Article
Biochemistry & Molecular Biology
J. Allombert, C. Jaboulay, C. Michard, C. Andrea, X. Charpentier, A. Vianney, P. Doublet
Summary: The Legionella pneumophila Icm/Dot T4SS can translocate a large number of effectors, and research has found that their delivery is controlled by specific timing to effectively manipulate host cells. The timed delivery of effectors depends not only on chaperone protein interactions but also involves cyclic-di-GMP signaling.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Immunology
Ines P. Monteiro, Sofia Sousa, Vitor Borges, Paulo Goncalves, Joao Paulo Gomes, Luis Jaime Mota, Irina S. Franco
Summary: Two novel effectors, VFX05045 and VFX10045, of Legionella pneumophila were identified and their subcellular localizations were determined. Furthermore, the essential residues for nuclear targeting of VFX10045 were identified.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Biology
Michael J. Sheedlo, Clarissa L. Durie, Jeong Min Chung, Louise Chang, Jacquelyn Roberts, Michele Swanson, Dana Borden Lacy, Melanie D. Ohi
Summary: This passage discusses the relationship between Legionella pneumophila and Legionnaires' disease, as well as the structure of the Dot/Icm T4SS core complex determined by single particle cryo-EM. It identifies new proteins contributing to the core T4SS structure and defines two areas of symmetry mismatch.
Article
Multidisciplinary Sciences
Marietta S. Kaspers, Vivian Pogenberg, Christian Pett, Stefan Ernst, Felix Ecker, Philipp Ochtrop, Michael Groll, Christian Hedberg, Aymelt Itzen
Summary: In this study, the authors uncover the mechanism and substrate recognition of Legionella effector Lem3, which removes a phosphocholine moiety from the human protein Rab1 during infection. They present the crystal structure of the stabilised Lem3:Rab1b complex, revealing the catalytic mechanism and substrate recognition of PPM phosphatases shaped Lem3. This research is of significant importance for understanding bacterial pathogenesis and protein substrate recognition.
NATURE COMMUNICATIONS
(2023)
Review
Microbiology
Ashley M. Joseph, Stephanie R. Shames
Summary: This review discusses the function of metaeffectors in Legionella pneumophila, which regulate other effectors essential for pathogen replication. While there are at least a dozen known metaeffectors in L. pneumophila, their exact mechanisms of promoting virulence remain largely unknown. The review also highlights challenges in identifying and understanding the contribution of metaeffectors to bacterial pathogenesis.
Article
Biochemistry & Molecular Biology
Miles H. Black, Adam Osinski, Gina J. Park, Marcin Gradowski, Kelly A. Servage, Krzysztof Pawlowski, Vincent S. Tagliabracci
Summary: ADP-ribosyltransferases (ARTs) are a widespread superfamily of enzymes commonly used by bacteria in pathogenic strategies. Legionella pneumophila has a diverse set of 330 translocated effectors, but the ART effectors influencing this bacteria have not been well defined. The discovery of Legionella ADP-Ribosyltransferase 1 (Lart1) targeting a host metabolic enzyme during infection represents a novel mechanism of pathogenicity for this bacterium.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Xiaoli Zeng, Min Huang, Qing-Xue Sun, Ye-Jun Peng, Xiaomei Xu, Yun-Bin Tang, Ju-Yuan Zhang, Yiling Yang, Cheng-Cai Zhang
Summary: This study identified a c-di-GMP receptor called CdgR, which plays a crucial role in regulating cell size in cyanobacteria. CdgR interacts with a global transcription factor DevH and functions through c-di-GMP-mediated signaling. The findings provide insights into the mechanism of c-di-GMP signaling in controlling bacterial cell size.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Biochemistry & Molecular Biology
Sun Cheol Park, So Yeon Cho, Tae Hee Kim, Kyung Yeol Ko, Wan Seok Song, Seung Goo Kang, Geun-Shik Lee, Sung-il Yoon
Summary: LegK7 is an atypical kinase that activates through interaction with the host MOB1 protein without the need for phosphorylation. Upon binding with MOB1, LegK7 undergoes a structural reshaping that allows it to adopt a closed conformation compatible with ATP binding.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Review
Immunology
Tomoe Kitao, Tomoko Kubori, Hiroki Nagai
Summary: This review summarizes recent advances in the structural studies of the L. pneumophila IVB secretion system and provides insights into substrate recognition and transport mechanisms.
