Article
Surgery
Chandan K. Sen, Sashwati Roy, Shomita S. Mathew-Steiner, Gayle M. Gordillo
Summary: Biofilm infection is a major challenge in wound care as biofilms protect microorganisms from antimicrobial therapies and host defenses. Treatment often involves systemic antimicrobials and debridement of wounds to healthy tissue, but visualizing biofilms remains difficult. Limited impact has been seen with current therapies, and decisions on commercial products should consider mechanism of action and experimental system data.
PLASTIC AND RECONSTRUCTIVE SURGERY
(2021)
Article
Dermatology
Franziska Rademacher, Joachim Bartels, Regine Glaser, Meno Rodewald, Sabine Schubert, Daniel Drucke, Holger Rohde, Jurgen Harder
Summary: Staphylococcus epidermidis (SE) plays an important role in skin defense by activating IL-1 signaling. A serine protease called Esp produced by SE was found to be crucial in the release of mature IL-beta in a caspase-1-independent manner.
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2022)
Article
Pharmacology & Pharmacy
Joop J. P. Kouijzer, Kirby R. Lattwein, Ines Beekers, Simone A. G. Langeveld, Mariel Leon-Grooters, Jean-Marc Strub, Estefania Oliva, Gaetan L. A. Mislin, Nico de Jong, Antonius F. W. van der Steen, Alexander L. Klibanov, Willem J. B. van Wamel, Klazina Kooiman
Summary: The study confirmed the successful incorporation of vancomycin into microbubble lipid shell and demonstrated that vancomycin-decorated microbubbles significantly bound to biofilms and remained bound under shear stress conditions. Upon ultrasound exposure, the area of biofilm was reduced by up to 28%, supporting the potential of vancomycin-decorated microbubbles as a new theranostic tool for S. aureus biofilm infections allowing for specific bacterial detection and disruption.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2021)
Article
Biochemistry & Molecular Biology
Biao Li, Ling Zhang, Li Wang, Yong Wei, Jiuqiang Guan, Qundi Mei, Ninghao Hao
Summary: This study reports the antimicrobial activity of yak beta-defensin 116 against Staphylococcus aureus and its application in diarrheal disease. The findings suggest that DEFB116 protein can disrupt bacterial cell membrane, kill S. aureus, and improve histopathological features in a mouse model of diarrhea. Additionally, DEFB116 protein increases the abundance of beneficial bacteria and inhibits the growth of pathogenic bacteria.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biology
Neil Zhao, Dylan Curry, Rachel E. E. Evans, Selin Isguven, Theresa Freeman, John R. R. Eisenbrey, Flemming Forsberg, Jessica M. M. Gilbertie, Sophie Boorman, Rachel Hilliard, Sana S. S. Dastgheyb, Priscilla Machado, Maria Stanczak, Marc Harwood, Antonia F. F. Chen, Javad Parvizi, Irving M. M. Shapiro, Noreen J. J. Hickok, Thomas P. P. Schaer
Summary: Coupling ultrasound-triggered microbubble destruction with antibiotics in diluted synovial fluid effectively clears S. aureus in septic arthritis models. Treatment failure in joint infections is associated with antibiotic-resistant Staphylococcus aureus aggregates formed in synovial fluid. In vitro and porcine model experiments show that ultrasound-triggered microbubble destruction enhances antibiotic activity against Staphylococcus aureus aggregates when synovial fluid is diluted.
COMMUNICATIONS BIOLOGY
(2023)
Article
Acoustics
Jinyue Sun, Debao Wang, Zhilan Sun, Fang Liu, Lihui Du, Daoying Wang
Summary: The study demonstrated that the combined treatment of ultrasound and chlorogenic acid exhibited significant synergistic antibacterial effects against Staphylococcus aureus, causing severe cell damage, decreased membrane permeability, release of intracellular substances, and reduced exopolysaccharide content in bacterial biofilms.
