Article
Biotechnology & Applied Microbiology
Jiafeng Xia, Shiman Jiang, Longxian Lv, Wenrui Wu, Qiangqiang Wang, Qiaomai Xu, Jianzhong Ye, Daiqiong Fang, Yating Li, Jingjing Wu, Xiaoyuan Bian, Liya Yang, Huiyong Jiang, Kaicen Wang, Ren Yan, Lanjuan Li
Summary: Lactobacillus salivarius LI01 can directly impact the host by modulating immunity and metabolism, including restoring levels of serum biomarkers, activating Th0 cell differentiation, stimulating immune response and metabolic processes, among other effects.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Gastroenterology & Hepatology
Ran Wang, Md Moniruzzaman, Kuan Yau Wong, Percival Wiid, Alexa Harding, Rabina Giri, Wendy (Hui) Tong, Jackie Creagh, Jakob Begun, Michael A. McGuckin, Sumaira Z. Hasnain
Summary: The study found that microbiota plays a role in the development of Winnie colitis but is not essential, as protein misfolding can directly trigger inflammation in the absence of microbiota.
Article
Microbiology
Xin Shen, Hong Wei, Jian Li, Wei Wei, Bo Zhang, Changqing Lu, Caixia Yan, Shuzhen Li, Lirong Bao, Jinmei Zhang, Cheng Zhang, Yan Li
Summary: The relationship between diabetes and periodontitis has been explored recently. This study investigated the immune regulatory effects of periodontitis microbiota on diabetes and the correlation between immunomodulation and ectopic colonization. The findings suggest that periodontitis microbiota can invade extra-oral sites and cause immune cells infiltration, leading to local or systemic inflammation which may worsen diabetes.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Xin Shen, Yutao Yang, Jian Li, Bo Zhang, Wei Wei, Changqing Lu, Caixia Yan, Hong Wei, Yan Li
Summary: This study provides an immunoregulation map of key periodontal bacteria, showing that P. gingivalis induces the strongest innate immune response in gingiva, F. nucleatum widely induces immune dysregulation in the colon, and T. denticola mainly induces immune disorder in the spleen.
Article
Genetics & Heredity
Yanze Li, Wenming Cao, Na L. Gao, Xing-Ming Zhao, Wei-Hua Chen
Summary: This study aimed to identify human gut microbes that undergo significant and consistent changes after transplantation into germ-free mice. The results showed that only about 47% of the human gut microbes could be re-established at the species level in mice, and more than 1/3 of them underwent significant changes. Most of the microbes that underwent significant changes were consistent across multiple experimental settings, providing important information for researchers using mice in gut microbiota studies.
GENOMICS PROTEOMICS & BIOINFORMATICS
(2022)
Article
Physiology
Chiara H. Moretti, Tomas A. Schiffer, Xuechen Li, Eddie Weitzberg, Mattias Carlstrom, Jon O. Lundberg
Summary: Studies in the past 15 years have shown the significant role of the gut microbiota in host metabolism. However, this study found that both germ-free and conventionally raised mice gained weight and developed metabolic dysfunction when fed a Western diet. The microbiota is not considered an essential factor in the onset of obesity and metabolic disorders according to the results of this study, suggesting that further exploration is needed in understanding the relationship between gut bacteria and metabolic diseases.
Article
Geriatrics & Gerontology
Kristina S. Fluitman, Mark Davids, Louise E. Olofsson, Madelief Wijdeveld, Valentina Tremaroli, Bart J. F. Keijser, Marjolein Visser, Fredrik Backhed, Max Nieuwdorp, Richard G. IJzerman
Summary: This study found associations between the gut microbiota and both poor appetite and undernutrition in older adults. Additionally, by transferring fecal microbiota from older adults to germ-free mice, the study suggests a potential causal relationship between the aged gut microbiota and body weight and composition. These findings indicate that microbiota-manipulating strategies may benefit older adults prone to undernutrition.
JOURNAL OF CACHEXIA SARCOPENIA AND MUSCLE
(2022)
Article
Nutrition & Dietetics
Marijke M. Faas, Yuanrui Liu, Lieske Wekema, Gisela A. Weiss, Carolien A. van Loo-Bouwman, Luis Silva Lagos
Summary: This study found that inducing gut dysbiosis during pregnancy by antibiotic treatment alters the maternal immune response, which may affect fetal and placental weight.
