Article
Biochemistry & Molecular Biology
Yuanxue Yang, Aiyu Wang, Yun Zhang, Chao Xue, Ming Zhao, Jianhua Zhang
Summary: Frequent use of insecticide can lead to environmental hazards and insecticide resistance in pests. This research identifies three endogenous microRNAs that are necessary for the small brown planthopper to develop tolerance to triflumezopyrim, an insecticide. These microRNAs activate metabolic detoxification pathways and interact with specific genes and proteins involved in resistance. Understanding these mechanisms is crucial for delaying the development of insecticide resistance.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Physiology
Shengfang Wen, Chang Liu, Xueting Wang, Youwei Wang, Chao Liu, Jinhua Wang, Xiaoming Xia
Summary: The study showed that continuous use of TFM can increase resistance of SBPH to TFM, but the resistance level can gradually decrease after stopping use. The resistant strains did not show cross-resistance to other neonicotinoid insecticides, but the increased activity of P450 enzyme may be due to the overexpression of P450 genes.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Virology
Gang Lu, Xiao-Di Zhang, Zhong-Tian Xu, Zhuang-Xin Ye, Yan Zhang, Jian-Ping Chen, Chuan-Xi Zhang, Jun-Min Li
Summary: A novel reovirus-like virus named Laodelphax striatellus reovirus (LSRV) was identified in the small brown planthopper, L. striatellus. LSRV was widely distributed in various tissues of the planthopper, with the highest expression in adult heads. However, LSRV was unable to replicate in rice plants. Host antiviral RNA interference pathway was found to be involved in LSRV infection. LSRV may be considered as a new species of the genus Fijivirus in the order Reovirales.
Article
Entomology
Yubiao Cai, Tao Dou, Futao Gao, Guanghua Wang, Yachang Dong, Nan Song, Shiheng An, Xinming Yin, Xiangyang Liu, Yingdang Ren
Summary: This study evaluated the sublethal effects of thiamethoxam on the small brown planthopper and found that sublethal concentrations of thiamethoxam can inhibit population growth and enhance detoxification enzyme activities.
JOURNAL OF ECONOMIC ENTOMOLOGY
(2022)
Article
Agriculture, Multidisciplinary
Wenxuan Huang, Yan Huo, Shaochen Yang, Mingjia Liu, Han Li, Man Zhang
Summary: In this paper, an improved YOLOv5s algorithm was proposed to implement the recognition and counting system of Laodelphax striatellus (SBPH). The model was enhanced by adding channel attention and spatial attention, as well as incorporating the Convolutional Block Attention Module (CBAM) and Adaptively Spatial Feature Fusion (ASFF). Experimental results showed that the improved model had a similar volume to YOLOv5s, but achieved approximately 4% higher mAP than YOLOv5s and 2% higher mAP than YOLOv5x. Additionally, an APP was developed using Android Studio to enable mobile image recognition and counting.
COMPUTERS AND ELECTRONICS IN AGRICULTURE
(2023)
Article
Agronomy
Shuirong Zhang, Fuchuan Gu, Ying Du, Xuyang Li, Changwei Gong, Jian Pu, Xuemei Liu, Xuegui Wang
Summary: Continuous selection of Laodelphax striatellus on triflumezopyrim resulted in the development of resistance, which did not lead to cross-resistance to other insecticides. The up-regulation of multiple P450 genes may play a role in mediating triflumezopyrim resistance in L. striatellus.
PEST MANAGEMENT SCIENCE
(2022)
Article
Entomology
Jie Chen, Meng-Ke Wang, Qi-Xian Xie, Xiao-Li Bing, Tong-Pu Li, Xiao-Yue Hong
Summary: The endosymbiont Wolbachia manipulates host reproduction through various strategies, including cytoplasmic incompatibility (CI). This study explored the potential rescue mechanism of CI in the small brown planthopper Laodelphax striatellus infected with Wolbachia. Comparative transcriptome analysis revealed differentially expressed genes, and RNA interference of specific Wolbachia-upregulated genes reduced egg reproduction. The upregulated gene NDUFA8 was associated with the rescue phenotype, suggesting it as a potential pest control target.