ULTRASONICS SONOCHEMISTRY
(2021)
Article
Cell Biology
Hanjun Zhao, Kelvin Kai-Wang To, Hoiyan Lam, Chuyuan Zhang, Zheng Peng, Xinjie Meng, Xiankun Wang, Anna Jinxia Zhang, Bingpeng Yan, Jianpiao Cai, Man Lung Yeung, Jasper Fuk-Woo Chan, Kwok-Yung Yuen
Summary: The human-derived peptide 4H30 demonstrates broad antiviral activity against SARS-CoV-2 and its variants, inhibiting viral entry, fusion, and release.
Article
Multidisciplinary Sciences
Anshuman Dasgupta, Tao Sun, Roberto Palomba, Elena Rama, Yongzhi Zhang, Chanikarn Power, Diana Moeckel, Mengjiao Liu, Apoorva Sarode, Marek Weiler, Alessandro Motta, Celine Porte, Zuzanna Magnuska, Asmaa Said Elshafei, Roman Barmin, Adam Graham, Arthur McClelland, Dirk Rommel, Elmar Stickeler, Fabian Kiessling, Roger M. Pallares, Laura De Laporte, Paolo Decuzzi, Nathan McDannold, Samir Mitragotri, Twan Lammers
Summary: Surface tension allows microbubbles (MB) to have a perfect spherical shape. By stretching spherical poly(butyl cyanoacrylate) MB above their glass transition temperature, anisotropic MB can be generated, giving them unique features for biomedical applications. Compared to spherical MB, nonspherical polymeric MB exhibited superior performance including increased margination behavior, reduced macrophage uptake, prolonged circulation time, and enhanced blood-brain barrier permeation when combined with transcranial focused ultrasound (FUS). The study identifies shape as a design parameter in the MB landscape and provides a rational and robust framework for exploring the application of anisotropic MB in ultrasound-enhanced drug delivery and imaging applications.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Radiology, Nuclear Medicine & Medical Imaging
Qiong Zhu, Xiaoxun Xu, Beibei Chen, Yiyi Liao, Xue Guan, Ying He, Hai Cui, Yani Rong, Zheng Liu, Yali Xu
Summary: This study explored the feasibility, efficacy, and safety of ultrasound-targeted microbubbles destruction-assisted dual delivery of beta-amyloid antibody and neural stem cells for Alzheimer's disease. The results showed that the dual delivery treatment improved memory and spatial learning, promoted Aβ plaques clearance, and increased BDNF expression, providing potential insight for AD treatment.
Article
Engineering, Biomedical
Yunfan Pan, Yongjian Li, Yan Li, Xiaobing Zheng, Chenghong Zou, Jiang Li, Haosheng Chen
Summary: Thrombosis is a critical medical condition that can result in severe consequences. This study proposes a novel approach called sonothrombolysis, which uses nanodroplet-coated microbubbles and a two-step cavitation strategy to accelerate the dissolution of blood clots. The results demonstrate that this method shows promise in improving thrombolytic efficiency and can serve as an effective drug carrier for targeted drug delivery.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Food Science & Technology
Hui Yang, Xiangjun Zhan, Luyi Song, Shuai Cheng, Ruiying Su, Yingying Zhang, Du Guo, Xin Lu, Xiaodong Xia, Chao Shi
Summary: This study investigated the antibacterial and antibiofilm mechanism of ultrasound combined with citral nanoemulsion against Staphylococcus aureus and mature biofilm. The combination treatment disrupted cell membrane integrity and permeability, enhanced cellular oxidative stress and membrane lipid peroxidation, and resulted in cell rupture and collapse. In addition, the treatment showed a more pronounced removal effect than both alone in the biofilm on the stainless steel sheet. This research provides a safe and efficient sterilization method for the food industry.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Martynas Maciulevicius, Diana Navickaite, Sonam Chopra, Baltramiejus Jakstys, Saulius Satkauskas
Summary: Research has shown that different calcium concentrations affect cell membrane permeability and cell viability during sonoporation, with ultrasound-microbubble cavitation playing a significant role. The study also indicates that inertial cavitation activities are directly involved in calcium delivery via sonoporation, leading to rapid cell death.
