A new approach to gene therapy usingSleeping Beautyto genetically modify clinical-grade T cells to target CD19

Toward eliminating HLA class I expression to generate universal cells from allogeneic donors

(2013) H. Torikai et al.   BLOOD

Sleeping Beauty System to Redirect T-cell Specificity for Human Applications

(2013) Sourindra N. Maiti et al.   JOURNAL OF IMMUNOTHERAPY

Bispecific T-cells Expressing Polyclonal Repertoire of Endogenous γδ T-cell Receptors and Introduced CD19-specific Chimeric Antigen Receptor

(2013) Drew C Deniger et al.   MOLECULAR THERAPY

Chimeric Antigen Receptor (CAR)-Specific Monoclonal Antibody to Detect CD19-Specific T Cells in Clinical Trials

(2013) Bipulendu Jena et al.   PLoS One

Manufacture of Clinical-Grade CD19-Specific T Cells Stably Expressing Chimeric Antigen Receptor Using Sleeping Beauty System and Artificial Antigen Presenting Cells

(2013) Harjeet Singh et al.   PLoS One

Evaluating risks of insertional mutagenesis by DNA transposons in gene therapy

(2013) Perry B. Hackett et al.   Translational Research

NaïVe Cd19-Specific T Cells Exhibit Superior Proliferation and Potential for Adoptive Immunotherapy

(2012) H. Singh et al.   BIOLOGY OF BLOOD AND MARROW TRANSPLANTATION

A foundation for universal T-cell based immunotherapy: T cells engineered to express a CD19-specific chimeric-antigen-receptor and eliminate expression of endogenous TCR

(2012) H. Torikai et al.   BLOOD

A New Approach to Simultaneously Quantify Both TCR  - and  -Chain Diversity after Adoptive Immunotherapy

(2012) M. Zhang et al.   CLINICAL CANCER RESEARCH

Efficacy and Safety of Sleeping Beauty Transposon-Mediated Gene Transfer in Preclinical Animal Studies

(2012) Perry B. Hackett et al.   CURRENT GENE THERAPY

Imaging of genetically engineered T cells by PET using gold nanoparticles complexed to Copper-64

(2012) Parijat Bhatnagar et al.   Integrative Biology

Hyperactive self-inactivating piggyBac for transposase-enhanced pronuclear microinjection transgenesis

(2012) J. Marh et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Reprogramming CD19-Specific T Cells with IL-21 Signaling Can Improve Adoptive Immunotherapy of B-Lineage Malignancies

(2011) H. Singh et al.   CANCER RESEARCH

The hyperactive Sleeping Beauty transposase SB100X improves the genetic modification of T cells to express a chimeric antigen receptor

(2011) Z Jin et al.   GENE THERAPY

The Sleeping Beauty transposon system: a non-viral vector for gene therapy

(2011) E. L. Aronovich et al.   HUMAN MOLECULAR GENETICS

A high-throughput single-cell analysis of human CD8+ T cell functions reveals discordance for cytokine secretion and cytolysis

(2011) Navin Varadarajan et al.   JOURNAL OF CLINICAL INVESTIGATION

PiggyBac-mediated Cancer Immunotherapy Using EBV-specific Cytotoxic T-cells Expressing HER2-specific Chimeric Antigen Receptor

(2011) Yozo Nakazawa et al.   MOLECULAR THERAPY

Chimeric Antigen Receptor–Modified T Cells in Chronic Lymphoid Leukemia

(2011) David L. Porter et al.   NEW ENGLAND JOURNAL OF MEDICINE

T Cells with Chimeric Antigen Receptors Have Potent Antitumor Effects and Can Establish Memory in Patients with Advanced Leukemia

(2011) M. Kalos et al.   Science Translational Medicine

Translating Sleeping Beauty transposition into cellular therapies: Victories and challenges

(2010) Zsuzsanna Izsvák et al.   BIOESSAYS

Adoptive Immunotherapy After Umbilical Cord Blood (UCB) Transplantation: Manufacturing And Analysis Of CD19-Specific UCB-Derived T-cells From Scant Numbers Of UCB Mononuclear Cells

(2010) S.S. Kelly et al.   BIOLOGY OF BLOOD AND MARROW TRANSPLANTATION

Combining CD19 Redirection and Alloanergization to Generate Tumor-Specific Human T Cells for Allogeneic Cell Therapy of B-Cell Malignancies

(2010) J. K. Davies et al.   CANCER RESEARCH

K562/GM-CSF Immunotherapy Reduces Tumor Burden in Chronic Myeloid Leukemia Patients with Residual Disease on Imatinib Mesylate

(2010) B. D. Smith et al.   CLINICAL CANCER RESEARCH

A Transposon and Transposase System for Human Application

(2010) Perry B Hackett et al.   MOLECULAR THERAPY

Emerging potential of transposons for gene therapy and generation of induced pluripotent stem cells

(2009) T. VandenDriessche et al.   BLOOD

Immunotherapy of Metastatic Melanoma Using Genetically Engineered GD2-Specific T cells

(2009) E. Yvon et al.   CLINICAL CANCER RESEARCH

The promise and potential pitfalls of chimeric antigen receptors

(2009) Michel Sadelain et al.   CURRENT OPINION IN IMMUNOLOGY

Efficient nonviral Sleeping Beauty transposon-based TCR gene transfer to peripheral blood lymphocytes confers antigen-specific antitumor reactivity

(2009) P D Peng et al.   GENE THERAPY

piggyBac Transposon/Transposase System to Generate CD19-Specific T Cells for the Treatment of B-Lineage Malignancies

(2009) Pallavi V. Raja Manuri et al.   HUMAN GENE THERAPY

A Herceptin-Based Chimeric Antigen Receptor with Modified Signaling Domains Leads to Enhanced Survival of Transduced T Lymphocytes and Antitumor Activity

(2009) Y. Zhao et al.   JOURNAL OF IMMUNOLOGY

Molecular evolution of a novel hyperactive Sleeping Beauty transposase enables robust stable gene transfer in vertebrates

(2009) Lajos Mátés et al.   NATURE GENETICS

Adoptively transferred effector cells derived from naive rather than central memory CD8+ T cells mediate superior antitumor immunity

(2009) C. S. Hinrichs et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Redirecting Specificity of T-Cell Populations For CD19 Using the Sleeping Beauty System

(2008) H. Singh et al.   CANCER RESEARCH

PET imaging of T cells derived from umbilical cord blood

(2008) H Singh et al.   LEUKEMIA

Characterization of intracellular HLA-DR, DM and DO profile in K562 and HL-60 leukemic cells

(2008) Lina Papadimitriou et al.   MOLECULAR IMMUNOLOGY

Transcriptional Activities of the Sleeping Beauty Transposon and Shielding Its Genetic Cargo With Insulators

(2007) Oliver Walisko et al.   MOLECULAR THERAPY