin and its receptors contributes to the interaction between fibrocytes and the cancer niche.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Javiera Villar, Lea Ouaknin, Adeline Cros, Elodie Segura
Summary: During inflammation, monocytes can differentiate into either macrophages or dendritic cells. This study used single-cell RNA sequencing and computational analysis to identify different pathways of differentiation for these two cell types. It also identified key transcription factors involved in monocyte fate commitment.
Article
Multidisciplinary Sciences
Jacqueline Feyen, Zhen Ping, Lanpeng Chen, Claire van Dijk, Tim V. D. van Tienhoven, Paulina M. H. van Strien, Remco M. Hoogenboezem, Michiel J. W. Wevers, Mathijs A. Sanders, Ivo P. Touw, Marc H. G. P. Raaijmakers
Summary: Myeloid cells reduce the function of HSCs through interferon signaling via a neutrophil-NK cell dependent process.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Birui Shi, Yan Zhang, Baolin Huang, Huiping Lin, Qiong Zhou, Yujue Wang, Zheng Cai, Menghua Liu
Summary: With the widespread clinical use of drug combinations, the incidence of drug-drug interactions (DDI) has significantly increased, leading to various adverse reactions. This study investigated the dynamic expression changes of drug transporters in tubulointerstitial fibrosis (TIF) kidneys and found that changes in serum creatinine can indicate changes in drug transporters in the kidney, affecting the pharmacokinetics of its substrates.
Article
Pharmacology & Pharmacy
Ruhul Kayesh, Vishakha Tambe, Chao Xu, Wei Yue
Summary: Impaired transport activity of hepatic OATP1B1 and OATP1B3 due to drug-drug interactions (DDIs) often leads to increased systemic exposure to substrate drugs. This study aimed to assess the OATP1B1- and OATP1B3-mediated DDI potential of the calcium channel blocker nicardipine. The results showed that nicardipine has the potential to cause OATP1B1/3-mediated DDIs, and the consideration of optimal preincubation conditions is important when assessing these interactions in vitro.
Article
Clinical Neurology
Ramanan Ganeshan, Manja Betz, Jan F. Scheitz, Hebun Erdur, Heinrich J. Audebert, Jochen B. Fiebach, Kersten Villringer
Summary: This study found that a significant proportion of patients with TGA exhibit acute ischemic cerebral lesions on MRI, highlighting the importance of performing MRI scans to identify possible cardiac involvement and detect stroke chameleons.
JOURNAL OF NEUROLOGY
(2022)
Article
Clinical Neurology
Simon Litmeier, Thomas R. Meinel, Regina von Rennenberg, Joachim U. Kniepert, Heinrich J. Audebert, Matthias Endres, Simon Jung, Jan F. Scheitz, Christian H. Nolte
Summary: This study analyzed the frequency of pathological findings in coronary angiography (CAG) in acute ischemic stroke patients and identified several clinical factors associated with the absence of obstructive coronary artery disease (CAD). The findings can assist in clinical decision-making for stroke patients with myocardial injury.
JOURNAL OF NEUROLOGY
(2022)
Article
Clinical Neurology
Filiz Osmanodja, Jan F. Scheitz, Jochen B. Fiebach, Ramanan Ganeshan, Kersten Villringer
Summary: The study found that intracranial time-of-flight (TOF) MRA can be a valuable diagnostic tool for predicting extracranial stenosis of the internal carotid artery (ICA).
JOURNAL OF NEUROLOGY
(2022)
Article
Geriatrics & Gerontology
Regina von Rennenberg, Thomas Liman, Christian H. Nolte, Alexander H. Nave, Jan F. Scheitz, Sandra Duezel, Vera Regitz-Zagrosek, Denis Gerstorf, Elisabeth Steinhagen-Thiessen, Ilja Demuth, Matthias Endres
Summary: There is evidence of an association between levels of high-sensitivity cardiac troponin T (hs-cTnT) and cognitive decline in older men, as measured by the Digit Symbol Substitution Test (DSST). This association remained significant even after adjusting for age, sex, education, and cardiovascular risk factors. However, there was no significant association between hs-cTnT and different cognitive domains at baseline.
