Review
Cell Biology
Qingyang Shi, Jiahuan Chen, Xiaodong Zou, Xiaochun Tang
Summary: Cholesterol homeostasis is important in understanding various diseases, and this review focuses on cholesterol synthesis, regulation, trafficking, and the role of lipid transfer proteins at organelle contact sites.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Richard Van Krieken, Yuan-Li Tsai, Anthony J. Carlos, Dat P. Ha, Amy S. Lee
Summary: Recent research has revealed that ER chaperones such as GRP78 can escape from the ER and traffic to the cell surface in colon and lung cancer via a non-traditional endosomal transport pathway mediated by Rab GTPases. This unconventional translocation mechanism is driven by membrane fusion between ER-derived vesicles and endosomes, requiring specific SNARE proteins and co-chaperones regulated by ER stress-induced signaling pathways.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Article
Microbiology
Anthony V. Morante, Daniel Davidnan Baboolal, Xavier Simon, Elizabeth, Mark Cheng-Ying Pan, Patricio I. Meneses
Summary: The amino acid residues 43-DQILQ-47 of the HPV16 L2 protein play a crucial role in the intracellular trafficking of the virus, particularly in trafficking beyond the endoplasmic reticulum.
MICROBIOLOGY SPECTRUM
(2022)
Review
Cell Biology
Eunhee Kim, Dong Min Lee, Min Ji Seo, Hong Jae Lee, Kyeong Sook Choi
Summary: Paraptosis is a type of programmed cell death characterized by dilation of the endoplasmic reticulum and/or mitochondria. Ca2+ transport has been shown to play an important role in paraptosis induced by various agents, and perturbations of cellular proteostasis and ion homeostasis are believed to contribute critically to the process. The communication between the ER and mitochondria mediated by Ca2+ appears to be crucial in inducing paraptosis.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Yaobin Liu, Rukmini Mukherjee, Florian Bonn, Thomas Colby, Ivan Matic, Marius Glogger, Mike Heilemann, Ivan Dikic
Summary: The SidE family of Legionella effectors, particularly SdeA, catalyzes non-canonical phosphoribosyl-linked ubiquitination of host proteins to disrupt Golgi integrity. This disruption prevents Golgi linking proteins GRASP55 and GRASP65 from clustering and forming oligomeric structures on multiple serine residues, impacting the host secretory pathway. The Golgi manipulation strategy by Legionella highlights how the bacterium hijacks the secretory pathway to promote bacterial infection.
CELL DEATH AND DIFFERENTIATION
(2021)
Review
Cell Biology
Kouminin Kanwore, Piniel Alphayo Kambey, Xiao-Xiao Guo, Ayanlaja Abdulrahman Abiola, Ying Xia, Dianshuai Gao
Summary: The external and internal factors of the cell play a critical role in glioma initiation, with multiple factors and molecules implicated in brain cancer progression. Glioma stem cells have been identified as the origin, and oncogene overexpression is involved in tumorigenesis, maintenance, and progression. Additionally, defects in cell organelles contribute to cancer cell resistance to drugs, apoptosis, and immune cells.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Pharmacology & Pharmacy
Carmine Pasquale Cerrato, Ulo Langel
Summary: This review provides an overview of CPP technologies for intracellular organelle targeting, discussing their pharmacological and therapeutical applications. Targeted drug delivery to specific organelles offers improved efficacy, control of mode of action, and potential to overcome drug resistance mechanisms.
EXPERT OPINION ON DRUG DELIVERY
(2022)
Review
Biochemistry & Molecular Biology
Na Song, Ming Yang, Hao Zhang, Shi-kun Yang
Summary: Kidney disease is a serious health concern, and understanding the pathogenesis is crucial for precise therapies. Disruption of calcium homeostasis plays a fundamental role in kidney disease development and is a promising therapeutic strategy. The endoplasmic reticulum and mitochondria are key organelles, with pharmacologic modulation of cellular calcium homeostasis as a novel treatment approach.
CURRENT MEDICINAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Leena Haataja, Anoop Arunagiri, Anis Hassan, Kaitlin Regan, Billy Tsai, Balamurugan Dhayalan, Michael A. Weiss, Ming Liu, Peter Arvan
Summary: Efficient export of proinsulin from the endoplasmic reticulum requires the formation of specific disulfide bonds, while disrupting certain pairings may improve trafficking in some mutants. Mutations causing Mutant INS gene-induced Diabetes of Youth (MIDY) often result in perturbed proinsulin disulfide bond formation, with potential for allele-specific pharmacological targeting in the future.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Article
Chemistry, Multidisciplinary
Elisabeth Andersen, Maria Eugenia Chollet, Francesco Bernardi, Alessio Branchini, Marcello Baroni, Guglielmo Mariani, Alberto Dolce, Angelika Batorova, Ellen Skarpen, Christiane Filion Myklebust, Grethe Skretting, Per Morten Sandset
Summary: Patients with the FVII variant p.A354V-p.P464Hfs display large variations in clinical phenotypes, possibly influenced by genetic and acquired factors. The chemical chaperone 4-PBA can improve the intracellular trafficking of the variant, leading to increased secretion, but has minimal impact on biological activity.