Article
Food Science & Technology
Meiling Liu, Qingyi Li, Na Wang, Yuye Liu, Lili Wang, Jiurui Wang, Mengjun Liu, Zhihui Zhao
Summary: This study found that acetylated jujube polysaccharide (Ac-JP) enhanced the immune response and altered the composition of intestinal microbiota in immunosuppressed mice. These results suggest that Ac-JP has the potential to be a promising immunomodulator.
JOURNAL OF FUNCTIONAL FOODS
(2023)
Article
Gastroenterology & Hepatology
Elaine M. Glenny, Farnaz Fouladi, Stephanie A. Thomas, Emily C. Bulik-Sullivan, Quyen Tang, Zorka Djukic, Yesel S. Trillo-Ordonez, Anthony A. Fodor, Lisa M. Tarantino, Cynthia M. Bulik, Ian M. Carroll
Summary: Analyses show a significant association between gut microbiota from patients with anorexia nervosa and body weight, fat mass, and cecum weight in colonized germ-free mice, regardless of the donor group, suggesting a strong impact of human fecal microbes on these physiological parameters.
Article
Immunology
Yapeng Yang, Jinhui He, Yuqing Wang, Lifeng Liang, Zeyue Zhang, Xiang Tan, Shiyu Tao, Zhifeng Wu, Miaomiao Dong, Jixia Zheng, Hang Zhang, Shuaifei Feng, Wei Cheng, Qiyi Chen, Hong Wei
Summary: Whole intestinal microbiota transplantation (WIMT) is more effective than fecal microbiota transplantation (FMT) in the treatment of inflammatory bowel disease (IBD). WIMT can replicate the community structure more accurately and reduce the inflammatory response of the host, alleviating the symptoms of IBD.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Multidisciplinary Sciences
Jocelyn M. Choo, Geraint B. Rogers
Summary: This study investigated the assembly of microbiome in C57BL/6 germ-free mice over 70 days, finding that multiple rounds of gavage led to increased taxonomic diversity, improved similarity to donor microbiota, and reduced within-group variance. However, gavaging with antibiotic-disrupted microbiota did not provide substantial benefits in terms of similarity to donor or variance reduction. The establishment of donor microbiota in recipient animals before experimentation is crucial and influenced by donor microbiota characteristics.
Article
Environmental Sciences
Madjid Djouina, Cecile Vignal, Alexandre Dehaut, Segolene Caboche, Nell Hirt, Christophe Waxin, Charlotte Himber, Delphine Beury, David Hot, Laurent Dubuquoy, David Launay, Guillaume Duflos, Mathilde Body-Malapel
Summary: The aim of this study was to investigate the effects of intestinal exposure to polyethylene (PE) microplastics on gut homeostasis. Mice were orally exposed to PE microbeads of two different sizes, and it was found that ingestion of PE microbeads led to intestinal disturbances and significant alterations of crucial intestinal markers. This highlights the need for further research on the health impact of microplastic exposure in humans.
ENVIRONMENTAL RESEARCH
(2022)
Review
Immunology
Olga Dremova, Maximilian Mimmler, Nadja Paeslack, My Phung Khuu, Zhenling Gao, Markus Bosmann, Lucien P. Garo, Nathalie Schoen, Alexa Mechler, Yunes Beneich, Vivian Rebling, Amrit Mann, Giulia Pontarollo, Klytaimnistra Kiouptsi, Christoph Reinhardt
Summary: In this article, the basic principles of germ-free isolator technology, various sterilization methods, and sterility testing methods for monitoring germ-free mouse colonies are reviewed. The effectiveness and limitations of these methods are discussed, along with the possible sources of isolator contamination and an overview of reported contaminants.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Food Science & Technology
Isabelle E. Logan, Natalia Shulzhenko, Thomas J. Sharpton, Gerd Bobe, Kitty Liu, Stephanie Nuss, Megan L. Jones, Cristobal L. Miranda, Stephany Vasquez-Perez, Jamie M. Pennington, Scott W. Leonard, Jaewoo Choi, Wenbin Wu, Manoj Gurung, Joyce P. Kim, Malcolm B. Lowry, Andrey Morgun, Claudia S. Maier, Jan F. Stevens, Adrian F. Gombart
Summary: Xanthohumol (XN) requires the intestinal microbiota to mediate its benefits in improving glucose and lipid metabolism, with changes in both microbial composition and functional capacity. The study found that XN supplementation decreased plasma insulin concentrations and improved insulin resistance in conventional mice, but not in germ-free mice. Furthermore, analysis indicated that XN supplementation altered microbial composition and led to the production of biologically active compounds by the intestinal microbiota.
