Review
Chemistry, Inorganic & Nuclear
Valerie Waser, Thomas R. Ward
Summary: This review examines the effect of aqueous media on the stability and redox chemistry of biomimetic analogs and artificial [Fe4S4] proteins.
COORDINATION CHEMISTRY REVIEWS
(2023)
Review
Plant Sciences
Bing Yang, Chenyun Xu, Yuting Cheng, Ting Jia, Xueyun Hu
Summary: Iron-sulfur (Fe-S) clusters are ancient protein cofactors that exist ubiquitously in organisms and play important roles in various life processes. Plastids, semi-autonomous organelles believed to originate from cyanobacterial endosymbionts, have a Fe-S cluster biosynthesis and delivery pathway similar to cyanobacteria. Fe-S clusters are crucial for the normal functioning of downstream Fe-S proteins in plastids. Recent research has made significant progress in understanding this pathway and this review summarizes these findings as well as the remaining scientific challenges.
PLANT CELL REPORTS
(2023)
Article
Biotechnology & Applied Microbiology
Maria Florencia Pignataro, Maria Georgina Herrera, Natalia Brenda Fernandez, Martin Aran, Hernan Gustavo Gentili, Fernando Battaglini, Javier Santos
Summary: In this study, a small protein, Affi_224, was successfully screened using ribosome display method. Affi_224 showed a high affinity for Frataxin and was able to increase the activity of Cys NFS1 desulfurase caused by the FRDA Frataxin variant G130V. The interaction between Affi_224 and Frataxin was also observed in a human cellular model. This research suggests that quaternary addition could be a new tool for modulating Frataxin function.
BIOTECHNOLOGY AND BIOENGINEERING
(2023)
Article
Multidisciplinary Sciences
Jae-Hun Jeoung, Sabine Nicklisch, Holger Dobbek
Summary: Metalloenzymes coupled with ATP hydrolysis can catalyze reductions at very negative reduction potentials. The study found that the DCCP:DCCP-R complex forms stable complexes and facilitates ATP-driven electron transfer through structural changes and hydrogen bond networks.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Jing Du, Zhaoyang Huang, Yanchun Li, Xueying Ren, Chaoting Zhou, Ruolan Liu, Ping Zhang, Guojie Lei, Jianxin Lyu, Jianghui Li, Guoqiang Tan
Summary: Copper is a necessary mineral nutrient but excess copper is cytotoxic. Wilson's disease is an autosomal recessive hereditary disease characterized by pathological copper accumulation in organs. The molecular mechanism of Wilson's disease is not fully understood and further research is needed to develop therapeutic strategies.
FREE RADICAL BIOLOGY AND MEDICINE
(2023)
Article
Chemistry, Multidisciplinary
Youngsuk Kim, Arun Sridharan, Daniel L. M. Suess
Summary: This study reports the synthesis of a three-member series of isostructural mononitrosylated [Fe4S4] clusters and reveals the significance of mononitrosylated intermediates in NO sensing/signaling.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Biochemistry & Molecular Biology
Thomas Roret, Bo Zhang, Anna Moseler, Tiphaine Dhalleine, Xing-Huang Gao, Jeremy Couturier, Stephane D. Lemaire, Claude Didierjean, Michael K. Johnson, Nicolas Rouhier
Summary: This study examined the catalytic capacity of Chlamydomonas reinhardtii GRX2 recombinant protein and unexpectedly found the presence of a [2Fe-2S] cluster in the protein purified under anaerobiosis, despite previous mutational analyses suggesting an inadequate active site signature. These results indicate the existence of an atypical Fe-S cluster environment and ligation mode in conventional GRXs, highlighting the complexity of factors influencing their Fe-S binding capacity.
Article
Biochemistry & Molecular Biology
Jing Zhang, Zechen Bai, Min Ouyang, Xiumei Xu, Haibo Xiong, Qiang Wang, Bernhard Grimm, Jean-David Rochaix, Lixin Zhang
Summary: Fe-S clusters are ancient and important for life processes. The biogenesis of Fe-S clusters involves iron acquisition, sulfur mobilization, and cluster formation. Two Arabidopsis DnaJ proteins, DJA6 and DJA5, play a crucial role in facilitating iron incorporation into Fe-S clusters during chloroplast Fe-S cluster biogenesis. Loss of these proteins leads to defects in chloroplast Fe-S protein accumulation and photosynthesis dysfunction. Evolutionary analysis shows that DJA6 and DJA5 are highly conserved in photosynthetic organisms and have a strong evolutionary relationship with SUFE1, SUFC, and SUFD.
