Article
Ecology
Huan Yang, Chong Juan You, Clement K. M. Tsui, Luke R. Tembrock, Zhi Qiang Wu, De Po Yang
Summary: The study of 53 specimens of Japanese rhinoceros beetle from 44 locations in five Asian countries using specific-locus amplified fragment sequencing (SLAF-seq) techniques revealed three distinct genetic groups and the importance of geographic factors in population divergence. High level of differentiation among populations/groups was demonstrated, as well as measurable gene flow between genetic clusters at varying rates and directions.
ECOLOGY AND EVOLUTION
(2021)
Article
Engineering, Chemical
Chika Takai-Yamashita, Seiji Yamashita, Yuya Mabuchi, Atsushi Teramae, Takuya Matsuyama, Yuki Taguchi, Taiga Mushika, Yuya Wada, Shinta Fitria Novasari, Junko Ikeda, Yutaka Ohya
Summary: The Mahalanobis-Taguchi system (MTS) was used to classify droppings from Japanese rhinoceros beetle larva into male/female based on their shape-related features. The best variable combination for complete classification was found to be projected area/Feret diameter and solidity. These variables were affected by gut components, as determined by time-domain nuclear magnetic resonance (TD-NMR).
ADVANCED POWDER TECHNOLOGY
(2022)
Article
Ecology
Jillian F. del Sol, Yoshihito Hongo, Romain P. Boisseau, Gabriella H. Berman, Cerisse E. Allen, Douglas. J. Emlen
Summary: Research on rhinoceros beetles suggests that sexual selection on horn length may be influenced by local habitat conditions and the relative abundance of feeding territories, leading to differences in male weapon size selection among different populations.
Article
Multidisciplinary Sciences
Haruhiko Adachi, Makoto Ozawa, Satoshi Yagi, Makoto Seita, Shigeru Kondo
Summary: The last larval instars of Trypoxylus dichotomus burrow in two different ways depending on the hardness of the soil, which is thought to be an adaptation to diverse soil conditions. These results are crucial for understanding soil ecology and pose a challenge for engineers developing newer excavation technology.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Shinichi Morita, Tomoko F. Shibata, Tomoaki Nishiyama, Yuuki Kobayashi, Katsushi Yamaguchi, Kouhei Toga, Takahiro Ohde, Hiroki Gotoh, Takaaki Kojima, Jesse N. Weber, Marco Salvemini, Takahiro Bino, Mutsuki Mase, Moe Nakata, Tomoko Mori, Shogo Mori, Richard Cornette, Kazuki Sakura, Laura C. Lavine, Douglas J. Emlen, Teruyuki Niimi, Shuji Shigenobu
Summary: In this study, the genome and transcriptome of the Japanese rhinoceros beetle were sequenced and analyzed, providing insights into the formation of beetle horns and the evolutionary mechanisms of sexually dimorphic genes.
SCIENTIFIC REPORTS
(2023)
Article
Biology
Qingyun Wang, Liwei Liu, Sujiong Zhang, Hong Wu, Junhao Huang
Summary: This study presents the well-assembled and annotated genome of the xylophagous beetle Trypoxylus dichotomus, providing insights into the functional and evolutionary mechanisms of lignocellulose digestion. The analysis revealed rapidly evolving gene families and digestive genes associated with the high degradation efficiency of lignocellulose in T. dichotomus. Comparative transcriptome analysis showed differential expression patterns in digestive-related genes among different gut segments. Chromosome breakage and rearrangement events were also observed during the evolution of T. dichotomus.
Article
Biology
Shinichi Morita, Kazuki Sakura, Hiroki Gotoh, Douglas J. Emlen, Teruyuki Niimi
Summary: The exaggerated horns of beetles are ideal models for studying the origin of novel traits and morphological evolution. Different closely related species show significant differences in the size, number, shape, and body region of their horns. Beetle horns also exhibit nutrition-dependent phenotypic plasticity, with the largest and best-condition individuals showing disproportionate growth of their horns, while poor-condition individuals have much smaller or even stunted horns. Recent research using genome sequencing has revealed these exciting phenomena in beetle horns.
