Article
Multidisciplinary Sciences
Emilie Giraud, Chloe Otero del Val, Celia Caillet-Saguy, Nada Zehrouni, Cecile Khou, Joel Caillet, Yves Jacob, Nathalie Pardigon, Nicolas Wolff
Summary: The study revealed that the PBM of NS5 from West Nile virus interacts with PDZ-containing proteins in human cells, playing a crucial role in viral replication. Knockdown of specific PDZ-containing proteins resulted in decreased WNV replication, highlighting the significance of these interactions in the virus life cycle.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Boglarka Zambo, Gergo Gogl, Bastien Morlet, Pascal Eberling, Luc Negroni, Herve Moine, Gilles Trave
Summary: This study comprehensively investigates the PDZ-PBM interactome of diacylglycerol kinases (DGKs) using interactomic approaches and evolutionary analysis, revealing functional differences between PBMs of different types of DGKs.
Article
Biochemistry & Molecular Biology
Mariano Martin, Lisa Salleron, Victoria Peyret, Romina Celeste Geysels, Elisabeth Darrouzet, Sabine Lindenthal, Carlos Eduardo Bernal Barquero, Ana Maria Masini-Repiso, Thierry Pourcher, Juan Pablo Nicola
Summary: NIS gene mutations leading to impaired iodide accumulation cause congenital hypothyroidism, as demonstrated by a truncated NIS protein missing some amino acids mislocalized into intracellular vesicles. Further investigation reveals the involvement of the PDZ domain in the interaction between the protein SCRIB and NIS, affecting the plasma membrane localization of NIS.
Article
Microbiology
Yanlei Zhu, Flavio Alvarez, Nicolas Wolff, Ariel Mechaly, Sebastien Brule, Benoit Neitthoffer, Sandrine Etienne-Manneville, Ahmed Haouz, Batiste Boeda, Celia Caillet-Saguy
Summary: The C-terminus of the SARS-CoV-2 protein E contains a PDZ-binding motif (PBM) that interacts with human PDZ-containing proteins involved in cellular junctions and polarity. Crystal structures of complexes between human LNX2, MLLT4, and MPP5 PDZs and SARS-CoV-2 E PBM were solved, revealing the binding preferences of the PBM for cellular targets. Mutations in the E protein near the PBM may have important effects on its structure and ion-channel activity, as well as the host machinery targeted by the variants during infection.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Mariano Genera, Baptiste Colcombet-Cazenave, Anastasia Croitoru, Bertrand Raynal, Ariel Mechaly, Joel Caillet, Ahmed Haouz, Nicolas Wolff, Celia Caillet-Saguy
Summary: The protein tyrosine phosphatase non-receptor type 3 (PTPN3) has a PDZ domain that plays both tumor-suppressive and tumor-promoting roles in cancer. This study focuses on the interactions between the PDZ domain of PTPN3 and the PDZ-binding motifs (PBMs) of viral and cellular protein partners. The study identifies key structural determinants of PBM recognition by PTPN3 and reveals the inhibitory role of the PDZ domain on the protein's phosphatase activity.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Wareerat Umnajvijit, Jariya Sangthong, Fabien Loison, Kulthida Vaeteewoottacharn, Mathurose Ponglikitmongkol
Summary: This study identifies the internal class III PBM of HPV16E6*I as a key element required for Dlg degradation.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2021)
Article
Multidisciplinary Sciences
Sachiko Fujiwara, Thanh Phuong Nguyen, Kyoko Furuse, Yugo Fukazawa, Tetsuhisa Otani, Mikio Furuse
Summary: The interaction of claudins with ZO proteins is not a prerequisite for tight junction formation at the most apical part of cell-cell contacts.
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES
(2022)
Article
Urology & Nephrology
Monica Ceol, Lisa Gianesello, Hernan Trimarchi, Alberto Migliorini, Giovanna Priante, Claudia M. Radu, Elena Naso, Annalisa Angelini, Lorenzo A. Calo, Franca Anglani, Dorella Del Prete
Summary: This study investigated the role of ClC-5, megalin, and cubilin in parietal epithelial cells and found that the number of hypertrophic parietal epithelial cells was significantly higher in lupus nephritis patients. The presence of these cells in Bowman's capsule may provide a potential resource for responding to protein overload in other glomerulonephritis.
JOURNAL OF NEPHROLOGY
(2023)
Article
Biochemistry & Molecular Biology
Celia Caillet-Saguy, Fabien Durbesson, Veronica V. Rezelj, Gergo Gogl, Quang Dinh Tran, Jean-Claude Twizere, Marco Vignuzzi, Renaud Vincentelli, Nicolas Wolff
Summary: Small linear motifs targeting the PDZ domains have been identified at the C terminus of SARS-CoV-2 proteins E, 3a, and N. By profiling the human PDZome, potential human protein targets of SARS-CoV-2 have been identified, some of which may play roles in viral replication and immune responses.
