Review
Chemistry, Multidisciplinary
Mahmoud A. Alshahat, Mohamed A. Elgenedy, Ahmed A. Aboushady, Mark T. S. Williams
Summary: This paper reviews the use of Tumour Treating Field (TTF) treatment for cancer and compares different voltage pulse generators in terms of treatment efficacy and efficient use of electrical power.
APPLIED SCIENCES-BASEL
(2023)
Article
Biochemistry & Molecular Biology
Romanos Poulkouras, Gerwin Dijk, Marie Lefevre, Martin Baca, David Moreau, Rodney P. O'Connor
Summary: A study found that coating metal electrodes with an organic polymer PEDOT:PSS can prevent intracellular ROS generation and reduce cell electroporation and intracellular calcium response caused by PEF application. These coated electrodes can be used for PEF application in ROS-sensitive tissues and differentiate the effects of PEFs on cells from the metabolic impact of electrolytic events.
BIOELECTROCHEMISTRY
(2022)
Article
Food Science & Technology
Se-Ho Jeong, Young-Min Jung, Siyeon Kim, Jong-Hun Kim, Hyunho Yeo, Dong-Un Lee
Summary: This study investigated the effects of pulse electric field (PEF) treatment on the tenderization of beef semitendinosus muscle. An adjustable PEF chamber was designed to directly contact the beef sample without water as the transmitting medium. PEF treatment induced structural changes in beef muscle, and the degree of changes depended on the electric field strength. At 2.0 kV/cm, cutting force, hardness, and chewiness significantly decreased, and troponin-T was extensively degraded. Under a certain intensity (1.0 kV/cm), PEF treatment retained the fresh quality factors such as color and lipid oxidation.
Article
Chemistry, Multidisciplinary
Dana Middendorf, Ute Bindrich, Claudia Siemer, Stefan Toepfl, Volker Heinz
Summary: The study demonstrates the potential of using pulsed electrical fields (PEF) to modify the structure of casein micelles, allowing for the incorporation of lipophilic bioactive substances. The research shows that PEF can disintegrate casein micelles and facilitate the integration of significant amounts of beta-carotene. Additionally, a combination of PEF and thermal treatment can be used to restructure the micelles and efficiently trap beta-carotene, achieving an encapsulation efficiency of 78%.
APPLIED SCIENCES-BASEL
(2021)
Article
Polymer Science
Ahmed Taha, Federico Casanova, Martynas Talaikis, Voitech Stankevic, Nerija Zurauskiene, Povilas Simonis, Vidas Pakstas, Marijus Jurkunas, Mohamed A. E. Gomaa, Arunas Stirke
Summary: Pulsed electric field (PEF) as a green processing technology can change the structure and physicochemical properties of casein micelles (CSMs), enhancing their techno-functional properties. The effects of different electric field strengths on CSMs were investigated, showing that particle size, surface hydrophobicity, and protein solubility were influenced by PEF treatment. Moreover, PEF treatment affected the fluorescence spectrum and secondary structure of CSMs, as well as disulfide linkages. Overall, PEF technology has the potential to improve CSMs' properties and contribute to sustainable development goals.
Article
Food Science & Technology
Tong-Ge Bai, Liang Zhang, Jian-Ya Qian, Wei Jiang, Mangang Wu, Sheng-Qi Rao, Qian Li, Chen Zhang, Chunsen Wu
Summary: PEF pretreatment has significant impacts on the properties of rice, including increased starch hydrolysis degree and hardness, decreased integrity of cell structure, and altered chemical composition of cooked rice.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Engineering, Biomedical
S. Kara, C. P. Uzunoglu, E. T. Isci, F. Atalar, M. Ugur
Summary: Electromagnetic field (EMF) applications have long been used in medical therapy for various nerve diseases and tissue regeneration. In this study, an amplifier circuit was designed to generate proposed pulsed electromagnetic fields (PEMF) for controlled and directional growth of Dorsal Root Ganglia (DRG) nerve cells. The use of a novel asymmetrical waveform (sawtooth) along with triangular and square waveforms was employed to effectively elongate nerve cells. The effectiveness of the proposed system was verified through investigation of morphological nerve cell elongation results and PEMF results.
