Article
Food Science & Technology
Ana Paula Aparecida Pereira, Celio Fernando Figueiredo Angolini, Jane Cristina de Souza-Sporkens, Tomaz Antonio da Silva, Helena Coutinho Franco de Oliveira, Glaucia Maria Pastore
Summary: The study revealed that the extract from ju-açu fruits, rich in bioactive compounds, could protect mitochondrial functionality and stimulate insulin secretion, making it a potential adjuvant therapy for diabetes.
FOOD RESEARCH INTERNATIONAL
(2021)
Article
Chemistry, Multidisciplinary
Svetlana Ponomareva, Helene Joisten, Taina Francois, Cecile Naud, Robert Morel, Yanxia Hou, Thomas Myers, Isabelle Joumard, Bernard Dieny, Marie Carriere
Summary: This study presents a new approach to induce insulin release from beta-islet pancreatic cells by mechanical stimulation. The researchers used magnetic particles and magnetic fields to stimulate the cells, and measured insulin release to evaluate the cell response. The results demonstrated that mechanical action is an efficient stimulus for insulin secretion, potentially allowing for the design of a system that triggers insulin secretion on demand.
Article
Environmental Sciences
Hai-Ming Xu, Meng-Yu Wu, Xin-Chen Shi, Ke-Liang Liu, Ying-Chi Zhang, Yin-Feng Zhang, Hong-Mei Li
Summary: This study investigated the impact of PFOS exposure on GSIS in INS-1 cells and the potential protective effects of PC. After 48 h of exposure, the effects of PFOS and/or PC on GSIS were examined. Results showed that PFOS exposure did not significantly affect cell viability but reduced insulin secretion and relative mRNA expression levels. Furthermore, PFOS exposure led to an increase in ROS activity, which was alleviated by PC intervention.
Article
Pharmacology & Pharmacy
Carine Marmentini, Dimitrius Santiago P. S. F. Guimaraes, Tanes de Lima, Francisco Breno S. Teofilo, Natalia S. da Silva, Gabriela M. Soares, Antonio C. Boschero, Mirian A. Kurauti
Summary: The study demonstrates that rosiglitazone can improve the viability of INS-1E cells exposed to hIAPP, independent of insulin-degrading enzyme (IDE) modulation. Rosiglitazone inhibited hIAPP fibrillation and reduced hIAPP-induced expression of CHOP, ATF4, and p-eIF2a, suggesting that TZDs treatment may be a promising approach to preserve β-cell mass and function.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2022)
Article
Plant Sciences
Dahae Lee, Ki Hyun Kim, Taesu Jang, Ki Sung Kang
Summary: The phytochemical examination of Cornus walteri led to the isolation of a tirucallane triterpenoid, (-)-leucophyllone, which was found to increase glucose-stimulated insulin secretion in rat INS-1 pancreatic beta-cells. Treatment with (-)-leucophyllone also resulted in increased expression of proteins related to insulin signaling, suggesting its potential as an antidiabetic agent.
Article
Plant Sciences
Dahae Lee, Ji-Young Kim, Hak Cheol Kwon, Jaeyoung Kwon, Dae Sik Jang, Ki Sung Kang
Summary: In this study, the potential effects of alpha-Spinasterol, a phytosterol isolated from Aster pseudoglehnii, on glucose uptake and insulin secretion in skeletal muscle cells and pancreatic beta-cells were investigated. The results showed that alpha-Spinasterol enhanced glucose uptake and insulin secretion without causing any toxic effects on the cells. It also increased the expression of proteins related to glucose metabolism. These findings suggest that alpha-Spinasterol could be a potential candidate for the treatment of type 2 diabetes.
Article
Chemistry, Medicinal
Dahae Lee, Jaekyung Kim, Sungyoul Choi, Jinwon Choi, Jin Woo Lee, Ki Sung Kang, Sang Hee Shim
Summary: Pancreatic beta-cell function and insulin secretion are important in antidiabetic drug development. In this study, an endophytic fungus was investigated for its potential in regulating insulin secretion. Ten compounds were isolated and found to increase glucose-stimulated insulin secretion in rat pancreatic beta-cells. Further investigation revealed the involvement of a signaling pathway in the activation of PPAR gamma and PDX-1.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2023)
Article
Multidisciplinary Sciences
Lianbin Xu, Xueyan Lin, Xiuli Li, Zhiyong Hu, Qiuling Hou, Yun Wang, Zhonghua Wang
Summary: The application of mass spectrometry allows for the detection of metabolic differences between organisms with different nutritional conditions. This study systematically captured the divergence in metabolic fingerprints of rat pancreatic INS-1 beta-cells in relation to the availability of ten individual essential amino acids (EAAs), providing valuable insights into the metabolic processes affected by diverse EAAs.
