Journal
INTERNATIONAL IMMUNOPHARMACOLOGY
Volume 14, Issue 4, Pages 463-470Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.intimp.2012.09.006
Keywords
APS; Adjuvant; HBV; DNA vaccine; Dendritic cell; Regulatory T cell
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Funding
- Two Sides Supporting Plan in Sichuan Agriculture University [00770103]
- Changing Scholars and Innovative Research Team in University [IRT0848]
- Sichuan Education Commission [09ZA072]
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Astragalus polysaccharides (APS), an extract from a kind of Chinese traditional herb Astragalus membranaceus, was proved to have strong immunoregulatoiy properties. In this study, APS was employed as an adjuvant of Hepatitis B virus (HBV) DNA vaccine (pcDS2) and its' effects on immune system of mice were investigated. Our data demonstrated that APS as an adjuvant could increase the HBsAg-specific antibody level as well as the proliferating activity of T cells. APS also could induce CD4(+) T cells to produce IL-4, IL-2 and IFN-gamma and enhance IFN-gamma expression of CD8(+) T cells. Moreover, APS could induce the robust activity of the cytotoxic lymphocytes (CTL). Additionally, APS could stimulate the dendritic cells (DC) maturation which is characterized by up-regulation of MHC I/II, CD40, CD80 and CD86, and decreased the frequency of the regulatory T cells (nTreg). Collectively, these findings suggest that APS is a potent adjuvant for the hepatitis B DNA vaccine and can enhance the immune responses of HBV DNA vaccine via promoting DC maturation and inhibit the Treg frequency. (C) 2012 Elsevier B.V. All rights reserved.
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