Review
Cell Biology
Jose Luis Rodriguez-Fernandez, Olga Criado-Garcia
Summary: Dendritic cells are powerful antigen-presenting cells that control T cell activation through the immunological synapse. While most studies focus on the T cell side, dendritic cell side also plays a significant role in T cell activation, especially through actin polarization at the IS.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Noriko Sato, Richard N. Bamford, Bonita R. Bryant, Yutaka Tagaya, Thomas A. Waldmann
Summary: When purified, naive T cells and regulatory T cells could not proliferate to the γC-cytokines IL-2, IL-7, or IL-15, despite expressing the cognate cytokine receptors. However, dendritic cells enabled their proliferation through cell-to-cell contact, independent of T cell receptor stimulation. This preconditioning effect activated specific cellular pathways and facilitated cytokine-mediated proliferation of T cells.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Review
Immunology
Diego Calzada-Fraile, Francisco Sanchez-Madrid
Summary: Dendritic cells (DCs) play a crucial role in innate and adaptive immunity by presenting antigens to activate T cells. This review focuses on the effects of antigen presentation on DCs and discusses the potential therapeutic applications of reverse information transfer during immune synapse (IS) formation.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2023)
Article
Immunology
Eleonora Gallerani, Davide Proietto, Beatrice Dallan, Marco Campagnaro, Salvatore Pacifico, Valentina Albanese, Erika Marzola, Peggy Marconi, Antonella Caputo, Victor Appay, Riccardo Gavioli, Francesco Nicoli
Summary: Research indicates that older individuals have defective primary SARS-CoV-2-specific CD8(+) T-cell responses, with lower magnitude and quality compared to younger adults, as well as recognizing a lower number of epitopes.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Immunology
Izumi Sasaki, Takashi Kato, Hiroaki Hemmi, Yuri Fukuda-Ohta, Naoko Wakaki-Nishiyama, Asumi Yamamoto, Tsuneyasu Kaisho
Summary: Dendritic cells (DC) are essential in connecting innate and adaptive immunity. Among the subsets, cDC1 plays a critical role in maintaining intestinal immune homeostasis.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Durga Reddi, Lydia Durant, David Bernardo, Alistair Noble, Nicholas R. English, Philip Hendy, Graeme C. Clark, Joann L. Prior, Ethel Diane Williamson, Stella C. Knight
Summary: The study demonstrated the in vitro priming of naïve human T cells against heat-killed B. pseudomallei by co-culturing with syngeneic, pulsed dendritic cells. Myeloid DCs showed a major response to B. pseudomallei, contributing significantly to the expression of migration marker CCR7 and secretion of proinflammatory cytokines TNF alpha and IL1 beta. The study suggests that this approach could be used to screen for human cellular response to candidate vaccines and enhance cell-mediated immunity against intracellular pathogens.
Article
Immunology
Florianne M. J. Hafkamp, Esther W. M. Taanman-Kueter, Toni M. M. van Capel, Tom Groot Kormelink, Esther C. de Jong
Summary: This study investigated the effects of Vitamin D3 on the differentiation of specific human T cells. The results showed that Vitamin D3 can restrict the development of Th17 cells, promote the development of regulatory T cells, and reduce the production of specific cytokines by dendritic cells. This provides potential for the use of Vitamin D3 as an adjuvant in the treatment of autoimmune disorders.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Virology
Nozomi Kuse, Tomohiro Akahoshi, Masafumi Takiguchi
Summary: The study demonstrated that priming with STING ligand 3'3'-cGAMP effectively activated HLA-B*52:01-restricted CD8(+) T cells, which showed strong ability to suppress HIV-1 replication and expressed high levels of cytolytic effector molecules. This suggests that priming with STING ligand of functional CD8(+) T cells specific for protective epitopes could be beneficial in an HIV-1 cure therapy.
