Review
Biochemistry & Molecular Biology
Nora Tula Detering, Tobias Schuening, Niko Hensel, Peter Claus
Summary: Spinal muscular atrophy (SMA) is a disease caused by low levels of survival of motoneuron (SMN) protein. Phosphorylation of SMN is considered a key factor affecting SMN function in SMA. Phosphorylation can influence the localization, stability, and functions of SMN, making it a potential important target in SMA treatment strategies.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Neurosciences
Takae Ohno, Satoshi Fukuda, Naoyuki Murabe, Mizuho Niido, Masaki Sakurai
Summary: This study used viral tracing and slice recording techniques to investigate the development and elimination of early corticomotoneuronal connections. The findings revealed that these connections increased gradually from the second week postnatal to the twentieth week before rapidly disappearing, with the timing of elimination matching previous research results.
Article
Oncology
Rohan Wishard, Mohan Jayaram, Saraf R. Ramesh, Upendra Nongthomba
Summary: In this study, two isoforms of the myofibrillar protein Mlp60A were identified in Drosophila, with the short isoform being expressed during development and the long isoform specific to adults. Knockdown of both isoforms resulted in developmental defects and flight impairment in flies. Complete loss of both isoforms led to abnormal development and muscle defects. Overexpression of the short isoform partially rescued the developmental lethality.
EXPERIMENTAL CELL RESEARCH
(2023)
Article
Cell Biology
Tanvi Sinha, Kelly Lammerts van Bueren, Diane E. Dickel, Ivana Zlatanova, Reuben Thomas, Carlos O. Lizama, Shan-Mei Xu, Ann C. Zovein, Kohta Ikegami, Ivan P. Moskowitz, Katherine S. Pollard, Len A. Pennacchio, Brian L. Black
Summary: This study investigates the initiation of gene-regulatory networks (GRNs) for endothelial and erythropoietic development by analyzing a hypomorphic Etv2 mutant. The researchers find that Etv2 has different threshold requirements for initiating the downstream GRNs for each lineage, directly regulating the endothelial GRN and indirectly activating Tal1 for erythropoietic development.
Article
Cell Biology
Enakshi Sinniah, Zhixuan Wu, Sophie Shen, Marina Naval-Sanchez, Xiaoli Chen, Junxian Lim, Abbigail Helfer, Abishek Iyer, Jiahui Tng, Andrew J. Lucke, Robert C. Reid, Meredith A. Redd, Christian M. Nefzger, David P. Fairlie, Nathan J. Palpant
Summary: This study reveals the important roles of HDAC1 and HDAC3 in the differentiation of human pluripotent stem cells into mesendodermal and cardiac progenitors, through chemical perturbation and single-cell RNA sequencing analysis.
Article
Computer Science, Information Systems
Woubshet Behutiye, Pilar Rodriguez, Markku Oivo
Summary: In agile software development, quality requirements often lack detailed specifications and documentation. This study developed the Agile QR-Doc QR documentation guidelines to support quality requirements documentation. Through surveys and discussions with software practitioners, the guidelines were validated and found to be relevant, understandable, and useful in supporting QR documentation in agile software development.
Article
Multidisciplinary Sciences
Karla T. Falcon, Kristin E. N. Watt, Soma Dash, Ruonan Zhao, Daisuke Sakai, Emma L. Moore, Sharien Fitriasari, Melissa Childers, Mihaela E. Sardiu, Selene Swanson, Dai Tsuchiya, Jay Unruh, George Bugarinovic, Lin Li, Rita Shiang, Annita Achilleos, Jill Dixon, Michael J. Dixon, Paul A. Trainor
Summary: This study investigates the importance of ribosomal RNA (rRNA) transcription in craniofacial development and the implications of disruptions in this process. The researchers discovered that high expression of Pol I subunits in neuroepithelium and neural crest cells (NCCs) sustains elevated rRNA transcription, supporting the high levels of protein translation in these cells. However, disruptions in rRNA synthesis in NCCs can lead to p53 protein accumulation, NCC apoptosis, and craniofacial anomalies. Compound mutations in Pol I subunits further exacerbate these anomalies. Mechanistically, diminished rRNA synthesis causes an imbalance between rRNA and ribosomal proteins, affecting the binding of these proteins with Mdm2 and p53. This study highlights the critical role of rRNA transcription in craniofacial development and its specific sensitivities to disruptions in certain congenital craniofacial disorders.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Alastair J. Kirby, Thomas Palmer, Richard J. Mead, Ronaldo M. Ichiyama, Samit Chakrabarty
Summary: This study quantified the survival of alpha-MNs and gamma-MNs in the spinal cord segments of SOD1(G93A) mice and observed a caudal-rostral progression of alpha-MN and corticospinal tract degeneration. This suggests that alpha-MNs and the corticospinal pathway in the lumbar spinal cord are more susceptible to degeneration in SOD1(G93A) mice.