Article
Biochemistry & Molecular Biology
Marta Nowak, Katarzyna Zawadzka, Janusz Szemraj, Aleksandra Goralczyk-Binkowska, Katarzyna Lisowska
Summary: This study investigated the biodegradation mechanism of chloroxylenol by fungal strains and analyzed the ecotoxicity of emerging by-products. The results showed a high elimination rate of PCMX by the tested microorganisms, and the identification of new fungal by-products. Furthermore, the study revealed a detoxifying nature of the biodegradation process of PCMX.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Entomology
Amir Zaman Shah, Chao Ma, Yuanyuan Zhang, Qiuxin Zhang, Gang Xu, Guoqing Yang
Summary: This study demonstrates that the use of decoyinine as a seed treatment significantly reduces the fecundity of the small brown planthopper (SBPH) and improves rice resistance. Decoyinine enhances rice defense against SBPH by stimulating plant defensive responses, making it a potential environmentally friendly alternative to chemical pest control.
Article
Agronomy
Shengfang Wen, Yannan Xue, Ruoshi Du, Chang Liu, Xueting Wang, Youwei Wang, Chao Liu, Sheng Wang, Jinhua Wang, Xiaoming Xia
Summary: The study found that sublethal doses of TFM significantly affected the life table parameters, oviposition, and fecundity of SBPH, as well as the emergence rate of adults. In addition, insects treated with sublethal doses of TFM had longer developmental durations and adult life spans.
Article
Entomology
Yannan Xue, Chang Liu, Dongmei Liu, Wenjuan Ding, Zhaoge Li, Junli Cao, Xiaoming Xia
Summary: This study investigated the sensitivity of Laodelphax striatellus populations in different areas of Shandong, China to insecticides. The results showed that while some populations developed resistance to certain insecticides, all populations were sensitive to clothianidin, nitenpyram, and triflumezopyrim. The study also explored the biochemical characteristics and gene expression of the populations. These findings provide valuable information for the management strategies of L. striatellus in the field.
Article
Biochemistry & Molecular Biology
Yao Li, Yunye Zhang, Yin Xiang, Danyu Chen, Jia Hu, Fang Liu
Summary: Tissue-specific changes in gene expression profiles were greater than sex-specific changes in response to RSV infection in small brown planthoppers. Olfactory related genes in viruliferous antennae and legs showed significant differences compared to nonviruliferous ones, while differences in heads were minimal. The findings provide insights into controlling plant virus transmission and pest management.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Entomology
Haitao Wang, Yan Dong, Baijie Wan, Yinghua Ji, Qiufang Xu
Summary: Transient receptor potential mucolipin (TRPML) protein is crucial for Ca2+ ions release and plays roles in membrane trafficking, autophagy and ion homeostasis. In this study, we identified and characterized the TRPML protein in the small brown planthopper Laodelphax striatellus, revealing its evolutionary conservation and predominant expression in the ovary. The localization of Ls-TRPML in different cellular compartments and its lipid-binding characteristics were also elucidated, providing insights into the functions of TRPML in agricultural pests.