Article
Multidisciplinary Sciences
Jason J. Kwon, Behnoush Hajian, Yuemin Bian, Lucy C. Young, Alvaro J. Amor, James R. Fuller, Cara V. Fraley, Abbey M. Sykes, Jonathan So, Joshua Pan, Laura Baker, Sun Joo Lee, Douglas B. Wheeler, David L. Mayhew, Nicole S. Persky, Xiaoping Yang, David E. Root, Anthony M. Barsotti, Andrew W. Stamford, Charles K. Perry, Alex Burgin, Frank McCormick, Christopher T. Lemke, William C. Hahn, Andrew J. Aguirre
Summary: This study uses cryo-electron microscopy to determine the structure of the SHOC2-MRAS-PP1C complex and investigate the functional consequences of SHOC2 missense variants. The research reveals the mechanism of complex formation and the enhanced activity of mutant versions. This study is of great importance for understanding cellular signal transduction and the pathogenesis of RAS-related diseases.
Article
Pharmacology & Pharmacy
Amany A. Azouz, Dina A. Hanna, Ali A. Abo-Saif, Basim Anwar Shehata Messiha
Summary: This study investigated the potential effects of montelukast on megalin expression/endocytic function against gentamicin nephrotoxicity. The results suggest that montelukast can interfere with megalin expression/endocytic function, reducing renal cell apoptosis and improving kidney function.
SAUDI PHARMACEUTICAL JOURNAL
(2022)
Article
Cell Biology
Michelle America, Naguissa Bostaille, Marie Eubelen, Maud Martin, Didier Y. R. Stainier, Benoit Vanhollebeke
Summary: This study reveals the molecular mechanisms underlying the interactions between Gpr124 and Frizzled in zebrafish and mammals, and provides insights into the evolution of Gpr124/Reck function in vertebrates.
Article
Physiology
Jianchun Chen, Xiaoyong Wang, Qian He, Raymond C. Harris
Summary: Podocyte is a crucial component of the glomerular filtration barrier, and the role of Yes-associated protein (YAP)/transcriptional coactivator with PDZ-binding motif (TAZ) in podocytes has been investigated. The study found that TAZ is constitutively active in podocytes and plays a critical role in the expression of synaptopodin, zonula occludens-1, and zonula occludens-2, which are important for podocyte function and structure.
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Dhruv C. Rathod, Sonali M. Vaidya, Marie-T. Hopp, Toni Kuehl, Diana Imhof
Summary: Heme plays a dual role in biological processes, functioning as a prosthetic group of hemoproteins and also regulating biochemical pathways through transient association with proteins. However, the mechanisms of heme recognition and complex formation with target proteins are poorly understood. This report focuses on evaluating mammalian heme-regulated proteins and their heme-binding motifs (HBMs), particularly the Cys-Pro dipeptide motifs. This analysis provides insights into the sequence and structural anomalies observed during transient heme binding and protein regulation.
Article
Biochemistry & Molecular Biology
Jihyun Lee, Yujin Jung, Seo won Jeong, Ga Hee Jeong, Gue Tae Moon, Miri Kim
Summary: The Hippo signaling pathway and its effectors, YAP and TAZ, are found to play critical roles in angiogenesis and have potential therapeutic implications for rosacea. In a mouse model, inhibiting YAP and TAZ improved clinical features of rosacea and reduced VEGF immunoreactivity, suggesting their potential as therapeutic targets.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Nutrition & Dietetics
Nirajan Shrestha, Simone Sleep, Tessa Helman, Olivia Holland, James S. M. Cuffe, Anthony Perkins, Andrew J. McAinch, John P. Headrick, Deanne H. Hryciw
Summary: Maternal high linoleic acid diets have significant impacts on offspring's circulating lipids, fatty acids, and cardiac function, particularly affecting later dietary modifications in female offspring.
BRITISH JOURNAL OF NUTRITION
(2022)
Article
Biochemistry & Molecular Biology
Phillip Gough, Oliver Bown, Craig R. Campbell, Philip Poronnik, Pauline M. Ross
Summary: Biomedical science students need to learn coding for future research and workforce needs. However, incorporating coding in the curriculum poses challenges, which can be overcome through a creative coding approach. The study found diverse student responses, with a majority supporting creative coding and a minority opposing it. The use of Processing and Arduino platforms to visualize physiological data enriched the learning experience.
