Article
Cell Biology
Sheng An, Yi Yao, Hongbin Hu, Junjie Wu, Jiaxin Li, Lulan Li, Jie Wu, Maomao Sun, Zhiya Deng, Yaoyuan Zhang, Shenhai Gong, Qiaobing Huang, Zhongqing Chen, Zhenhua Zeng
Summary: The increase of lactate is an independent risk factor for sepsis-induced acute kidney injury (SAKI). The downregulation of deacetylase Sirtuin 3 (SIRT3) leads to excessive lactate production and exacerbates renal injury. Through the regulation of lactate levels and mitochondrial protein Fis1 lactylation, the severity of SAKI can be alleviated.
CELL DEATH & DISEASE
(2023)
Article
Genetics & Heredity
YanYan Ma, YaoGang Zhang, Tao Zhang, Zhu Man, XiaoMing Su, ShuJing Hao, TianZe Wang
Summary: This study identified a practical enzymatic diagnostic method for PDHC-related mitochondrial diseases through peripheral blood leukocyte PDHC activity assay and gene mutation analysis. The results showed a dramatically decreased PDHC activity and the identification of a missense mutation in the PDHC gene, providing insights for clinical diagnosis of PDHC deficiency.
MOLECULAR GENETICS & GENOMIC MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Franziska Alfen, Elena Putscher, Michael Hecker, Uwe Klaus Zettl, Andreas Hermann, Jan Lukas
Summary: Fabry disease is a rare genetic disorder caused by mutations in the GLA gene. This study investigated the impact of these mutations on splicing behavior and found that abnormal splicing can lead to reduced enzyme activity and affect treatment options. Experimental analysis confirmed alternative splicing events for specific mutations, highlighting the importance of splicing phenotype analysis in studying exonic GLA gene mutations.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Genetics & Heredity
Qing Xin, Qihua Liu, Zhiying Liu, Xiaomeng Shi, Xuyan Liu, Ruixiao Zhang, Yefeng Hong, Xiangzhong Zhao, Leping Shao
Summary: This study aims to investigate the consequences of exonic mutations associated with Bartter syndrome (BS) on pre-mRNA splicing. The results showed that 12 out of 14 candidate variants were identified to induce splicing alterations. These findings provide important insights for assessing the consequences of exonic variants.
FRONTIERS IN GENETICS
(2022)
Article
Biochemistry & Molecular Biology
Kangli Peng, Shirong Wang, Ruochuan Liu, Li Zhou, Geon H. Jeong, In Ho Jeong, Xianpeng Liu, Hiroaki Kiyokawa, Bingzhong Xue, Bo Zhao, Hang Shi, Jun Yin
Summary: This study identified a novel mechanism for regulating glycolysis and lipid metabolism through UBE3A-mediated ubiquitination of PDHA1 and ACAT1. A high-fat diet suppressed UBE3A expression in the liver, leading to increased ACAT1 protein levels and promoting the development of NAFLD.
Article
Biochemistry & Molecular Biology
Huan Chen, Kunming Liang, Cong Hou, Hai-long Piao
Summary: Mammalian target of rapamycin (mTOR) controls cellular anabolism and its hyperactivation in cancer cells. Rapamycin, an FDA-approved drug, inhibits mTOR signaling and has been used for cancer treatment. However, it inhibits cancer growth rather than inducing apoptosis.
JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B
(2023)
Article
Biochemistry & Molecular Biology
Kazuhiro Maeta, Manal Farea, Hisahide Nishio, Masafumi Matsuo
Summary: This study identified a novel antisense oligonucleotide called KMM001 that inhibits the maturation of myostatin mRNA, resulting in decreased myostatin protein levels. KMM001 also inhibits SMAD-mediated myostatin signaling and promotes human myoblast proliferation. These findings suggest that KMM001 has potential therapeutic applications for muscle-wasting conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
W. Chris Moxley, Mark A. Eiteman
Summary: Sucrose, an abundant and renewable carbohydrate, is a promising feedstock for biotechnological production of chemicals. Escherichia coli W strain, with the deletion of the csc regulon repressor and expression of a variant E1 component of the pyruvate dehydrogenase complex, exhibited efficient pyruvate production from sucrose. The engineered strain, AceE[H106M;E401A], achieved a high pyruvate yield of 0.68 g/g sucrose with a volumetric productivity of 1.6 g/L.h, demonstrating the feasibility of sucrose-based pyruvate production with comparable performance to glucose-based processes.
