Article
Microbiology
Caroline Mullineaux-Sanders, Zuzanna Kozik, Julia Sanchez-Garrido, Eve G. D. Hopkins, Jyoti S. Choudhary, Gad Frankel
Summary: This study used Citrobacter rodentium to simulate infection and investigated the temporal responses of colonic intestinal epithelial cells post infection. Although the tissue morphology recovered, signs of infection were still present 4 weeks after pathogen clearance.
Article
Microbiology
Md Kamrul Hasan, Samir El Qaidi, Philip R. Hardwidge
Summary: The study investigated whether the T3SS effector protease NleC is active within C. rodentium, demonstrating that NleC is enzymatically active within C. rodentium and can proteolytically cleave NF-κB p65 subunit of host cells.
Article
Oncology
Cheng Cui, Theresa Barberi, Rahul Suresh, Alan D. Friedman
Summary: This study found that neuroblastoma cells expressing high levels of MYCN grow slower in mice lacking the p50 subunit in macrophages and neutrophils, and that tumors in these mice have increased T-cell numbers. Depletion of these T-cell populations accelerates tumor growth. Anti-PD-1 T-cell checkpoint blockade and epigenetic inhibition further slow tumor growth. Additionally, adoptive transfer of immature myeloid cells lacking p50 also slows tumor growth, highlighting the potential for targeting myeloid NF-kappa B p50 as a strategy for cancer therapy.
MOLECULAR ONCOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Norman Zhu, Matthew Mealka, Shane Mitchel, Christy Milani, Lisa M. Acuna, Eric Rogers, Ashlee N. Lahana, Tom Huxford
Summary: The NF-kappa B p50 homodimer, initially viewed as inactive, is now recognized as a key driver of specific target gene expression. This study investigated the structure of the p50 homodimer on different kappa B DNA sequences and found its preference for binding 11-bp. The findings also revealed the ability of the p50 dimerization domain to contact DNA via distinct modes.
Review
Biochemistry & Molecular Biology
Julia Sanchez-Garrido, David Ruano-Gallego, Jyoti S. Choudhary, Gad Frankel
Summary: Recent studies have shown that T3SS effectors interact with each other in the host, revealing their interdependency and context-dependent essentiality, which is of great significance for understanding the pathogenesis of the pathogens.
TRENDS IN MICROBIOLOGY
(2022)
Article
Oncology
Yue Liu, Kai Fu, Eric M. Wier, Yifan Lei, Andrea Hodgson, Dongqing Xu, Xue Xia, Dandan Zheng, Hua Ding, Cynthia L. Sears, Jian Yang, Fengyi Wan
Summary: This study identified a novel genotoxin called UshA in attaching/effacing pathogens, which triggers DNA damage and initiates tumorigenic transformation during bacterial infections. Furthermore, it was found that UshA plays a critical role in accelerating colon tumorigenesis in mice. These findings highlight the importance of UshA in the development of colon cancer caused by bacterial infections.
Article
Biochemistry & Molecular Biology
Ruoxi W. Wang, Sonia Vigano, Uri Ben-David, Angelika Amon, Stefano Santaguida
Summary: The study suggests that non-cell-autonomous mechanisms in aneuploid cells predominantly mediate their clearance by NK cells, with NF-kappa B signaling upregulation playing a central role in triggering the immune response. While NF-kappa B upregulation correlates with the degree of aneuploidy in cancer cell lines, it is not sufficient to trigger NK cell-mediated clearance, indicating that additional mechanisms may be at play during cancer evolution to counteract NF-kappa B-mediated immunogenicity.
Review
Oncology
Yelena Kravtsova-Ivantsiv, Gilad Goldhirsh, Ciprian Tomuleasa, Eli Pikarsky, Aaron Ciechanover
Summary: Nuclear factor-kappa B (NF-kappa B) is a transcriptional regulator that plays important roles in cell cycle, immune response, and malignant transformation. The study found that the ubiquitin ligase KPC1 stimulates the generation of the active subunit p50 from the NF-kappa B precursor p105 through ubiquitination. Overexpression of p50 has a strong tumor suppressive effect and stimulates the expression of tumor suppressive genes. The immune system contributes to the tumor suppressive activity of p50 by promoting the expression of pro-inflammatory cytokines and inhibiting the expression of programmed cell death-ligand 1 (PDL1).