CURRENT OPINION IN INSECT SCIENCE
(2022)
Article
Virology
Eunsun Kim, Ji-Young Kim, Wontae Kim, Seokhyun Lee, Kwan-Ho Park, June-Sun Yoon
Summary: This study provides a detailed analysis of the genomic characteristics of a Nudivirus infection in Korean rhinoceros beetles. The research reveals the high conservation of the virus in terms of genomic structure and size compared to other related viruses. Additionally, significant mutations were observed in the core genes of the virus. These findings serve as a valuable resource for understanding cross-species Nudivirus transmission.
Article
Biochemistry & Molecular Biology
Mitsutoshi Tabata, Kazuo Umetsu, Takashi Kitano
Summary: The Japanese rhinoceros beetle produces lectins allo A-I and allo A-II with strong N-acetyllactosamine-binding activity, formed from three subunits (alpha, beta, and gamma). The cDNA sequences of these subunits revealed a gene family with high sequence identities among them. Unknown proteins with the DUF3421 domain were found in a protein database search, indicating a potential role for allo A genes in larvae.
BIOCHEMICAL GENETICS
(2022)
Article
Multidisciplinary Sciences
Sugihiko Hoshizaki
Summary: This study investigated the developmental progression of individual larval head size in Trypoxylus dichotomus, finding that the growth trajectory of individual head size is correlated with pre-molt head size and developmental stage. Additionally, the relationship between head size and body size was examined, showing covariation between the two. However, few previous studies have compared the ontogenetic progression of individual head sizes with that of individual body sizes.
Article
Entomology
Daniel M. Jenkins, Shizu Watanabe, Ron P. Haff, Michael J. Melzer, Eric Jackson, Pei-Shih Liang
Summary: The study found that eggs and larvae of the Coconut Rhinoceros Beetle are highly susceptible to X-ray irradiation, while larger doses are needed to render adults incapable of reproducing. Sterilized adults may survive for more than a month at exposures near 50 Gy, but X-ray irradiation would likely not be an effective tool for field control due to rapid attenuation of radiation energy in the organic nesting materials tested.
JOURNAL OF APPLIED ENTOMOLOGY
(2021)
Article
Biology
Kazuki Sakura, Shinichi Morita, Teruyuki Niimi
Summary: In this study, candidate genes associated with a specific phenotype in the Japanese rhinoceros beetle Trypoxylus dichotomus were identified using next-generation sequencing analysis. The functions of these genes were investigated using the RNA interference (RNAi) method, which is the only gene function analysis tool currently available for T. dichotomus. A simplified procedure for the RNAi method, requiring only one syringe, was developed for injecting double-stranded RNA (dsRNA) into T. dichotomus.
Article
Agronomy
Sarah Mansfield, Balanama Asigau, Chikako van Koten, Sulav Paudel, Mike Bowie, Trevor A. Jackson, Sean D. G. Marshall
Summary: This study examines damage assessment of coconut palms caused by coconut rhinoceros beetle (CRB) from the perspectives of participants, power, and purpose. The results indicate that participants were more accurate using a binary damage scale and that more complex scales did not increase the likelihood of detecting significant changes in damage. Recommendations for standardizing damage assessment were also developed.
NEW ZEALAND JOURNAL OF CROP AND HORTICULTURAL SCIENCE
(2023)
Article
Entomology
M. Sujithra, M. Rajkumar, Priyank Hanuman Mhatre, Govindharaj Guru-Pirasanna-Pandi
Summary: Based on virulence, reproductive capacity, searching ability, and LC50 values, strain Sc1 has the potential to be a biocontrol agent for managing Oryctes in coconut palms in humid tropical ecosystems. Further field efficacy testing of this native strain against Oryctes rhinoceros is recommended to include EPNs in the integrated pest management program in coconuts.