APPLIED SCIENCES-BASEL
(2021)
Article
Multidisciplinary Sciences
Rakshya Gorkhali, Li Tian, Bin Dong, Pritha Bagchi, Xiaonan Deng, Shrikant Pawar, Duc Duong, Ning Fang, Nicholas Seyfried, Jenny Yang
Summary: This study revealed the first CaSR protein-protein interactome and demonstrated the role of Ca-ex(2+) in promoting interactions related to Ca2+ dynamics and endoplasmic reticulum-related processes. The findings suggest that CaSR is involved in regulating Ca-ER(2+) dynamics, agonist-mediated ER-protein processing, and surface expression.
SCIENTIFIC REPORTS
(2021)
Review
Biochemistry & Molecular Biology
Selma Yilmaz Dejgaard, John F. Presley
Summary: This review focuses on the role of membrane trafficking in the life cycle of lipid droplets, including their biogenesis, attachment, catabolism, and eventual demise, with particular attention to the involvement of small GTPases such as Rab18 in these processes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Anastasia D. Teplova, Artemii A. Pigidanov, Marina V. Serebryakova, Sergei A. Golyshev, Raisa A. Galiullina, Nina V. Chichkova, Andrey B. Vartapetian
Summary: Soluble chaperones in the ER are crucial for protein folding and quality control, while certain ER resident proteins in plants possess an ER retrieval signal that can be cleaved by a specific protease.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Editorial Material
Cell Biology
Jeffrey Knupp, Yu-Jie Chen, Anoop Arunagiri, Leena Haataja, Peter Arvan, Billy Tsai
Summary: The study reveals that RTN3 recruits misfolded prohormones for lysosomal degradation through PGRMC1, selectively targeting small oligomers rather than large protein aggregates. This finding suggests that PGRMC1 could be a potential intervention point for diseases caused by ER protein retention.
Article
Plant Sciences
Fangfang Niu, Changyang Ji, Zizhen Liang, Rongfang Guo, Yixuan Chen, Yonglun Zeng, Liwen Jiang
Summary: ADP-ribosylation factor (ARF) family proteins play a central role in regulating vesicular traffic and organelle structures in eukaryotes. This study characterized the function of a unique ARF protein, ARFD1B, in Arabidopsis. It was found that ARFD1B is required for cell plate formation, maintenance of Golgi morphology, and plant growth in Arabidopsis by interacting with COAT PROTEIN COMPLEX I components.
Article
Engineering, Biomedical
Yongdeok Kim, Gelson Pagan-Diaz, Lauren Gapinske, Yerim Kim, Judy Suh, Emilia Solomon, Jennifer Foster Harris, SungWoo Nam, Rashid Bashir
ADVANCED HEALTHCARE MATERIALS
(2020)
Article
Multidisciplinary Sciences
Yu Zhang, Hui Li, Yi-Li Min, Efrain Sanchez-Ortiz, Jian Huang, Alex A. Mireault, John M. Shelton, Jiwoong Kim, Pradeep P. A. Mammen, Rhonda Bassel-Duby, Eric N. Olson
Article
Cell Biology
Miao Cui, Zhaoning Wang, Kenian Chen, Akansha M. Shah, Wei Tan, Lauren Duan, Efrain Sanchez-Ortiz, Hui Li, Lin Xu, Ning Liu, Rhonda Bassel-Duby, Eric N. Olson
DEVELOPMENTAL CELL
(2020)
Article
Biotechnology & Applied Microbiology
Yi-Li Min, Francesco Chemello, Hui Li, Cristina Rodriguez-Caycedo, Efrain Sanchez-Ortiz, Alex A. Mireault, John R. McAnally, John M. Shelton, Yu Zhang, Rhonda Bassel-Duby, Eric N. Olson
Article
Cell & Tissue Engineering
Emilia A. Solomon, Allison M. Rooney, Arasely M. Rodriguez, Sofiya Micheva-Viteva, Rashid Bashir, Rashi Iyer, Jennifer Foster Harris
Summary: The study describes a reliable and simple analytical system using genetically modified cells and optimized culture medium to measure the effect of chemicals on the connection between motor neurons and skeletal muscles. Nondestructive neuronal stimulation and easy recording of electrical response have a broad range of applications including faster long-term screening of drugs affecting the NMJ.
TISSUE ENGINEERING PART C-METHODS
(2021)
Article
Cell Biology
Emilia Solomon, Katie Davis-Anderson, Blake Hovde, Sofiya Micheva-Viteva, Jennifer Foster Harris, Scott Twary, Rashi Iyer
Summary: The study identified and characterized gene networks triggered by chemical reprogramming iPSC into motor neurons, providing insights into the differentiation process. The findings have implications for novel therapeutic approaches in regenerative medicine.