CANCER CELL INTERNATIONAL
(2023)
Article
Biotechnology & Applied Microbiology
Jun-Young Kim, Sun-Min Seo, Han-Woong Kim, Woo-Jong Lee, Yang-Kyu Choi
Summary: This study aimed to evaluate the therapeutic ability of a novel TLR5 agonist, KMRC011, on ulcerative colitis induced by Citrobacter rodentium and dextran sulfate sodium in a mouse model. KMRC011 was administered before or after the C. rodentium infection, and it showed protective effects against colitis by inhibiting NF-kappa B signaling.
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Britta Marko, Paulina Heurich, Patrick Thon, Frieda Zimmer, Lars Bergmann, Hartmuth Nowak, Katharina Rump, Bjorn Koos, Michael Adamzik, Matthias Unterberg, Tim Rahmel
Summary: The NF-kappa B1 promoter polymorphism (-94ins/delATTG) is functionally important and plays a significant role in inflammation and sepsis. This study found that individuals with the deletion allele of this polymorphism exhibited lower nuclear activity of subunit p50, leading to aggravated inflammation and mitochondrial dysfunction.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Yuta Hasegawa, Shinichi Asada
Summary: NF-kappa B is a transcriptional factor involved in inflammation regulation. This study revealed the interaction between NF-kappa B p65 and DNA-PKcs, providing insight into the dysregulation mechanism of NF-kappa B.
BIOCHEMISTRY AND BIOPHYSICS REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Matthew Biancalana, Eviatar Natan, Michael J. Lenardo, Alan R. Fersht
Summary: The study reveals that the p50 and p65 Rel subunits in the NF-kappa B complex exhibit different kinetic stability, with a strong preference for the formation of the p50/p65 heterodimer over homodimers. This suggests that cells can modulate NF-kappa B activity by fine-tuning the relative proportions of p50 and p65 proteins and their expression time.
Review
Genetics & Heredity
Nadine Hoevelmeyer, Marc Schmidt-Supprian, Caspar Ohnmacht
Summary: Regulatory T cells (Treg cells) play a crucial role in modulating immune responses and preventing pathological immune reactions. NF-kappa B transcription factors have essential functions in Treg cell development and function.
JOURNAL OF MOLECULAR MEDICINE-JMM
(2022)
Article
Infectious Diseases
Tanaporn Wangsanut, Katelynn R. Brann, Haley E. Adcox, Jason A. Carlyon
Summary: This study reveals that Scrub typhus stabilizes p105 to inhibit the canonical NF-kappa B pathway, shedding light on how it counters host immunity to establish infection.
PLOS NEGLECTED TROPICAL DISEASES
(2021)
Article
Cell Biology
Shu Yang, Alexander Magnutzki, Najwa Ouali Alami, Michael Lattke, Tabea Melissa Hein, Judith Stefanie Scheller, Carsten Kroeger, Franz Oswald, Deniz Yilmazer-Hanke, Thomas Wirth, Bernd Baumann
Summary: The activation of NF-kappa B in astrocytes triggers a distinct neuroinflammatory response, which reduces the size and number of amyloid plaques in the brain of AD mice. Astrocytes and microglia exhibit different gene expression profiles, with microglia showing enhanced plaque clearance capacity.
Article
Microbiology
Daniel M. Mrochen, Dorothee Grumann, Daniel Schulz, Janine Gumz, Patricia Truebe, Kathleen Pritchett-Corning, Sarah Johnson, Werner Nicklas, Petra Kirsch, Karine Martelet, Jens van den Brandt, Sabine Berg, Barbara M. Broeker, Siouxsie Wiles, Silva Holtfreter
INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY
(2018)
Article
Microbiology
L. E. Lamb, X. Zhi, F. Alam, M. Pyzio, C. L. Scudamore, S. Wiles, S. Sriskandan
Article
Microbiology
Brin M. Ryder, Sarah K. Sandford, Kate M. Manners, James P. Dalton, Siouxsie Wiles, Joanna R. Kirman
FRONTIERS IN MICROBIOLOGY
(2019)
Article
Medicine, Research & Experimental
Alan F. Merry, Derryn A. Gargiulo, Ian Bissett, David Cumin, Kerry English, Christopher Frampton, Richard Hamblin, Jacqueline Hannam, Matthew Moore, Papaarangi Reid, Sally Roberts, Elsa Taylor, Simon J. Mitchell, Ian Chapman, Tim Denison, Lara Hopley, Laura Jackson, Rachael Jones, Cornelius Kruger, Helen Lindsay, Andrew Love, Shay McGuinness, Paget Milsom, Jacob Munro, Guido Panduri, Rocco Pitto, Janie Sheridan, Navdeep Sidhu, Francois Stapelberg, Colin Sweetman, Jane Torrie, Stuart Walker, Christine Walsh, Tim Willcox, Siouxsie Wiles, Simon Young
Review
Biochemistry & Molecular Biology
Sutharsana Yathursan, Siouxsie Wiles, Hannah Read, Vijayalekshmi Sarojini
JOURNAL OF PEPTIDE SCIENCE
(2019)
Article
Biochemistry & Molecular Biology
Melissa M. Cadelis, Soeren Geese, Lauren Gris, Bevan S. Weir, Brent R. Copp, Siouxsie Wiles
Article
Microbiology
Priscila Dauros-Singorenko, Siouxsie Wiles, Simon Swift
FRONTIERS IN MICROBIOLOGY
(2020)
Review
Infectious Diseases
Melissa M. Cadelis, Brent R. Copp, Siouxsie Wiles
Article
Multidisciplinary Sciences
Lesley Gray, Sally B. Rose, James Stanley, Jane Zhang, Natasha Tassell-Matamua, Viliami Puloka, Amanda Kvalsvig, Siouxsie Wiles, Samantha A. Murton, David M. Johnston, Julia S. Becker, Carol MacDonald, Michael G. Baker
Summary: The majority of participants in New Zealand were supportive of public health measures implemented to promote physical distancing. While most people showed understanding and willingness to adhere to distancing requirements, around half reported difficulties practicing physical distancing in public due to reasons like being essential workers and challenges related to the behavior of others.