BIOCHEMISTRY AND MOLECULAR BIOLOGY EDUCATION
(2023)
Article
Education, Scientific Disciplines
Sarah J. Etherington, Andrew J. Moorhouse, Tamara M. Paravicini, Michelle Towstoless, Alan Hayes, Deanne H. Hryciw, Louise Lexis, Kathy Tangalakis, Task Task Force
Summary: A task force of physiology educators from 25 Australian universities reached a nationwide consensus on seven core concepts for physiology curricula, one of which is cell membrane. The cell membrane core concept was unpacked into four themes and 33 subthemes, covering the definition, structure, transport, and potentials of cell membranes. Most items were evaluated as essential or important, with cell membrane structure theme rated as relatively less important and membrane potential theme rated as the most difficult. The importance of cell membranes in biomedical education received strong support from Australian educators.
ADVANCES IN PHYSIOLOGY EDUCATION
(2023)
Article
Biochemistry & Molecular Biology
Nirajan Shrestha, Nathan Rout-Pitt, Alexandra McCarron, Courtney A. A. Jackson, Andrew C. C. Bulmer, Andrew J. J. McAinch, Martin Donnelley, David W. W. Parsons, Deanne H. H. Hryciw
Summary: This study aimed to investigate the characteristics of essential fatty acid deficiency caused by cystic fibrosis (CF), which is the result of mutations in the CF transmembrane conductance regulator (CFTR). Two rodent models of CF were studied: one strain with a loss of phenylalanine at position 508 (Phe508del) in CFTR and the other lacking functional CFTR (510X). Fatty acid concentrations were measured in the serum, and the expression of genes related to fatty acid transport and metabolism was quantified. Histological assessment of the ileal tissue was also performed. The results showed genotype- and age-dependent alterations in fatty acid concentration, as well as changes in gene expression and tissue morphology. These findings suggest that CF rat models exhibit abnormalities in the handling of fatty acids, possibly due to altered transport and metabolism, as well as fibrosis and structural changes in the ileum.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Lannie O'Keefe, Teresa Vu, Anna C. C. Simcocks, Kayte A. A. Jenkin, Michael L. L. Mathai, Andrew J. J. McAinch, Dana S. S. Hutchinson, Deanne H. H. Hryciw
Summary: Diet-induced obesity (DIO) leads to alterations in hormones, lipids, and inflammation, with the cannabinoid type 2 receptor (CB2) contributing to the inflammatory response. The effects of modulating CB2 with pharmacological treatments in a DIO model were investigated. The treatments reduced adipose tissue mRNA levels related to thermogenesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Editorial Material
Neurosciences
Deanne H. Hryciw
JOURNAL OF PHYSIOLOGY-LONDON
(2023)
Article
Education, Scientific Disciplines
Christian Moro, Tracy Douglas, Ruben Phillips, Michelle Towstoless, Alan Hayes, Deanne H. Hryciw, Louise Lexis, Kathy Tangalakis, Task Force
Summary: Using the Delphi method, consensus was reached on seven core concepts of physiology, including the integration concept of cells, tissues, organs, and organ systems interacting to create and sustain life. A team of Australian physiology educators unpacked this core concept into hierarchical levels, identifying five themes and ten subthemes. The importance and difficulty levels of each theme and subtheme were rated by experienced physiology educators. The main theme of hierarchy was rated as essential and slightly difficult, while the other subthemes differed in importance and difficulty. Integration core concepts should be taught in the later semesters of a Physiology program.
ADVANCES IN PHYSIOLOGY EDUCATION
(2023)
Article
Education, Scientific Disciplines
Daniel Brown, Tanya Uebergang, Nicole Masters, Michelle Towstoless, Alan Hayes, Deanne H. Hryciw, Louise Lexis, Kathy Tangalakis
Summary: This article aims to unpack the core concept of substance movement within the human body, providing guidance for physiology teaching in tertiary education institutes in Australia. The concept introduces fundamental knowledge of the driving factors and applies them in physiological contexts. The importance of understanding this concept is widely acknowledged, but its difficulty varies among educators. The complexity of this concept is due to the underlying physical forces, and organizing it into subthemes can help prioritize learning activities.
ADVANCES IN PHYSIOLOGY EDUCATION
(2023)
Article
Education, Scientific Disciplines
Elizabeth A. H. Beckett, Voula Gaganis, Anthony J. Bakker, Michelle Towstoless, Alan Hayes, Deanne H. Hryciw, Louise Lexis, Kathy Tangalakis, Task Force
Summary: Australia-wide consensus was reached on seven core concepts of physiology, with homeostasis being one of the fundamental concepts for students to understand physiological regulatory mechanisms. A team of Australian educators unpacked the concept and identified 5 themes and 18 subthemes to simplify the components and improve learning and teaching of homeostasis.