Article
Clinical Neurology
Regina von Rennenberg, Juliane Herm, Thomas Krause, Simon Hellwig, Helena Stengl, Jan F. Scheitz, Thomas Elgeti, Sebastian N. Nagel, Matthias Endres, Karl Georg Haeusler, Christian H. Nolte
Summary: This study found that elevated cardiac biomarkers, such as high-sensitivity cardiac troponin T (hs-cTnT) and brain natriuretic peptide (BNP), in acute stroke patients are strongly associated with pathological findings on cardiac magnetic resonance imaging (CMR). The elevation of these biomarkers may help identify patients who require a more comprehensive cardiology work-up.
INTERNATIONAL JOURNAL OF STROKE
(2023)
Letter
Clinical Neurology
Tim Bastian Braemswig, Jan Vynckier, Marit Jensen, Florent Boutitie, Ivana Galinovic, Claus Z. Simonsen, Bastian Cheng, Tae-Hee Cho, Jan F. Scheitz, Jens Fiehler, Josep Puig, Vincent Thijs, Jochen B. Fiebach, Keith W. Muir, Norbert Nighoghossian, Martin Ebinger, Salvador Pedraza, Goetz Thomalla, Christian Gerloff, Matthias Endres, Robin Lemmens, Ludwig Schlemm, Christian H. Nolte
JOURNAL OF NEUROLOGY
(2022)
Article
Clinical Neurology
Alexandros A. Polymeris, Thomas R. Meinel, Hannah Oehler, Kyra Hoelscher, Annaelle Zietz, Jan F. Scheitz, Christian H. Nolte, Christoph Stretz, Shadi Yaghi, Svenja Stoll, Ruihao Wang, Karl Georg Haeusler, Simon Hellwig, Markus G. Klammer, Simon Litmeier, Christopher R. Leon Guerrero, Iman Moeini-Naghani, Patrik Michel, Davide Strambo, Alexander Salerno, Giovanni Bianco, Carlo Cereda, Timo Uphaus, Klaus Groeschel, Mira Katan, Susanne Wegener, Nils Peters, Stefan T. Engelter, Philippe A. Lyrer, Leo H. Bonati, Lorenz Grunder, Peter Arthur Ringleb, Urs Fischer, Bernd Kallmuenzer, Jan C. Purrucker, David J. Seiffge
Summary: This study investigated the causes, preventive strategies, and outcomes of stroke in atrial fibrillation patients despite anticoagulation. The results showed that stroke had diverse causes, and some patients experienced cardioembolism despite sufficient anticoagulation. Treatment with DOAC was associated with better outcomes compared to VKA, while adding antiplatelets was linked to worse outcomes in high-risk patients. These findings suggest the need for individualized and novel preventive strategies.
JOURNAL OF NEUROLOGY NEUROSURGERY AND PSYCHIATRY
(2022)
Article
Geriatrics & Gerontology
Wolfram Doehner, Nadja Scherbakov, Tim Schellenberg, Ewa A. Jankowska, Jan F. Scheitz, Stephan Haehling, Michael Joebges
Summary: This study found that iron deficiency and anemia are common and persistent findings in patients after acute stroke. Iron deficiency and anemia are independently related to lower functional capacity after acute stroke and to poor functional outcome after rehabilitation.