BIOMEDICAL SIGNAL PROCESSING AND CONTROL
(2023)
Article
Chemistry, Multidisciplinary
Justina Jurgeleviciute, Nedas Bickovas, Andrius Sakalauskas, Vitalij Novickij, Vytautas Smirnovas, Egle Lastauskiene
Summary: Prions are misfolded proteins that can cause brain diseases, and applying a pulsed electric field can disintegrate their aggregates; Yeasts are ideal models for studying prions and it is found that prions can significantly increase cell survivability.
APPLIED SCIENCES-BASEL
(2021)
Article
Engineering, Chemical
Barbara Surano, Graciela Leiva, Guillermo Marshall, Felipe Maglietti, Carolina Schebor
Summary: By utilizing PEF technology, natural pigments and bioactive compounds can be effectively extracted from prickly pear fruit, resulting in increased juice yield and nutritional properties, suitable for industrial applications.
JOURNAL OF FOOD ENGINEERING
(2022)
Article
Food Science & Technology
Si-Yeon Kim, Byung-Min Lee, Seok-Young Hong, Hyun-Ho Yeo, Se-Ho Jeong, Dong-Un Lee
Summary: This study investigated the effects of pulsed electric field (PEF) treatment on the drying and rehydration kinetics, microstructure, and carotenoid content of carrot. The results showed that PEF treatment reduced drying time, improved rehydration ability, and preserved carotenoid content.
Article
Environmental Sciences
Fan Wang, Liang Li, Xuejie Li, Xiaomin Hu, Bo Zhang
Summary: The purpose of this study was to explore the stimulation mechanism of low pulsed electric field (PEF) strength treatment to promote the growth metabolism of aerobic denitrifying bacteria Pseudomonas putida W207-14. The results showed that PEF treatment can shorten the growth time, increase the metabolic rate, and promote nutrient transport and electron transfer, thus promoting the growth metabolism of the bacteria.
ENVIRONMENTAL TECHNOLOGY
(2023)
Article
Chemistry, Analytical
Isaac Aaron Rodriguez Osuna, Pablo Cobelli, Nahuel Olaiz
Summary: This study focuses on the local variations of the electric field and current density around bubbles formed by water electrolysis using PEF technology, and how these variations affect the electroporation protocol. It is found that the edge effect around the bubbles can significantly increase the electric field and current density, leading to unwanted limitations on PEF treatment.
Article
Oncology
Seung Jeong, Song Hee Kim, Hongbae Kim, Jeon Min Kang, Yubeen Park, Dong-Sung Won, Ji Won Kim, Dae Sung Ryu, Chu Hui Zeng, Jong Hoon Chung, Bumjin Lim, Jung-Hoon Park
Summary: HF-PEF is a safe and effective method for inducing ablation and tissue regeneration in prostate cancer.
Article
Cardiac & Cardiovascular Systems
Atul E. Verma, David V. Haines, Lucas Boersma, Nitesh Sood, Andrea E. Natale, Francis Marchlinski, Hugh Calkins, Prashanthan L. Sanders, Douglas Packer, Karl-Heinz Kuck, Gerhard Hindricks, Birce Onal, Jeffrey Cerkvenik, Hiroshi B. Tada, David DeLurgio, PULSED AF Investigators
Summary: Pulsed field ablation is an effective and safe method for treating patients with atrial fibrillation. It has comparable effectiveness to traditional catheter ablation but with a lower rate of complications.
