Article
Microbiology
Wouter J. C. Feitz, Romy Bouwmeester, Thea J. A. M. van der Velden, Susan Goorden, Christoph Licht, Lambert P. J. W. van den Heuvel, Nicole C. A. J. van de Kar
Summary: This study suggests that inhibiting the synthesis of Gb3 could be a potential therapeutic target for protecting against (further) endothelial damage caused by Stx in the future.
Article
Cell Biology
Yohei Kume, Hayato Go, Ryo Maeda, Kazuhide Suyama, Tsutomu Mori, Yukihiko Kawasaki, Koichi Hashimoto, Mitsuaki Hosoya
Summary: In this study, a mouse model of hemolytic uremic syndrome (HUS) induced by LPS/Stx2 was used. The results showed that LPS/Stx2 administration led to decreased urinary osmolality and urinary potassium, suggesting distal nephron disorders. Microarray analysis identified differentially expressed genes at 6 h and 72 h after LPS/Stx2 administration, with activation of TNFR1/2, iNOS, and IL-6 signaling observed at both time points. Additionally, pathways associated with lipid metabolism were inhibited at 72 h only. The downregulated genes were mainly expressed in the distal nephrons.
PHYSIOLOGICAL GENOMICS
(2022)
Article
Pharmacology & Pharmacy
David Arenas-Mosquera, Alipio Pinto, Natacha Cerny, Clara Berdasco, Adriana Cangelosi, Patricia Andrea Geoghegan, Emilio Luis Malchiodi, Mauricio De Marzi, Jorge Goldstein
Summary: This study aims to investigate the pathogenesis of encephalopathy associated with hemolytic uremic syndrome. The findings suggest that toxins released by enterohemorrhagic E. coli infection can trigger neuroinflammatory response and alterations in the neurovascular unit, which are associated with neurological compromise and abnormal motor behavior.
Article
Food Science & Technology
Rebecca A. A. Bova, Andrew C. C. Lamont, Theodore J. J. Picou, Vincent B. B. Ho, Kristin H. H. Gilchrist, Angela R. R. Melton-Celsa
Summary: Shiga toxins can cross the intestinal barrier and target kidney endothelial cells, and Stx2a translocates more than Stx1a in both single-layer and three-layer cell models. Infection with different strains of STEC affected barrier function differently, with O157:H7 reducing function and O26:H11 allowing translocation without reducing function.
Article
Cell Biology
Jana Tomisch, Vincent Busse, Francesca Rosato, Olga N. Makshakova, Pavel Salavei, Anna-Sophia Kittel, Emilie Gillon, Levin Lataster, Anne Imberty, Ana Valeria Melendez, Winfried Roemer
Summary: The study demonstrates the selective targeting of Gb3-positive cancer cells using the StxB-scFv UCHT1 lectibody, which induces a killing effect of up to 80% on Gb3-overexpressing cancer cells in vitro. This highlights the potential of lectibodies and lectins in general for enhancing the efficacy of established cancer treatments through immunotherapeutic approaches.
Article
Cell & Tissue Engineering
Ryo Hotta, Ahmed Rahman, Sukhada Bhave, Rhian Stavely, Weikang Pan, Shriya Srinivasan, Geoffrey de Couto, Luis Rodriguez-Borlado, Richard Myers, Alan J. Burns, Allan M. Goldstein
Summary: This study demonstrates that transplanted enteric neuronal stem cells (ENSCs) can form effective neuromuscular connections and improve colonic motor function in a model of colonic dysmotility. Multiple injections of ENSCs led to an improved response, paving the way for optimized clinical trial design.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Food Science & Technology
Aurelie Cointe, Etienne Bizot, Sabine Delannoy, Patrick Fach, Philippe Bidet, Andre Birgy, Francois-Xavier Weill, Sophie Lefevre, Patricia Mariani-Kurkdjian, Stephane Bonacorsi
Summary: The study identified a new heteropathotype clone among ST301 strains, with two main groups in the general phylogeny. A group mainly composed of enteropathogenic E. coli and another mostly including EHEC. The O55:H9 clone was found to have low genetic diversity but possessed multiple virulence factors, highlighting the need for close monitoring, especially of the virulent O55:H9 clone.
Article
Microbiology
Lekshmi K. Edison, Indira T. Kudva, Subhashinie Kariyawasam
Summary: This study aims to uncover the molecular mechanisms of Shiga toxin-producing Escherichia coli (STEC) in adapting and infecting different cell environments in cattle and humans. The comparative analysis of gene expression profiles revealed distinct patterns of gene expression between STEC interactions with bovine and human cells, highlighting the potential role of some genes in host adaptation and tissue-specific pathogenicity. These findings provide new insights into the colonization and infection mechanisms of STEC, contributing to its survival in the bovine reservoir and causing disease in humans.