MICROBIOLOGY AND IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Ivy Y. W. Chung, Lei Li, Oleg Tyurin, Alla Gagarinova, Raissa Wibawa, Pengfei Li, Elizabeth L. Hartland, Miroslaw Cygler
Summary: Legionella pneumophila is an intracellular pathogen that causes Legionnaire's disease in humans. It enters host cells through phagocytosis and affects host cellular processes by secreting over 300 effector proteins. RavA, a L. pneumophila effector, displays unique structural characteristics and localizes to the Golgi apparatus and plasma membrane in human cells.
Article
Multidisciplinary Sciences
Yunjia Shi, Hongtao Liu, Kelong Ma, Zhao-Qing Luo, Jiazhang Qiu
Summary: Legionella organisms are environmental bacteria that cause Legionnaires' disease. A study characterized an effector protein, RavZ, in L. longbeachae, the second leading cause of Legionella infections. RavZ inhibits autophagy signaling in host cells challenged with L. pneumophila and L. longbeachae, indicating functional redundancy between RavZ(LLO) and RavZ(LP). RavZ(LLO) also reduces cellular levels of polyubiquitinated and polyneddylated proteins. RavZ(LLO) plays a role in the regulation of polyubiquitinated species on the Legionella-containing vacuole during L. longbeachae infection.
Review
Immunology
Tshegofatso Ngwaga, Deepika Chauhan, Stephanie R. Shames
Summary: The article discusses the immune responses mediated by effector proteins in Gram-negative bacterial pathogens and the potential future of developing new drugs using effector proteins.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Tao-Tao Chen, Yanling Lin, Shijun Zhang, Shuxin Liu, Lei Song, Wenhong Zhong, Zhao-Qing Luo, Aidong Han
Summary: The intracellular bacterial pathogen Legionella pneumophila hijacks host vesicular transport factor p115 to localize its effector protein LegA15 in host lipid droplets (LDs) and causes fragmentation of host Golgi apparatus. LegA15 is an atypical GTPase that modulates host LD homeostasis through its GTPase activity.
Article
Immunology
Alix McCloskey, Kayla Perri, TaoTao Chen, Aidong Han, Zhao-Qing Luo
Summary: Legionella pneumophila uses effectors and metaeffectors to manipulate host processes and regulate its virulence through specific mechanisms such as protein modification and direct binding.
MICROBES AND INFECTION
(2021)
Article
Microbiology
Deepika Chauhan, Ashley M. Joseph, Stephanie R. Shames
Summary: This study discovered a new intrabacterial mechanism in L. pneumophila virulence, in which MesI plays a crucial role. When uncoupled from MesI, SidI becomes toxic to the bacteria and triggers rapid degradation in host cells. The translocation of MesI is dispensable for intracellular replication, indicating the importance of intrabacterial regulation of SidI in L. pneumophila virulence.
Article
Microbiology
Anne-Sophie Godeux, Agnese Lupo, Marisa Haenni, Simon Guette-Marquet, Gottfried Wilharm, Maria-Halima Laaberki, Xavier Charpentier
JOURNAL OF BACTERIOLOGY
(2018)
Article
Multidisciplinary Sciences
Isabelle Durieux, Christophe Ginevra, Laetitia Attaiech, Kevin Picq, Pierre-Alexandre Juan, Sophie Jarraud, Xavier Charpentier
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2019)
Article
Microbiology
Gabriel Carvalho, David Fouchet, Gonche Danesh, Anne-Sophie Godeux, Maria-Halima Laaberki, Dominique Pontier, Xavier Charpentier, Samuel Venner
Review
Biochemistry & Molecular Biology
Margaux Pillon, Patricia Doublet
Summary: This review illustrates how bacteria manipulate host cell myosins to adhere, internalize, evade defense mechanisms, promote intracellular replication, spread in tissues, exit host cells, and avoid phagocytosis. It highlights the diversity and sophistication of bacterial strategies targeting the actin cytoskeleton.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
C. Jaboulay, As Godeux, P. Doublet, A. Vianney
Summary: This review aims to summarize the latest updates on the regulation of biogenesis and activity of T4SSs in important bacterial pathogens, highlighting the complexity and limited understanding of signals and processes involved in triggering machine assembly and substrate transfer during infection, despite significant progress in structure-function analyses.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Felix Bornier, Eline Zas, Damien Potheret, Maria-Halima Laaberki, Benedicte Coupat-Goutaland, Xavier Charpentier
Summary: This study found that the natural environment is a rich source of diverse amoebae with broad-spectrum predatory activities against human pathogens, including highly virulent and antibiotic-resistant clinical isolates.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
J. Allombert, C. Jaboulay, C. Michard, C. Andrea, X. Charpentier, A. Vianney, P. Doublet
Summary: The Legionella pneumophila Icm/Dot T4SS can translocate a large number of effectors, and research has found that their delivery is controlled by specific timing to effectively manipulate host cells. The timed delivery of effectors depends not only on chaperone protein interactions but also involves cyclic-di-GMP signaling.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Microbiology
Anne-Sophie Godeux, Elin Svedholm, Samuel Barreto, Anais Potron, Samuel Venner, Xavier Charpentier, Maria-Halima Laaberki
Summary: Acinetobacter baumannii infection poses a major health threat with antibiotic resistance. Natural transformation is identified as the main mechanism for the transfer of antibiotic resistance genes between different bacteria.