Article
Immunology
Hugo Akerstrand, Elena Boldrin, Giorgia Montano, Stijn Vanhee, Karin Olsson, Niklas Krausse, Stefano Vergani, Maciej Ciesla, Cristian Bellodi, Joan Yuan
Summary: The LIN28B RNA binding protein plays a crucial role in fetal and neonatal B lymphopoiesis by amplifying the CD19/PI3K/c-MYC pathway and promoting protein synthesis. Its impact on B cell development is stage-dependent, with IL-7 signaling masking its effect on Pro-B cells. Elevated protein synthesis, mediated by Lin28b, is a defining requirement for early-life B cell development. Impaired protein synthesis specifically affects neonatal B lymphopoiesis and the output of B-1a cells.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Cell Biology
Oscar A. Pena, Alexandra Lubin, Jasmine Rowell, Yvette Hoade, Noreen Khokhar, Hanna Lemmik, Christopher Mahony, Phoebe Dace, Chianna Umamahesan, Elspeth M. Payne
Summary: Germline loss or mutation of one copy of the transcription factor GATA2 in humans results in clinical phenotypes affecting hematopoietic, lymphatic, and vascular systems. In this research, zebrafish with two copies of the Gata2 gene were used to investigate the effects of these genes on hematopoiesis during development, revealing unique roles at different stages and a potential redundancy between the two genes. The study also showed defects in the myeloid compartment in adult zebrafish with combined heterozygosity loss, similar to GATA2 loss in humans, adding to our understanding of GATA2 deficiency and its developmental effects.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Fernando C. Baltanas, Rosula Garcia-Navas, Pablo Rodriguez-Ramos, Nuria Calzada, Cristina Cuesta, Javier Borrajo, Rocio Fuentes-Mateos, Andrea Olarte-San Juan, Nerea Vidana, Esther Castellano, Eugenio Santos
Summary: The impact of genetic ablation of SOS1 or SOS2 was evaluated in a murine model of KRAS(G12D)-driven lung adenocarcinoma (LUAD). The study found that SOS1 plays a critical role in the development and survival of KRAS(G12D)-driven LUAD, and its ablation can significantly increase the lifespan of mice and reduce tumor burden and populations of cancer-associated cells in the tumor microenvironment.
NATURE COMMUNICATIONS
(2023)
Article
Developmental Biology
Anna L. F. Assumpcao, Guoping Fu, Deependra K. Singh, Zhanping Lu, Ashley M. Kuehnl, Rene Welch, Irene M. Ong, Renren Wen, Xuan Pan
Summary: YY1, a ubiquitous transcription factor and PcG protein, plays important roles in regulating lymphocyte development and stem cell self-renewal. The PcG function of YY1 is necessary for T and B cell development, but not for myeloid cells. YY1's role in early T cell development involves both PcG-dependent and -independent regulations, promoting cell survival and Notch1 signaling independently.