Article
Biochemistry & Molecular Biology
Dahae Lee, Young-Mi Kim, Hyun Woo Kim, You-Kyoung Choi, Bang Ju Park, Sang Hoon Joo, Ki Sung Kang
Summary: Schisandrol A and schisandrin C enhanced insulin secretion and glucose uptake, with schisandrin C showing superior effects compared to gliclazide. Treatment with schisandrin C resulted in increased expression of associated proteins in cells, potentially benefiting in improving hyperglycemia.
Article
Endocrinology & Metabolism
Minami Kosuda, Midori Yamana, Akiko Nagasawa, Aya Tanaka, Kentaro Watanabe, Hisamitsu Ishihara
Summary: This study revealed that mitochondrial CA VB and cytosolic CA XIII are significantly increased in highly glucose-responsive insulin-secreting cells, while cytosolic CA XI is decreased. Overexpression of CA VB increases insulin secretion, while overexpression of CA XIII and CA XI has no effect on insulin secretion.
JOURNAL OF BIOLOGICAL REGULATORS AND HOMEOSTATIC AGENTS
(2023)
Article
Environmental Sciences
Xiaoyu Duan, Weijie Sun, Hongwen Sun, Lianying Zhang
Summary: The study revealed that PFASs adversely affect insulin secretion, with exposure to PFOS inhibiting SIRT1 activity and leading to impairment of GSIS.
ENVIRONMENTAL POLLUTION
(2021)
Article
Materials Science, Biomaterials
Fan Yang, Jiajia Wang, Kaige Qu, Huimiao Wei, Zhengxun Song, Hongmei Xu, Zuobin Wang, Yujuan Chen
Summary: Diabetes has been a global public health problem for many years, leading to an exploration of the complex mechanism of insulin secretion in pancreatic β cells. Recent advancements in nanoscale resolution characterization allowed insights into the mechanical and functional processes at the single cell level. Glucose stimulation experiments using atomic force microscopy revealed the interplay between physical interactions and the biochemical process of INS-1 cells. Changes in material properties and insulin secretion of INS-1 cells in response to glucose patterns provided valuable knowledge on the regulation of glucose and the potential application in therapy.
BIOMATERIALS ADVANCES
(2023)
Article
Endocrinology & Metabolism
Yan Yang, Zixin Cai, Zhenhong Pan, Fen Liu, Dandan Li, Yujiao Ji, Jiaxin Zhong, Hairong Luo, Shanbiao Hu, Lei Song, Shaojie Yu, Ting Li, Jiequn Li, Xianhua Ma, Weiping Zhang, Zhiguang Zhou, Feng Liu, Jingjing Zhang
Summary: The small GTPase Rheb1 plays a critical role in regulating glucose-stimulated insulin secretion in beta-cells, suggesting a potential new target for therapeutic treatment of diabetes.
METABOLISM-CLINICAL AND EXPERIMENTAL
(2021)
Article
Pharmacology & Pharmacy
Suhadinie Gamage, Mirabela Hali, Anjaneyulu Kowluru
Summary: CARD9 plays a crucial role in regulating insulin secretion, potentially through a Rac1-independent and p38-dependent signaling module.
BIOCHEMICAL PHARMACOLOGY
(2021)
Article
Pharmacology & Pharmacy
Huizhen Sun, Anyong Zhang, Yanchun Gong, Wei Sun, Baiyi Yan, Shuihong Lei, Li-Hua Yao
Summary: Cordycepin promotes insulin synthesis and secretion, possibly through increased expression of insulin-related genes and proteins, elevated ATP levels, and higher Ca2+ concentrations. In addition, Cordycepin protects pancreatic beta cells from oxidative damage, improving their viability and insulin synthesis/secretion.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Petr Jezek, Blanka Holendova, Martin Jaburek, Andrea Dlaskova, Lydie Plecita-Hlavata
Summary: Mitochondria play a crucial role in glucose-stimulated insulin secretion (GSIS) in pancreatic beta-cells by increasing ATP synthesis. Recent studies have shown that mitochondrial redox signaling and Ca2+ influx are important for insulin secretion, while future research aims to further clarify these mechanisms.