Article
Immunology
Johann Guillemot, Christophe Ginevra, Camille Allam, Elisabeth Kay, Christophe Gilbert, Patricia Doublet, Sophie Jarraud, Annelise Chapalain
Summary: This study demonstrates that different strains of Legionella pneumophila can induce varied immune responses and cell death in human macrophages. ST1 strains induce a higher inflammatory response and cell death, while ST47 strains induce a lower inflammatory response and cell death. The study also found that the Paris ST1 reference strain elicits a stronger immune response compared to the Lorraine ST47 reference strain.
Article
Infectious Diseases
Jean-Baptiste Vuillemenot, Maxime Bour, Racha Beyrouthy, Richard Bonnet, Maria-Halima Laaberki, Xavier Charpentier, Raymond Ruimy, Patrick Plesiat, Anais Potron
Summary: This study reveals the propensity of CC78 isolates to acquire multiple antibiotic resistance genes and transfer them to other strains via natural transformation.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2022)
Article
Multidisciplinary Sciences
Hyeong Jin Kim, Mazzen Black, Ross A. Edwards, Flora Peillard-Fiorente, Rashmi Panigrahi, David Klinger, Reiner Eidelpes, Ricarda Zeindl, Shiyun Peng, Jikun Su, Ayat R. Omar, Andrew M. MacMillan, Christoph Kreutz, Martin Tollinger, Xavier Charpentier, Laetitia Attaiech, J. N. Mark Glover
Summary: This study investigates the structural basis for RNA recognition by ProQ/FinO proteins and the importance of this recognition in repressing the uptake of environmental DNA in L. pneumophila. The authors also discover that other ProQ/FinO domains recognize transcriptional terminators with different specificities for the length of the 3' ssRNA tail.
NATURE COMMUNICATIONS
(2022)
Article
Immunology
Camille Allam, William Mouton, Hugo Testaert, Christophe Ginevra, Noemie Fessy, Marine Ibranosyan, Ghislaine Descours, Laetitia Beraud, Johann Guillemot, Annelise Chapalain, Chloe Albert-Vega, Jean-Christophe Richard, Laurent Argaud, Arnaud Friggeri, Vanessa Labeye, Yvan Jamilloux, Nathalie Freymond, Fabienne Venet, Gerard Lina, Patricia Doublet, Florence Ader, Sophie Trouillet-Assant, Sophie Jarraud
Summary: The study revealed that severe LD patients have a hyper-inflammatory response, with overproduction of multiple cytokines, and immune dysfunction with lower secretion capacity for certain cytokines. However, IL-18 secretion is increased in these patients. Assessment of the immune response may help identify patients suitable for potential host-directed therapies.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Microbiology
Leo Hardy, Pierre-Alexandre Juan, Benedicte Coupat-Goutaland, Xavier Charpentier
Summary: Legionella pneumophila, a pathogenic bacterium causing severe pneumonia in humans, has environmental life traits crucial to its infectiousness. Transposon insertion sequencing (TIS) can be used to identify essential genes and genetic basis of life traits, though its application in L. pneumophila has been limited. Successful implementation of TIS in clinical isolates of L. pneumophila has provided insights into essential genes, potential drug targets, and genes involved in horizontal gene transfer. This represents a significant step towards understanding the genetic basis of this pathogenic species.
JOURNAL OF BACTERIOLOGY
(2021)
Article
Microbiology
Romain Brunel, Ghislaine Descours, Isabelle Durieux, Patricia Doublet, Sophie Jarraud, Xavier Charpentier
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2018)
Article
Microbiology
Anne-Sophie Godeux, Elfin Svedholm, Samuel Barreto, Anais Potron, Samuel Venner, Xavier Charpentier, Maria-Halima Laaberki
Summary: This study reports the detection of spontaneous recombination events in mixed bacterial populations, revealing that natural transformation is the main mechanism for genome recombination and horizontal gene transfer of antibiotic resistance genes in Acinetobacter baumannii.