Article
Chemistry, Multidisciplinary
Xing Zhu, Chenxi Du, Bo Gao, Bin He
Summary: This study developed a robust bionic jellyfish-carbonic anhydrase complex (Bj-CA) for efficient CO2 capture.
Article
Multidisciplinary Sciences
Kevin M. Hines, Vishalsingh Chaudhari, Kristen N. Edgeworth, Thomas G. Owens, Maureen R. Hanson
Summary: The stromal CAs 13-CA1 and 13-CA5 in tobacco play significant roles in leaf development and CO2 fixation, but they are not necessary for maximal photosynthetic rates in C3 plant tobacco.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Chemistry, Physical
Gediminas Skvarnavicius, Zigmantas Toleikis, Daumantas Matulis, Vytautas Petrauskas
Summary: A complete thermodynamic description of protein-ligand binding includes parameters related to pressure and temperature, which are often neglected due to the lack of routine methods for their determination. The fluorescent pressure shift assay (FPSA) offers a universal approach to determine protein-ligand binding volumes by studying pressure-induced protein unfolding and its stabilization by a ligand. This method allows for the determination of protein-ligand binding volumes that would otherwise be impossible due to the high pressures required for protein unfolding.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2022)
Review
Chemistry, Medicinal
Shubham Kumar, Sandeep Rulhania, Shalini Jaswal, Vikramdeep Monga
Summary: Carbonic anhydrase is an important enzyme involved in various physiological and pathological processes, with 16 different isoforms in humans. Inhibitors targeting different isoforms of carbonic anhydrase have clinical applications in treating various diseases, but current drugs lack selectivity leading to undesired side effects. Efforts are being made to develop novel isoform-selective inhibitors of carbonic anhydrase for therapeutic implications.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Engineering, Environmental
Peijing Shao, Zheng He, Yutao Hu, Yao Shen, Shihan Zhang, Yanan Yu
Summary: Enzyme immobilization using zeolitic imidazolate frameworks, particularly ZIF-8, has been shown to improve the stability and activity levels of carbonic anhydrase (CA) enzymes for flue gas CO2 capture.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Spectroscopy
Xiaojuan Wang, Zhixiong Zhang, Zhenzhen Liu, Xiqi Ma, Qi Dai, Xiaoqiang Wang, Baosheng Ge, Hua He, Fang Huang
Summary: This study systematically investigated the interactions between ultrasmall graphene quantum dots (GQDs) and biomacromolecules using carbonic anhydrase (CA) as a model protein. The results showed that GQDs could quench the intrinsic fluorescence of CA in a static binding mode driven by van der Waal interactions and hydrogen bonding. Additionally, the binding of GQDs induced slight conformational changes of CA and resulted in the reduction of its biological functions.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Multidisciplinary Sciences
Hamada H. Amer, Essam Hassan Eldrehmy, Salama Mostafa Abdel-Hafez, Youssef Saeed Alghamdi, Magdy Yassin Hassan, Saad H. Alotaibi
Summary: A series of nucleosides, moieties, and Schiff bases were synthesized from sulfadimidine in this study. After purification using chromatographic techniques, the compounds were subjected to molecular docking studies and tested for antibacterial activity against Staphylococcus aureus, E. coli, and Klebsiella pneumoniae. The most effective compounds were identified for each bacterium, with promising antibacterial effects observed in vitro. Further research is needed to explore the in vivo antibacterial effects and therapeutic doses of these compounds.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Xiaojing Huang, Daniel Winter, Dominic. J. J. Glover, Claudiu. T. T. Supuran, William. A. A. Donald
Summary: Carbonic anhydrases (CAs) are metalloenzymes that play important roles in cellular processes and have been implicated in various diseases. Phosphorylation, a common post-translational modification of CAs, can significantly impact their catalytic activity and drug-binding capabilities. This study highlights the potential regulatory role of phosphorylation in CA activity and its effect on small molecule drug binding.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Polymer Science
Chia-Jung Hsieh, Ju-Chuan Cheng, Chia-Jung Hu, Chi-Yang Yu
Summary: The fusion protein R5-SazCA, formed by combining a silica-condensing peptide with the fastest known carbonic anhydrase, showed high efficiency and stability in CO2 sequestration, making it a promising biocatalyst for this important process.
Article
Multidisciplinary Sciences
Joanna Xiuzhu Xu, Md Siddik Alom, Rahul Yadav, Nicholas C. Fitzkee
Summary: The authors develop a residue-based affinity scale to predict protein orientation on gold nanoparticles. The scale takes into account the steric considerations for hydrophobic residues and the electrostatic interactions for hydrophilic residues. Using this model, the accessibility of active sites on proteins on nanoparticles can be predicted.
NATURE COMMUNICATIONS
(2022)
Article
Immunology
Xianqin Song, Yu Song, Quanping Ma, Kehua Fang, Xiaotian Chang
Summary: This study found that M1 macrophages can increase the expression of CA1 and CA2 to promote atherosclerotic calcification in vascular smooth muscle cells (VSMCs) by secreting TNF-a.
JOURNAL OF INFLAMMATION RESEARCH
(2023)
Article
Chemistry, Multidisciplinary
Hongxu Liu, Jenna Westley, S. Thayumanavan
Summary: A protein binding-induced supramolecular dissociation strategy has been developed for protein sensing and quantification, showing excellent selectivity and quantification accuracy due to specific ligand-protein binding. The ratiometric approach is immune to interference from extrinsic quenchers while allowing for protein specificity.
CHEMICAL COMMUNICATIONS
(2021)
Article
Environmental Sciences
Shizheng Xiang, Yiqiang Li, Wanying Wang, Biao Zhang, Wenyu Shi, Jia Zhang, Fuyang Huang, Fei Liu, Xiangyu Guan
Summary: This study delves into the microbiome of a karst river contaminated with antibiotics, revealing the diversity of carbon fixation pathways in different ecological clusters and their correlation with antibiotics. Long-term antibiotic contamination alters microbial ecological adaptation strategies, with carbon sequestration functions playing a significant role in regulating environmental factors and microbial networks.
ENVIRONMENTAL POLLUTION
(2021)
Article
Chemistry, Multidisciplinary
Sebastian Oehler, Laura Lucaroni, Francesca Migliorini, Abdullah Elsayed, Luca Prati, Sara Puglioli, Mattia Matasci, Kristina Schira, Jorg Scheuermann, Denis Yudin, Min Jia, Nenad Ban, Dave Bushnell, Roger Kornberg, Samuele Cazzamalli, Dario Neri, Nicholas Favalli, Gabriele Bassi
Summary: DNA-encoded chemical libraries (DELs) with stereo-defined elements facilitate the discovery of specific ligands for discriminating between closely related protein targets. We synthesized a DEL with 3,735,936 members using stereo-defined central scaffolds and identified potent isozyme-selective ligands against multiple protein targets. Some of these ligands demonstrated tumor-selective targeting in vitro and in vivo. Constructing DELs with stereo-defined elements contributes to high library productivity and ligand selectivity.
Article
Chemistry, Medicinal
Samar A. El-Kalyoubi, Ehab S. Taher, Tarek S. Ibrahim, Mohammed Farrag El-Behairy, Amany M. M. Al-Mahmoudy
Summary: A series of quinoline-uracil hybrids were synthesized and studied for their inhibitory activity and selectivity against multiple enzyme isoforms. Compound 101 showed the best performance in terms of inhibitory activity and selectivity.