JOURNAL OF VIROLOGY
(2021)
Review
Immunology
Naoya Tatsumi, Yosuke Kumamoto
Summary: Conventional dendritic cells (cDCs) are a powerful type of antigen-presenting cells, consisting of different subsets with distinct developmental, phenotypic, and functional characteristics. After immunization, each subset of cDCs acquires antigens in the secondary lymphoid organs and presents them to CD4T cells in a spatiotemporal manner, resulting in multiple waves of antigen presentation. This review focuses on the kinetics of antigen presentation by each cDC subset and its impact on priming naive CD4T cells, as well as its implications in CD4T cell differentiation.
CURRENT OPINION IN IMMUNOLOGY
(2023)
Article
Multidisciplinary Sciences
Ana Alcaraz-Serna, Eugenio Bustos-Moran, Irene Fernandez-Delgado, Diego Calzada-Fraile, Daniel Torralba, Ester Marina-Zarate, Erika Lorenzo-Vivas, Enrique Vazquez, Juliana Barreto de Alburquerque, Nora Ruef, Manuel Jose Gomez, Fatima Sanchez-Cabo, Ana Dopazo, Jens Stein, Almudena Ramiro, Francisco Sanchez-Madrid
Summary: Researchers have discovered that dendritic cells switch their transcriptomic signature, epigenomic changes, and enhanced migratory capacity after forming productive immune synapses with T cells.
Article
Immunology
Bilade Cherqaoui, Frederic Cremazy, Marc Lauraine, Ghazal Shammas, Roula Said-Nahal, Hendrick Mambu Mambueni, Felicie Costantino, Marine Fourmont, Audrey Hulot, Henri-Jean Garchon, Simon Glatigny, Luiza M. Araujo, Maxime Breban
Summary: The naïve CD4+ T cells of B27-rats exhibit a predisposition towards Th17 differentiation due to a STAT1 deficiency, which occurs early and is associated with an epigenetic Th17 bias. This molecular phenomenon may contribute to the persistent proinflammatory skew of CD4+ T cells in SpA patients, providing new insights for understanding and treating SpA.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Immunology
Bonah Kim, Hee Young Kim, Won-Woo Lee
Summary: Zinc, an essential microelement, plays immunoregulatory roles by influencing signaling pathways to enhance immune function. Zinc deficiency impairs immunity and increases susceptibility to infection. The interaction between Zip6-mediated zinc influx and TCR activation regulates T-cell responses.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Cell Biology
Youhui Si, Yihan Wang, Qiaomu Tian, Qiang Wang, Jared M. Pollard, Pramod K. Srivastava, Aaron P. Esser-Kahn, Joel H. Collier, Anne I. Sperling, Anita S. Chong
Summary: The current study provides evidence that intranasal administration of peptide antigens primes naive CD8+ T cells in the lung independently before priming in the draining mediastinal lymph node. The transcriptional responses of lung cDC1 and cDC2 to intranasal nanofiber immunization were found to be distinct, but both subsets acquire shared transcriptional responses upon migration into the lymph node, revealing a stepwise activation process of cDC1 and cDC2 towards their ability to cross-prime effector and functional memory CD8+ T cell responses.
Article
Biochemistry & Molecular Biology
Faezzah Baharom, Ramiro A. Ramirez-Valdez, Ahad Khalilnezhad, Shabnam Khalilnezhad, Marlon Dillon, Dalton Hermans, Sloane Fussell, Kennedy K. S. Tobin, Charles-Antoine Dutertre, Geoffrey M. Lynn, Soren Muller, Florent Ginhoux, Andrew S. Ishizuka, Robert A. Seder
Summary: Therapeutic cancer vaccines aim to enhance tumor-specific T cell immunity, but suppressive mechanisms within the tumor microenvironment can limit T cell function. This study investigated how different routes of vaccination impact intratumoral myeloid cells and found that intravenous administration of a nanoparticle vaccine induced tumor regression mediated by systemic type I interferon, leading to a reduction in intratumoral monocytes expressing immune-regulatory genes. In humans, these gene signatures are associated with worse outcomes. These results suggest that combining the generation of tumor-specific CD8+ T cells with remodeling of the tumor microenvironment holds promise for tumor immunotherapy.
