Staphylococcus epidermidis Affinity for Fibrinogen-Coated Surfaces Correlates with the Abundance of the SdrG Adhesin on the Cell Surface

Staphylococcus epidermidis is a world-leading pathogen in: healthcare facilities, mainly causing medical device-associated infections. These nosocomial diseases often result in complications such as bacteremia, fibrosis, peritonitis. The Virulence of S. epidermidis relies on its ability to Colonize Surfaces and;develop thereupon in the form of biofilms: Bacterial adherence on biomaterials, usually covered with plasma proteins after implantation, is a critical step leading to biofilm infections. The cell surface protein SdrG mediates adhesion of S. epidermidis to fibrinogen (Fg) through a specific dock, lock, and latch mechanism, which results in greatly stabilized protein ligand complexes. Here, we combine;single-molecule, single-cell, and whole population assays to investigate the extent to which the surface density of SdrG determines, the ability of S. opidermidis clinical strains HB, ATCC 35984, and ATCC 12228 to bind to Fg-coated Surfaces. Strains that showed enhanced adhesion on Fg-coated polydimethylsiloxane (PDMS) were characterized by increased amounts of SdrG proteins on the cell surface, as observed by single-molecule analysis. Consistent with previous, reports showing increased expression of SdrG following in vivo exposure, this work provides direct evidence that abundance of SdrG on the cell surface of S. epidermidis strains dramatically improves their ability to bind to Fg-coated implanted medical devices.