Article
Food Science & Technology
Zhanqiang Su, Ling Zhang, Honghu Sun, Ying Hu, Seamus Fanning, Pengcheng Du, Shenghui Cui, Li Bai
Summary: The study evaluated the prevalence of non-O157 STEC isolated from beef and dairy cattle in Xinjiang, China. Results showed high genetic diversity among the isolates, some of which have potential to cause human diseases.
FOODBORNE PATHOGENS AND DISEASE
(2021)
Article
Multidisciplinary Sciences
Pascal Hoffmann, Marion Burmester, Marion Langeheine, Ralph Brehm, Michael T. Empl, Bettina Seeger, Gerhard Breves
Summary: This study combines simultaneous measurement of physiological properties on a functional and structural level with epithelial responses to bacterial toxins. Using this model, physiological responses of the intestine to bacterial toxins can be investigated and characterized.
Article
Medicine, Research & Experimental
Wen-Bo Wu, Xiao-Fei Jiang, Ming-Quan Chen
Summary: This study evaluated the diagnostic efficacy of miR-1-3p and T-synthase mRNA in patients with severe acute pancreatitis (SAP) complicated by intestinal mucosal barrier dysfunction (SAP&IBD). The results showed that miR-1-3p was elevated in the SAP&IBD group, while T-synthase mRNA expression was diminished. The combination of miR-1-3p and T-synthase mRNA exhibited diagnostic efficiency for SAP&IBD patients and was independently associated with IBD in SAP patients.
KAOHSIUNG JOURNAL OF MEDICAL SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Lisa M. Russo, Nourtan F. Abdeltawab, Alison D. O'Brien, Malak Kotb, Angela R. Melton-Celsa
Article
Plant Sciences
Kristin M. O'Neill, Anne M. Schilthuis, Calvin A. Leiter, Kurt M. Neihaus, Nicole A. Judge, Edda Twiddy, Alison D. O'Brien, Wayne R. Curtis
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
(2015)
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Immunology
Mark A. Smith, Rebecca A. Weingarten, Lisa M. Russo, Christy L. Ventura, Alison D. O'Brien
INFECTION AND IMMUNITY
(2015)
Article
Biotechnology & Applied Microbiology
Angela R. Melton-Celsa, Alison D. O'Brien, Peter C. H. Feng
JOURNAL OF FOOD PROTECTION
(2015)
Article
Food Science & Technology
Joshua C. Bunger, Angela R. Melton-Celsa, Ernest L. Maynard, Alison D. O'Brien
Article
Immunology
Lisa M. Russo, Angela R. Melton-Celsa, Alison D. O'Brien
JOURNAL OF INFECTIOUS DISEASES
(2016)
Article
Pathology
Wusheng Yan, Joanna Shih, Jaime Rodriguez-Canales, Michael A. Tangrea, Audrey Player, Lixia Diao, Nan Hu, Alisa M. Goldstein, Jing Wang, Philip R. Taylor, Scott M. Lippman, Ignacio I. Wistuba, Michael R. Emmert-Buck, Heidi S. Erickson
AMERICAN JOURNAL OF PATHOLOGY
(2013)
Article
Immunology
Tamako A. Garcia, Christy L. Ventura, Mark A. Smith, D. Scott Merrell, Alison D. O'Brien
INFECTION AND IMMUNITY
(2013)
Article
Multidisciplinary Sciences
Madison Humerick, Jeffrey Hanson, Jaime Rodriguez-Canales, Daniel Lubelski, Omar M. Rashid, Yasmmyn D. Salinas, YiJun Shi, Todd Ponzio, Raymond Fields, Michael R. Emmert-Buck, Harold Gainer
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Multidisciplinary Sciences
Lisa M. Russo, Angela R. Melton-Celsa, Michael J. Smith, Alison D. O'Brien
Article
Oncology
Nanyue Chen, Seetharaman Balasenthil, Jacquelyn Reuther, Aileen Frayna, Ying Wang, Dawn S. Chandler, Lynne V. Abruzzo, Asif Rashid, Jaime Rodriguez, Guillermina Lozano, Yu Cao, Erica Lokken, Jinyun Chen, Marsha L. Frazier, Aysegul A. Sahin, Ignacio I. Wistuba, Subrata Sen, Steven T. Lott, Ann McNeill Killary
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Gastroenterology & Hepatology
Nadia Boisen, Angela R. Melton-Celsa, Flemming Scheutz, Alison D. O'Brien, James P. Nataro
Article
Microbiology
James B. Kaper, Alison D. O'Brien
MICROBIOLOGY SPECTRUM
(2014)
Article
Microbiology
Angela R. Melton-Celsa, Alison D. O'Brien
MICROBIOLOGY SPECTRUM
(2014)
Article
Gastroenterology & Hepatology
Steven D. Zumbrun, Angela R. Melton-Celsa, Alison D. O'Brien