Review
Cell Biology
Huiping Shi, Bojing Shao
Summary: This review briefly summarizes the activation and roles of LFA-1 with varied ligand-binding affinities in the regulation of T-cell migration and immunological synapse formation.
Article
Immunology
Benjamin Diethelm-Varela, Antonia Reyes, Yvonne Rosenstein, Jorge Kalil, Marcelo Hill, Guillermo Docena, Ignacio Anegon, Pablo A. Gonzalez, Alexis M. Kalergis
Summary: FOCIS Goes South is a course and workshop organized by FOCIS Centers of Excellence (FCEs) from across Latin America, focusing on advanced immunology, flow cytometry, as well as cutting-edge research in autoimmunity, tolerance, cancer, infectious diseases, and vaccines. The second edition of FOCIS Goes South took place virtually with over 950 registered participants due to the COVID-19 pandemic.
Review
Biochemistry & Molecular Biology
Enrique Gonzalez-Madrid, Ma. Andreina Rangel-Ramirez, Maria Jose Mendoza-Leon, Oscar Alvarez-Mardones, Pablo A. Gonzalez, Alexis M. Kalergis, Ma. Cecilia Opazo, Claudia A. Riedel
Summary: This review discusses the relevance of the gestational period for the development of multiple sclerosis (MS) in adulthood, pointing out that thyroid hormone deficiency during pregnancy may increase susceptibility to MS.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Barbara M. Schultz, Felipe Melo-Gonzalez, Luisa F. Duarte, Nicolas M. S. Galvez, Gaspar A. Pacheco, Jorge A. Soto, Roslye Berrios-Rojas, Liliana A. Gonzalez, Daniela Moreno-Tapia, Daniela Rivera-Perez, Mariana Rios, Yaneisi Vazquez, Guillermo Hoppe-Elsholz, Catalina A. Andrade-Parra, Omar P. Vallejos, Alejandro Pina-Iturbe, Carolina Iturriaga, Marcela Urzua, Maria S. Navarrete, Alvaro Rojas, Rodrigo Fasce, Jorge Fernandez, Judith Mora, Eugenio Ramirez, Aracelly Gaete-Argel, Monica Acevedo, Fernando Valiente-Echeverria, Ricardo Soto-Rifo, Daniela Weiskopf, Alba Grifoni, Alessandro Sette, Gang Zeng, Weining Meng, Jose Gonzalez-Aramundiz, Pablo A. Gonzalez, Katia Abarca, Alexis M. Kalergis, Susan M. Bueno
Summary: This study reports the impact of a booster dose of CoronaVac on the immune response against Delta and Omicron variants. Four weeks after the booster dose, there was a significant increase in neutralizing antibodies, and specific T cells reached their peak. Furthermore, the immune response induced by the booster showed activity against Delta and Omicron variants.
Article
Multidisciplinary Sciences
Pablo F. Cespedes, Ashwin Jainarayanan, Lola Fernandez-Messina, Salvatore Valvo, David G. Saliba, Elke Kurz, Audun Kvalvaag, Lina Chen, Charity Ganskow, Huw Colin-York, Marco Fritzsche, Yanchun Peng, Tao Dong, Errin Johnson, Jesus A. Siller-Farfan, Omer Dushek, Erdinc Sezgin, Ben Peacock, Alice Law, Dimitri Aubert, Simon Engledow, Moustafa Attar, Svenja Hester, Roman Fischer, Francisco Sanchez-Madrid, Michael L. Dustin
Summary: The authors use bead-supported lipid bilayers as synthetic APCs to show that trans-synaptic vesicles produced by T cells in the immunological synapse carry specialized cargos distinct from constitutive extracellular vesicles to serve as intercellular messengers.