Article
Food Science & Technology
Hang Yu, Yang Liu, Fangwei Yang, Yunfei Xie, Yahui Guo, Yuliang Cheng, Weirong Yao
Summary: The combination of high-intensity ultrasound and chlorine dioxide treatment showed synergistic effects in inactivating viable cells and clearing Staphylococcus aureus biofilms, with the ultrasound promoting penetration and diffusion of ClO2. The treatment also reduced biofilm formation and toxin secretion, leading to a decrease in sublethal cells. Surface properties of food contact materials, particularly roughness, negatively correlated with biofilm clearance efficiency after the combined treatment, suggesting the need for further evaluation.
Article
Chemistry, Multidisciplinary
Benjamin van Elburg, Joke Deprez, Martin van den Broek, Stefaan C. De Smedt, Michel Versluis, Guillaume Lajoinie, Ine Lentacker, Tim Segers
Summary: Sonoporation is a process facilitated by ultrasound-driven microbubble oscillations that enhances intracellular drug delivery. This study aimed to investigate the role of microbubble size on sonoporation and the relevant mechanism(s). The results showed that sonoporation efficiency strongly depended on the equilibrium bubble size, with bubbles of approximately 4.7 μm radius being the most efficient. Shear stress was unlikely to be the mechanism of action, while normal stress correlated well with sonoporation efficiency.
JOURNAL OF CONTROLLED RELEASE
(2023)
Review
Biochemistry & Molecular Biology
Vincent Jean-Pierre, Agathe Boudet, Pauline Sorlin, Quentin Menetrey, Raphael Chiron, Jean-Philippe Lavigne, Helene Marchandin
Summary: Staphylococcus aureus is a major human pathogen that can survive and cause diseases in various clinical settings, including Cystic Fibrosis. In Cystic Fibrosis, S. aureus is the most commonly identified opportunistic pathogen, and its colonization/infection can become chronic in about one third of patients. S. aureus's ability to form biofilm is a crucial factor contributing to its persistence and protection in the lungs of CF patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Youcui Xu, Jing Chen, Wei Jiang, Yangyang Zhao, Chen Yang, Yi Wu, Qianming Li, Chen Zhu
Summary: A novel multifunctional nanodrug CD-Dex is developed targeting pathological microstructures, scavenging ROS, and suppressing inflammation, showing great potential in liver fibrosis therapy by eliminating ROS and inhibiting inflammation.
Article
Surgery
Jiale Dong, Lingtong Kong, Siming Zhang, Xifu Shang, Jiaxing Wang, Xianzuo Zhang, Chen Zhu
Summary: This study compared early clinical outcomes of direct anterior and posterolateral THA in hip fusion treatment. The DAA group showed better Harris and OHS scores at 1 and 3 months postoperatively. Additionally, DAA group had greater cup anteversion angle and more successful orientation in both inclination and anteversion angles compared to the PLA group.
FRONTIERS IN SURGERY
(2022)
Article
Materials Science, Multidisciplinary
Wufei Ge, Kai Chen, Hongyan Tang, Xahriyar Arken, Xianzuo Zhang, Xuenan Gu, Chen Zhu
Summary: The study demonstrates that Mg-Ca-La alloys with trace La addition exhibit good biocompatibility and stable degradation rate for orthopedic applications. Animal experiments show that implantation of this alloy near bones can promote bone growth without significant adverse effects.
Article
Biotechnology & Applied Microbiology
Jiawei Mei, Jun Zhou, Lingtong Kong, Yong Dai, Xianzuo Zhang, Wenqi Song, Chen Zhu
Summary: In this study, a photocurable hydrogel system was developed to enhance diabetic-wound healing through spatiotemporal immunomodulation. The system effectively resolved the immune contradiction in diabetic wounds by releasing drugs and silver nanoparticles, promoting tissue repair while inhibiting bacterial aggregation.
