Article
Immunology
Yunlong Zhao, Christine Caron, Ya-Yuan Chan, Calvin K. Lee, Xiaozhen Xu, Jibin Zhang, Takeya Masubuchi, Chuan Wu, Jack D. Bui, Enfu Hui
Summary: In this study, it was discovered that B7 ligands on CD8+ T cells interact with CD28 in a cis configuration at membrane invaginations of the immunological synapse, which is driven by phosphoinositide-3-kinase (PI3K) and sorting-nexin-9 (SNX9) mediated membrane remodeling. Cis-B7:CD28 interactions activate CD28 signaling through protein kinase C theta (PKCq), promoting CD8+ T cell survival, migration, and cytokine production.
Article
Multidisciplinary Sciences
Koen Debackere, Lukas Marcelis, Sofie Demeyer, Marlies Vanden Bempt, Nicole Mentens, Olga Gielen, Kris Jacobs, Michael Broux, Gregor Verhoef, Lucienne Michaux, Carlos Graux, Iwona Wlodarska, Philippe Gaulard, Laurence de Leval, Thomas Tousseyn, Jan Cools, Daan Dierickx
Summary: This study identifies two fusion transcripts that activate T cell receptor complex signaling and confer therapeutic vulnerability in PTCL-NOS, contributing to the understanding of this poorly characterized subgroup of PTCL at the genetic level.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Yuqi Zhu, Zhengtao Jiang, Lin Liu, Xinyi Yang, Min Li, Yipeng Cheng, Jianqing Xu, Chunhua Yin, Huanzhang Zhu
Summary: This study found that a compound called Scopoletin can reactivate latent HIV-1 infection and revealed its underlying mechanism. Using a Jurkat T cell model of HIV-1 latency, it was shown that Scopoletin can activate latent infection similarly to Prostratin in a dose- and time-dependent manner. The study also found that Scopoletin-induced HIV-1 reactivation involves the nuclear factor kappa B (NF-?B) signaling pathway. Overall, the findings suggest that Scopoletin has the potential to reactivate latent HIV-1 without causing global T-cell activation, making it a promising treatment option for anti-HIV-1 latency strategies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Zheng Cao, Duo Xu, Jeffrey Harding, Wenting Chen, Xiangsheng Liu, Zi Wang, Lan Wang, Tong Qi, Shilin Chen, Xinheng Guo, Irvin S. Y. Chen, Jimin Guo, Yunfeng Lu, Jing Wen
Summary: Cancer immunotherapy is limited by tumor immunosuppression caused by excessive lactate production. This study presents a nanocapsule enzyme therapeutic approach based on lactate oxidase, which reduces lactate levels and releases immunostimulatory hydrogen peroxide, improving the efficacy of immune checkpoint blockade treatment.
SCIENCE TRANSLATIONAL MEDICINE
(2023)
Article
Cell Biology
Qiudong Yang, Wenhua Zhao, Yuyi Chen, Yue Chen, Jiali Shi, Ran Qin, Hua Wang, Ruixia Wang, Hua Yuan, Wen Sun
Summary: The study reveals that the RelA/miR-30a/NLRP3 signaling axis is involved in rheumatoid arthritis by regulating the NLRP3 inflammasome in macrophages.
CELL DEATH & DISEASE
(2021)
Article
Immunology
Mingya Yang, Lei Wang, Ming Ni, Brigitte Neuber, Sanmei Wang, Wenjie Gong, Tim Sauer, Maria-Luisa Schubert, Angela Hueckelhoven-Krauss, Ruixiang Xia, Jian Ge, Christian Kleist, Volker Eckstein, Leopold Sellner, Carsten Mueller-Tidow, Peter Dreger, Michael Schmitt, Anita Schmitt
Summary: The study found that COX inhibitors have a negative impact on CD19.CAR-T cells, potentially leading to intracellular apoptosis, limited activation and proliferation, and inhibited cytokine release. This suggests that caution should be exercised when using COX inhibitors in CAR-T cell therapy for B cell lymphoma.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Yixiao Luo, Siyu Pei, Jing Xu, Yichuan Xiao, Xiaodong Zhu
Summary: The two-drug combined chemotherapy of platinum and fluorouracil enhances the proliferation ability of T cells in patients with advanced gastric cancer, promotes T cell differentiation into specific subtypes, suppresses tumor growth, and activates T cell-mediated antitumor immunity. This study reveals a previously unidentified function of platinum and fluorouracil combination chemotherapy in enhancing T cell responses against tumor cells.
ACTA BIOCHIMICA ET BIOPHYSICA SINICA
(2021)
Article
Biotechnology & Applied Microbiology
Jakrawadee Julamanee, Seitaro Terakura, Koji Umemura, Yoshitaka Adachi, Kotaro Miyao, Shingo Okuno, Erina Takagi, Toshiyasu Sakai, Daisuke Koyama, Tatsunori Goto, Ryo Hanajiri, Michael Hudecek, Peter Steinberger, Judith Leitner, Tetsuya Nishida, Makoto Murata, Hitoshi Kiyoi
Summary: CD19.79a.40z CAR-T cells showed enhanced activity in vitro and superior anti-tumor efficacy in a murine model, with increased proliferative capacity compared to CD19.28z and CD19.BBz CAR-T cells.