NATURE COMMUNICATIONS
(2022)
Review
Cell Biology
Monica A. Farias, Benjamin Diethelm-Varela, Areli J. Navarro, Alexis M. Kalergis, Pablo A. Gonzalez
Summary: This article reviews and discusses the interplay between neutral lipid metabolism and lipid droplets (LDs) in the replication cycle of different DNA viruses. It highlights the role of LDs in modulating viral infection and identifies potentially new molecular targets for the treatment of viral infections.
Article
Immunology
Katia Abarca, Carolina Iturriaga, Marcela Urzua, Nicole Le Corre, Augusto Pineda, Carolina Fernandez, Angelica Dominguez, Pablo A. Gonzalez, Susan M. Bueno, Paulina Donato, Pilar Espinoza, Daniela Fuentes, Marcela Gonzalez, Paula Guzman, Paula Munoz-Venturelli, Carlos M. Perez, Marcela Potin, Alvaro Rojas, Jose Gonzalez-Aramundiz, Nicolas M. S. Galvez, Francisca Aguirre-Boza, Sofia Aljaro, Luis Federico Batiz, Yessica Campisto, Mariela Cepeda, Aaron Cortes, Sofia Lopez, Maria Loreto Perez, Andrea Schilling, Alexis M. Kalergis
Summary: This study investigated the safety and effectiveness of two different immunization schedules of CoronaVac(R) in healthy adults. The results showed that CoronaVac(R) vaccine is safe and effective in preventing severe cases of COVID-19. There were no significant differences in adverse events between the two schedules, and both schedules provided protection against COVID-19 hospitalization.
Article
Public, Environmental & Occupational Health
Humberto Reyes, Benjamin Diethelm-Varela, Constanza Mendez, Diego Rebolledo-Zelada, Bastian Lillo-Dapremont, Sergio R. Munoz, Susan M. Bueno, Pablo A. Gonzalez, Alexis M. Kalergis
Summary: This study evaluates the impact of Chile's initial two-dose vaccination campaign in reducing adverse epidemiological outcomes due to COVID-19. The results suggest that completing vaccination schedules is crucial for minimizing negative outcomes during the pandemic.
FRONTIERS IN PUBLIC HEALTH
(2022)
Article
Biology
Nicolas M. S. Galvez, Gaspar A. Pacheco, Barbara M. Schultz, Felipe Melo-Gonzalez, Jorge A. Soto, Luisa F. Duarte, Liliana A. Gonzalez, Daniela Rivera-Perez, Mariana Rios, Roslye Berrios, Yaneisi Vazquez, Daniela Moreno-Tapia, Omar P. Vallejos, Catalina A. Andrade, Guillermo Hoppe-Elsholz, Carolina Iturriaga, Marcela Urzua, Maria S. Navarrete, Alvaro Rojas, Rodrigo Fasce, Jorge Fernandez, Judith Mora, Eugenio Ramirez, Aracelly Gaete-Argel, Monica L. Acevedo, Fernando Valiente-Echeverria, Ricardo Soto-Rifo, Daniela Weiskopf, Alba Grifoni, Alessandro Sette, Gang Zeng, Weining Meng, Jose Gonzalez-Aramundiz, Marina Johnson, David Goldblatt, Pablo A. Gonzalez, Katia Abarca, Susan M. Bueno, Alexis M. Kalergis
Summary: This study found that immunization with CoronaVac in Chilean adults promotes robust cellular and humoral immune responses. The 0-28 schedule induced a stronger humoral immune response than the 0-14 schedule.