Article
Food Science & Technology
Artur Wiktor, Ales Landfeld, Aleksandra Matys, Pavla Novotna, Magdalena Dadan, Eliska Kovarikova, Malgorzata Nowacka, Martin Mulenko, Dorota Witrowa-Rajchert, Jan Strohalm, Milan Houska
Summary: This study aimed to compare the physicochemical properties of 'Golden Delicious' apples treated with high-pressure processing, ultrasound, or pulsed electric field. The results showed that different pretreatment methods can shape the properties of the apples, with pulsed electric field being the most effective in improving water uptake during rehydration and high-pressure processing being the most effective in reducing hygroscopic properties. Overall, pretreated apples had lower total phenolic content compared to the reference sample, with sonicated samples showing the smallest decrease.
Article
Biochemistry & Molecular Biology
Damaris Krust, Christian Gusbeth, Alexander S. K. Mueller, Daniel Scherer, Georg Mueller, Wolfgang Frey, Peter Nick
Summary: Research has shown that PEF treatment induces cell death in Chlorella vulgaris and releases a cell-death inducing factor. Subsequent incubation after PEF treatment allows for the extraction of this inducing factor from treated microalgae, serving as an effective strategy for extracting soluble proteins and lipids.
BIOELECTROCHEMISTRY
(2022)
Article
Agricultural Engineering
Ronald Halim, Ioannis Papachristou, George Q. Chen, Huining Deng, Wolfgang Frey, Clemens Posten, Aude Silve
Summary: Novel cell-disruption combinations were used to study the mass transfer of lipids and proteins in Nannochloropsis slurries. The transfer of neutral lipids depended on the disintegration of cell walls, while the transfer of proteins was influenced by both cell-wall disintegration and protein linkage severance. Combinations that primarily involved physical disruption released soluble proteins, while combinations that hydrolysed chemical linkages in addition to physical disruption released both soluble and structural proteins.
BIORESOURCE TECHNOLOGY
(2022)
Article
Plant Sciences
Leonie Hacker, Niklas Capdeville, Laura Feller, Janina Enderle-Kukla, Annika Dorn, Holger Puchta
Summary: WSS1A is crucial for repair of DNA-protein crosslinks in plants; Loss of WSS1A leads to reduced rDNA repeats and chromosomal fragmentation; WSS1A is involved in suppressing DSB formation or in DSB repair itself, especially in the absence of other factors from the RTR complex.
Article
Biochemistry & Molecular Biology
Leonie Hacker, Annika Dorn, Janina Enderle, Holger Puchta
Summary: The research reveals that Arabidopsis thaliana has two main repair pathways for TOP2cc, mediated by TDP2 and WSS1A, while MUS81 plays a major role in global DPC repair. Additionally, TDP1 and TDP2 function in TOP1cc repair, preventing erroneous pathways.
Review
Biochemical Research Methods
Niklas Capdeville, Patrick Schindele, Holger Puchta
Summary: Since their first adaptation for plant genome editing, CRISPR-Cas system nucleases and tools have revolutionized the field, enabling precise induction of predefined modifications and the generation of more efficient base editors. Prime editors have also been optimized for plant applications, allowing accurate substitutions, insertions, and deletions. Recent progress in chromosome restructuring allows not only genetic linkages and breakage, but also promoter swapping.
CURRENT OPINION IN BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
I Papachristou, S. Zhang, O. Gorte, K. Ochsenreither, R. Wustner, N. Nazarova, W. Frey, A. Silve
Summary: This study evaluated the efficiency of direct transesterification after pulsed electric field (PEF) treatment for two different cultivation modes of freshly harvested Auxenochlorella protothecoides. The results showed that PEF treatment significantly improved the recovery of fatty acid methyl esters (FAMEs) for autotrophic microalgae, but not for mixotrophic microalgae. Further investigation is needed to understand the reason behind this different behavior.