ADVANCES IN PHYSIOLOGY EDUCATION
(2023)
Article
Education, Scientific Disciplines
Kathy Tangalakis, Louise Lexis, Deanne H. Hryciw, Michelle Towstoless, Anthony J. Bakker, Elizabeth Beckett, Daniel Brown, Melissa Cameron, Julia Choate, Lisa Chopin, Matthew B. Cooke, Tracy Douglas, Suzanne Estaphan, Sarah Etherington, Voula Gaganis, Andrew Moorhouse, Christian Moro, Tamara Paravicini, Ben Perry, Ruben Phillips, Christopher Scott, Gabrielle Todd, Tanya Uebergang, Glenn Wadley, Matthew Watt, Alan Hayes
Summary: Consensus has been reached on the core concepts of physiology in Australia through the Delphi method, and these concepts will be integrated into the curricula of Australian universities. The core concepts include Cell Membrane, Cell-Cell Communication, Movement of Substances, Structure and Function, Homeostasis, Integration, and Physiological Adaptation.
ADVANCES IN PHYSIOLOGY EDUCATION
(2023)
Article
Education, Scientific Disciplines
Ben D. Perry, Melissa S. Cameron, Matthew B. Cooke, Michelle Towstoless, Deanne H. Hryciw, Alan Hayes, Louise Lexis, Kathy Tangalakis, Task Force
Summary: The structure and function core concept of physiology was unpacked and validated by Australian educators, specifically focusing on the renal system.
ADVANCES IN PHYSIOLOGY EDUCATION
(2023)
Article
Education, Scientific Disciplines
Lisa K. Chopin, Julia Choate, Joseph A. Rathner, Michelle Towstoless, Alan Hayes, Deanne H. Hryciw, Louise Lexis, Kathy Tangalakis, Task Force
Summary: An Australia-wide consensus was reached on seven core concepts of physiology, one of which was cell-cell communication. Three physiology educators from a core concepts Delphi task force unpacked this core concept into seven different themes and 60 subthemes. Cell-cell communication, previously unpacked and validated, was modified for an Australian audience to include emerging knowledge and adapted to increase student accessibility.
ADVANCES IN PHYSIOLOGY EDUCATION
(2023)
Article
Education, Scientific Disciplines
Kathy Tangalakis, Brianna L. Julien, Louise Lexis, Deanne H. Hryciw, Colleen J. Thomas, Maja Husaric, Michelle Towstoless, Philip J. MacKinnon, Yuan Miao, Alan Hayes
Summary: This study aimed to investigate the extent to which a set of 15 core concepts of physiology are reflected in the learning outcomes of physiology curricula in Australian universities. The findings suggest a lack of alignment of learning outcomes with the core concepts and highlight the need for agreement on a set of core concepts in physiology in Australia.
ADVANCES IN PHYSIOLOGY EDUCATION
(2023)
Article
Biochemistry & Molecular Biology
Kun He, Mengyi Xie, Weifeng Hong, Yonghe Li, Yaolin Yin, Xiaojin Gao, Yi He, Yu Chen, Chuan You, Jingdong Li
Summary: Centromere protein L (CENPL) is overexpressed in hepatocellular carcinoma (HCC) and is associated with poor patient prognosis. Upregulation of CENPL promotes tumor proliferation and glycolysis in HCC cells by activating the MEK1/2-ERK1/2 signaling pathway.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Yingzi Wang, Haozhong Huang, Huimin Weng, Chunsen Jia, Bin Liao, Yang Long, Fengxu Yu, Yongmei Nie
Summary: Talin protein plays a crucial role in transmitting mechanical forces by connecting the extracellular matrix to the cytoskeleton. It converts mechanical signals into biochemical signals and serves as diagnostic, therapeutic, and prognostic indicators in diseases.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2024)
Review
Biochemistry & Molecular Biology
Hema Saranya Ilamathi, Marc Germain
Summary: Mitochondria are the central metabolic hubs in cells, relying on proteins encoded by nuclear DNA and mitochondrial DNA (mtDNA) for their function. The maintenance and distribution of mtDNA are crucial for proper mitochondrial function and are associated with mitochondrial diseases. mtDNA is organized into nucleoids that dynamically move and interact with each other. The replication and distribution of mtDNA nucleoids are regulated by the complex interplay of mitochondrial dynamics, ER-mitochondria contact sites, and cytoskeletal networks.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
K. K. Sruthi, Sirisha Natani, Ramesh Ummanni
Summary: The overexpression of TPD52 is associated with the emergence of neuroendocrine prostate cancer (NEPC). TPD52 activates the NF-kappa B - STAT3 axis to induce neuroendocrine differentiation (NED) of prostate cancer cells. Therapeutic targeting of TPD52 is important for the treatment of prostate cancer.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Cui Li, Feifan Xiang, Yuqi Gong, Yi Fu, Ge Chen, Zhi Wang, Zhong Li, Daiqing Wei
Summary: Our study demonstrates the significant role of tumor-derived microparticles (T-MPs) in osteosarcoma metastasis and immune response. T-MPs promote macrophage polarization and stimulate migration and chemoresistance of osteosarcoma cells, thereby affecting the progression of osteosarcoma.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2024)