Review
Immunology
Pedro H. Silva, Yaneisi Vazquez, Camilo Campusano, Angello Retamal-Diaz, Margarita K. Lay, Christian A. Munoz, Pablo A. Gonzalez, Alexis M. Kalergis, Susan M. Bueno
Summary: Streptococcus pneumoniae is a major cause of pneumonia in children and the elderly worldwide. While there are currently vaccines available, there is a need for non-capsular-based vaccines to overcome certain limitations.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Review
Virology
Linmar Rodriguez-Guilarte, Mario A. Ramirez, Catalina A. Andrade, Alexis M. Kalergis
Summary: LAG-3 is an inhibitory receptor expressed on immune cells, which regulates T cell activation and effector functions in the immune response. Immunotherapies targeting immune checkpoints, including LAG-3, have shown promise in controlling malignant tumors and chronic viral diseases.
Review
Microbiology
Monica A. Farias, Benjamin Diethelm-Varela, Alexis M. Kalergis, Pablo A. Gonzalez
Summary: Lipids play crucial roles in the cell as membrane components, signaling molecules, and energy storage sources. Lipid droplets, composed of neutral lipids such as triglycerides and cholesterol esters, are not only cellular energy reserves but also involved in regulating oxidative stress, cellular lipotoxicity, and modulating pathogen infection through immune regulation. Lipid metabolism and lipid droplets play important roles in the replication cycle of RNA viruses, and understanding these processes may reveal new targets for antiviral drugs.
CRITICAL REVIEWS IN MICROBIOLOGY
(2023)
Article
Hematology
Yolanda Prado, Lorena Perez, Felipe Eltit, Cesar Echeverria, Felipe M. Llancalahuen, Pablo Tapia, Pablo A. Gonzalez, Alexis M. Kalergis, Claudio Cabello-Verrugio, Felipe Simon
Summary: Severe systemic inflammation can lead to oxidative stress, promoting the conversion of high-density lipoprotein (HDL) to oxidized HDL (oxHDL), which interacts with vascular endothelial cells (ECs). OxHDL can acquire procoagulant features and modulate coagulation, which has been linked with organ failure in ICU patients. However, the exact role and underlying molecular mechanism of oxHDL in generating a procoagulant phenotype and causing organ failure and death are still unknown.
THROMBOSIS RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Eduardo I. Tognarelli, Luisa F. Duarte, Monica A. Farias, Felipe A. Cancino, Nicolas Corrales, Francisco J. Ibanez, Claudia A. Riedel, Susan M. Bueno, Alexis M. Kalergis, Pablo A. Gonzalez
Summary: Stimulating HO-1 expression in HSV-infected dendritic cells can promote cell viability and inhibit viral egress. It also promotes the expression of anti-inflammatory molecules and activation of virus-specific CD4(+) T cells. Transferring HSV-infected dendritic cells with induced HO-1 expression can enhance virus-specific T cell activation and improve the outcome of HSV-1 skin infection.
Article
Immunology
Sebastian Mondaca, Benjamin Walbaum, Nicole Le Corre, Marcela Ferres, Alejandro Valdes, Constanza Martinez-Valdebenito, Cinthya Ruiz-Tagle, Patricia Macanas-Pirard, Patricio Ross, Betzabe Cisternas, Patricia Perez, Olivia Cabrera, Valentina Cerda, Ivana Ormazabal, Aldo Barrera, Maria E. Prado, Maria I. Venegas, Silvia Palma, Richard Broekhuizen, Alexis M. Kalergis, Susan M. Bueno, Manuel A. Espinoza, M. Elvira Balcells, Bruno Nervi
Summary: Cancer patients receiving chemotherapy treatment have a lower immune response to SARS-CoV-2 vaccines. Our study demonstrated that booster vaccines elicit strong humoral and cellular responses among cancer patients receiving chemotherapy, regardless of the type of vaccine used as a priming dose. The homologous vaccine regimen was associated with higher neutralizing antibody positivity and total antibody levels.
Review
Microbiology
Xanthippi Topalidou, Alexis M. Kalergis, Georgios Papazisis
Summary: RSV is a significant cause of morbidity and mortality in young children and older adults. The development of RSV vaccines is guided by epidemiologic and pathophysiological evidence, using various vaccine technologies. Currently, there are 24 vaccines in clinical development, including two licensed vaccines.