Article
Biophysics
Autumn A. Anthony, Osman Sahin, Murat Kaya Yapici, Daniel Rogers, Aurelia R. Honerkamp-Smith
Summary: This study systematically measured the coefficient of interleaflet friction in supported lipid membranes using a microfluidic technique. The results showed that this technique provides reproducible measurements of the friction coefficient and is sensitive enough to detect differences in friction between different lipid membranes. Adding cholesterol significantly increased interleaflet friction. Additionally, fluid shear stress was found to reversibly induce the gel phase in supported lipid bilayers close to the gel-transition temperature.
BIOPHYSICAL JOURNAL
(2022)
Article
Biochemistry & Molecular Biology
Anindya Chowdhury, Sreeja Sasidharan, Pinchu Xavier, P. Viswanath, V. A. Raghunathan
Summary: The study reveals a significant effect of acidic pH on the phase behavior of zwitterionic lipids, leading to an increase in main transition temperature and observation of an unfilled gel phase at low pH. Headgroup dehydration at low pH also results in the formation of DMPC crystallites.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2021)
Article
Chemistry, Physical
Itay Schachter, Riku O. Paananen, Balazs Fabian, Piotr Jurkiewicz, Matti Javanainen
Summary: Through atomistic molecular dynamics simulations, differential scanning calorimetry, and fluorescence spectroscopy on L-o phase membranes, it was found that a stiff, hexagonally packed lipid structure in the L-o phase at room temperature excludes raft-associated proteins, but upon heating, this structure melts, leading to preferential solvation of proteins by order-preferring lipids.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2022)
Article
Biochemistry & Molecular Biology
Nathan E. Thomas, Wei Feng, Katherine A. Henzler-Wildman
Summary: This study presents a new method for measuring transport stoichiometry using solid supported membrane electrophysiology (SSME), which greatly improves throughput and allows verification of specific transport ratios for various ion-coupled transporters.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Alexandra L. Martin, Philip N. Jemmett, Thomas Howitt, Mary H. Wood, Andrew W. Burley, Liam R. Cox, Timothy R. Dafforn, Rebecca J. L. Welbourn, Mario Campana, Maximilian W. A. . Skoda, Joseph J. Thompson, Hadeel Hussain, Jonathan L. Rawle, Francesco Carla, Christopher L. Nicklin, Thomas Arnold, Sarah L. Horswell
Summary: The effect of lipid composition on models of mammalian cell membranes has been investigated. The presence of anionic lipid in zwitterionic lipid bilayers leads to changes in bilayer response and solvation. A specific range of anionic lipid content (10-35 mol %) provides optimal properties for bilayers, explaining the tendency for cell membranes to contain this amount of anionic lipid.
Article
Chemistry, Multidisciplinary
Alexandra L. Martin, Philip N. Jemmett, Thomas Howitt, Mary H. Wood, Andrew W. Burley, Liam R. Cox, Timothy R. Dafforn, Rebecca J. L. Welbourn, Mario Campana, Maximilian W. A. Skoda, Joseph J. Thompson, Hadeel Hussain, Jonathan L. Rawle, Francesco Carla, Christopher L. Nicklin, Thomas Arnold, Sarah L. Horswell
Summary: The effect of lipid composition on models of the inner leaflet of mammalian cell membranes has been investigated. It was found that incorporating a small quantity of the anionic lipid dimyristoylphosphatidylserine (DMPS) into zwitterionic dimyristoylphosphatidylethanolamine (DMPE) bilayers resulted in significant changes in bilayer response to an applied electric field. Optimum bilayer properties were observed in the range of 10-35 mol% DMPS, which may explain the tendency for the DMPS content of cell membranes to fall within this range.