JOURNAL OF THE ROYAL SOCIETY OF NEW ZEALAND
(2021)
Article
Biochemistry & Molecular Biology
Melissa M. Cadelis, Soeren Geese, Benedict B. Uy, Daniel R. Mulholland, Shara J. van de Pas, Alex Grey, Bevan S. Weir, Brent R. Copp, Siouxsie Wiles
Summary: Antimicrobial bioassay-guided fractionation of the endophytic fungi Neofusicoccum australe resulted in the isolation of a new unsymmetrical naphthoquinone dimer, neofusnaphthoquinone B (1), along with four known natural products. The antimicrobial activity of all the compounds was investigated, with compound 1 showing moderate activity against methicillin-resistant Staphylococcus aureus (MRSA) at a minimum inhibitory concentration of 16 μg/mL.
Article
Biochemistry & Molecular Biology
Melissa M. Cadelis, Hugo Gordon, Alex Grey, Soeren Geese, Daniel R. Mulholland, Bevan S. Weir, Brent R. Copp, Siouxsie Wiles
Summary: Fungi are a valuable source of bioactive natural products, and a recent study on Neodidymelliopsis sp. led to the discovery of several new compounds, including a polyketide that is sensitive to environmental conditions. Additionally, it was confirmed that pachybasin is biosynthesized through the acetate polyketide pathway.
Article
Biochemistry & Molecular Biology
Melissa M. Cadelis, Natasha S. L. Nipper, Alex Grey, Soeren Geese, Shara J. van de Pas, Bevan S. Weir, Brent R. Copp, Siouxsie Wiles
Summary: Screening of fungi from the New Zealand International Collection of Microorganisms from Plants identified two strains of Penicillium with antimicrobial activity. Further investigation led to the discovery of five known polyketide metabolites and a novel derivative. One of the polyketide metabolites and one derivative showed inhibitory activity against Methicillin-resistant Staphylococcus aureus.
Article
Economics
Shaun C. Hendy, Siouxsie Wiles, Rachelle Binny, Michael J. Plank
Summary: This article questions Gibson's analysis and points out the problems in his research. He neglects the spatio-temporal heterogeneity in the spread of COVID-19 in the United States, selects arbitrary cross-sectional data, and chooses an instrument variable that may be correlated with the explanatory variable, introducing bias.
NEW ZEALAND ECONOMIC PAPERS
(2022)
Article
Biotechnology & Applied Microbiology
Shilpa Saseendran Nair, Vikash Anand, Karnika De Silva, Siouxsie Wiles, Simon Swift
Summary: This study aims to determine the antimicrobial potency and mechanism of a surface-anchored quaternary ammonium salt (SAQAS)-based biocide during in vitro wet and dry fomite assays. The biocide showed effective killing of Gram-positive and Gram-negative bacteria, but not endospores. The mechanism of killing involved membrane depolarization, increased ROS production, and membrane permeability, leading to cell death.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Article
Primary Health Care
Lesley Gray, Carol MacDonald, Natasha Tassell-Matamua, James Stanley, Amanda Kvalsvig, Jane Zhang, Samantha Murton, Siouxsie Wiles, Viliami Puloka, Julia Becker, David Johnston, Michael G. Baker
JOURNAL OF PRIMARY HEALTH CARE
(2020)