Article
Virology
Jayeshbhai Chaudhari, Chia-Sin Liew, Jean-Jack M. Riethoven, Sarah Sillman, Hiep L. X. Vu
Summary: By comparing the host transcriptional response between PRRSV-infected PAMs and uninfected but exposed bystander PAMs, it was found that inflammatory cytokines, interferon-stimulated genes, and antiviral genes were highly upregulated in infected cells. Additionally, negative immune regulators and T-cell exhaustion markers were also significantly upregulated in infected PAMs compared to bystander cells.
JOURNAL OF VIROLOGY
(2021)
Article
Nanoscience & Nanotechnology
Xinghan Liu, Yujun Xu, Lijie Yin, Yayi Hou, Shuli Zhao
Summary: The study demonstrates that R-M@CS-PAA NPs can exert anti-tumor effects by promoting the infiltration of M1 macrophages and CD8(+) T cells, reducing the number of suppressive immune cells in the tumor microenvironment.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2021)
Article
Hematology
Fengjie Liu, Yumei Gao, Bufang Xu, Shan Xiong, Shengguo Yi, Jingru Sun, Zhuojing Chen, Xiangjun Liu, Yingyi Li, Yuchieh Lin, Yujie Wen, Yao Qin, Shuxia Yang, Hang Li, Trilokraj Tejasvi, Lam Tsoi, Ping Tu, Xianwen Ren, Yang Wang
Summary: This study identified unique transcriptional programs and enriched expression of genes at the chr7q locus in tumors undergoing large-cell transformation (LCT) in late-stage mycosis fungoides (MF). Aberrant expression of the imprinted gene Paternally Expressed Gene 10 (PEG10) in LCT was shown to drive cell size increase, promote proliferation, and confer treatment resistance through a PEG10/KLF2/NF-kappa B axis. Pharmacological targeting of PEG10 reversed the proliferation and treatment resistance phenotypes in LCT, suggesting PEG10 inhibition as a promising therapeutic approach.
Article
Genetics & Heredity
Ninghan Zhang, Jiawen Xu, Rong Wang, Ting Pan, Huanxin Zhang, Lingling Yin, Yao Yao, Linyan Xu, Shengyun Zhu, Qingyun Wu, Zhenyu Li, Xuejiao Liu, Kailin Xu, Mingshan Niu
Summary: The study demonstrates that CARMA1 is highly expressed in T-ALL cells and correlated with prognosis. Knockdown of CARMA1 only impacts the growth and proliferation of SIL-TAL1 fusion gene-negative T-ALL cells.
JOURNAL OF MOLECULAR MEDICINE-JMM
(2021)
Article
Biology
Elliott D. SoRelle, Joanne Dai, Emmanuela N. Bonglack, Emma M. Heckenberg, Jeffrey Y. Zhou, Stephanie N. Giamberardino, Jeffrey A. Bailey, Simon G. Gregory, Cliburn Chan, Micah A. Luftig
Summary: The study characterized single-cell transcriptomic profiles of five LCLs, revealing substantial phenotypic heterogeneity within and across LCLs likely due to intrinsic variance in primary B cells and host-pathogen dynamics. Stochastic simulations demonstrate that factors such as initial primary cell heterogeneity, random sampling, time in culture, and phenotype-specific fitness differences can contribute substantially to dynamic diversity in populations of nominally clonal cells.
Article
Multidisciplinary Sciences
Tingting Yuan, Honglin Tang, Xiaojie Xu, Jingjing Shao, Gaojun Wu, Young-Chang Cho, Yuan Ping, Guang Liang
Summary: This study developed an inflammation-inducible CRISPR-Cas9 system by grafting a sequence that binds with NF-KB to the CRISPR-Cas9 framework, named NBS-CRISPR. By targeting the MyD88 gene, a crucial player in the NF-KB signaling pathway, this system successfully reversed inflammatory conditions and achieved impressive therapeutic effects.
Article
Medicine, Research & Experimental
Jun Xiao, Fei Sun, Ya-Nan Wang, Bo Liu, Peng Zhou, Fa-Xi Wang, Hai-Feng Zhou, Yue Ge, Tian-Tian Yue, Jia-Hui Luo, Chun-Liang Yang, Shan-Jie Rong, Ze-Zhong Xiong, Sheng Ma, Qi Zhang, Yang Xun, Chun-Guang Yang, Yang Luan, Shao-Gang Wang, Cong-Yi Wang, Zhi-Hua Wang
Summary: The role of tumor-associated macrophages (TAMs) and the regulatory mechanisms of macrophage activation in prostate cancer (PCa) are poorly understood. In this study, it was found that PCa growth in mice with macrophage-specific Ubc9 deficiency was suppressed due to enhanced CD8+ T cell response. The study also identified STAT4 as a crucial UBC9-mediated SUMOylation target, with K350 as the major modification site. Furthermore, inhibition of UBC9 with the inhibitor 2-D08 increased the antitumor effect of TAMs and expression of PD-1 on CD8+ T cells, providing valuable insights for cancer therapy.
JOURNAL OF CLINICAL INVESTIGATION
(2023)