JOURNAL OF CACHEXIA SARCOPENIA AND MUSCLE
(2022)
Letter
Clinical Neurology
Christian H. Nolte, Jan F. Scheitz, Hebun Erdur, Heinrich J. Audebert
EUROPEAN STROKE JOURNAL
(2022)
Article
Clinical Neurology
Valerian L. Altersberger, Norman Rusche, Nicolas Martinez-Majander, Christian Hametner, Jan F. Scheitz, Hilde Henon, Maria Luisa Dell'Acqua, Davide Strambo, Jeffrey Stolp, Mirjam R. Heldner, Ilaria Grisendi, Dejana R. Jovanovic, Yannick Bejot, Alessandro Pezzini, Ronen R. Leker, Georg Kagi, Susanne Wegener, Carlo W. Cereda, Erik Lindgren, George Ntaios, Ines Piot, Alexandros A. Polymeris, Philippe A. Lyrer, Silja Raty, Gerli Sibolt, Marjaana Tiainen, Miriam Heyse, Hebun Erdur, Olfa Kaaouana, Visnja Padjen, Marialuisa Zedde, Marcel Arnold, Paul J. Nederkoorn, Patrik Michel, Guido Bigliardi, Andrea Zini, Charlotte Cordonnier, Christian H. Nolte, Peter A. Ringleb, Sami Curtze, Stefan T. Engelter, Henrik Gensicke
Summary: Based on data from a large registry, this study found that the safety of intravenous thrombolysis in very elderly patients with acute ischemic stroke is similar to that in younger patients. However, the probability of death and poor functional outcome was higher in the very elderly group.
Review
Cardiac & Cardiovascular Systems
Jan F. Scheitz, Luciano A. Sposato, Jeanette Schulz-Menger, Christian H. Nolte, Johannes Backs, Matthias Endres
Summary: Cardiovascular complications after ischemic stroke are significant and can lead to cardiac events. The stroke-heart syndrome provides a conceptual framework summarizing the neurocardiogenic mechanisms behind these events. However, differentiating between comorbid cardiovascular conditions and stroke-induced heart injury remains challenging at the individual patient level, requiring further research.
JOURNAL OF THE AMERICAN HEART ASSOCIATION
(2022)
Article
Clinical Neurology
Helena Stengl, Ramanan Ganeshan, Simon Hellwig, Markus G. Klammer, Regina von Rennenberg, Sophie Boehme, Heinrich J. Audebert, Christian H. Nolte, Matthias Endres, Jan F. Scheitz
Summary: Acute myocardial injury after acute ischemic stroke is associated with poor outcomes and is independently related to factors such as older age, higher stroke severity, thrombolytic treatment, and impaired kidney function.
EUROPEAN STROKE JOURNAL
(2022)
Article
Cardiac & Cardiovascular Systems
Tim Bastian Braemswig, Madeleine Kusserow, Barbara Bellmann, Frederik Beckhoff, Markus Reinthaler, Regina von Rennenberg, Hebun Erdur, Jan F. Scheitz, Ivana Galinovic, Kersten Villringer, David M. Leistner, Heinrich J. Audebert, Matthias Endres, Ulf Landmesser, Karl Georg Haeusler, Jochen B. Fiebach, Alexander Lauten, Andreas Rillig, Christian H. Nolte
Summary: New cerebral microbleeds (CMBs) occur in approximately one third of patients after catheter based structural heart interventions, and longer procedure duration may be a risk factor for new CMBs.
JOURNAL OF THE AMERICAN HEART ASSOCIATION
(2023)
Article
Clinical Neurology
Alexander Nelde, Markus G. Klammer, Christian H. Nolte, Helena Stengl, Michael Kraemer, Regina von Rennenberg, Andreas Meisel, Franziska Scheibe, Matthias Endres, Jan F. Scheitz, Christian Meisel
Summary: This study used data lake-enabled technology to assess post-stroke heart rate and heart rate variability, and found that HR modulation and including HR in machine learning models can improve stroke outcome prediction.
JOURNAL OF NEUROLOGY
(2023)
Article
Clinical Neurology
Simon Hellwig, Thomas Krause, Jan F. Scheitz, Juliane Herm, Ulrike Grittner, Nadja Jauert, Jochen B. Fiebach, Mario Kasner, Wolfram Doehner, Matthias Endres, Rolf Wachter, Thomas Elgeti, Christian H. Nolte, Karl Georg Haeusler
Summary: This study compared the rate of pathological findings in patients with acute ischemic stroke (AIS) between enhanced diagnostic workup and routine diagnostic care. The results showed that enhanced diagnostic workup yielded a higher rate of pathological findings and reduced the proportion of patients with cryptogenic stroke.
STROKE AND VASCULAR NEUROLOGY
(2023)