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Patrick Schindele, Laura Merker, Tom Schreiber, Anja Prange, Alain Tissier, Holger Puchta
PLANT BIOTECHNOLOGY JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Eva Dvorak Tomastikova, Klara Prochazkova, Fen Yang, Jitka Jemelkova, Andreas Finke, Annika Dorn, Mahmoud Said, Holger Puchta, Ales Pecinka
Summary: DNA-protein cross-links (DPCs) are highly toxic DNA lesions that block DNA replication and transcription. Arabidopsis has three pathways to repair DPCs: endonucleolytic cleavage, proteolytic degradation, and cleavage of phosphodiester bonds. The SMC5/6 complex is identified as an important factor in plant DPC repair through a genetic screen.
Article
Biotechnology & Applied Microbiology
Ralf Straessner, Marcell Nikolausz, Aude Silve, Natalja Nazarova, Ruediger Wuestner, Ioannis Papachristou, Sahar Akaberi, Klaus Leber, Georg Mueller, Wolfgang Frey
Summary: This study proposes a cascade processing method for utilizing lipid-rich microalgae Auxenochlorella protothecoides. The method includes pre-concentration of biomass, pretreatment using pulsed electric fields (PEF), separation of aqueous fraction for recovery of valuable components, and lipid extraction from the remaining wet pellet. The remaining biomass is then subjected to anaerobic digestion (AD) for biogas production. The study demonstrates the feasibility and potential of this approach for industrial implementation.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2023)
Article
Plant Sciences
Fabienne Gehrke, Paola Ruiz-Duarte, Angelina Schindele, Sebastian Wolf, Holger Puchta
Summary: The application of the CRISPR/Cas system as a biotechnological tool for genome editing has revolutionized plant biology. Recently, the repertoire was expanded by CRISPR-Kill, enabling CRISPR/Cas-mediated tissue engineering through genome elimination by tissue-specific expression. This new system not only provides spatial control by tissue-specific expression but also allows temporal control of CRISPR-mediated cell death in Arabidopsis thaliana.
Article
Biotechnology & Applied Microbiology
Janine Pietralla, Niklas Capdeville, Patrick Schindele, Holger Puchta
Summary: ErCas12a is a nuclease derived from Eubacterium rectale with potential applications in plant genome engineering. Optimized through protein engineering, an improved variant named imErCas12a showed significantly enhanced gene editing efficiency in comparison to the wild-type enzyme. This economically attractive alternative demonstrated strong editing efficiencies at lower temperatures and enabled editing of previously inaccessible targets without off-site activity.
PLANT BIOTECHNOLOGY JOURNAL
(2023)
Review
Plant Sciences
Holger Puchta, Andreas Houben
Summary: Spontaneous chromosomal rearrangements (CRs) play a vital role in speciation, genome evolution and crop domestication. Chromosome engineering, through methods such as chromosomal translocations, inversion reversions, and construction of minichromosomes, has the potential to modify genetic linkage groups, change the number of chromosomes, and establish genetic isolation, thus aiding breeding and plant biotechnology.
Article
Plant Sciences
Michelle Roenspies, Carla Schmidt, Patrick Schindele, Michal Lieberman-Lazarovich, Andreas Houben, Holger Puchta
Summary: This study demonstrates the suppression of meiotic recombination through chromosome restructuring, inducing a heritable inversion and discovering that genetic exchange mainly occurs at telomeric ends.
Article
Plant Sciences
Michelle Roenspies, Holger Puchta
Summary: A major challenge in plant breeding is the establishment or breakage of genetic linkages through random meiotic recombination. This problem can be addressed by utilizing CRISPR-Cas-mediated chromosome engineering. Researchers found that by inverting a segment of chromosome 2 in Arabidopsis thaliana, they were able to effectively suppress genetic crossovers in that region.
Article
Plant Sciences
Fabienne Gehrke, Angelina Schindele, Holger Puchta
Summary: Heritable plant chromosome engineering can be achieved in somatic cells using CRISPR/Cas to induce nonhomologous double-strand break repair pathways. This technology allows for large-scale restructuring of plant chromosomes, including duplications, inversions, and translocations. The use of nonhomologous end joining pathways in somatic cells facilitates efficient chromosomal rearrangements. This breakthrough has the potential to revolutionize plant breeding.