JOURNAL OF NANOBIOTECHNOLOGY
(2022)
Review
Orthopedics
Wei Zhou, Meng Li, Ruixiang Ma, Gang Yao, Chen Zhu, Guang Chen
Summary: This case report describes a rare tibial plateau fracture with an isolated bone fragment. Combining the physical examination and radiological findings to evaluate the potential injury mechanism is important for developing an appropriate surgical protocol.
ORTHOPAEDIC SURGERY
(2022)
Article
Rheumatology
Jiajie Tu, Weile Chen, Yilong Fang, Dafei Han, Yizhao Chen, Haifeng Jiang, Xuewen Tan, Zhen Xu, Xuming Wu, Huihui Wang, Xiangling Zhu, Wenming Hong, Zhenbao Li, Chen Zhu, Xinming Wang, Wei Wei
Summary: This study investigates the function and mechanism of PU.1, a crucial transcription factor, in the development of rheumatoid arthritis (RA). The results demonstrate the role of PU.1 in RA and suggest that targeting PU.1 could be a potential therapeutic approach by repressing FLT3.
ANNALS OF THE RHEUMATIC DISEASES
(2023)
Article
Engineering, Biomedical
Kai Chen, Wufei Ge, Li Zhao, Lingtong Kong, Hongtao Yang, Xianzuo Zhang, Xuenan Gu, Chen Zhu, Yubo Fan
Summary: This study reports a low-alloyed Zn-Mg-Ag material with optimized strength degeneration profiles, excellent antibacterial efficacy, and osteogenic activity. The Zn-0.05Mg-0.1Ag alloy showed favorable mechanical properties, high antibacterial performance, and promoted osteogenesis.
ACTA BIOMATERIALIA
(2023)
Article
Chemistry, Multidisciplinary
Jun Zhou, Jiawei Mei, Quan Liu, Dongdong Xu, Xiaoli Wang, Xianzuo Zhang, Wanbo Zhu, Chen Zhu, Jiaxing Wang
Summary: In this study, a novel spatiotemporal on-off immunomodulatory therapy (SOIT) is proposed for Biofilm infected diabetic wounds (BIDWs) through biphasic regulation of NLRP3. Methacrylate gelatin hydrogels (Gel-MA) incorporated with graphene oxide (GO) and metformin-loaded mesoporous silicone nanospheres are synthesized and used to construct a nanocomposite hydrogel (MGO@GM). GO nanosheets released from MGO@GM inhibit bacterial biofilm formation and activate NLRP3 to induce a macrophage-associated proinflammatory response. With the degradation of MGO@GM, released metformin reduces local hyperglycemia, downregulates NLRP3, and inhibits NETs formation. Repolarized M2 macrophages alleviate the inflammatory microenvironment and promote tissue regeneration. Overall, this SOIT strategy provides a new perspective for the future clinical treatment of BIDWs.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Engineering, Biomedical
Siming Zhang, Gaoran Ge, Yi Qin, Wenhao Li, Jiale Dong, Jiawei Mei, Ruixiang Ma, Xianzuo Zhang, Jiaxiang Bai, Chen Zhu, Weiwei Zhang, Dechun Geng
Summary: Poor wound healing after diabetes mellitus is characterized by persistent bleeding, inflammation dysregulation, blocked cell proliferation, infection susceptibility, and impaired tissue remodeling. Conventional wound dressings have limited functionality and fail to meet the requirements of diabetic wound healing. Stimuli-responsive hydrogels, which can change their properties in response to various stimuli, have shown promise in improving therapeutic efficacy and reducing toxicity for diabetic wound healing.
MATERIALS TODAY BIO
(2023)
Article
Chemistry, Multidisciplinary
Jiawei Mei, Dongdong Xu, Lingtian Wang, Lingtong Kong, Quan Liu, Qianming Li, Xianzuo Zhang, Zheng Su, Xianli Hu, Wanbo Zhu, Ming Ye, Jiaxing Wang, Chen Zhu
Summary: A BME-responsive copper-doped polyoxometalate clusters (Cu-POM) combination with mild photothermal therapy (PTT) and macrophage immune re-rousing is proposed for the eradication of bacterial biofilm-associated infections (BAIs) at all stages. Cu-POM self-assembles in the acidic biofilm microenvironment and converts endogenous H2O2 to toxic •OH through chemodynamic therapy (CDT), inducing bacterial metabolic exuberance and promoting bacterial cuproptosis-like death. The re-rousing macrophages scavenge escaping planktonic bacteria through enhanced chemotaxis and phagocytosis. Overall, BME-responsive Cu-POM promotes bacterial death and reactivates macrophage immune response, leading to all-stage BAI clearance.