Article
Multidisciplinary Sciences
Olga Filina, Burak Demirbas, Rik Haagmans, Jeroen S. van Zon
Summary: Maintaining the correct temporal order of cellular events is crucial for animal development. In this study, researchers used time-lapse microscopy to examine the timing of gene expression, cell divisions, and cuticle shedding in individual Caenorhabditis elegans larvae. They found that there was variability in timing between individuals, but this variability followed temporal scaling. Changes in temperature, diet, or genotype also influenced timing, but still adhered to temporal scaling. However, shifting conditions during development disrupted temporal scaling and changed event order in a condition-specific manner.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Shun Saito, Shota Yamamura, Nanami Kohri, Hanako Bai, Masashi Takahashi, Manabu Kawahara
Summary: WW domain-containing transcription regulator 1 (WWTR1) is a key player in the Hippo pathway and plays a crucial role in the differentiation of trophectoderm (TE) cells during preimplantation development. Knockdown of WWTR1 resulted in reduced TE cell numbers and expression of TE-specific genes in bovine embryos, highlighting the importance of WWTR1 in TE cell development.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Francesca Gabanella, Annalisa Onori, Cinzia Pisani, Marco Fiore, Giampiero Ferraguti, Andrea Colizza, Marco de Vincentiis, Marco Ceccanti, Maurizio Inghilleri, Nicoletta Corbi, Claudio Passananti, Maria Grazia Di Certo
Summary: The deficiency of SMN protein causes SMA, a rare neuromuscular disease affecting various organs. SMN was found to associate with ABCA1 mRNA and impact its subcellular distribution. Cholesterol accumulation was observed in SMN-depleted human fibroblasts, suggesting dysregulated cholesterol efflux as a contributing factor in SMA pathophysiology.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Letter
Clinical Neurology
Yousef Salimpour, Zhikui Wei, Phan Q. Duy, William S. Anderson
Letter
Clinical Neurology
Yousef Salimpour, Kelly A. Mills, Zhikui Wei, Phan Q. Duy, William S. Anderson
Letter
Biology
Phan Q. Duy, Samer Hattar
JOURNAL OF BIOLOGICAL RHYTHMS
(2017)
Article
Neurosciences
Thi Hao Le, Phan Q. Duy, Min An, Jared Talbot, Chitra C. Iyer, Marc Wolman, Christine E. Beattie
JOURNAL OF NEUROSCIENCE
(2017)
Article
Neurosciences
Phan Q. Duy, Michael A. Berberoglu, Christine E. Beattie, Charles W. Hall
Article
Neurosciences
Dejan B. Budimirovic, Smiljana Cvjetkovic, Zoran Bukumiric, Phan Q. Duy, Dragana Protic
FRONTIERS IN NEUROSCIENCE
(2018)
Article
Clinical Neurology
Serban Negoita, Phan Q. Duy, Uma V. Mahajan, William S. Anderson
JOURNAL OF CLINICAL NEUROSCIENCE
(2019)
Article
Developmental Biology
Jared C. Talbot, Emily M. Teets, Dhanushika Ratnayake, Phan Q. Duy, Peter D. Currie, Sharon L. Amacher
Editorial Material
Biochemistry & Molecular Biology
Phan Q Duy, Charuta G. Furey, Kristopher T. Kahle
TRENDS IN MOLECULAR MEDICINE
(2019)
Article
Neurosciences
August A. Allocco, Sheng Chih Jin, Phan Q. Duy, Charuta G. Furey, Xue Zeng, Weilai Dong, Carol Nelson-Williams, Jason K. Karimy, Tyrone DeSpenza, Le T. Hao, Benjamin Reeves, Shozeb Haider, Murat Gunel, Richard P. Lifton, Kristopher T. Kahle
FRONTIERS IN CELLULAR NEUROSCIENCE
(2019)
Article
Neurosciences
Phan Q. Duy, Serban Negoita, Uma V. Mahajan, Nicholas S. Diab, Ank Agarwal, Trisha Gupte, Manish D. Paranjpe, William S. Anderson
TRANSLATIONAL NEUROSCIENCE
(2019)
Article
Neurosciences
Phan Q. Duy, Anirudh Sreekrishnan, Wyatt David, Manish D. Paranjpe, Ishan Paranjpe, Amar Sheth, Batur Gultekin, Kevin N. Sheth
TRANSLATIONAL NEUROSCIENCE
(2019)
Article
Medicine, Research & Experimental
Min Liu, Manish D. Paranjpe, Xin Zhou, Phan Q. Duy, Manu S. Goyal, Tammie L. S. Benzinger, Jie Lu, Rongfu Wang, Yun Zhou
Review
Biology
Phan Q. Duy, William S. Anderson
YALE JOURNAL OF BIOLOGY AND MEDICINE
(2018)
Article
Neurosciences
Phan Q. Duy, Dejan B. Budimirovic
TRANSLATIONAL NEUROSCIENCE
(2017)