Article
Medicine, Research & Experimental
Keisuke Nakagawa, Mayuko Itoya, Nao Takemoto, Yuika Matsuura, Masashi Tawa, Yasuo Matsumura, Mamoru Ohkita
Summary: This study showed that IS increases O-2(-) production in vascular tissues, attenuating ACh- and SNP-induced vasorelaxation via potential NO inactivation. Additionally, IS-promoted O-2(-) production in the presence of vascular endothelium is likely through binding to AhR and activation of NADPH oxidase.
Article
Biochemistry & Molecular Biology
Volodymyr Lushchak, Oleh Lushchak
Summary: In living organisms, most oxygen is reduced to water through four-electron reduction, but a small percentage is reduced through one-electron mechanisms to produce reactive oxygen and nitrogen species. These species interact with various components of living organisms, leading to diverse effects on biological systems.
CHEMICO-BIOLOGICAL INTERACTIONS
(2021)
Article
Agriculture, Dairy & Animal Science
Giuseppina Basini, Simona Bussolati, Simone Bertini, Fausto Quintavalla, Francesca Grasselli
Summary: The study identified that triclosan disrupts cellular function, particularly interfering with hormone production and cell proliferation, suggesting a critical evaluation of its effects.
Article
Plant Sciences
Agnieszka Wal, Pawel Staszek, Barbara Pakula, Magdalena Paradowska, Urszula Krasuska
Summary: This study investigated the levels of superoxide anion, nitric oxide, and peroxynitrite in the digestive fluid of the carnivorous pitcher plant. The presence of reactive oxygen species and reactive nitrogen species was found, and their occurrence was related to the scavenging capacity and phenolics content. Nitric oxide emission was higher in the fed traps compared to the unfed ones, and the concentration of nitrite decreased in the fed traps, indicating it as the main source of nitric oxide. The presence of ROS and RNS was observed even in closed traps, suggesting their involvement in both digestion and signaling during trap development.
Review
Developmental Biology
Cristian O'Flaherty, Eleonora Scarlata
Summary: This review focuses on the enzymatic antioxidant mechanisms of spermatozoa to combat oxidative stress, highlighting the differences among mammalian species. The balance between reactive oxygen species (ROS) production and antioxidant enzymes is crucial for sperm capacitation and quality. Understanding the molecular mechanisms affected by oxidative stress and developing personalized antioxidant therapy are of paramount importance for diagnosing and treating male infertility associated with oxidative damage.
Article
Biochemistry & Molecular Biology
G. Massimo, R. S. Khambata, T. Chapman, K. Birchall, C. Raimondi, A. Shabbir, Nicki Dyson, K. S. Rathod, C. Borghi, A. Ahluwalia
Summary: XDH mutations have varying impacts on the biochemical activity of the enzyme, leading to potential harmful or protective influences on health. Targeting enzyme activity to enhance the nitrite-reductase profile may provide novel therapeutic options, especially in cardiovascular disease.
Article
Cell Biology
Maria Jose Gomez-Torres, Natalia Huerta-Retamal, Laura Robles-Gomez, Paula Saez-Espinosa, Jon Aizpurua, Manuel Aviles, Alejandro Romero
Summary: Capacitation induces changes in sperm surface ARSA density and redistribution, with a progressive clustering in the peri-acrosomal region over time, indicating a time-dependent capacitation-mediated translocation of ARSA residues on sperm surfaces. These findings offer new insights into the molecular events preceding sperm-oocyte interactions.
Article
Biochemistry & Molecular Biology
Marie Bisconti, Philippe Grosjean, Vanessa Arcolia, Jean-Francois Simon, Elise Hennebert
Summary: In order to study fertility mechanisms, experiments are conducted using solvents such as ethanol (EtOH) or dimethyl sulfoxide (DMSO) to incubate spermatozoa in the presence of dissolved molecules. However, the effect of the solvents on sperm parameters and the outcome of the experiments is often overlooked. This study investigated the impact of EtOH and DMSO on sperm motility, vitality, capacitation, and acrosome integrity, and found significant relationships between increasing solvent concentrations and these parameters.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Joao C. Ribeiro, Raquel L. L. Bernardino, Ana Goncalves, Alberto Barros, Giuseppe Calamita, Marco G. G. Alves, Pedro F. F. Oliveira
Summary: Osmoregulation is essential for sperm function, and aquaglyceroporins play a crucial role in glycerol permeability, which is important for sperm motility. AQP7 is especially significant in glycerol transport, and its impairment may lead to reduced sperm motility in asthenozoospermic individuals.
Article
Pharmacology & Pharmacy
Keisuke Nakagawa, Fumika Kobayashi, Yoshiki Kamei, Masashi Tawa, Mamoru Ohkita
Summary: The accumulation of uremic toxin kynurenine impairs vascular endothelial function and increases O-2(center dot-) production, leading to reduced vasorelaxation ability.
BIOLOGICAL & PHARMACEUTICAL BULLETIN
(2022)
Article
Biochemistry & Molecular Biology
Maria Jose Gomez-Torres, Paula Saez-Espinosa, Paula Manzano-Santiago, Laura Robles-Gomez, Natalia Huerta-Retamal, Jon Aizpurua
Summary: This study characterized the distribution of SPAM1 in different physiological conditions of human sperm and found that the presence of SPAM1 throughout the sperm head is necessary for proper contact with the cumulus-oocyte matrix.
Article
Reproductive Biology
Yael Azoulay, Zvi Malik, Haim Breitbart
Summary: Bacterial contamination in semen can deteriorate sperm function, potentially by inducing a premature form of the acrosome reaction. To understand the mechanism, the effects of bacteria on sperm acrosome reaction and other parameters involved in sperm capacitation were studied.
Review
Cell Biology
Bhupesh Singla, Ravi Varma Aithabathula, Sonia Kiran, Shweta Kapil, Santosh Kumar, Udai P. Singh
Summary: The lymphatic system plays a crucial role in immune surveillance and tissue homeostasis, and lymphangiogenesis, the formation of new lymphatic vessels, is involved in various physiological and pathological processes. Recent research progress in the molecular mechanisms of lymphangiogenesis has opened up new possibilities for therapeutic approaches in diseases such as neurological disorders, cardiac repair, autoimmune disease, obesity, and atherosclerosis.
Article
Biochemistry & Molecular Biology
Sarah Grassi, Marie Bisconti, Baptiste Martinet, Vanessa Arcolia, Jean-Francois Simon, Ruddy Wattiez, Baptiste Leroy, Elise Hennebert
Summary: This study found that the relative abundance of all detected isoforms of HSP70 remains stable during sperm capacitation and is not correlated with sperm motility.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Physiology
Lucia Piacenza, Ari Zeida, Madia Trujillo, Rafael Radi
Summary: The free radical nitric oxide (NO) plays a key role in physiological processes, but its bioavailability is compromised in certain disease conditions due to increased production of superoxide radical. This leads to the inactivation of nitric oxide and the formation of peroxynitrite, which is implicated in various human diseases and the aging process. Understanding the complex biochemistry of peroxynitrite is crucial for developing therapeutic interventions.
PHYSIOLOGICAL REVIEWS
(2022)