Article
Multidisciplinary Sciences
Jing Zhao, Jie Yang, Lili Zhang, Rongxiang Fang, Yan Huo
Summary: The study introduced a convenient method for researching insect saliva, collecting saliva-containing diet for further analysis by allowing insects to feed on artificial diet and filtering and concentrating the collected diet. The method detected the presence of virus and protein in insect saliva, laying a foundation for further research on factors related to feeding behavior and virus transmission.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2021)
Article
Plant Sciences
Muhammad Hafeez, Xiaowei Li, Limin Chen, Farman Ullah, Jun Huang, Zhijun Zhang, Jinming Zhang, Junaid Ali Siddiqui, Shu-xing Zhou, Xiao-yun Ren, Muhammad Imran, Mohammed A. Assiri, Yonggen Lou, Yaobin Lu
Summary: This study characterized and analyzed the detoxification gene CYP302A1 in the fall armyworm (FAW) and found higher expression levels in midgut tissue and older larvae. Increased P450 activity in the midgut of FAW larvae after exposure to rice plants was observed, as well as higher mortality in larvae treated with PBO followed by exposure to rice plants. Additionally, downregulation of the CYP302A1 gene, along with higher mortality, reduced larval weight, and shorter developmental time, was observed in dsRNA-fed larvae exposed to rice plants. Results suggest that the inducible P450 enzyme system and related genes enable herbivores to adapt to diverse host plants by utilizing secondary compounds.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Evolutionary Biology
Lei Chen, Jing-Tao Sun, Peng-Yu Jin, Ary A. Hoffmann, Xiao-Li Bing, Dian-Shu Zhao, Xiao-Feng Xue, Xiao-Yue Hong
EVOLUTIONARY APPLICATIONS
(2020)
Article
Biochemistry & Molecular Biology
X. -T. Yan, Z. -X. Ye, X. Wang, C. -X. Zhang, J. -P. Chen, J. -M. Li, H. -J. Huang
Summary: The study found that small brown planthopper has the best adaptation on wheat plants, followed by white-backed planthopper, while brown planthopper performs the worst. The transcriptomic responses of white-backed planthopper to rice and wheat plants show significant differences. There is varied bacterial diversity among the three planthoppers, but no dramatic change in response to different host plants.
INSECT MOLECULAR BIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Shi-Mei Huo, Zhi-Chao Yan, Feng Zhang, Lei Chen, Jing-Tao Sun, Ary A. Hoffmann, Xiao-Yue Hong
Summary: Our study revealed that different forms of T. urticae exhibit distinct host-specific transcription responses, and there is form-dependent plasticity in their transcriptomic responses. Genes associated with saliva, digestion, and detoxification showed form-dependent responses to the same host, with host-specific expression effects. Fitness differences on hosts are influenced by both color form and other population differences.
Article
Biology
Hai-Jian Huang, Xiao-Tian Yan, Zhong-Yan Wei, Yi-Zhe Wang, Jian-Ping Chen, Jun-Min Li, Zong-Tao Sun, Chuan-Xi Zhang
Summary: The bean bug, Riptortus pedestris, is a notorious pest of soybean crops in Asia, secreting a mixture of salivary proteins that induce cell death and plant defense responses. A total of 136 salivary proteins were identified in this study using transcriptomic and proteomic approaches, shedding light on the molecular mechanisms underlying plant-insect interactions. This research provides valuable insights for future studies on bug salivary effectors and pest management.
Article
Evolutionary Biology
Xin-Chao Ban, Zi-Kai Shao, Li-Jun Wu, Jing-Tao Sun, Xiao-Feng Xue
Summary: Arachnida is a diverse class in Arthropoda, and the monophyly of ten orders is confirmed while the monophyly of Acari and Arachnida is rejected. Highly rearranged mitochondrial genomes are found in mites, indicating their exceptional diversification. Arachnid orders have ancient origins and significantly diversified since the Cretaceous.
Article
Agronomy
Xin-Yu Zhou, Yi Ding, Jia-Yi Zhou, Kang-Kang Sun, Keiichiro Matsukura, Hui Zhang, Lei Chen, Xiao-Yue Hong, Jing-Tao Sun
Summary: This study provides genetic evidence of transoceanic migration of Laodelphax striatellus from China to Japan and highlights the significance of the X chromosome in deciphering migration trajectories with low genetic differentiation.
PEST MANAGEMENT SCIENCE
(2022)
Article
Ecology
Hong-Yuan Wei, Yu-Xuan Ye, Hai-Jian Huang, Ming-Shun Chen, Zi-Xiang Yang, Xiao-Ming Chen, Chuan-Xi Zhang
Summary: The study generated a high-quality genome assembly of the aphid Schlechtendalia chinensis, providing a solid genetic foundation for understanding gall formation and the interaction between aphids and their host plants.
ECOLOGY AND EVOLUTION
(2022)
Article
Entomology
Li-Li Li, Zhuang-Xin Ye, Jian-Ping Chen, Chuan-Xi Zhang, Hai-Jian Huang, Jun-Min Li
Summary: This study revealed the full genomes of two insect-specific viruses (ISVs) in green leafhoppers and investigated the host antiviral RNA interference (RNAi) response. The findings provide valuable information on ISVs in leafhoppers and may have practical applications in pest management.