Review
Chemistry, Inorganic & Nuclear
Jason C. Crack, Nick E. Le Brun
Summary: Iron-sulfur (FeS) clusters are protein cofactors essential for various biological functions such as electron transfer, catalysis, and gene regulation. Recent progress in using mass spectrometry has provided new insights into FeS cluster assembly and chemistry, particularly in transcriptional regulators coordinating cellular responses to changing conditions.
COORDINATION CHEMISTRY REVIEWS
(2021)
Article
Chemistry, Multidisciplinary
Soosan Hosseinmardi, Andreas Scheurer, Frank W. Heinemann, Nicola Marigo, Dominik Munz, Karsten Meyer
Summary: This study reports a two-step protonation reaction from a Ni-0 precursor to dihydrogen, with the formation of a rare nickel dihydrogen complex in the second protonation step.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Article
Chemistry, Organic
Zhanqun Liang, Kang Lv, Shaofang Zhou, Changlei Zhu, Xiaoguang Bao
Summary: A protocol for synthesizing trisubstituted alkenyl thioethers through visible-light photocatalysis is disclosed, with high stereoselectivity achieved in the presence of Cu(OAc)2 catalyst. Mechanistic studies suggest different quenching pathways of the excited photocatalyst with and without Cu(OAc)2 catalyst, influencing the product selectivity.
ORGANIC CHEMISTRY FRONTIERS
(2021)
Review
Oncology
Jaewang Lee, Jong-Lyel Roh
Summary: Iron dysregulation is a characteristic of cancer, characterized by increased expression of genes involved in iron metabolism and iron-sulfur cluster (ISC) biogenesis. Dysregulated iron homeostasis leads to elevated intracellular labile iron, which can result in the formation of excessive cytotoxic radicals and susceptibility to regulated cell death, including ferroptosis. Various strategies, such as redox operations, iron chelation, and replacement of iron with redox-inert metals, can destabilize or restrict ISC formation and function, providing potential therapeutic approaches for cancer treatment. Targeting ISCs to induce ferroptosis shows promise in cancer therapy. This review provides a comprehensive overview of iron metabolism, ferroptosis, and the role of ISC modulation in cancer cells, as well as the potential of targeting ISCs for ferroptosis induction in cancer treatment. Further research and clinical trials are needed to validate these strategies for various cancers, with the goal of developing novel and effective cancer treatments.
Article
Chemistry, Organic
Florian J. Ritz, Lars Valentin, Anja Henss, Christian Wuertele, Olaf Walter, Sergei I. Kozhushkov, Armin de Meijere, Siegfried Schindler
Summary: The study investigated the kinetics of reactions between [Ni(bipy)(COD)] and various bicyclopropylidene compounds using stopped-flow techniques. Results supported an associative mechanism and allowed for a more general reaction pathway to be postulated. The structurally characterized nickel(0) and copper(I) complexes exhibited reactivity towards dioxygen, and comparisons were made with similar olefinic complexes in the literature.
EUROPEAN JOURNAL OF ORGANIC CHEMISTRY
(2021)
Review
Chemistry, Inorganic & Nuclear
Amy E. E. Boncella, Emily T. T. Sabo, Robert M. M. Santore, Jens Carter, Jaclyn Whalen, Jesse D. D. Hudspeth, Christine N. N. Morrison
Summary: This review provides an overview of the versatile applications of Fe-S clusters in and beyond biology, discussing the structure-function relationship of Fe-S clusters in different research areas and analyzing trends and connections in Fe-S cluster research.
COORDINATION CHEMISTRY REVIEWS
(2022)
Review
Biochemistry & Molecular Biology
Naoki Shigi
Summary: Sulfur-containing biomolecules play crucial roles in cells, including enzyme cofactors, RNA composition, and regulation of gene translation efficiency. Modulating sulfur modification allows cells to adapt to different environments. Mutations in related genes can lead to diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)