MOLECULAR NUTRITION & FOOD RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Shengfeng Huang, Xin Tao, Shaochun Yuan, Yuhang Zhang, Peiyi Li, Helen A. Beilinson, Ya Zhang, Wenjuan Yu, Pierre Pontarotti, Hector Escriva, Yann Le Petillon, Xiaolong Liu, Shangwu Chen, David G. Schatz, Anlong Xu
Article
Immunology
Lisa K. Denzin, Aly A. Khan, Francesca Virdis, Jessica Wilks, Melissa Kane, Helen A. Beilinson, Stanislav Dikiy, Laure K. Case, Derry Roopenian, Michele Witkowski, Alexander V. Chervonsky, Tatyana V. Golovkina
Article
Cell Biology
Jeremie J. Gautier, Maria E. Lomakina, Lamia Bouslama-Oueghlani, Emmanuel Derivery, Helen Beilinson, Wolfgang Faigle, Damarys Loew, Daniel Louvard, Arnaud Echard, Antonina Y. Alexandrova, Buzz Baum, Alexis Gautreau
JOURNAL OF CELL SCIENCE
(2011)
Article
Virology
Jessica Wilks, Helen Beilinson, Betty Theriault, Alexander Chervonsky, Tatyana Golovkina
JOURNAL OF VIROLOGY
(2014)
Article
Multidisciplinary Sciences
Ryan M. Brecht, Catherine C. Liu, Helen A. Beilinson, Alexandra Khitun, Sarah A. Slavoff, David G. Schatz
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Virology
Emily Cullum, Stanislav Dikiy, Helen A. Beilmson, Melissa Kane, Alessandra Veinbachs, Vera M. Beilinson, Lisa K. Denzin, Alexander Chervonsky, Tatyana Golovkina
JOURNAL OF VIROLOGY
(2020)
Article
Immunology
Helen A. Beilinson, Rebecca A. Glynn, Anurupa Devi Yadavalli, Jianxiong Xiao, Elizabeth Corbett, Huseyin Saribasak, Rahul Arya, Charline Miot, Anamika Bhattacharyya, Jessica M. Jones, Jagan M. R. Pongubala, Craig H. Bassing, David G. Schatz
Summary: The RAG1 N-terminal region regulates V(D)J recombination and lymphocyte development through multiple pathways, including control of the balance between short- and long-range recombination, as shown in studies with different RAG1 mutant mice.
JOURNAL OF EXPERIMENTAL MEDICINE
(2021)
Article
Immunology
Chun-Chin Chen, Bo-Ruei Chen, Yinan Wang, Philip Curman, Helen A. Beilinson, Ryan M. Brecht, Catherine C. Liu, Ryan J. Farrell, Jaime de Juan-Sanz, Louis-Marie Charbonnier, Shingo Kajimura, Timothy A. Ryan, David G. Schatz, Talal A. Chatila, Jakob D. Wikstrom, Jessica K. Tyler, Barry P. Sleckman
Summary: SERCA proteins modulate intracellular Ca2+ levels to regulate RAG1 and RAG2 gene expression and V(D)J recombination, and defects in SERCA functions cause lymphopenia.
JOURNAL OF EXPERIMENTAL MEDICINE
(2021)
Article
Virology
Helen A. Beilinson, Amanda Sevilleja, Jessica Spring, Fernando Benavides, Vera Beilinson, Nickolas Neokosmidis, Tatyana Golovkina
Summary: Differential responses to viral infections are influenced by the genetic makeup of the host. Studies of resistance to retroviruses in human populations are complicated due to the inability to conduct proof-of-principle studies.
JOURNAL OF VIROLOGY
(2023)