Article
Chemistry, Physical
Massimiliano Galluzzi, Lorenzo Marfori, Stefania Asperti, Alessandro De Vita, Matteo Giannangeli, Alessandro Caselli, Paolo Milani, Alessandro Podesta
Summary: Research has shown that Ionic Liquids (ILs) can restructure lipid bilayers by forming new ILs/lipid complexes, leading to distinctive morphological features such as increased area and roughness. Additionally, IL exposure can disrupt the structural ordering and rigidity of membranes, as evidenced by changes in breakthrough force, Young's modulus, and area stretching modulus. AFM analysis also detected alterations in the electrostatic double-layer at the membrane surface, indicating a progressive stratification of cations on the lipid membrane.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2022)
Article
Chemistry, Multidisciplinary
Aoon Rizvi, Justin T. Mulvey, Joseph P. Patterson
Summary: The study investigates the formation of block-copolymer-supported bilayers using LP-TEM, revealing two formation pathways involving liquid droplets or vesicles as intermediates. The results suggest that bilayer assembly methods via liquid droplet intermediates are beneficial for forming pristine supported bilayers, and supported bilayers inside liquid cells may be useful for imaging membrane interactions with proteins and nanoparticles in the future.
Article
Chemistry, Physical
Simon Christ, Thomas Litschel, Petra Schwille, Reinhard Lipowsky
Summary: The study investigates shape oscillations of giant unilamellar vesicles (GUVs) caused by the Min protein system, where MinD and MinE proteins periodically attach to and detach from the membrane, driven by ATP hydrolysis. The oscillations can be understood in terms of spontaneous curvature changing periodically, with the vesicle shape transforming from symmetric to asymmetric and back during each cycle. The radius of the narrow membrane neck connecting the subcompartments undergoes periodic oscillations with an average time period of 56 seconds.
Article
Chemistry, Multidisciplinary
Rachel J. Goodband, Colin D. Bain, Margarita Staykova
Summary: This study compares the fusion of giant lipid and block-copolymer vesicles on different substrates and characterizes the properties of membrane patches and the formation of continuous supported membranes. Polymer membranes have lower fluidity and adjacent patches fail to fuse spontaneously, possibly due to hydration repulsion forces, which can be weakened by increasing the temperature.
Article
Chemistry, Multidisciplinary
J. Tucker Andrews, Kirstyn E. Baker, Jacob T. Handloser, Natalie Bridges, Alexis A. Krone, Peter J. N. Kett
Summary: This study used a quartz crystal microbalance to investigate the effect of salt on the formation of phosphatidylcholine SLBs on a silicon dioxide surface, finding that the influence of different cations on SLB formation varies at different salt concentrations.
Article
Chemistry, Physical
Volker S. Urban, Raquel L. Lieberman, Wellington C. Leite, Yuqi Wu, Sai Venkatesh Pingali
Summary: This study investigates the impact of the single chain detergent DDM on the morphology of bicelles. DDM is able to reshape the bicelles at different temperatures, which is crucial for understanding lipid membrane self-organization and optimizing lipid mimics in membrane biology research.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2022)
Article
Biochemistry & Molecular Biology
Yanyun Lin, Xingjuan Wu, Kaicheng Wang, Sen Shang, Yubin Gong, Hongwei Zhao, Dai Wu, Peng Zhang, Xiaoyun Lu
Summary: This study examined the THz spectrum of several phospholipid components in biological membranes and found different resonant absorption patterns. The absorption peak at approximately 3.0 THz of DSPE and DPPC was confirmed to originate from a collective vibration of their hydrophobic tails. Additionally, irradiation at 3.1 THz significantly enhanced cell membrane fluidity and improved phagocytosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Ons Hmam, Antonella Badia
Summary: The surface-confined redox reaction triggers the formation of high surface coverage bilayers on gold surfaces. This method is rapid, compatible with various charged phospholipids, and is not affected by the phase state of the phospholipids.
ADVANCED MATERIALS INTERFACES
(2022)
Article
Chemistry, Physical
Daniel J. Kozuch, Frank H. Stillinger, Pablo G. Debenedetti
Summary: The addition of trehalose affects the phase transition and structure of lipid membranes, increasing disorder in the membrane; Both coarse-grained and all-atom simulations show that trehalose has similar effects on lipid membranes at low temperatures.