ADVANCED MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Zhiqiang Shen, Shaoqiu Zheng, Yuanmeng Fang, Guoying Zhang, Chen Zhu, Shiyong Liu, Jinming Hu
Summary: We have successfully developed a novel strategy for constructing ONOO- donors by combining near-infrared-mediated type I photosensitization and photoredox catalysis. The design involves a Nile blue analogue that can serve as both a type I photosensitizer and a metal-free photocatalyst. The formation of O-2(center dot-) via type I photosensitization activates nitrobenzofurazan-based NO donors via oxygen-tolerant NIR photoredox catalysis, resulting in the simultaneous release of O-2(center dot-) and NO, leading to the release of ONOO- with antibacterial and antibiofilm activities.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Pharmacology & Pharmacy
Zheng Su, Lingtong Kong, Jiawei Mei, Qianming Li, Zhengzheng Qian, Yuanyuan Ma, Yue Chen, Shenghong Ju, Jiaxing Wang, Weitao Jia, Chen Zhu, Wenpei Fan
Summary: A novel non-antibiotic strategy based on the synergy of bionanocatalysts-driven heat-amplified chemodynamic therapy (CDT) and innate immunomodulation is proposed for specific biofilm elimination. The results show that this strategy can precisely eliminate refractory peri-implant biofilm infections with extremely few adverse effects.
DRUG RESISTANCE UPDATES
(2023)
Article
Chemistry, Multidisciplinary
Qianming Li, Quan Liu, Zhengxi Wang, Xianzuo Zhang, Ruixiang Ma, Xianli Hu, Jiawei Mei, Zheng Su, Wanbo Zhu, Chen Zhu
Summary: A novel strategy using bovine serum albumin-iridium oxide nanoparticles as biofilm homeostasis interrupter and immunomodulator, along with singlet oxygen-sensitized mild hyperthermia, is reported for combating biofilm-associated infections.
Review
Biotechnology & Applied Microbiology
Jiaxuan Sun, Wenzhi Wang, Xianli Hu, Xianzuo Zhang, Chen Zhu, Jinming Hu, Ruixiang Ma
Summary: In the past decade, nanoparticles have been extensively researched for delivering gaseous signaling molecules in medical applications. The role of gaseous signaling molecules and their local delivery through nanoparticles have shown great potential in diagnosing and treating orthopedic diseases, in addition to their application in oncology. This review highlights three recognized gaseous signaling molecules (nitric oxide, carbon monoxide, and hydrogen sulfide) and their distinct biological functions and roles in orthopedic diseases. Furthermore, the review summarizes the progress in therapeutic development over the past ten years, addressing unresolved issues and potential clinical applications.
JOURNAL OF NANOBIOTECHNOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Xianli Hu, Ruixiang Ma, Peng Zhang, Jiale Dong, Jiaxuan Sun, Wenzhi Wang, Quan Liu, Lingtong Kong, Xudong Zhang, Zhengxi Wang, Jiawei Mei, Xifu Shang, Wanbo Zhu, Zheng Su, Chen Zhu
Summary: This study developed a biomimetic nanosphere (USPM) that can be recruited to the biofilm microenvironment. By releasing selenite and combining with near-infrared-induced mild photothermal therapy, USPM can precisely disrupt the biofilm and bacterial metabolism. Additionally, USPM promotes the phagocytosis and autophagy of macrophages, enhancing the immune response. This method provides an alternative strategy to treat refractory biofilm-associated infections.
ADVANCED FUNCTIONAL MATERIALS
(2023)