Article
Virology
Yi-Zhe Wang, Zhuang-Xin Ye, Jian-Ping Chen, Chuan-Xi Zhang, Jun-Min Li, Hai-Jian Huang
Summary: A novel iflavirus named Lycorma delicatula iflavirus 1 (LDIV1) was identified in the spotted lanternfly. LDIV1 replicates in the host insect and activates host antiviral immunity. Phylogenetic analysis showed that LDIV1 is a new species in the genus Iflavirus.
ARCHIVES OF VIROLOGY
(2022)
Article
Entomology
Qi-Qi Hu, Xin-Yue Yu, Xiao-Feng Xue, Xiao-Yue Hong, Jian-Ping Zhang, Jing-Tao Sun
Summary: This study found that spider mites cannot locate suitable host plants from a long distance by volatile odours, but they can use a combination of olfactory and gustatory senses to select suitable host plants at a short distance. The similarity in perceiving suitable host plants among spider mite species is highly correlated with their phylogenetic relationships.
Article
Entomology
Yue Huang, Biao Hu, Zhongyan Wei, Shiqi Shan, Chunyun Guo, Hehong Zhang, Yanjun Li, Jianping Chen, Xue Kang, Haijian Huang, Zongtao Sun
Summary: R. pedestris is a major pest in soybeans causing staygreen syndrome, and recent research suggests that its salivary proteins may play a crucial role in insect infestation. This study identified Rp2155 as a salivary effector protein that induces cell death and is specifically expressed in the salivary gland of R. pedestris. It was found that silencing Rp2155 can alleviate soybean staygreen symptoms caused by R. pedestris, suggesting that it could be a potential target for insect control.
Article
Agronomy
Hai-Bin Lu, Jia-Bao Lu, Li-Li Li, Ze-Long Zhang, Jian-Ping Chen, Jun-Min Li, Chuan-Xi Zhang, Hai-Jian Huang
Summary: This study identifies 69 neutral lipases in the bean bug, R. pedestris, and analyzes their functions. The results show that several of these lipases play crucial roles in insect reproduction and survival, suggesting their potential as targets for controlling this pest.
PEST MANAGEMENT SCIENCE
(2023)
Article
Genetics & Heredity
Xin Wang, Zhuang-Xin Ye, Yi-Zhe Wang, Xiao-Jing Wang, Jian-Ping Chen, Hai-Jian Huang
Summary: This study investigates the gene expression patterns of tobacco in response to whitefly infestation. It finds that whitefly infestation activates plant defense mechanisms, allowing the plant to recognize pathogens and enhance immunity. However, it also suppresses energy metabolism, carbohydrate metabolism, ribosomes, and photosynthesis, leading to impaired plant development.
Article
Entomology
Ze-Long Zhang, Xiao-Jing Wang, Hai-Bin Lu, Hai-Jian Huang
Summary: This study investigated the gut transcriptomic changes of Riptortus pedestris when feeding on different parts of soybean plants, revealing the mechanisms by which they adapt to different food resources.
Article
Multidisciplinary Sciences
Hai-Jian Huang, Yi-Yuan Li, Zhuang-Xin Ye, Li-Li Li, Qing-Ling Hu, Yu-Juan He, Yu-Hua Qi, Yan Zhang, Ting Li, Gang Lu, Qian-Zhuo Mao, Ji-Chong Zhuo, Jia-Bao Lu, Zhong-Tian Xu, Zong-Tao Sun, Fei Yan, Jian-Ping Chen, Chuan-Xi Zhang, Jun-Min Li
Summary: Non-retroviral endogenous viral elements (nrEVEs) are widely dispersed in eukaryotic genomes, with their functions largely unknown. In this study, researchers discovered that a ToEVE in planthoppers has been domesticated and plays essential roles in their development and reproduction. Analysis of arthropod genomes suggests that nrEVEs may contribute to the functional diversity of arthropod genes.
NATURE COMMUNICATIONS
(2023)