JOURNAL OF PHYSICAL CHEMISTRY B
(2021)
Article
Chemistry, Inorganic & Nuclear
Andrea Mulas, Yasmine Willener, James Carr-Smith, Kevin M. Joly, Louise Male, Christopher J. Moody, Sarah L. Horswell, Huy V. Nguyen, James H. R. Tucker
DALTON TRANSACTIONS
(2015)
Article
Chemistry, Inorganic & Nuclear
Carlotta Figliola, Louise Male, Sarah L. Horswell, Richard S. Grainger
EUROPEAN JOURNAL OF INORGANIC CHEMISTRY
(2015)
Article
Chemistry, Inorganic & Nuclear
Jack B. A. Davis, Sarah L. Horswell, Laurent Piccolo, Roy L. Johnston
JOURNAL OF ORGANOMETALLIC CHEMISTRY
(2015)
Article
Chemistry, Inorganic & Nuclear
Jonathan L. Kedge, Huy V. Nguyen, Zahra Khan, Louise Male, Media K. Ismail, Holly V. Roberts, Nikolas J. Hodges, Sarah L. Horswell, Youcef Mehellou, James H. R. Tucker
EUROPEAN JOURNAL OF INORGANIC CHEMISTRY
(2017)
Article
Chemistry, Physical
Jack B. A. Davis, Sarah L. Horswell, Roy L. Johnston
JOURNAL OF PHYSICAL CHEMISTRY C
(2016)
Article
Chemistry, Multidisciplinary
Jean-Louis H. A. Duprey, James Carr-Smith, Sarah L. Horswell, Jaroslaw Kowalski, James H. R. Tucker
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2016)
Article
Materials Science, Multidisciplinary
Benjamin P. de Laune, Frank J. Berry, Jose F. Marco, Sarah L. Horswell, Colin Greaves
JOURNAL OF MATERIALS CHEMISTRY C
(2016)
Article
Electrochemistry
J. P. Melo, E. N. Schulz, C. Morales-Verdejo, S. L. Horswell, M. B. Camarada
INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE
(2017)
Article
Chemistry, Analytical
Elena Madrid, Sarah L. Horswell
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2018)
Article
Chemistry, Multidisciplinary
Luca Pisciottani, Maxime Douarre, Brigitte Bibal, Clotilde Davies, Holly Roberts, Brice Kauffmann, Sarah L. Horswell, James H. R. Tucker, Nathan D. McClenaghan
SUPRAMOLECULAR CHEMISTRY
(2018)
Article
Chemistry, Physical
Heider A. Hussein, Mansi Gao, Yiyun Hou, Sarah L. Horswell, Roy L. Johnston
ZEITSCHRIFT FUR PHYSIKALISCHE CHEMIE-INTERNATIONAL JOURNAL OF RESEARCH IN PHYSICAL CHEMISTRY & CHEMICAL PHYSICS
(2019)
Article
Chemistry, Multidisciplinary
Spyridon Varlas, Robert Keogh, Yujie Xie, Sarah L. Horswell, Jeffrey C. Foster, Rachel K. O'Reilly
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2019)
Article
Biochemistry & Molecular Biology
Media K. Ismail, Zahra Khan, Marium Rana, Sarah L. Horswell, Louise Male, Huy V. Nguyen, Alessio Perotti, Isolda Romero-Canelon, Edward A. Wilkinson, Nikolas J. Hodges, James H. R. Tucker
Article
Chemistry, Inorganic & Nuclear
Media K. Ismail, Katie A. Armstrong, Samantha L. Hodder, Sarah L. Horswell, Louise Male, Huy V. Nguyen, Edward A. Wilkinson, Nikolas J. Hodges, James H. R. Tucker
DALTON TRANSACTIONS
(2020)
Review
Chemistry, Physical
T. Albrecht, S. Horswell, L. K. Allerston, N. V. Rees, P. Rodriguez
CURRENT OPINION IN ELECTROCHEMISTRY
(2018)