ANTIOXIDANTS & REDOX SIGNALING
(2022)
Article
Biochemistry & Molecular Biology
Pavla Pruchova, Klara Gotvaldova, Katarina Smolkova, Lukas Alan, Blanka Holendova, Jan Tauber, Alexander Galkin, Petr Jezek, Martin Jaburek
Summary: This study reveals the antioxidant and anti-inflammatory roles of iPLA(2)gamma in the brain. It demonstrates that iPLA(2)gamma is activated by tert-butylhydroperoxide and releases free fatty acids, which leads to an increase in respiratory rate and a decrease in mitochondrial superoxide production. It also shows that iPLA(2)gamma plays a key role in the remodeling of cardiolipins in the brain.
Meeting Abstract
Biochemistry & Molecular Biology
Petr Jezek, Blanka Holendova, Lydie Plecita-Hlavata, Pavla Pruchova, Hana Engstova, Martin Jaburek
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2022)
Article
Biochemistry & Molecular Biology
Min Li, Lydie Plecita-Hlavata, Evgenia Dobrinskikh, B. Alexandre McKeon, Aneta Gandjeva, Suzette Riddle, Aya Laux, Ram Raj Prasad, Sushil Kumar, Rubin M. Tuder, Hui Zhang, Cheng-Jun Hu, Kurt R. Stenmark
Summary: The study found that abnormal SIRT3 activity is related to mitochondrial dysfunction in adventitial fibroblasts from patients with pulmonary hypertension. Restoration of SIRT3 activity can reduce mitochondrial protein acetylation and restore mitochondrial function.
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Petr Jezek, Martin Jaburek, Blanka Holendova, Hana Engstova, Andrea Dlaskova
Summary: The mitochondrial reticulum network in cells consists of parallel lamellar cristae formed by invaginated inner mitochondrial membrane. The non-invaginated part, the inner boundary membrane, forms a cylindrical sandwich with the outer mitochondrial membrane. The dimensions, shape, and junctions of cristae have characteristic patterns for different metabolic regimes, physiological and pathological situations.
ANTIOXIDANTS & REDOX SIGNALING
(2023)
Article
Biochemistry & Molecular Biology
Ippei Kawano, Bazila Bazila, Petr Jezek, Andrea Dlaskova
Summary: The architecture of the mitochondrial network and cristae critically impact cell differentiation and identity. Recent studies have shown that manipulating mitochondrial dynamics and cristae shape affects T cell phenotype, macrophage polarization, and other cellular processes. Further investigation of the molecular mechanisms involved could lead to improved therapeutic manipulation of cell viability, differentiation, proliferation, and identity.
ANTIOXIDANTS & REDOX SIGNALING
(2023)
Article
Multidisciplinary Sciences
Vojtech Pavluch, Tomas Spacek, Hana Engstova, Andrea Dlaskova, Petr Jezek
Summary: Using 3D-double-color direct stochastic optical reconstruction microscopy (dSTORM) and a new method of Delaunay segmentation of 3D data, we identified the presence of two or more mitochondrial DNA molecules in nucleoids in HeLa cells, indicating a low level of nucleoid division. This finding is important for understanding the organization and distribution of mitochondrial DNA.
SCIENTIFIC REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Romana Bohuslavova, Valeria Fabriciova, Laura Lebron-Mora, Jessica Malfatti, Ondrej Smolik, Lukas Valihrach, Sarka Benesova, Daniel Zucha, Zuzana Berkova, Frantisek Saudek, Sylvia M. Evans, Gabriela Pavlinkova
Summary: By using transgenic mouse models, transcriptomic and epigenomic profiling, we found that the absence of Isl1 leads to a diabetic phenotype, loss of alpha cells, disrupted pancreatic islet architecture, downregulation of key ss-cell regulators and maturation markers, and an enrichment in an intermediate endocrine progenitor transcriptomic profile.
CELL AND BIOSCIENCE
(2023)
Article
Immunology
Lydie Plecita-Hlavata, Andrea Brazdova, Monika Krivonoskova, Cheng-Jun Hu, Tzu Phang, Jan Tauber, Min Li, Hui Zhang, Konrad Hoetzenecker, Slaven Crnkovic, Grazyna Kwapiszewska, Kurt R. Stenmark
Summary: In pulmonary hypertension (PH), perivascular inflammation accompanied by pulmonary arterial remodeling is observed. This study aimed to investigate the contribution of pulmonary fibroblasts to the activation and polarization of T-cells in PH.