BMC MOLECULAR AND CELL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
F. Chemello, A. C. Chai, H. Li, C. Rodriguez-Caycedo, E. Sanchez-Ortiz, A. Atmanli, A. A. Mireault, N. Liu, R. Bassel-Duby, E. N. Olson
Summary: This study demonstrated the use of adenine base editor to restore dystrophin expression in cardiomyocytes affected by Duchenne muscular dystrophy mutation, as well as reframing the dystrophin open reading frame. In vivo experiments with mice showed successful gene editing and disease correction, indicating the potential of nucleotide editing for diverse DMD mutations with minimal genome modification.
Article
Medicine, Research & Experimental
Yu Zhang, Takahiko Nishiyama, Hui Li, Jian Huang, Ayhan Atmanli, Efrain Sanchez-Ortiz, Zhaoning Wang, Alex A. Mireault, Pradeep P. A. Mammen, Rhonda Bassel-Duby, Eric N. Olson
Summary: A study introduced an efficient gene-editing method using SaCas9 to correct the commonly affected exon 51 in DMD, which showed promising therapeutic effects in correcting DMD in a mouse model through a single AAV9 delivery system.
MOLECULAR THERAPY-METHODS & CLINICAL DEVELOPMENT
(2021)
Article
Cell & Tissue Engineering
Julie B. Strickland, Katie Davis-Anderson, Sofiya Micheva-Viteva, Scott Twary, Rashi Iyer, Jennifer Foster Harris, Emilia A. Solomon
Summary: Neuromuscular junction (NMJ) models are important tools for studying NMJ development, disease modeling, and high-throughput screening. Stem cell differentiation and patient-derived induced pluripotent stem cells provide valuable insights. Advanced bioengineering techniques have propelled the field forward.
TISSUE ENGINEERING PART B-REVIEWS
(2022)
Article
Medicine, Research & Experimental
Yu Zhang, Hui Li, Takahiko Nishiyama, John R. McAnally, Efrain Sanchez-Ortiz, Jian Huang, Pradeep P. A. Mammen, Rhonda Bassel-Duby, Eric N. Olson
Summary: In this study, a humanized DMD mouse model was created to evaluate the efficacy of CRISPR-Cas9 gene editing for DMD correction. The model successfully restored dystrophin expression and improved pathological features and muscle strength. This model provides a valuable tool for assessing clinically relevant gene editing strategies and advancing the therapeutic translation of CRISPR-Cas9 gene editing for DMD correction.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2022)
Article
Multidisciplinary Sciences
Simon Lebek, Francesco Chemello, Xurde M. Caravia, Wei Tan, Hui Li, Kenian Chen, Lin Xu, Ning Liu, Rhonda Bassel-Duby, Eric N. Olson
Summary: CRISPR-Cas9 gene editing is a promising therapy for genomic mutations, but current approaches are limited to specific mutations in a small group of patients. This study introduces a cardioprotective strategy that can potentially benefit a broader range of heart disease patients. By using base editing to eliminate oxidation-sensitive methionine residues in the CaMKII6 gene, the researchers demonstrated protection against ischemia/reperfusion injury in human cardiomyocytes and restored heart function in mice.
Article
Cell Biology
Takahiko Nishiyama, Yu Zhang, Miao Cui, Hui Li, Efrain Sanchez-Ortiz, John R. McAnally, Wei Tan, Jiwoong Kim, Kenian Chen, Lin Xu, Rhonda Bassel-Duby, Eric N. Olson
Summary: Mutations in the RBM20 gene are a common cause of familial dilated cardiomyopathy. The study uses gene editing techniques to correct these mutations and demonstrates that correction can restore normal gene splicing and nuclear localization, while eliminating abnormal ribonucleoprotein granule formation. The effectiveness of this therapy is also validated in a mouse model.
SCIENCE TRANSLATIONAL MEDICINE
(2022)
Retraction
Medicine, Research & Experimental
Huijuan Shi, Hui Li, Tiantian Zhen, Yu Dong, Xiaojuan Pei, Xiangliang Zhang
MOLECULAR THERAPY-NUCLEIC ACIDS
(2022)
Article
Biochemistry & Molecular Biology
Tao Long, Yichi Zhang, Linda Donnelly, Hui Li, Yu-Chung Pien, Ning Liu, Eric N. Olson, Xiaochun Li
Summary: The researchers determined the structure of the key protein Myomaker involved in myoblast fusion and found that it adopts a GPCR-like fold. They also identified the dimeric interface and lipid-binding sites as crucial for fusion and proposed a mechanism that may be conserved between different species.
NATURE STRUCTURAL & MOLECULAR BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Francesco Chemello, Zhaoning Wang, Hui Li, John R. McAnally, Ning Liu, Rhonda Bassel-Duby, Eric N. Olson
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Oncology
Yuxin Liu, Swati Bhardwaj, Keith Sigel, John Winters, Joseph Terlizzi, Michael M. Gaisa
Summary: This study investigated the prevalence and severity of anal HPV disease among MSM LWH under the age of 35, finding a high prevalence of HPV infection and precancer but no cases of invasive anal cancer. This supports the adoption of age-based anal cancer screening for this population.
INTERNATIONAL JOURNAL OF CANCER
(2024)
Correction
Oncology
J. Gu, S. Xie, S. Wang
INTERNATIONAL JOURNAL OF CANCER
(2024)