FERMENTATION-BASEL
(2023)
Article
Nutrition & Dietetics
Song Hee Lee, Bo Young Choi, A. Ra Kho, Dae Ki Hong, Beom Seok Kang, Min Kyu Park, Si Hyun Lee, Hui Chul Choi, Hong Ki Song, Sang Won Suh
Summary: The combined treatment of pyruvate and DCA shows protective effects against seizure-induced hippocampal neuronal cell death by increasing the proportion of pyruvate converted to ATP.
Article
Biotechnology & Applied Microbiology
Cheng-Kai Shiau, Jia-Hsin Huang, Yu-Ting Liu, Huai-Kuang Tsai
Summary: This study provides the first genome-wide identification of enhancer-AS associations in human and mouse, showing that a significant portion of enhancers play roles in AS regulations. The results and the proposed platform VEnAS offer a deeper understanding of enhancers in regulating alternative splicing.
Article
Biochemistry & Molecular Biology
Nives Skorja Milic, Klemen Dolinar, Katarina Mis, Urska Matkovic, Marusa Bizjak, Mojca Pavlin, Matej Podbregar, Sergej Pirkmajer
Summary: Inhibition of pyruvate dehydrogenase kinase (PDK) by Dichloroacetate (DCA) can reduce the abundance of PDK isoenzymes in a cell-type-specific manner via both transcriptional and post-transcriptional mechanisms. The differential response of PDK isoenzymes to DCA in cancer cells and muscle cells highlights the importance of understanding the pharmacological effects of DCA in different cell types.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Pharmacology & Pharmacy
Langmei Deng, Anqi Jiang, Hanqing Zeng, Xiaoji Peng, Liying Song
Summary: Recent studies have found that the abnormal expression of PDHA1 is associated with the prognosis of various cancers. High expression of PDHA1 is correlated with poor prognosis in lung adenocarcinoma, while low expression of PDHA1 is associated with poor prognosis in kidney renal clear cell carcinoma. However, in stomach adenocarcinoma, downregulation of PDHA1 predicts a good prognosis. Additionally, the expression of PDHA1 is negatively correlated with tumor-infiltrating immune cells.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Cardiac & Cardiovascular Systems
Qinfeng Li, Chao Li, Abdallah Elnwasany, Gaurav Sharma, Yu A. An, Guangyu Zhang, Waleed M. Elhelaly, Jun Lin, Yingchao Gong, Guihao Chen, Meihui Wang, Shangang Zhao, Chongshan Dai, Charles D. Smart, Juan Liu, Xiang Luo, Yingfeng Deng, Lin Tan, Shuang-Jie Lv, Shawn M. Davidson, Jason W. Locasale, Philip L. Lorenzi, Craig R. Malloy, Thomas G. Gillette, Matthew G. Vander Heiden, Philipp E. Scherer, Luke I. Szweda, Guosheng Fu, Zhao V. Wang
Summary: PKM1 plays a crucial role in maintaining a homeostatic response in the heart under hemodynamic stress, protecting against cardiac dysfunction and fibrosis caused by pressure overload. Furthermore, PKM1 is essential for enhancing glycolytic flux, mitochondrial respiration, and ATP production under pressure overload at a mechanistic level.
Review
Medicine, Research & Experimental
Russel J. Reiter, Ramaswamy Sharma, Carmen Rodriguez, Vanesa Martin, Sergio Rosales-Corral, Debora Aparecida Pires de Campos Zuccari, Luiz Gustavo de Almeida Chuffa
Summary: This paper explores the relationship between endogenous melatonin and solid tumors, highlighting the potential of using melatonin to influence cancer cell metabolism. It suggests that solid tumors exhibit a healthier metabolic state at night, potentially reducing proliferation and invasiveness. The paper also proposes using melatonin in a time-sensitive treatment paradigm and combining it with traditional chemotherapy for improved outcomes in cancer treatment.
Article
Biotechnology & Applied Microbiology
Tao Si, Xuejian Ning, Hongwei Zhao, Mingmin Zhang, Ping Huang, Zhengguo Hu, Liu Yang, Lizhu Lin
Summary: The study found that miR-9-5p promotes proliferation, invasion, and migration of HCC cells by regulating the expression of PDK4, enhancing mitochondrial activity and energy metabolism, while suppressing apoptosis, ultimately increasing tumorigenicity in mouse xenograft models. This suggests that miR-9-5p and PDK4 could be potential therapeutic targets for preventing recurrence and metastasis of HCC.
CANCER GENE THERAPY
(2021)