EGYPTIAN JOURNAL OF BIOLOGICAL PEST CONTROL
(2022)
Article
Entomology
Meng-Wei Shen, Hung-Chuan Chen, Shyi-Tien Chen
Summary: The Oryctes rhinoceros beetle, a native and widely spread species in Southeast Asia, has been shown to efficiently degrade certain persistent organic pollutants (POPs). Through an acclimation process, the tolerance of beetle larvae to these pollutants can be increased, reducing mortality rates. This alternative method shows promise for environmental remediation and warrants further investigation.
Article
Zoology
Maria R. Mills, Rahmi S. Nemri, Emily A. Carlson, William Wilde, Hiroki Gotoh, Laura C. Lavine, Brook O. Swanson
JOURNAL OF EXPERIMENTAL ZOOLOGY PART A-ECOLOGICAL GENETICS AND PHYSIOLOGY
(2016)
Article
Biotechnology & Applied Microbiology
Hiroki Gotoh, Robert A. Zinna, Ian Warren, Michael DeNieu, Teruyuki Niimi, Ian Dworkin, Douglas J. Emlen, Toru Miura, Laura C. Lavine
Article
Biochemistry & Molecular Biology
Xinda Lin, Yun Yao, Bo Wang, Mark D. Lavine, Laura Corley Lavine
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2016)
Article
Biochemistry & Molecular Biology
Xinda Lin, Yili Xu, Yun Yao, Bo Wang, Mark D. Lavine, Laura Corley Lavine
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2016)
Article
Zoology
R. Zinna, H. Gotoh, C. S. Brent, A. Dolezal, A. Kraus, T. Niimi, D. Emlen, L. C. Lavine
INTEGRATIVE AND COMPARATIVE BIOLOGY
(2016)
Article
Biochemistry & Molecular Biology
Mariany Ashanty Morales, Bianca Marie Mendoza, Laura Corley Lavine, Mark Daniel Lavine, Douglas Bruce Walsh, Fang Zhu
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2016)
Article
Biochemistry & Molecular Biology
Xinda Lin, Yun Yao, Bo Wang, Douglas J. Emlen, Laura Corley Lavine
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2016)
Review
Entomology
Fang Zhu, Laura Lavine, Sally O'Neal, Mark Lavine, Carrie Foss, Douglas Walsh
Article
Entomology
James Hust, Mark D. Lavine, Amy M. Worthington, Robert Zinna, Hiroki Gotoh, T. Niimi, Laura Lavine
JOURNAL OF INSECT PHYSIOLOGY
(2018)
Article
Multidisciplinary Sciences
Xinda Lin, Yili Xu, Jianru Jiang, Mark Lavine, Laura Corley Lavine
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2018)
Review
Biology
Xinda Lin, Laura Corley Lavine
CURRENT OPINION IN INSECT SCIENCE
(2018)
Article
Entomology
Meixiang Wu, Adekunle W. Adesanya, Mariany A. Morales, Douglas B. Walsh, Laura C. Lavine, Mark D. Lavine, Fang Zhu
JOURNAL OF PEST SCIENCE
(2019)
Article
Genetics & Heredity
Takahiro Ohde, Shinichi Morita, Shuji Shigenobu, Junko Morita, Takeshi Mizutani, Hiroki Gotoh, Robert A. Zinna, Moe Nakata, Yuta Ito, Kenshi Wada, Yasuhiro Kitano, Karen Yuzaki, Kouhei Toga, Mutsuki Mase, Koji Kadota, Jema Rushe, Laura Corley Lavine, Douglas J. Emlen, Teruyuki Niimi
Article
Entomology
A. W. Adesanya, M. A. Morales, D. B. Walsh, L. C. Lavine, M. D. Lavine, F. Zhu
BULLETIN OF ENTOMOLOGICAL RESEARCH
(2018)
Article
Developmental Biology
Hiroki Gotoh, Robert A. Zinna, Yuki Ishikawa, Hitoshi Miyakawa, Asano Ishikawa, Yasuhiro Sugime, Douglas J. Emlen, Laura C. Lavine, Toru Miura
DEVELOPMENTAL BIOLOGY
(2017)