FRONTIERS IN IMMUNOLOGY
(2023)
Review
Endocrinology & Metabolism
Daniel Benak, Stepanka Benakova, Lydie Plecita-Hlavata, Marketa Hlavackova
Summary: The emerging field of epitranscriptomics has attracted attention due to its significant effects on gene expression and cellular physiology and pathophysiology. N-6-methyladenosine (m(6)A) and N-6,2'-O-dimethyladenosine (m(6)Am) are well-studied modifications in RNA that are dynamically regulated by epitranscriptomic regulators. Changes in m(6)A levels and regulatory proteins have been associated with diabetic tissues. This review provides an overview of the current knowledge on m(6)A and m(6)Am modifications and their regulators in the pathophysiology of diabetes mellitus, with a focus on type 2 diabetes and its treatment with metformin.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Petr Jezek
Summary: This article theoretically derives the mutual influence between mitochondria and the cytosol, predicting possible conditions for mitochondrial signals to the cytosol and plasma membrane. The involvement of peroxiredoxin 3 in mitochondrial-to-cytosol redox signaling is discussed, as well as the activation of matrix MnSOD in a specific case. The use of redox-sensitive probes and their impact on quantifying H2O2 fluxes is also considered.
Article
Engineering, Biomedical
Zuzana Berkova, Klara Zacharovova, Alzbeta Patikova, Ivan Leontovyc, Zuzana Hladikova, David Cerveny, Eva Tihlarikova, Vilem Nedela, Peter Girman, Daniel Jirak, Frantisek Saudek
Summary: This study aimed to develop an extracellular matrix skeleton from the native pancreas that could support islet survival in the omentum. The perfusion through the splenic vein was found to be the most effective decellularization approach. The decellularized pancreatic skeleton could support long-term islet survival in the omentum.
JOURNAL OF FUNCTIONAL BIOMATERIALS
(2022)
Meeting Abstract
Surgery
B. Hagerf, Z. Hladikova, M. Zahradnicka, K. Kesslerova, T. Sosna, P. Girman, F. Saudek
AMERICAN JOURNAL OF TRANSPLANTATION
(2022)
Meeting Abstract
Endocrinology & Metabolism
B. Hagerf (Voglova), Z. Hladikova, L. Nemetova, M. Zahradnicka, K. Kesslerova, T. Sosna, F. Saudek
Article
Biochemistry & Molecular Biology
Kun He, Mengyi Xie, Weifeng Hong, Yonghe Li, Yaolin Yin, Xiaojin Gao, Yi He, Yu Chen, Chuan You, Jingdong Li
Summary: Centromere protein L (CENPL) is overexpressed in hepatocellular carcinoma (HCC) and is associated with poor patient prognosis. Upregulation of CENPL promotes tumor proliferation and glycolysis in HCC cells by activating the MEK1/2-ERK1/2 signaling pathway.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Yingzi Wang, Haozhong Huang, Huimin Weng, Chunsen Jia, Bin Liao, Yang Long, Fengxu Yu, Yongmei Nie
Summary: Talin protein plays a crucial role in transmitting mechanical forces by connecting the extracellular matrix to the cytoskeleton. It converts mechanical signals into biochemical signals and serves as diagnostic, therapeutic, and prognostic indicators in diseases.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2024)
Review
Biochemistry & Molecular Biology
Hema Saranya Ilamathi, Marc Germain
Summary: Mitochondria are the central metabolic hubs in cells, relying on proteins encoded by nuclear DNA and mitochondrial DNA (mtDNA) for their function. The maintenance and distribution of mtDNA are crucial for proper mitochondrial function and are associated with mitochondrial diseases. mtDNA is organized into nucleoids that dynamically move and interact with each other. The replication and distribution of mtDNA nucleoids are regulated by the complex interplay of mitochondrial dynamics, ER-mitochondria contact sites, and cytoskeletal networks.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
K. K. Sruthi, Sirisha Natani, Ramesh Ummanni
Summary: The overexpression of TPD52 is associated with the emergence of neuroendocrine prostate cancer (NEPC). TPD52 activates the NF-kappa B - STAT3 axis to induce neuroendocrine differentiation (NED) of prostate cancer cells. Therapeutic targeting of TPD52 is important for the treatment of prostate cancer.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Cui Li, Feifan Xiang, Yuqi Gong, Yi Fu, Ge Chen, Zhi Wang, Zhong Li, Daiqing Wei
Summary: Our study demonstrates the significant role of tumor-derived microparticles (T-MPs) in osteosarcoma metastasis and immune response. T-MPs promote macrophage polarization and stimulate migration and chemoresistance of osteosarcoma cells, thereby affecting the progression of osteosarcoma.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2024)